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The map label for this gene is menE [H]

Identifier: 119943976

GI number: 119943976

Start: 239372

End: 240769

Strand: Reverse

Name: menE [H]

Synonym: Ping_0189

Alternate gene names: 119943976

Gene position: 240769-239372 (Counterclockwise)

Preceding gene: 119943977

Following gene: 119943975

Centisome position: 5.28

GC content: 41.49

Gene sequence:

>1398_bases
ATGGACAAGCAACTGAGTTTTTGCCCTGTCCGTCATCAGGCGATTATCAATGCGAATGCGCTAGCCATCGAGTCTGACGG
GGTCTCGCTCACCTACCAGCAGTTAGACAATAGACTCAACATATTAAGCAGACAGCTGCTGAATCTACATTTAAAAGCGG
GAGACAGGTTAGTCTGTATCTCGGAAAATTCTCTTAAGGTGATTTTACTGCAGCTCACCTGCATACGCTTAGGGCTTATT
TTCTGTCCTTTAAATCCGCGTTTTAGTGCGGCTGAAATCGAGCAACGTTTAAACGTTCTGAAAAGTGACTTTATTTGGCT
TGCCAATCCGCAAAAACATTCGCATTTAAGCAGCCTTGCAATAGACTTTTCAATTTACCCTAAAGGGGTAGTGGCTCCTG
CACCGCTAAAAATAGATCCCCAAAAAGTCTGTAATATCATTTTCACATCCGGTAGTAGTGGTCAGCCAAAGGCTGTTATG
CACTGTTTTAGCAATCATTATTATAGTGCTATTGGTTCACAATACGGTATTGCATTACGGGCAAAAGATAGAAATCTCTT
GTCATTGCCCCTGTTCCATATCAGTGGATTCGCAACGGTTATCCGTACATTGATAGCCGGAGCCACCTTGGTTGTTAGCG
CTGAAAAACTCTGTGTTAGTTTATTAAAAAAGGAGAAAATAACCCACCTTTCTTTGGTTGCCACACAGCTTTATCGTTTA
TTAAGCAGCTTAGATTTTCAGCAAAAGGAACTCTCAATTAAACACCTTCTTCTGGGTGGCAGTCTTTTTTCTGACCGGTT
GTTGGTGCAAACCAGGCAACGGGGGTTTGTGTATCATTTAAGTTATGGTTTAAGTGAGATGAGCTCACAGGTTGCCACCT
CCACCAACAGTAAAAAATTGATCCTGTTACCCGGTCAGCAGGTAAAAATAATAAATAATGAGATCTGCTTACGTGGAAAA
ATGCGCTTTGCAGGCTATTTTAATGGTCATTTAGACTCCAGTCGTTTGCCTGAAGATCAATGGTTTGCCAGTAAAGATTT
AGGCGCATTAATGGGCAATGAGTTATGTATTTTAGGTCGTACAGACAGGTTAATTATCTCCGGTGGCGAAAATATTCAGG
CTGAAGAACTGGAGTCAATACTGTTAAACTTTGCACATATTAAACAGGCTTATATTGTTGCTGTAACGGATCCTGTTTTT
GGAGAGCGCCCGGCAGCTTTTATAGATTGGGAAAATGGTAAGATTAAACAACAACAACTACAGATTTACCTGCAGGATAA
ACTGAGCAGTTATAAGCAGCCAGTACATTATTTTACTCTGCCTGAGCAAACAGGCCTTAAAGTGTCATTAAGAGAGCTGC
AGGTAATTGCTCAGGTCGCCCACAACATGACCTTATAA

Upstream 100 bases:

>100_bases
CGCTAAAATTTTTTGAAAGTGCCATCTTAAATAAAAATTCAGATAAAAAGTTAGATAATAAAACACCTATTAATCAACAA
GTTAAACATTTGGAGTGTCT

Downstream 100 bases:

>100_bases
GAAAAGGCATTATAGAAAAAAGACGGTAAAATATGCATTTAGCAGAGAATTAGGTAGAATAATTTTATTATTTTTTACGG
TGAATCTCCTATGAGCTCTT

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MDKQLSFCPVRHQAIINANALAIESDGVSLTYQQLDNRLNILSRQLLNLHLKAGDRLVCISENSLKVILLQLTCIRLGLI
FCPLNPRFSAAEIEQRLNVLKSDFIWLANPQKHSHLSSLAIDFSIYPKGVVAPAPLKIDPQKVCNIIFTSGSSGQPKAVM
HCFSNHYYSAIGSQYGIALRAKDRNLLSLPLFHISGFATVIRTLIAGATLVVSAEKLCVSLLKKEKITHLSLVATQLYRL
LSSLDFQQKELSIKHLLLGGSLFSDRLLVQTRQRGFVYHLSYGLSEMSSQVATSTNSKKLILLPGQQVKIINNEICLRGK
MRFAGYFNGHLDSSRLPEDQWFASKDLGALMGNELCILGRTDRLIISGGENIQAEELESILLNFAHIKQAYIVAVTDPVF
GERPAAFIDWENGKIKQQQLQIYLQDKLSSYKQPVHYFTLPEQTGLKVSLRELQVIAQVAHNMTL

Sequences:

>Translated_465_residues
MDKQLSFCPVRHQAIINANALAIESDGVSLTYQQLDNRLNILSRQLLNLHLKAGDRLVCISENSLKVILLQLTCIRLGLI
FCPLNPRFSAAEIEQRLNVLKSDFIWLANPQKHSHLSSLAIDFSIYPKGVVAPAPLKIDPQKVCNIIFTSGSSGQPKAVM
HCFSNHYYSAIGSQYGIALRAKDRNLLSLPLFHISGFATVIRTLIAGATLVVSAEKLCVSLLKKEKITHLSLVATQLYRL
LSSLDFQQKELSIKHLLLGGSLFSDRLLVQTRQRGFVYHLSYGLSEMSSQVATSTNSKKLILLPGQQVKIINNEICLRGK
MRFAGYFNGHLDSSRLPEDQWFASKDLGALMGNELCILGRTDRLIISGGENIQAEELESILLNFAHIKQAYIVAVTDPVF
GERPAAFIDWENGKIKQQQLQIYLQDKLSSYKQPVHYFTLPEQTGLKVSLRELQVIAQVAHNMTL
>Mature_465_residues
MDKQLSFCPVRHQAIINANALAIESDGVSLTYQQLDNRLNILSRQLLNLHLKAGDRLVCISENSLKVILLQLTCIRLGLI
FCPLNPRFSAAEIEQRLNVLKSDFIWLANPQKHSHLSSLAIDFSIYPKGVVAPAPLKIDPQKVCNIIFTSGSSGQPKAVM
HCFSNHYYSAIGSQYGIALRAKDRNLLSLPLFHISGFATVIRTLIAGATLVVSAEKLCVSLLKKEKITHLSLVATQLYRL
LSSLDFQQKELSIKHLLLGGSLFSDRLLVQTRQRGFVYHLSYGLSEMSSQVATSTNSKKLILLPGQQVKIINNEICLRGK
MRFAGYFNGHLDSSRLPEDQWFASKDLGALMGNELCILGRTDRLIISGGENIQAEELESILLNFAHIKQAYIVAVTDPVF
GERPAAFIDWENGKIKQQQLQIYLQDKLSSYKQPVHYFTLPEQTGLKVSLRELQVIAQVAHNMTL

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=505, Percent_Identity=24.1584158415842, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1788595, Length=462, Percent_Identity=32.034632034632, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI145693145, Length=483, Percent_Identity=24.223602484472, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1788107, Length=349, Percent_Identity=23.7822349570201, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1786810, Length=318, Percent_Identity=23.5849056603774, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17558820, Length=457, Percent_Identity=25.164113785558, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI32563687, Length=441, Percent_Identity=23.5827664399093, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17560308, Length=493, Percent_Identity=23.7322515212982, Blast_Score=102, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI71994703, Length=504, Percent_Identity=21.6269841269841, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71994694, Length=504, Percent_Identity=21.6269841269841, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71994690, Length=504, Percent_Identity=21.6269841269841, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17559526, Length=326, Percent_Identity=24.2331288343558, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71996755, Length=452, Percent_Identity=22.3451327433628, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17557194, Length=492, Percent_Identity=20.3252032520325, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24581924, Length=515, Percent_Identity=24.8543689320388, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21356947, Length=451, Percent_Identity=22.6164079822616, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21355181, Length=476, Percent_Identity=23.5294117647059, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21358303, Length=448, Percent_Identity=22.0982142857143, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24648253, Length=479, Percent_Identity=20.8768267223382, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI20130357, Length=459, Percent_Identity=21.7864923747277, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 51935; Mature: 51935

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKQLSFCPVRHQAIINANALAIESDGVSLTYQQLDNRLNILSRQLLNLHLKAGDRLVCI
CCCCCCCCCCCHHEEECCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHEEECCCCEEEEE
SENSLKVILLQLTCIRLGLIFCPLNPRFSAAEIEQRLNVLKSDFIWLANPQKHSHLSSLA
ECCCCEEEHHHHHHHHHCEEEECCCCCCCHHHHHHHHHHHHHCCEEEECCCHHHHHHHEE
IDFSIYPKGVVAPAPLKIDPQKVCNIIFTSGSSGQPKAVMHCFSNHYYSAIGSQYGIALR
EEEEECCCCCCCCCCCCCCHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEE
AKDRNLLSLPLFHISGFATVIRTLIAGATLVVSAEKLCVSLLKKEKITHLSLVATQLYRL
ECCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSSLDFQQKELSIKHLLLGGSLFSDRLLVQTRQRGFVYHLSYGLSEMSSQVATSTNSKKL
HHHCCCCHHHHHHHHEEECCCHHCCHHHEEHHCCCEEEEEECCHHHHHHHHHCCCCCCEE
ILLPGQQVKIINNEICLRGKMRFAGYFNGHLDSSRLPEDQWFASKDLGALMGNELCILGR
EEECCCEEEEECCCEEEEECEEEEEEECCCCCCCCCCCHHHCCCCCHHHHHCCCEEEEEC
TDRLIISGGENIQAEELESILLNFAHIKQAYIVAVTDPVFGERPAAFIDWENGKIKQQQL
CCEEEEECCCCCCHHHHHHHHHHHHHHCEEEEEEEECCCCCCCCCEEEECCCCCEEHHHH
QIYLQDKLSSYKQPVHYFTLPEQTGLKVSLRELQVIAQVAHNMTL
HHHHHHHHHHHCCCCEEEECCCCCCCEEHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDKQLSFCPVRHQAIINANALAIESDGVSLTYQQLDNRLNILSRQLLNLHLKAGDRLVCI
CCCCCCCCCCCHHEEECCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHEEECCCCEEEEE
SENSLKVILLQLTCIRLGLIFCPLNPRFSAAEIEQRLNVLKSDFIWLANPQKHSHLSSLA
ECCCCEEEHHHHHHHHHCEEEECCCCCCCHHHHHHHHHHHHHCCEEEECCCHHHHHHHEE
IDFSIYPKGVVAPAPLKIDPQKVCNIIFTSGSSGQPKAVMHCFSNHYYSAIGSQYGIALR
EEEEECCCCCCCCCCCCCCHHHHEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEE
AKDRNLLSLPLFHISGFATVIRTLIAGATLVVSAEKLCVSLLKKEKITHLSLVATQLYRL
ECCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSSLDFQQKELSIKHLLLGGSLFSDRLLVQTRQRGFVYHLSYGLSEMSSQVATSTNSKKL
HHHCCCCHHHHHHHHEEECCCHHCCHHHEEHHCCCEEEEEECCHHHHHHHHHCCCCCCEE
ILLPGQQVKIINNEICLRGKMRFAGYFNGHLDSSRLPEDQWFASKDLGALMGNELCILGR
EEECCCEEEEECCCEEEEECEEEEEEECCCCCCCCCCCHHHCCCCCHHHHHCCCEEEEEC
TDRLIISGGENIQAEELESILLNFAHIKQAYIVAVTDPVFGERPAAFIDWENGKIKQQQL
CCEEEEECCCCCCHHHHHHHHHHHHHHCEEEEEEEECCCCCCCCCEEEECCCCCEEHHHH
QIYLQDKLSSYKQPVHYFTLPEQTGLKVSLRELQVIAQVAHNMTL
HHHHHHHHHHHCCCCEEEECCCCCCCEEHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 8206837 [H]