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The map label for this gene is htrB [H]

Identifier: 119943975

GI number: 119943975

Start: 238361

End: 239281

Strand: Reverse

Name: htrB [H]

Synonym: Ping_0188

Alternate gene names: 119943975

Gene position: 239281-238361 (Counterclockwise)

Preceding gene: 119943976

Following gene: 119943973

Centisome position: 5.25

GC content: 43.32

Gene sequence:

>921_bases
ATGAGCTCTTACAAACTACAACTCAATTTCTGCCATCCTAAATACTGGCTAACCTGGTTGGGCATTTTACTGCTTTTTTT
AAGCTCTTTTTTACCCTATGGTCATTTACTTGCTATCGGTAAATTTCTGGGCCAGATTACAGCCCGTATGGCCAAATCCA
GATATAAAATTGCCTACCGGAATATTGAGTTATGTTTTCCCAACTTAAGTCCGAGTGAAATCACTACAAAAGTGCAGACA
AATTTTGAAAATACCGGTATTGCGATCTTAGAGATAGGGATGGCATGGTTTTGGCCGAGTTGGCGGGTGCGGGGATTATT
AAAAGTGAGCGGCCTTGAGCATATTGAACAAGCCAGAATGCACAATCAACCCATTATTTTTTTGGGGGCGCATTTTCTCC
CTCTAGAAATGACCGGACGCGCTACGGGCTTGCAACTACCTTCTGCCGCAGTTTACCGCAAACACCGTAACCCCGTCTTA
GATTCCTGGTTTTACTGGGGCAGAATGCGTGAAAATAAAGCGATGCTAGATCGTTCCGACTTTAAAGCCATGTTACGCAC
CTTAAAAGGGGGCACACCCCTATGGTATGCGCCGGATCACGATTATGGACGCCATAAAAGCAGTGTTTTTGCACCTTTTT
TTACAGTGCAGAATACTAATACAATATCAGGTACCAGCACGCTCGCACGATTAAGCAAGGGCTGTGTGATCCCGGCATTC
TTAAAACGCCTTCCTGGTTTGCAGGGGTATGAATTAGTCTTTTATCCAAAGATAGAAAATTTTCCCAGTAAAGATCTGGT
GGCCGATGCACAAAAAACCAACCAGCAGATTGAAAACATGATTTTAGAATGTGATGAGCAATATATGTGGGTTCATCGTC
GTTTTAAAACACGGCCGGAAGGCGAAGACTCTTTCTATTAA

Upstream 100 bases:

>100_bases
GACCTTATAAGAAAAGGCATTATAGAAAAAAGACGGTAAAATATGCATTTAGCAGAGAATTAGGTAGAATAATTTTATTA
TTTTTTACGGTGAATCTCCT

Downstream 100 bases:

>100_bases
AGAGGAGTCATTTACTTTTGAAAATAGATCACAGCACAGTAACTGTACCCAAATTATTTGAATATGCAGATTTAGCATCT
TCAAATAATTTGGGTAATTT

Product: lipid A biosynthesis lauroyl (or palmitoleoyl) acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MSSYKLQLNFCHPKYWLTWLGILLLFLSSFLPYGHLLAIGKFLGQITARMAKSRYKIAYRNIELCFPNLSPSEITTKVQT
NFENTGIAILEIGMAWFWPSWRVRGLLKVSGLEHIEQARMHNQPIIFLGAHFLPLEMTGRATGLQLPSAAVYRKHRNPVL
DSWFYWGRMRENKAMLDRSDFKAMLRTLKGGTPLWYAPDHDYGRHKSSVFAPFFTVQNTNTISGTSTLARLSKGCVIPAF
LKRLPGLQGYELVFYPKIENFPSKDLVADAQKTNQQIENMILECDEQYMWVHRRFKTRPEGEDSFY

Sequences:

>Translated_306_residues
MSSYKLQLNFCHPKYWLTWLGILLLFLSSFLPYGHLLAIGKFLGQITARMAKSRYKIAYRNIELCFPNLSPSEITTKVQT
NFENTGIAILEIGMAWFWPSWRVRGLLKVSGLEHIEQARMHNQPIIFLGAHFLPLEMTGRATGLQLPSAAVYRKHRNPVL
DSWFYWGRMRENKAMLDRSDFKAMLRTLKGGTPLWYAPDHDYGRHKSSVFAPFFTVQNTNTISGTSTLARLSKGCVIPAF
LKRLPGLQGYELVFYPKIENFPSKDLVADAQKTNQQIENMILECDEQYMWVHRRFKTRPEGEDSFY
>Mature_305_residues
SSYKLQLNFCHPKYWLTWLGILLLFLSSFLPYGHLLAIGKFLGQITARMAKSRYKIAYRNIELCFPNLSPSEITTKVQTN
FENTGIAILEIGMAWFWPSWRVRGLLKVSGLEHIEQARMHNQPIIFLGAHFLPLEMTGRATGLQLPSAAVYRKHRNPVLD
SWFYWGRMRENKAMLDRSDFKAMLRTLKGGTPLWYAPDHDYGRHKSSVFAPFFTVQNTNTISGTSTLARLSKGCVIPAFL
KRLPGLQGYELVFYPKIENFPSKDLVADAQKTNQQIENMILECDEQYMWVHRRFKTRPEGEDSFY

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=302, Percent_Identity=46.0264900662252, Blast_Score=285, Evalue=3e-78,
Organism=Escherichia coli, GI87082094, Length=302, Percent_Identity=43.3774834437086, Blast_Score=263, Evalue=1e-71,
Organism=Escherichia coli, GI1788161, Length=300, Percent_Identity=26.3333333333333, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 35443; Mature: 35311

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSYKLQLNFCHPKYWLTWLGILLLFLSSFLPYGHLLAIGKFLGQITARMAKSRYKIAYR
CCCEEEEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEEEE
NIELCFPNLSPSEITTKVQTNFENTGIAILEIGMAWFWPSWRVRGLLKVSGLEHIEQARM
EEEEEECCCCHHHHEEHHCCCCCCCCEEEEEECHHHCCCCHHHHHHHHHHHHHHHHHHHH
HNQPIIFLGAHFLPLEMTGRATGLQLPSAAVYRKHRNPVLDSWFYWGRMRENKAMLDRSD
CCCCEEEEECCEEEEEECCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCHHHHCHHH
FKAMLRTLKGGTPLWYAPDHDYGRHKSSVFAPFFTVQNTNTISGTSTLARLSKGCVIPAF
HHHHHHHHCCCCCEEECCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHCCCCHHHH
LKRLPGLQGYELVFYPKIENFPSKDLVADAQKTNQQIENMILECDEQYMWVHRRFKTRPE
HHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
GEDSFY
CCCCCC
>Mature Secondary Structure 
SSYKLQLNFCHPKYWLTWLGILLLFLSSFLPYGHLLAIGKFLGQITARMAKSRYKIAYR
CCEEEEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEEEE
NIELCFPNLSPSEITTKVQTNFENTGIAILEIGMAWFWPSWRVRGLLKVSGLEHIEQARM
EEEEEECCCCHHHHEEHHCCCCCCCCEEEEEECHHHCCCCHHHHHHHHHHHHHHHHHHHH
HNQPIIFLGAHFLPLEMTGRATGLQLPSAAVYRKHRNPVLDSWFYWGRMRENKAMLDRSD
CCCCEEEEECCEEEEEECCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCHHHHCHHH
FKAMLRTLKGGTPLWYAPDHDYGRHKSSVFAPFFTVQNTNTISGTSTLARLSKGCVIPAF
HHHHHHHHCCCCCEEECCCCCCCCCCCCEEEEEEEECCCCCCCHHHHHHHHHCCCCHHHH
LKRLPGLQGYELVFYPKIENFPSKDLVADAQKTNQQIENMILECDEQYMWVHRRFKTRPE
HHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCC
GEDSFY
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]