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The map label for this gene is epsE [H]

Identifier: 119943891

GI number: 119943891

Start: 131375

End: 132910

Strand: Direct

Name: epsE [H]

Synonym: Ping_0098

Alternate gene names: 119943891

Gene position: 131375-132910 (Clockwise)

Preceding gene: 119943890

Following gene: 119943892

Centisome position: 2.88

GC content: 42.19

Gene sequence:

>1536_bases
ATGATCACGCCTGAGCAAGATACTTTGCAGGTGGTGGCGGCGCTCAATGAAGCTGAGCTTATTGAAGTCACGCCGGGTGG
TCAGTTACCTTTCCCATTTTCAAAAAAACATGAGTTAGTGATTTTTTACGAAAATGAGAATGCACAGCTTTGTTACAAGA
AAGCCCCGAGTGCTGATTTGATACTTGAAGTGCGTCGTGCGATCGGTTGTGAATTTACTTTAAAACAGGTAGATAATGTC
CTTTTCGAGCAGTTAGTAACAACGGCTTATCAAACTTCTTCTTCTCAAGCACAACAATTGATGGAAGATATTGGCAGTGA
TGATCTTTTTACTCTGGTAGATGAATTGCCTCTGGATGAGGATTTATTAGAAACACAAGATGATGCCCCGATTATTAAAT
TAATTAATGCTATGTTGGGTGAGGCTATTAAAGAAGAAGCTTCCGATATTCATATCGAAACCTTTGAAAATAGTTTAGTT
ATTCGCTTCCGTATTGATGGTTTATTACGAGAAATTCTTAAACCACAACGTAAGTTGTCTAACTTATTGGTTTCTCGTAT
TAAAGTAATGGCGAAGTTAGATATTGCTGAAAAGCGGGTGCCACAGGATGGCCGTATTTCCGTACGTATTGGTGGTCGGG
CGGTTGATGTGCGTGTTTCAACTATGCCTTCAAGTCATGGTGAGCGAGTTGTACTGCGTTTATTGGATAAGAATGTCATT
AAATTAGACCTTGCAACCTTAGGTATGACAGAAGAAAATCACCAACTGGTGCGCGACCTTATTCATAAACCCCATGGCAT
TATTTTGGTCACAGGACCAACAGGTTCAGGTAAAAGTACGACCTTGTATGCCAGTTTATTAGAGATAAACTCGAAAGATC
GTAATATCCTCACGGTTGAAGATCCTATTGAGTATGCCATTGATGGTGTCGGACAAACTCAGGTAAATACCAAAGTGGAC
ATGACTTTTGCGCGGGGATTACGGGCAATTTTACGTCAGGATCCCGATGTTGTTATGATTGGTGAGATTCGTGATTTAGA
AACCGCGCAGATTGCCGTACAAGCCAGTTTAACCGGACATCTGGTTTTATCAACCCTGCACACTAACAGTGCTGTGGGTG
CGGTGACTCGTTTGCATGATATGGGAGTTGAGCCGTTTCTGTTATCTTCCAGTTTATTAGGGGTTTTAGCGCAACGATTA
GTCAGGCGTTTATGCCCTGAATGTAGACAAACGCACACCACAACAGCACAAGAAAAGCAGATTTTAGGTTTGCAGGAAAA
AGATAATGCGCGTATCTATAAAGCCAAAGGTTGCTCTCAATGCAATCAAACCGGCTATAGAGGGCGTACCGGTATCCATG
AGTTATTAATCGTTGATGAGCAGGTTCGCGAATTAATTCATTCCGGAGCAGGAGAGCAGGCGGTCGAAAAATTAATTCGT
CAGCGTACGCCCAGTATTCGCGATGATGGTCTAGCTAAAGTGTTAGCAGGAGTAACCACATTGGAAGAGGTTCTACGTGT
AAGCAGAGAGGATTAA

Upstream 100 bases:

>100_bases
CCATCCTGCCTGAATGGGGGCAAAGTTATGAAATTAATCCGGATCATTTCATCAAGTCAGAAAGTAAAATCAGAAAACCA
ACAACAGAAGGTGTGAACTA

Downstream 100 bases:

>100_bases
CGTGGCAACATTTTCTTATAAAGCAATGGATGATCGAGGAAAACTGAAAAAAGGTACTGAAGAAGCTGATTCGGCACGCT
TGTTGCGGCACCAGTTACGC

Product: general secretory pathway protein E

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MITPEQDTLQVVAALNEAELIEVTPGGQLPFPFSKKHELVIFYENENAQLCYKKAPSADLILEVRRAIGCEFTLKQVDNV
LFEQLVTTAYQTSSSQAQQLMEDIGSDDLFTLVDELPLDEDLLETQDDAPIIKLINAMLGEAIKEEASDIHIETFENSLV
IRFRIDGLLREILKPQRKLSNLLVSRIKVMAKLDIAEKRVPQDGRISVRIGGRAVDVRVSTMPSSHGERVVLRLLDKNVI
KLDLATLGMTEENHQLVRDLIHKPHGIILVTGPTGSGKSTTLYASLLEINSKDRNILTVEDPIEYAIDGVGQTQVNTKVD
MTFARGLRAILRQDPDVVMIGEIRDLETAQIAVQASLTGHLVLSTLHTNSAVGAVTRLHDMGVEPFLLSSSLLGVLAQRL
VRRLCPECRQTHTTTAQEKQILGLQEKDNARIYKAKGCSQCNQTGYRGRTGIHELLIVDEQVRELIHSGAGEQAVEKLIR
QRTPSIRDDGLAKVLAGVTTLEEVLRVSRED

Sequences:

>Translated_511_residues
MITPEQDTLQVVAALNEAELIEVTPGGQLPFPFSKKHELVIFYENENAQLCYKKAPSADLILEVRRAIGCEFTLKQVDNV
LFEQLVTTAYQTSSSQAQQLMEDIGSDDLFTLVDELPLDEDLLETQDDAPIIKLINAMLGEAIKEEASDIHIETFENSLV
IRFRIDGLLREILKPQRKLSNLLVSRIKVMAKLDIAEKRVPQDGRISVRIGGRAVDVRVSTMPSSHGERVVLRLLDKNVI
KLDLATLGMTEENHQLVRDLIHKPHGIILVTGPTGSGKSTTLYASLLEINSKDRNILTVEDPIEYAIDGVGQTQVNTKVD
MTFARGLRAILRQDPDVVMIGEIRDLETAQIAVQASLTGHLVLSTLHTNSAVGAVTRLHDMGVEPFLLSSSLLGVLAQRL
VRRLCPECRQTHTTTAQEKQILGLQEKDNARIYKAKGCSQCNQTGYRGRTGIHELLIVDEQVRELIHSGAGEQAVEKLIR
QRTPSIRDDGLAKVLAGVTTLEEVLRVSRED
>Mature_511_residues
MITPEQDTLQVVAALNEAELIEVTPGGQLPFPFSKKHELVIFYENENAQLCYKKAPSADLILEVRRAIGCEFTLKQVDNV
LFEQLVTTAYQTSSSQAQQLMEDIGSDDLFTLVDELPLDEDLLETQDDAPIIKLINAMLGEAIKEEASDIHIETFENSLV
IRFRIDGLLREILKPQRKLSNLLVSRIKVMAKLDIAEKRVPQDGRISVRIGGRAVDVRVSTMPSSHGERVVLRLLDKNVI
KLDLATLGMTEENHQLVRDLIHKPHGIILVTGPTGSGKSTTLYASLLEINSKDRNILTVEDPIEYAIDGVGQTQVNTKVD
MTFARGLRAILRQDPDVVMIGEIRDLETAQIAVQASLTGHLVLSTLHTNSAVGAVTRLHDMGVEPFLLSSSLLGVLAQRL
VRRLCPECRQTHTTTAQEKQILGLQEKDNARIYKAKGCSQCNQTGYRGRTGIHELLIVDEQVRELIHSGAGEQAVEKLIR
QRTPSIRDDGLAKVLAGVTTLEEVLRVSRED

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=480, Percent_Identity=57.5, Blast_Score=557, Evalue=1e-160,
Organism=Escherichia coli, GI1786296, Length=384, Percent_Identity=44.53125, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI87082188, Length=195, Percent_Identity=43.5897435897436, Blast_Score=124, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 56671; Mature: 56671

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITPEQDTLQVVAALNEAELIEVTPGGQLPFPFSKKHELVIFYENENAQLCYKKAPSADL
CCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCHHH
ILEVRRAIGCEFTLKQVDNVLFEQLVTTAYQTSSSQAQQLMEDIGSDDLFTLVDELPLDE
HHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHCCCCH
DLLETQDDAPIIKLINAMLGEAIKEEASDIHIETFENSLVIRFRIDGLLREILKPQRKLS
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEEEEHHHHHHHHHHHHHHHH
NLLVSRIKVMAKLDIAEKRVPQDGRISVRIGGRAVDVRVSTMPSSHGERVVLRLLDKNVI
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEEEEECCCCCCHHHHHHHHCCCCE
KLDLATLGMTEENHQLVRDLIHKPHGIILVTGPTGSGKSTTLYASLLEINSKDRNILTVE
EEEHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEC
DPIEYAIDGVGQTQVNTKVDMTFARGLRAILRQDPDVVMIGEIRDLETAQIAVQASLTGH
CCHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEECCCCE
LVLSTLHTNSAVGAVTRLHDMGVEPFLLSSSLLGVLAQRLVRRLCPECRQTHTTTAQEKQ
EEEEEHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCHHHHH
ILGLQEKDNARIYKAKGCSQCNQTGYRGRTGIHELLIVDEQVRELIHSGAGEQAVEKLIR
HCCCCCCCCCEEEECCCCCHHHCCCCCCCCCHHHHHEECHHHHHHHHCCCCHHHHHHHHH
QRTPSIRDDGLAKVLAGVTTLEEVLRVSRED
HCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MITPEQDTLQVVAALNEAELIEVTPGGQLPFPFSKKHELVIFYENENAQLCYKKAPSADL
CCCCCHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCCEEEEECCCCHHH
ILEVRRAIGCEFTLKQVDNVLFEQLVTTAYQTSSSQAQQLMEDIGSDDLFTLVDELPLDE
HHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHCCCCH
DLLETQDDAPIIKLINAMLGEAIKEEASDIHIETFENSLVIRFRIDGLLREILKPQRKLS
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCEEEEEEHHHHHHHHHHHHHHHH
NLLVSRIKVMAKLDIAEKRVPQDGRISVRIGGRAVDVRVSTMPSSHGERVVLRLLDKNVI
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCEEEEEEEEECCCCCCHHHHHHHHCCCCE
KLDLATLGMTEENHQLVRDLIHKPHGIILVTGPTGSGKSTTLYASLLEINSKDRNILTVE
EEEHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEEC
DPIEYAIDGVGQTQVNTKVDMTFARGLRAILRQDPDVVMIGEIRDLETAQIAVQASLTGH
CCHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEECCCCE
LVLSTLHTNSAVGAVTRLHDMGVEPFLLSSSLLGVLAQRLVRRLCPECRQTHTTTAQEKQ
EEEEEHHCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCHHHHH
ILGLQEKDNARIYKAKGCSQCNQTGYRGRTGIHELLIVDEQVRELIHSGAGEQAVEKLIR
HCCCCCCCCCEEEECCCCCHHHCCCCCCCCCHHHHHEECHHHHHHHHCCCCHHHHHHHHH
QRTPSIRDDGLAKVLAGVTTLEEVLRVSRED
HCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]