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The map label for this gene is spoT [H]

Identifier: 119943990

GI number: 119943990

Start: 255345

End: 257492

Strand: Reverse

Name: spoT [H]

Synonym: Ping_0203

Alternate gene names: 119943990

Gene position: 257492-255345 (Counterclockwise)

Preceding gene: 119943991

Following gene: 119943989

Centisome position: 5.65

GC content: 40.18

Gene sequence:

>2148_bases
ATGTCAATATTTCAACCACTTAAAGATCTTACTGAATCTTACCTTTCAGCGGAGCAATGTGCATTTATTGAACGAGCCTA
TCGCTTTGCTTATCTTGCACATGAAGGTGTTAAACGTGCCAGCGGCGAGCCTTACATTACTCATCCCGTTGCTGTCGCTG
AAATTCTTGCAGGAATGCACCTTGATCATGAAGCAATAGCAGCTGCTTTACTGCATGATGTTCTGGAAGATACCGATGCA
ACCTATCAAGAACTCGCAGATGCATTTAATACTAGCGTAGCGGAACTCGTAGAAGGGGTAACCAAGTTAGATAAAGTAAA
CTTTAGAAACCACAAAGAAGCTCAAGCCGAAAATATTCGCAAAATGCTGATCGCAATGTCCAAAGATTTTCGCGTGATGT
TGATTAAACTTGCGGATCGCGTCCATAACATGCGCACATTAGGTTCACTTCGACAAGATAAACGGCGCCGTATCGCCCAG
GAAACATTTGAAATATTTACGCCTATCGCCGACCGATTGGGTCTTCATAATCTCAAAAATGAATTAGAGCATTTAGCTTT
TAAGGCACAAAACAGAGCACGTTATAATGTCTTAAAAGAGGCAATTAATAAAGCACAGAAGAATCGTAAAGACTTTATCC
AAAATATTTGTGCTGAGATTTCAGATAAACTGCAAACCCTGCCGATGGAAGCAAAAGTAATTGGCCGTCAAAAAGACCTC
TACAGTTTGTATACCAAAATGCGTCAAAAAGGTTGCCAGTTTCATGATGTGATGGATGTCTATGCTTTTCGAATTATCGT
CAAAGACCTTGATAGTTGTTATCGGGCTTTAGGCCAAGTACACCATCTTTATCAACCTAAATTAGGCCACTTTAAAGATT
ATATCGCGATCCCTAAAGCCAATAGTTATCAATCAATACATACTACCCTGCTCACTCATCATGGCGTAAATATTGAAATT
CAAATTCGTACTGAAGATATGGATGAAGTGGCTAAATATGGTATAGCGGCACATTGGGGTTATAAAGAGGGCAGTAATAA
TCAGAATATGACTGCGCAGTTACAAACCCGCAACTGGTTCCAGGGCCTGTCTGATCAGGAAGATCATACAACTAATTCAG
TTTTAATGTTAGAAAATATAAAACAAGAGTTGGGCAAAAATGAAATACATGTTTTGACTCCGCAGGGTAAGATCATTGTC
TTGCCTGCACAGGCAACGCCCGTTGATTTTGCCTATGCACTGCACACCAAAATAGGTCACAGATGTATAGGTACAAAAGT
GGATAACGAGTTTGCCCTGCTGAGTAATAAATTGGAAAGCGGCCAAACCATTGAGATCATCACATCGGATGAAGAACATC
CCTGTGGCGATTGGTTAAATTTTGTTGCCACTCATAAAGCACGCCTCAATATTCGCCAGTACCTGAAACAACTGCCTCAG
GAGCATACAGTGCCAGAGGGCAGACGTTTATTAAAACAGGCACTTGGTAGTACCAATTTAACCGATATTCCGCAAGAAAA
TATCGACAAACTTCTTGCCGATCATCGCCTGAATGATTTTGACAGCCTATTAAATGAAATAGGCAGCGGCAAATTGATGA
GCATTATTGTTGCCCGCCGTTTACGTGGCAATGCCGCTGAATTAACGGAATCTGAGCTCAAACAACAGAACCGGAGAAGT
GCTATCAAAGGCACAGAAGGAATGATGGTTTCCTATGCAAAATGCTGTAAACCCTTACCGGGTGATGCCGTTATTGCACA
TACCAGTCCAGGCAGAGGTTTAGTTGTTCATGCCAAGCATTGCCATAATGTCACTGGGTTTGAAGATGAACTGGATAAAT
ATATTCCCGTTGAGTGGGACATGAATGTGATGAATCAGTTTGAATACTCTAGTGACCTGATTATTATGATGATTAACCAT
AAAGCAGGCCTTGCCCAGCTATTTAATCTAATTTCCAAATGTAATGCCAATGTTGTTGAGATACAAACAACGGAATTACC
CGATCATCTCTATTTAATAAATTTAACACTAGTGGTTATTAACCGGATCCATTTATCCAAAGTAATGCGTCAAATTAAAA
AACTACCCAGTGTTAAAAGTGTTAAACGCAGTAATCAAAAGGACAGATCAAAAAGGCAAATTAATTGA

Upstream 100 bases:

>100_bases
GCAGGATTGCTCTTTATGTTATGGTTCTATATAAAACTTACTAATACTTAATTCTTGAACAACCATCATGGCACACTCAG
TGAGTTAAAAAAGGAAAAAG

Downstream 100 bases:

>100_bases
AATTGTTTAAGCAACTCTCGCTATTATTTATATCGACGGCACTTGTCGCCTGCAGTCTAAAAACAGCTGACAGCATGACA
TCTAAAAACATGATCTCGAA

Product: (p)ppGpp synthetase II and guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 715; Mature: 714

Protein sequence:

>715_residues
MSIFQPLKDLTESYLSAEQCAFIERAYRFAYLAHEGVKRASGEPYITHPVAVAEILAGMHLDHEAIAAALLHDVLEDTDA
TYQELADAFNTSVAELVEGVTKLDKVNFRNHKEAQAENIRKMLIAMSKDFRVMLIKLADRVHNMRTLGSLRQDKRRRIAQ
ETFEIFTPIADRLGLHNLKNELEHLAFKAQNRARYNVLKEAINKAQKNRKDFIQNICAEISDKLQTLPMEAKVIGRQKDL
YSLYTKMRQKGCQFHDVMDVYAFRIIVKDLDSCYRALGQVHHLYQPKLGHFKDYIAIPKANSYQSIHTTLLTHHGVNIEI
QIRTEDMDEVAKYGIAAHWGYKEGSNNQNMTAQLQTRNWFQGLSDQEDHTTNSVLMLENIKQELGKNEIHVLTPQGKIIV
LPAQATPVDFAYALHTKIGHRCIGTKVDNEFALLSNKLESGQTIEIITSDEEHPCGDWLNFVATHKARLNIRQYLKQLPQ
EHTVPEGRRLLKQALGSTNLTDIPQENIDKLLADHRLNDFDSLLNEIGSGKLMSIIVARRLRGNAAELTESELKQQNRRS
AIKGTEGMMVSYAKCCKPLPGDAVIAHTSPGRGLVVHAKHCHNVTGFEDELDKYIPVEWDMNVMNQFEYSSDLIIMMINH
KAGLAQLFNLISKCNANVVEIQTTELPDHLYLINLTLVVINRIHLSKVMRQIKKLPSVKSVKRSNQKDRSKRQIN

Sequences:

>Translated_715_residues
MSIFQPLKDLTESYLSAEQCAFIERAYRFAYLAHEGVKRASGEPYITHPVAVAEILAGMHLDHEAIAAALLHDVLEDTDA
TYQELADAFNTSVAELVEGVTKLDKVNFRNHKEAQAENIRKMLIAMSKDFRVMLIKLADRVHNMRTLGSLRQDKRRRIAQ
ETFEIFTPIADRLGLHNLKNELEHLAFKAQNRARYNVLKEAINKAQKNRKDFIQNICAEISDKLQTLPMEAKVIGRQKDL
YSLYTKMRQKGCQFHDVMDVYAFRIIVKDLDSCYRALGQVHHLYQPKLGHFKDYIAIPKANSYQSIHTTLLTHHGVNIEI
QIRTEDMDEVAKYGIAAHWGYKEGSNNQNMTAQLQTRNWFQGLSDQEDHTTNSVLMLENIKQELGKNEIHVLTPQGKIIV
LPAQATPVDFAYALHTKIGHRCIGTKVDNEFALLSNKLESGQTIEIITSDEEHPCGDWLNFVATHKARLNIRQYLKQLPQ
EHTVPEGRRLLKQALGSTNLTDIPQENIDKLLADHRLNDFDSLLNEIGSGKLMSIIVARRLRGNAAELTESELKQQNRRS
AIKGTEGMMVSYAKCCKPLPGDAVIAHTSPGRGLVVHAKHCHNVTGFEDELDKYIPVEWDMNVMNQFEYSSDLIIMMINH
KAGLAQLFNLISKCNANVVEIQTTELPDHLYLINLTLVVINRIHLSKVMRQIKKLPSVKSVKRSNQKDRSKRQIN
>Mature_714_residues
SIFQPLKDLTESYLSAEQCAFIERAYRFAYLAHEGVKRASGEPYITHPVAVAEILAGMHLDHEAIAAALLHDVLEDTDAT
YQELADAFNTSVAELVEGVTKLDKVNFRNHKEAQAENIRKMLIAMSKDFRVMLIKLADRVHNMRTLGSLRQDKRRRIAQE
TFEIFTPIADRLGLHNLKNELEHLAFKAQNRARYNVLKEAINKAQKNRKDFIQNICAEISDKLQTLPMEAKVIGRQKDLY
SLYTKMRQKGCQFHDVMDVYAFRIIVKDLDSCYRALGQVHHLYQPKLGHFKDYIAIPKANSYQSIHTTLLTHHGVNIEIQ
IRTEDMDEVAKYGIAAHWGYKEGSNNQNMTAQLQTRNWFQGLSDQEDHTTNSVLMLENIKQELGKNEIHVLTPQGKIIVL
PAQATPVDFAYALHTKIGHRCIGTKVDNEFALLSNKLESGQTIEIITSDEEHPCGDWLNFVATHKARLNIRQYLKQLPQE
HTVPEGRRLLKQALGSTNLTDIPQENIDKLLADHRLNDFDSLLNEIGSGKLMSIIVARRLRGNAAELTESELKQQNRRSA
IKGTEGMMVSYAKCCKPLPGDAVIAHTSPGRGLVVHAKHCHNVTGFEDELDKYIPVEWDMNVMNQFEYSSDLIIMMINHK
AGLAQLFNLISKCNANVVEIQTTELPDHLYLINLTLVVINRIHLSKVMRQIKKLPSVKSVKRSNQKDRSKRQIN

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance [H]

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=705, Percent_Identity=50.7801418439716, Blast_Score=736, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=735, Percent_Identity=29.7959183673469, Blast_Score=351, Evalue=1e-97,
Organism=Drosophila melanogaster, GI24650996, Length=139, Percent_Identity=34.5323741007194, Blast_Score=67, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 81426; Mature: 81294

Theoretical pI: Translated: 8.40; Mature: 8.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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EEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHCCCC
>Mature Secondary Structure 
SIFQPLKDLTESYLSAEQCAFIERAYRFAYLAHEGVKRASGEPYITHPVAVAEILAGMH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHHCCC
LDHEAIAAALLHDVLEDTDATYQELADAFNTSVAELVEGVTKLDKVNFRNHKEAQAENIR
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
KMLIAMSKDFRVMLIKLADRVHNMRTLGSLRQDKRRRIAQETFEIFTPIADRLGLHNLKN
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELEHLAFKAQNRARYNVLKEAINKAQKNRKDFIQNICAEISDKLQTLPMEAKVIGRQKDL
HHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHH
YSLYTKMRQKGCQFHDVMDVYAFRIIVKDLDSCYRALGQVHHLYQPKLGHFKDYIAIPKA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHEECCCC
NSYQSIHTTLLTHHGVNIEIQIRTEDMDEVAKYGIAAHWGYKEGSNNQNMTAQLQTRNWF
CCHHHHHHHHHHHCCCEEEEEEECCCHHHHHHCCCCEECCCCCCCCCCCCEEEEEHHHHH
QGLSDQEDHTTNSVLMLENIKQELGKNEIHVLTPQGKIIVLPAQATPVDFAYALHTKIGH
CCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCCCHHHHHHHHHHCC
RCIGTKVDNEFALLSNKLESGQTIEIITSDEEHPCGDWLNFVATHKARLNIRQYLKQLPQ
HHCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
EHTVPEGRRLLKQALGSTNLTDIPQENIDKLLADHRLNDFDSLLNEIGSGKLMSIIVARR
CCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHH
LRGNAAELTESELKQQNRRSAIKGTEGMMVSYAKCCKPLPGDAVIAHTSPGRGLVVHAKH
HCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCEEEEEHH
CHNVTGFEDELDKYIPVEWDMNVMNQFEYSSDLIIMMINHKAGLAQLFNLISKCNANVVE
CCCCCCCHHHHHCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCCEEE
IQTTELPDHLYLINLTLVVINRIHLSKVMRQIKKLPSVKSVKRSNQKDRSKRQIN
EEECCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]