Click here to switch to the map view.

The map label for this gene is ldhA [H]

Identifier: 119943884

GI number: 119943884

Start: 122411

End: 123406

Strand: Reverse

Name: ldhA [H]

Synonym: Ping_0091

Alternate gene names: 119943884

Gene position: 123406-122411 (Counterclockwise)

Preceding gene: 119943886

Following gene: 119943880

Centisome position: 2.71

GC content: 40.76

Gene sequence:

>996_bases
ATGAAAGTTGCCATGTTCAGTACCCGCAATTATGATATTAAACATTTCGATCCACTATTAGCTGTTCAGCAGGAATCAAT
ACAGATAAAGTATTTTGATACCCGCTTAGATATTCATACCAGCCAACTGGCTAAGGGTTATGATGCGGTCTGTGCATTCG
TGAACGATGATCTCGCAGGTCCTGTATTAACTGAACTCGCAAGCCTAGGCATTAAGCTTATCGTGATGCGCTGTGCCGGT
TTTAACAATATAAATTTAGCGGTTAGCAAAAAATTAGGCCTGACTATCATGCGCGTGCCGGCATACTCACCGGAAGCCGT
TGCCGAACACGCTATTGCATTAATGATGACCTTAAACAGACGTATTCATAAAGCTTATCAACGCACCCGTGATGCCAATT
TTTCACTCGATGGATTAGTTGGGTTTAATATGTTTGGTAAAACCATGGGTGTTATCGGCACAGGTAAAATTGGTCTTGCA
ACCATGCGTATTCTAAAGGGATTTGGCTGTGAGATTTTAGCTTATGATCCCTACCCATCTGCACAGGCAATTGAACTTGG
CGCTAACTATGTTTCATTAACAGAGTTATTTACACGAAGTGATGCTATCACCCTGCATTGCCCGTTAACTGACGATAATT
ACCACCTGCTTAATAGAGAATCTTTTGCGCAAATGAAAGAGGGCGTGATGATCATTAATACCAGCCGTGGCGCTTTAATA
AATGCCAAAGATTCAATCGATGCGCTGAAAAGTAAAAAAATAGGCGCATTAGGCTTGGATGTTTACGAAGAGGAAGACTC
TCTTTTTTTTGACGATCACTCCAATGAAATTATCACCGATGATGTGTTTCGCCGTTTATCTTCGTGTCACAATGTTATCT
TTACAGGTCATCAAGCATTTTTAACCCGAGAAGCCTTAACCAGTATTGCCCAAACAACCCTGGATAACTTAAACTGTTTT
GCTTCAAGTAAACCTAATGGTAATGAAGTGAGTTAA

Upstream 100 bases:

>100_bases
TCTGTTATATAGCCCCTTTAATAATTAATTTTTTCTCCCTATTTTAAAACCAGTTATAAAACAAGCATGTTATTTTTTTA
TCAATCTTGGGAATTCTATT

Downstream 100 bases:

>100_bases
ATTTTAGCGGGCATCAATAAGCATTTTTAATCCGAGAAGCATTAACCAGTATTGCCCAAACAACCCTGGATAACTTAAAC
TGTTTTGCTTCAGGTAAACC

Product: fermentative D-lactate dehydrogenase, NAD-dependent

Products: NA

Alternate protein names: D-LDH; Fermentative lactate dehydrogenase [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MKVAMFSTRNYDIKHFDPLLAVQQESIQIKYFDTRLDIHTSQLAKGYDAVCAFVNDDLAGPVLTELASLGIKLIVMRCAG
FNNINLAVSKKLGLTIMRVPAYSPEAVAEHAIALMMTLNRRIHKAYQRTRDANFSLDGLVGFNMFGKTMGVIGTGKIGLA
TMRILKGFGCEILAYDPYPSAQAIELGANYVSLTELFTRSDAITLHCPLTDDNYHLLNRESFAQMKEGVMIINTSRGALI
NAKDSIDALKSKKIGALGLDVYEEEDSLFFDDHSNEIITDDVFRRLSSCHNVIFTGHQAFLTREALTSIAQTTLDNLNCF
ASSKPNGNEVS

Sequences:

>Translated_331_residues
MKVAMFSTRNYDIKHFDPLLAVQQESIQIKYFDTRLDIHTSQLAKGYDAVCAFVNDDLAGPVLTELASLGIKLIVMRCAG
FNNINLAVSKKLGLTIMRVPAYSPEAVAEHAIALMMTLNRRIHKAYQRTRDANFSLDGLVGFNMFGKTMGVIGTGKIGLA
TMRILKGFGCEILAYDPYPSAQAIELGANYVSLTELFTRSDAITLHCPLTDDNYHLLNRESFAQMKEGVMIINTSRGALI
NAKDSIDALKSKKIGALGLDVYEEEDSLFFDDHSNEIITDDVFRRLSSCHNVIFTGHQAFLTREALTSIAQTTLDNLNCF
ASSKPNGNEVS
>Mature_331_residues
MKVAMFSTRNYDIKHFDPLLAVQQESIQIKYFDTRLDIHTSQLAKGYDAVCAFVNDDLAGPVLTELASLGIKLIVMRCAG
FNNINLAVSKKLGLTIMRVPAYSPEAVAEHAIALMMTLNRRIHKAYQRTRDANFSLDGLVGFNMFGKTMGVIGTGKIGLA
TMRILKGFGCEILAYDPYPSAQAIELGANYVSLTELFTRSDAITLHCPLTDDNYHLLNRESFAQMKEGVMIINTSRGALI
NAKDSIDALKSKKIGALGLDVYEEEDSLFFDDHSNEIITDDVFRRLSSCHNVIFTGHQAFLTREALTSIAQTTLDNLNCF
ASSKPNGNEVS

Specific function: Fermentative lactate dehydrogenase [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI61743967, Length=245, Percent_Identity=33.0612244897959, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI4557497, Length=245, Percent_Identity=33.0612244897959, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI23308577, Length=201, Percent_Identity=30.8457711442786, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI6912396, Length=247, Percent_Identity=31.5789473684211, Blast_Score=114, Evalue=9e-26,
Organism=Homo sapiens, GI145580578, Length=245, Percent_Identity=30.2040816326531, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI4557499, Length=245, Percent_Identity=30.2040816326531, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI145580575, Length=245, Percent_Identity=30.2040816326531, Blast_Score=113, Evalue=3e-25,
Organism=Escherichia coli, GI1787645, Length=330, Percent_Identity=62.4242424242424, Blast_Score=437, Evalue=1e-124,
Organism=Escherichia coli, GI87082289, Length=245, Percent_Identity=30.6122448979592, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1789279, Length=238, Percent_Identity=25.6302521008403, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788660, Length=207, Percent_Identity=27.536231884058, Blast_Score=61, Evalue=1e-10,
Organism=Caenorhabditis elegans, GI25147481, Length=248, Percent_Identity=32.258064516129, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17532191, Length=253, Percent_Identity=27.2727272727273, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6324055, Length=198, Percent_Identity=34.3434343434343, Blast_Score=116, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6320925, Length=237, Percent_Identity=30.379746835443, Blast_Score=109, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6322116, Length=237, Percent_Identity=29.957805907173, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324964, Length=274, Percent_Identity=24.8175182481752, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6324980, Length=201, Percent_Identity=25.8706467661692, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24646446, Length=250, Percent_Identity=31.2, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24646448, Length=250, Percent_Identity=31.2, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24646452, Length=250, Percent_Identity=31.2, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24646450, Length=250, Percent_Identity=31.2, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI62472511, Length=250, Percent_Identity=31.2, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI19921140, Length=309, Percent_Identity=25.5663430420712, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI28574286, Length=253, Percent_Identity=28.8537549407115, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI45552429, Length=215, Percent_Identity=28.3720930232558, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24585514, Length=215, Percent_Identity=28.3720930232558, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI28574282, Length=215, Percent_Identity=28.3720930232558, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI28574284, Length=215, Percent_Identity=28.3720930232558, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI45551003, Length=215, Percent_Identity=28.3720930232558, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI28571528, Length=268, Percent_Identity=27.2388059701493, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24585516, Length=228, Percent_Identity=25, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.28 [H]

Molecular weight: Translated: 36565; Mature: 36565

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVAMFSTRNYDIKHFDPLLAVQQESIQIKYFDTRLDIHTSQLAKGYDAVCAFVNDDLAG
CEEEEEECCCCCCHHCCHHHHHHCCCEEEEEEEEEEEEEHHHHHCCHHHEEEECCCCCCC
PVLTELASLGIKLIVMRCAGFNNINLAVSKKLGLTIMRVPAYSPEAVAEHAIALMMTLNR
HHHHHHHHHHHHEEEEHHCCCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHH
RIHKAYQRTRDANFSLDGLVGFNMFGKTMGVIGTGKIGLATMRILKGFGCEILAYDPYPS
HHHHHHHHHCCCCCCCCCEEEHHHHHHHHCEEECCCHHHHHHHHHHCCCCEEEEECCCCC
AQAIELGANYVSLTELFTRSDAITLHCPLTDDNYHLLNRESFAQMKEGVMIINTSRGALI
CCEEECCCCHHHHHHHHHCCCEEEEEEECCCCCCEEECHHHHHHHHCCEEEEECCCCCEE
NAKDSIDALKSKKIGALGLDVYEEEDSLFFDDHSNEIITDDVFRRLSSCHNVIFTGHQAF
ECHHHHHHHHHCCCCEEECEEEECCCCEEEECCCCCEEHHHHHHHHHHHCCEEEECHHHH
LTREALTSIAQTTLDNLNCFASSKPNGNEVS
HHHHHHHHHHHHHHHCCEEEECCCCCCCCCC
>Mature Secondary Structure
MKVAMFSTRNYDIKHFDPLLAVQQESIQIKYFDTRLDIHTSQLAKGYDAVCAFVNDDLAG
CEEEEEECCCCCCHHCCHHHHHHCCCEEEEEEEEEEEEEHHHHHCCHHHEEEECCCCCCC
PVLTELASLGIKLIVMRCAGFNNINLAVSKKLGLTIMRVPAYSPEAVAEHAIALMMTLNR
HHHHHHHHHHHHEEEEHHCCCCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHH
RIHKAYQRTRDANFSLDGLVGFNMFGKTMGVIGTGKIGLATMRILKGFGCEILAYDPYPS
HHHHHHHHHCCCCCCCCCEEEHHHHHHHHCEEECCCHHHHHHHHHHCCCCEEEEECCCCC
AQAIELGANYVSLTELFTRSDAITLHCPLTDDNYHLLNRESFAQMKEGVMIINTSRGALI
CCEEECCCCHHHHHHHHHCCCEEEEEEECCCCCCEEECHHHHHHHHCCEEEEECCCCCEE
NAKDSIDALKSKKIGALGLDVYEEEDSLFFDDHSNEIITDDVFRRLSSCHNVIFTGHQAF
ECHHHHHHHHHCCCCEEECEEEECCCCEEEECCCCCEEHHHHHHHHHHHCCEEEECHHHH
LTREALTSIAQTTLDNLNCFASSKPNGNEVS
HHHHHHHHHHHHHHHCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9025293; 9097039; 9278503; 8349564 [H]