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The map label for this gene is pckA [H]

Identifier: 119943886

GI number: 119943886

Start: 124919

End: 126550

Strand: Reverse

Name: pckA [H]

Synonym: Ping_0093

Alternate gene names: 119943886

Gene position: 126550-124919 (Counterclockwise)

Preceding gene: 119943887

Following gene: 119943884

Centisome position: 2.78

GC content: 41.05

Gene sequence:

>1632_bases
ATGGCTACGACGAACACATTTTCTGCTGAAACATTAAGTCAATACGGTATCACAGACATTCCAGAAGTTATCTACAATCC
GAGTTATGACAAACTCTATGAAGAAGAGATAGCAGAAAATTTAGAGGGATTTGAAAAATGTACCGTCACTTCAAATGGTA
CTATCGCGGTTGATACGGGTATTTTTACCGGCCGCTCCCCAAAAGATAAATATATTGTTAAAGATGATACTACTCGCGAC
ACAGTTTGGTGGAGTGACCAGGGTAAAAATGATAATAAAGCTATCGATCAGGGGACTTGGGATTATTTAAAAAAAATCGT
AGCCAAAGATCTTTCCACTAAACAACTCTTTGTGGTTGACGCTTTTTGCGGAGCAAATGAAAACTCCCGCTTAAAAGTTC
GTTTTATTATGGAAGTTGCCTGGCAGGCACATTTTGTAACCAATATGTTCATCCGCCCTACGCAAGATGAACTTGTTGAT
TTTGAACCCGATTTTGTTGTAATGAATGGCTCTAAAACAACCAATCAAGAGTGGAAATCTCACGGATTAAACTCTGAAAA
CTTTGTTGCTTTTAATCTTACAGAAAAAATTCAATTGATCGGTGGCACCTGGTACGGCGGTGAAATGAAAAAAGGGCTGT
TTTCAGTGATGAACTACCTGTTACCCCTTAATGGCATGGCATCTATGCATTGTTCAGCCAATGTGGGTGAAGCTGGCGAT
ACTGCGATTTTCTTTGGTTTATCAGGTACCGGTAAAACCACCCTGTCAACGGATCCAAAACGTAAATTAATTGGTGATGA
TGAACATGGCTGGGATGATGACGGTGTATTTAACTTTGAAGGTGGTTGTTACGCTAAAACTGTCCGTTTAAGCGAAGAAG
CTGAACCTGAGATTTTTAACGCGATTAGACGCGATGCATTACTTGAAAATGTGGTTGTCGGCGCTGATGGTGTTGTCGAT
TATGATGATGCAAGTAAAACTGAAAATGGACGTGTTTCATACCCTATTTATCATATTGAAAATATTGTAAAACCTGTATC
GAAAGGCGGCCATGCCAAGAAAGTTATTTTCTTAACTGCTGATGCTTTTGGTGTTTTACCCCCAGTTTCCAAACTGACTA
AAGCGCAAGCGCAGTATCACTTCCTGTCGGGCTTTACCGCAAAATTAGCAGGTACTGAGCGTGGTATTACCGAGCCAACA
CCGACCTTCTCTGCTTGTTTTGGAGCTGCATTTTTACTGCTACACCCAACACAATATGCTGAGCAGCTAGTTAAACGTAT
GGAAGCAAGTGGTGCAGAAGCATACCTTGTTAATACAGGTTGGAATGGCTCAGGTAAGCGCATCTCAATCAAAGATACTC
GTGCCATTATTGACTCTATCTTAAATGGATCAATTGAAGACGCACCCACTAAAACAATCCCATACTTTAACCTGGAAGTT
CCATTAGCTTTACCCGGTGCTCGCAGCGACATTCTTGATCCGCGCGATACCTATGCAACGCCTGCCGAGTGGGAAACAAA
AGCAGAAGAATTATCAAATCTGTTTATTAATAACTTTGCCCGTTATACCGATAAAGCAGAAGGGCTAGCCCTTGTTTCTG
CAGGACCACAATTAACCAAACTACTTGTTTAA

Upstream 100 bases:

>100_bases
AAATACATCCTCTAATACCTTACCCTGTAAGTCTCATTTTTATACAATTTAATGGCTAGGCAATATTCATTAAATAAACA
CTACCTGAGGGAAATTGAAC

Downstream 100 bases:

>100_bases
TTTATAAAAAAGGTTGAATCTAAGCCAGTTAAGTTCCCTCTTGACTGGCTTTTTTTTAATTAAAATATCAATCATAGACT
CTTCTACCCATTATTCAACT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 543; Mature: 542

Protein sequence:

>543_residues
MATTNTFSAETLSQYGITDIPEVIYNPSYDKLYEEEIAENLEGFEKCTVTSNGTIAVDTGIFTGRSPKDKYIVKDDTTRD
TVWWSDQGKNDNKAIDQGTWDYLKKIVAKDLSTKQLFVVDAFCGANENSRLKVRFIMEVAWQAHFVTNMFIRPTQDELVD
FEPDFVVMNGSKTTNQEWKSHGLNSENFVAFNLTEKIQLIGGTWYGGEMKKGLFSVMNYLLPLNGMASMHCSANVGEAGD
TAIFFGLSGTGKTTLSTDPKRKLIGDDEHGWDDDGVFNFEGGCYAKTVRLSEEAEPEIFNAIRRDALLENVVVGADGVVD
YDDASKTENGRVSYPIYHIENIVKPVSKGGHAKKVIFLTADAFGVLPPVSKLTKAQAQYHFLSGFTAKLAGTERGITEPT
PTFSACFGAAFLLLHPTQYAEQLVKRMEASGAEAYLVNTGWNGSGKRISIKDTRAIIDSILNGSIEDAPTKTIPYFNLEV
PLALPGARSDILDPRDTYATPAEWETKAEELSNLFINNFARYTDKAEGLALVSAGPQLTKLLV

Sequences:

>Translated_543_residues
MATTNTFSAETLSQYGITDIPEVIYNPSYDKLYEEEIAENLEGFEKCTVTSNGTIAVDTGIFTGRSPKDKYIVKDDTTRD
TVWWSDQGKNDNKAIDQGTWDYLKKIVAKDLSTKQLFVVDAFCGANENSRLKVRFIMEVAWQAHFVTNMFIRPTQDELVD
FEPDFVVMNGSKTTNQEWKSHGLNSENFVAFNLTEKIQLIGGTWYGGEMKKGLFSVMNYLLPLNGMASMHCSANVGEAGD
TAIFFGLSGTGKTTLSTDPKRKLIGDDEHGWDDDGVFNFEGGCYAKTVRLSEEAEPEIFNAIRRDALLENVVVGADGVVD
YDDASKTENGRVSYPIYHIENIVKPVSKGGHAKKVIFLTADAFGVLPPVSKLTKAQAQYHFLSGFTAKLAGTERGITEPT
PTFSACFGAAFLLLHPTQYAEQLVKRMEASGAEAYLVNTGWNGSGKRISIKDTRAIIDSILNGSIEDAPTKTIPYFNLEV
PLALPGARSDILDPRDTYATPAEWETKAEELSNLFINNFARYTDKAEGLALVSAGPQLTKLLV
>Mature_542_residues
ATTNTFSAETLSQYGITDIPEVIYNPSYDKLYEEEIAENLEGFEKCTVTSNGTIAVDTGIFTGRSPKDKYIVKDDTTRDT
VWWSDQGKNDNKAIDQGTWDYLKKIVAKDLSTKQLFVVDAFCGANENSRLKVRFIMEVAWQAHFVTNMFIRPTQDELVDF
EPDFVVMNGSKTTNQEWKSHGLNSENFVAFNLTEKIQLIGGTWYGGEMKKGLFSVMNYLLPLNGMASMHCSANVGEAGDT
AIFFGLSGTGKTTLSTDPKRKLIGDDEHGWDDDGVFNFEGGCYAKTVRLSEEAEPEIFNAIRRDALLENVVVGADGVVDY
DDASKTENGRVSYPIYHIENIVKPVSKGGHAKKVIFLTADAFGVLPPVSKLTKAQAQYHFLSGFTAKLAGTERGITEPTP
TFSACFGAAFLLLHPTQYAEQLVKRMEASGAEAYLVNTGWNGSGKRISIKDTRAIIDSILNGSIEDAPTKTIPYFNLEVP
LALPGARSDILDPRDTYATPAEWETKAEELSNLFINNFARYTDKAEGLALVSAGPQLTKLLV

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=540, Percent_Identity=73.8888888888889, Blast_Score=859, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322950, Length=518, Percent_Identity=47.2972972972973, Blast_Score=464, Evalue=1e-131,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 59849; Mature: 59718

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATTNTFSAETLSQYGITDIPEVIYNPSYDKLYEEEIAENLEGFEKCTVTSNGTIAVDTG
CCCCCCCCHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHEEEEECCCEEEEECC
IFTGRSPKDKYIVKDDTTRDTVWWSDQGKNDNKAIDQGTWDYLKKIVAKDLSTKQLFVVD
EECCCCCCCCEEECCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEE
AFCGANENSRLKVRFIMEVAWQAHFVTNMFIRPTQDELVDFEPDFVVMNGSKTTNQEWKS
EECCCCCCCEEEEEEEEHHHHHHHHHHHEEECCCHHHHCCCCCCEEEECCCCCCCHHHHH
HGLNSENFVAFNLTEKIQLIGGTWYGGEMKKGLFSVMNYLLPLNGMASMHCSANVGEAGD
CCCCCCCEEEEEECCEEEEEECEEECCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCC
TAIFFGLSGTGKTTLSTDPKRKLIGDDEHGWDDDGVFNFEGGCYAKTVRLSEEAEPEIFN
EEEEEEECCCCCCCCCCCCCHHCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCHHHHH
AIRRDALLENVVVGADGVVDYDDASKTENGRVSYPIYHIENIVKPVSKGGHAKKVIFLTA
HHHHHHHHHHHEECCCCEEECCCCCCCCCCEEECCHHHHHHHHHHHHCCCCCEEEEEEEC
DAFGVLPPVSKLTKAQAQYHFLSGFTAKLAGTERGITEPTPTFSACFGAAFLLLHPTQYA
CCCCCCCCHHHHHHHHHHHHHHCCCHHEECCCCCCCCCCCCHHHHHHHHHHHEECCHHHH
EQLVKRMEASGAEAYLVNTGWNGSGKRISIKDTRAIIDSILNGSIEDAPTKTIPYFNLEV
HHHHHHHHCCCCCEEEEECCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
PLALPGARSDILDPRDTYATPAEWETKAEELSNLFINNFARYTDKAEGLALVSAGPQLTK
CEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHH
LLV
HCC
>Mature Secondary Structure 
ATTNTFSAETLSQYGITDIPEVIYNPSYDKLYEEEIAENLEGFEKCTVTSNGTIAVDTG
CCCCCCCHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHEEEEECCCEEEEECC
IFTGRSPKDKYIVKDDTTRDTVWWSDQGKNDNKAIDQGTWDYLKKIVAKDLSTKQLFVVD
EECCCCCCCCEEECCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEE
AFCGANENSRLKVRFIMEVAWQAHFVTNMFIRPTQDELVDFEPDFVVMNGSKTTNQEWKS
EECCCCCCCEEEEEEEEHHHHHHHHHHHEEECCCHHHHCCCCCCEEEECCCCCCCHHHHH
HGLNSENFVAFNLTEKIQLIGGTWYGGEMKKGLFSVMNYLLPLNGMASMHCSANVGEAGD
CCCCCCCEEEEEECCEEEEEECEEECCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCC
TAIFFGLSGTGKTTLSTDPKRKLIGDDEHGWDDDGVFNFEGGCYAKTVRLSEEAEPEIFN
EEEEEEECCCCCCCCCCCCCHHCCCCCCCCCCCCCEEEECCCCEEEEEECCCCCCHHHHH
AIRRDALLENVVVGADGVVDYDDASKTENGRVSYPIYHIENIVKPVSKGGHAKKVIFLTA
HHHHHHHHHHHEECCCCEEECCCCCCCCCCEEECCHHHHHHHHHHHHCCCCCEEEEEEEC
DAFGVLPPVSKLTKAQAQYHFLSGFTAKLAGTERGITEPTPTFSACFGAAFLLLHPTQYA
CCCCCCCCHHHHHHHHHHHHHHCCCHHEECCCCCCCCCCCCHHHHHHHHHHHEECCHHHH
EQLVKRMEASGAEAYLVNTGWNGSGKRISIKDTRAIIDSILNGSIEDAPTKTIPYFNLEV
HHHHHHHHCCCCCEEEEECCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
PLALPGARSDILDPRDTYATPAEWETKAEELSNLFINNFARYTDKAEGLALVSAGPQLTK
CEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHH
LLV
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA