Click here to switch to the map view.

The map label for this gene is rsmB [H]

Identifier: 119943873

GI number: 119943873

Start: 106392

End: 107702

Strand: Direct

Name: rsmB [H]

Synonym: Ping_0080

Alternate gene names: 119943873

Gene position: 106392-107702 (Clockwise)

Preceding gene: 119943872

Following gene: 119943874

Centisome position: 2.33

GC content: 43.1

Gene sequence:

>1311_bases
ATGAATGTAAGAGCATTAGCAGCTAAAATTTTAAATCAAGTGGTAGAGCAGGGGCAATCATTATCCAATGTCTTACCCCC
TGTCCAACAGGATCTTTCTCCTAAAGATAAAGCATTATTACAGGTACTTTGTTACGGCGTTTTACGTACTTTGCCGCGCT
TAGATTTTTTCTGCCGTTCTCTGATGGACAAAGCATTAAAGGGCAAGCAGCGCGACCTGCACTTCCTTATTTTAGTCGGT
TTTTACCAGTTACTGTATACCCGAATTCCCAGCCATGCCGCGGTTGGAGAAACAGTTAATGGGGCGAAAGAACTTAAAAA
ACAGGCATTAAAAGGCATGGTTAACGGTGTGTTACGGAATTTTTTACGTGAGCAGGAGAGCTTGGTAGAAAAAGCGAATA
AACAACCGGCACTGGTTTATTGCCATCCAAGTTGGCTCGTAAAACGTTTACAAGCAGCCTATGGTGATGAGCTGGCCGCT
CAGATTATGCACAATAATAATCAGCAGGCACCAATGTGGCTACGGGTAAATGCGCTTCATCATACTCGCGATCAATACCA
GGTTCTGTTAAATGATGCCGGACTGCTCAGTTTTAGCTCGGAGTTTAATGATAATGCACTGCGTTTAGAAAAACCGACCG
ATGTTTACAAATTGCCTGGTTTTACCGAGGGCTGGGTTTCAGTGCAGGATGGCGCAGCACAGCTGGCGGCCCATTATTTA
GATGCACAAGCCGGCGATCTGGTTTTAGATGCTTGTTCAGCACCTGGTGGTAAAACAGTGCATGTTCTGGAGCTGCAGAA
AAATATAAAACAGATGGTGGCTGTTGATGCAGATGCTAAACGGTTACTGCGTGTTCAAGAGAATTTAGATCGTCTTAAAC
TTAAGGCAAGCGTTATTCACGGTGATGCATCACAACCGGGCAGTTGGTGGCATGGTGAGCTGTTTGATCGTATTCTTTGC
GATGCACCCTGCAGTGCTACCGGTGTTATTCGTCGTCATCCGGACATCCGTTGGTTACGCCGAGAGAGTGATATTGCAGG
TTTAGTGAGCTTACAAAAAAATATTTTGCATGCGTTATGGGATAAACTAAAACCCGGAGGTATTCTACTCTATGCAACTT
GCTCTATTTTGCCCGATGAAAATGATTTACAAATTCAAGACTTTTTAGAAAGTAGGCAGGATGCCTCGTTAATACCCCTT
ATTGTGCAGGATAATCTCCAAAACAATATCCAAAATAAAGGACGGCAGATATTGCCTAATGAAGATGCGATGGATGGTTT
TTATTATGCAAAATTGCAAAAGTCATTATAA

Upstream 100 bases:

>100_bases
CTTCAGTTGGCGGGAAAAAAAGCGCTGCCGGTACAAGATATTTTAAATTCTCGTCGTGAATTGTTTGCGCAGGGTACTTT
ACTACAGCAAGGTTAATTAC

Downstream 100 bases:

>100_bases
AAGATGCTGCTAATGAGCAATTATGCAAGTCTGTTCATTTACTACTAAACTAAATGACATTTCTTAGCTTGTGGTTATTT
GTGCTATCCATCGATCACTT

Product: sun protein

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MNVRALAAKILNQVVEQGQSLSNVLPPVQQDLSPKDKALLQVLCYGVLRTLPRLDFFCRSLMDKALKGKQRDLHFLILVG
FYQLLYTRIPSHAAVGETVNGAKELKKQALKGMVNGVLRNFLREQESLVEKANKQPALVYCHPSWLVKRLQAAYGDELAA
QIMHNNNQQAPMWLRVNALHHTRDQYQVLLNDAGLLSFSSEFNDNALRLEKPTDVYKLPGFTEGWVSVQDGAAQLAAHYL
DAQAGDLVLDACSAPGGKTVHVLELQKNIKQMVAVDADAKRLLRVQENLDRLKLKASVIHGDASQPGSWWHGELFDRILC
DAPCSATGVIRRHPDIRWLRRESDIAGLVSLQKNILHALWDKLKPGGILLYATCSILPDENDLQIQDFLESRQDASLIPL
IVQDNLQNNIQNKGRQILPNEDAMDGFYYAKLQKSL

Sequences:

>Translated_436_residues
MNVRALAAKILNQVVEQGQSLSNVLPPVQQDLSPKDKALLQVLCYGVLRTLPRLDFFCRSLMDKALKGKQRDLHFLILVG
FYQLLYTRIPSHAAVGETVNGAKELKKQALKGMVNGVLRNFLREQESLVEKANKQPALVYCHPSWLVKRLQAAYGDELAA
QIMHNNNQQAPMWLRVNALHHTRDQYQVLLNDAGLLSFSSEFNDNALRLEKPTDVYKLPGFTEGWVSVQDGAAQLAAHYL
DAQAGDLVLDACSAPGGKTVHVLELQKNIKQMVAVDADAKRLLRVQENLDRLKLKASVIHGDASQPGSWWHGELFDRILC
DAPCSATGVIRRHPDIRWLRRESDIAGLVSLQKNILHALWDKLKPGGILLYATCSILPDENDLQIQDFLESRQDASLIPL
IVQDNLQNNIQNKGRQILPNEDAMDGFYYAKLQKSL
>Mature_436_residues
MNVRALAAKILNQVVEQGQSLSNVLPPVQQDLSPKDKALLQVLCYGVLRTLPRLDFFCRSLMDKALKGKQRDLHFLILVG
FYQLLYTRIPSHAAVGETVNGAKELKKQALKGMVNGVLRNFLREQESLVEKANKQPALVYCHPSWLVKRLQAAYGDELAA
QIMHNNNQQAPMWLRVNALHHTRDQYQVLLNDAGLLSFSSEFNDNALRLEKPTDVYKLPGFTEGWVSVQDGAAQLAAHYL
DAQAGDLVLDACSAPGGKTVHVLELQKNIKQMVAVDADAKRLLRVQENLDRLKLKASVIHGDASQPGSWWHGELFDRILC
DAPCSATGVIRRHPDIRWLRRESDIAGLVSLQKNILHALWDKLKPGGILLYATCSILPDENDLQIQDFLESRQDASLIPL
IVQDNLQNNIQNKGRQILPNEDAMDGFYYAKLQKSL

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=342, Percent_Identity=30.1169590643275, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI76150623, Length=342, Percent_Identity=30.1169590643275, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI32698918, Length=208, Percent_Identity=29.3269230769231, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI270288818, Length=192, Percent_Identity=32.2916666666667, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI23199998, Length=192, Percent_Identity=32.2916666666667, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI270288816, Length=187, Percent_Identity=32.620320855615, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI8922322, Length=192, Percent_Identity=32.2916666666667, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI40316918, Length=205, Percent_Identity=29.2682926829268, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI39995082, Length=189, Percent_Identity=30.6878306878307, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI301336155, Length=189, Percent_Identity=30.6878306878307, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI11545785, Length=235, Percent_Identity=28.0851063829787, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI2367212, Length=433, Percent_Identity=54.2725173210162, Blast_Score=467, Evalue=1e-133,
Organism=Escherichia coli, GI87081985, Length=219, Percent_Identity=28.310502283105, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17536757, Length=334, Percent_Identity=28.4431137724551, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI25143471, Length=177, Percent_Identity=34.4632768361582, Blast_Score=75, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI71998419, Length=414, Percent_Identity=25.8454106280193, Blast_Score=72, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI115534021, Length=184, Percent_Identity=30.4347826086957, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324268, Length=341, Percent_Identity=27.2727272727273, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6319447, Length=234, Percent_Identity=32.0512820512821, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI22024126, Length=293, Percent_Identity=28.3276450511945, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24668781, Length=266, Percent_Identity=29.3233082706767, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21356513, Length=260, Percent_Identity=29.6153846153846, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21355201, Length=218, Percent_Identity=29.3577981651376, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19920494, Length=166, Percent_Identity=31.3253012048193, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 48898; Mature: 48898

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVRALAAKILNQVVEQGQSLSNVLPPVQQDLSPKDKALLQVLCYGVLRTLPRLDFFCRS
CCHHHHHHHHHHHHHHCCCHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LMDKALKGKQRDLHFLILVGFYQLLYTRIPSHAAVGETVNGAKELKKQALKGMVNGVLRN
HHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
FLREQESLVEKANKQPALVYCHPSWLVKRLQAAYGDELAAQIMHNNNQQAPMWLRVNALH
HHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHH
HTRDQYQVLLNDAGLLSFSSEFNDNALRLEKPTDVYKLPGFTEGWVSVQDGAAQLAAHYL
HCHHHHHHHHCCCCCEEECCCCCCCCEEECCCCCCEECCCCCCCCEEECHHHHHHHHHHH
DAQAGDLVLDACSAPGGKTVHVLELQKNIKQMVAVDADAKRLLRVQENLDRLKLKASVIH
CCCCCCCEEECCCCCCCCEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEE
GDASQPGSWWHGELFDRILCDAPCSATGVIRRHPDIRWLRRESDIAGLVSLQKNILHALW
CCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DKLKPGGILLYATCSILPDENDLQIQDFLESRQDASLIPLIVQDNLQNNIQNKGRQILPN
HHCCCCCEEEEEEEEECCCCCCCHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCCCCCCCC
EDAMDGFYYAKLQKSL
CCCCCHHHHHHHHHCC
>Mature Secondary Structure
MNVRALAAKILNQVVEQGQSLSNVLPPVQQDLSPKDKALLQVLCYGVLRTLPRLDFFCRS
CCHHHHHHHHHHHHHHCCCHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LMDKALKGKQRDLHFLILVGFYQLLYTRIPSHAAVGETVNGAKELKKQALKGMVNGVLRN
HHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
FLREQESLVEKANKQPALVYCHPSWLVKRLQAAYGDELAAQIMHNNNQQAPMWLRVNALH
HHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHH
HTRDQYQVLLNDAGLLSFSSEFNDNALRLEKPTDVYKLPGFTEGWVSVQDGAAQLAAHYL
HCHHHHHHHHCCCCCEEECCCCCCCCEEECCCCCCEECCCCCCCCEEECHHHHHHHHHHH
DAQAGDLVLDACSAPGGKTVHVLELQKNIKQMVAVDADAKRLLRVQENLDRLKLKASVIH
CCCCCCCEEECCCCCCCCEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEE
GDASQPGSWWHGELFDRILCDAPCSATGVIRRHPDIRWLRRESDIAGLVSLQKNILHALW
CCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
DKLKPGGILLYATCSILPDENDLQIQDFLESRQDASLIPLIVQDNLQNNIQNKGRQILPN
HHCCCCCEEEEEEEEECCCCCCCHHHHHHHCCCCCCEEEEEEHHHHHHHHHHCCCCCCCC
EDAMDGFYYAKLQKSL
CCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]