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The map label for this gene is leuA

Identifier: 119944002

GI number: 119944002

Start: 270302

End: 271861

Strand: Reverse

Name: leuA

Synonym: Ping_0216

Alternate gene names: 119944002

Gene position: 271861-270302 (Counterclockwise)

Preceding gene: 119944006

Following gene: 119944001

Centisome position: 5.96

GC content: 42.37

Gene sequence:

>1560_bases
ATGTCCGATCAAGTAATAATTTTCGACACGACACTTCGTGATGGAGAGCAGGCATTAAGTGCTAGCCTGACCGTTAAAGA
AAAACTACAGATCGCATTTGCTCTCGAACGTTTAGGTGTTGATGTAATGGAGGTTGGTTTTCCGATCTCCTCACCCGGTG
ATTTTGAGTCGGTAAAAACCATTGCCAGAGAAATTAAAAATAGCCGTGTCTGTGGATTATCCCGGGCATTGCCAAAAGAT
ATTGATGCTGCGTGGAATGCATTAAAAGGGGCGGATGCCTTCCGTATCCATACTTTTATTTCAACGTCCACTATTCATGT
AGAAAATAAATTAAAACGCACCTTTGATGCGGTCTTAGAGATGGGTATTAATGCAGTAAAACATGCACGTAATTATACCG
ATGATGTTGAATTTTCCTGTGAAGATGCCGGTCGTACCCCAATCGACAACTTATGCCGGATCGTGGAGGAAGCCATTAAA
GCGGGCGCAACCACCATTAACATTCCGGATACCGTGGGTTATACCTATCCGAGTGAATTTGGCGGCATTATCAAAACCTT
ATTTAATCGCGTACCTAATATAGATCAAGCCATTATTTCAGTGCATTGTCATGACGATTTAGGTATGTCCGTGGCCAACT
CGATTACCGCGGTTGAAAATGGTGCGCGTCAAATTGAATGCACTATGAATGGTCTTGGTGAACGTGCAGGGAACTGCTCA
CTTGAAGAAGTGGCCATGATTTTACAGACCCGCAAAGATAAGTTGGGATTTACTACCAATGTCAATCCCATGGAAATTTC
GCGTACCAGCCAGCTGGTCAGCCAACTGTGTAATATGCCCATTCAAGCAAACAAGGCGATTGTCGGTGCTAATGCGTTTT
CACACTCCTCGGGTATTCACCAGGATGGTGTTCTAAAATCACAGAATACCTATGAGATTATGACCCCTGAAAGTGTCGGT
ATTTCAACCAATAAGTTAAATTTAACTTCCCGCTCCGGCCGCCATGTTATCCAGCATCGTATGCAGGAATTAGGCTACCG
TGATACAGATTATGACCTGGAACAGTTGTATGCTAGTTTTGTAGAACTCGCAGATAAAAAAGGACAAGTCTTCGATTATG
ATTTAGAAGCCTTAATGTTCTTTAATAAAGTCGATTCGGATCCGCAGCACTACAGACTGGAAAGTATTAATGTCCAGTCC
GGTTCAGGATTAGTTGCGACGGCCACCATTGTGATGAGTATTGGTGAGGATAAAAAAGTAGTTGAAGCAGCTACTGGGAA
TGGGCCTATTGATGCTGCATACCAATGTCTAATGCGGATTTCAGGTTTAGATATTAATATGGATGATTACCATATTAATG
CAAAAGGTGCCGGTAAAGATGCACTTGGCCAGGTCGATATTGTCGCCACCTACAACGGTCAGAAATTTCACGGTTTAGGC
TTATCAACGGATATTATAGAATCATCTACTAAAGCGATGGTGCATGTTATGAATCATATCCATTTGGCAAAAGCCGTTGC
CATCGAAAAAGAACAGCTTATTCATATTGATCAGGTTTAA

Upstream 100 bases:

>100_bases
GGCTGAATAAAATCAGTTAAATTTCCAAAAACCCGTGCCCTGCGCGGGTTTTTTTATATCCATAATAACTACATAATATT
AATAAAAAGAGGGATATTTT

Downstream 100 bases:

>100_bases
TGATACTCAAATTACTTGAGAATGCCGTCATAAGAACCATTAAGTAGTTTGGGTATATAGTTTCAGATGATTTGCGAATT
CATAAGGTAACCAATGGGTT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MSDQVIIFDTTLRDGEQALSASLTVKEKLQIAFALERLGVDVMEVGFPISSPGDFESVKTIAREIKNSRVCGLSRALPKD
IDAAWNALKGADAFRIHTFISTSTIHVENKLKRTFDAVLEMGINAVKHARNYTDDVEFSCEDAGRTPIDNLCRIVEEAIK
AGATTINIPDTVGYTYPSEFGGIIKTLFNRVPNIDQAIISVHCHDDLGMSVANSITAVENGARQIECTMNGLGERAGNCS
LEEVAMILQTRKDKLGFTTNVNPMEISRTSQLVSQLCNMPIQANKAIVGANAFSHSSGIHQDGVLKSQNTYEIMTPESVG
ISTNKLNLTSRSGRHVIQHRMQELGYRDTDYDLEQLYASFVELADKKGQVFDYDLEALMFFNKVDSDPQHYRLESINVQS
GSGLVATATIVMSIGEDKKVVEAATGNGPIDAAYQCLMRISGLDINMDDYHINAKGAGKDALGQVDIVATYNGQKFHGLG
LSTDIIESSTKAMVHVMNHIHLAKAVAIEKEQLIHIDQV

Sequences:

>Translated_519_residues
MSDQVIIFDTTLRDGEQALSASLTVKEKLQIAFALERLGVDVMEVGFPISSPGDFESVKTIAREIKNSRVCGLSRALPKD
IDAAWNALKGADAFRIHTFISTSTIHVENKLKRTFDAVLEMGINAVKHARNYTDDVEFSCEDAGRTPIDNLCRIVEEAIK
AGATTINIPDTVGYTYPSEFGGIIKTLFNRVPNIDQAIISVHCHDDLGMSVANSITAVENGARQIECTMNGLGERAGNCS
LEEVAMILQTRKDKLGFTTNVNPMEISRTSQLVSQLCNMPIQANKAIVGANAFSHSSGIHQDGVLKSQNTYEIMTPESVG
ISTNKLNLTSRSGRHVIQHRMQELGYRDTDYDLEQLYASFVELADKKGQVFDYDLEALMFFNKVDSDPQHYRLESINVQS
GSGLVATATIVMSIGEDKKVVEAATGNGPIDAAYQCLMRISGLDINMDDYHINAKGAGKDALGQVDIVATYNGQKFHGLG
LSTDIIESSTKAMVHVMNHIHLAKAVAIEKEQLIHIDQV
>Mature_518_residues
SDQVIIFDTTLRDGEQALSASLTVKEKLQIAFALERLGVDVMEVGFPISSPGDFESVKTIAREIKNSRVCGLSRALPKDI
DAAWNALKGADAFRIHTFISTSTIHVENKLKRTFDAVLEMGINAVKHARNYTDDVEFSCEDAGRTPIDNLCRIVEEAIKA
GATTINIPDTVGYTYPSEFGGIIKTLFNRVPNIDQAIISVHCHDDLGMSVANSITAVENGARQIECTMNGLGERAGNCSL
EEVAMILQTRKDKLGFTTNVNPMEISRTSQLVSQLCNMPIQANKAIVGANAFSHSSGIHQDGVLKSQNTYEIMTPESVGI
STNKLNLTSRSGRHVIQHRMQELGYRDTDYDLEQLYASFVELADKKGQVFDYDLEALMFFNKVDSDPQHYRLESINVQSG
SGLVATATIVMSIGEDKKVVEAATGNGPIDAAYQCLMRISGLDINMDDYHINAKGAGKDALGQVDIVATYNGQKFHGLGL
STDIIESSTKAMVHVMNHIHLAKAVAIEKEQLIHIDQV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=511, Percent_Identity=66.9275929549902, Blast_Score=719, Evalue=0.0,
Organism=Escherichia coli, GI1786548, Length=300, Percent_Identity=26, Blast_Score=63, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6320019, Length=386, Percent_Identity=27.2020725388601, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6320071, Length=361, Percent_Identity=27.4238227146814, Blast_Score=130, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6324225, Length=540, Percent_Identity=25.3703703703704, Blast_Score=121, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6324682, Length=540, Percent_Identity=24.4444444444444, Blast_Score=115, Evalue=1e-26,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU1_PSYIN (A1SRG8)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_941682.1
- ProteinModelPortal:   A1SRG8
- SMR:   A1SRG8
- STRING:   A1SRG8
- GeneID:   4627154
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_0216
- eggNOG:   COG0119
- HOGENOM:   HBG701974
- OMA:   DQAIISV
- PhylomeDB:   A1SRG8
- ProtClustDB:   PRK00915
- BioCyc:   PING357804:PING_0216-MONOMER
- HAMAP:   MF_01025_B
- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00917
- TIGRFAMs:   TIGR00973

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer; SSF110921 2-isopropylmalate_synth_dimer

EC number: =2.3.3.13

Molecular weight: Translated: 56775; Mature: 56644

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDQVIIFDTTLRDGEQALSASLTVKEKLQIAFALERLGVDVMEVGFPISSPGDFESVKT
CCCCEEEEECCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHH
IAREIKNSRVCGLSRALPKDIDAAWNALKGADAFRIHTFISTSTIHVENKLKRTFDAVLE
HHHHHHCCCCCCHHHCCCHHHHHHHHHHCCCCCEEEEEEEECEEEEHHHHHHHHHHHHHH
MGINAVKHARNYTDDVEFSCEDAGRTPIDNLCRIVEEAIKAGATTINIPDTVGYTYPSEF
HHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHH
GGIIKTLFNRVPNIDQAIISVHCHDDLGMSVANSITAVENGARQIECTMNGLGERAGNCS
HHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEHHHHCCCCCCCC
LEEVAMILQTRKDKLGFTTNVNPMEISRTSQLVSQLCNMPIQANKAIVGANAFSHSSGIH
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCC
QDGVLKSQNTYEIMTPESVGISTNKLNLTSRSGRHVIQHRMQELGYRDTDYDLEQLYASF
CCCCCCCCCCEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
VELADKKGQVFDYDLEALMFFNKVDSDPQHYRLESINVQSGSGLVATATIVMSIGEDKKV
HHHHHCCCCEEECCHHHHHHHHHCCCCCCCEEEEEEEECCCCCEEEEEEEEEECCCCCCE
VEAATGNGPIDAAYQCLMRISGLDINMDDYHINAKGAGKDALGQVDIVATYNGQKFHGLG
EEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCEEEEEEECCCEEECCC
LSTDIIESSTKAMVHVMNHIHLAKAVAIEKEQLIHIDQV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCC
>Mature Secondary Structure 
SDQVIIFDTTLRDGEQALSASLTVKEKLQIAFALERLGVDVMEVGFPISSPGDFESVKT
CCCEEEEECCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHH
IAREIKNSRVCGLSRALPKDIDAAWNALKGADAFRIHTFISTSTIHVENKLKRTFDAVLE
HHHHHHCCCCCCHHHCCCHHHHHHHHHHCCCCCEEEEEEEECEEEEHHHHHHHHHHHHHH
MGINAVKHARNYTDDVEFSCEDAGRTPIDNLCRIVEEAIKAGATTINIPDTVGYTYPSEF
HHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHH
GGIIKTLFNRVPNIDQAIISVHCHDDLGMSVANSITAVENGARQIECTMNGLGERAGNCS
HHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEHHHHCCCCCCCC
LEEVAMILQTRKDKLGFTTNVNPMEISRTSQLVSQLCNMPIQANKAIVGANAFSHSSGIH
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCC
QDGVLKSQNTYEIMTPESVGISTNKLNLTSRSGRHVIQHRMQELGYRDTDYDLEQLYASF
CCCCCCCCCCEEEECCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
VELADKKGQVFDYDLEALMFFNKVDSDPQHYRLESINVQSGSGLVATATIVMSIGEDKKV
HHHHHCCCCEEECCHHHHHHHHHCCCCCCCEEEEEEEECCCCCEEEEEEEEEECCCCCCE
VEAATGNGPIDAAYQCLMRISGLDINMDDYHINAKGAGKDALGQVDIVATYNGQKFHGLG
EEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCEEEEEEECCCEEECCC
LSTDIIESSTKAMVHVMNHIHLAKAVAIEKEQLIHIDQV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA