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The map label for this gene is def2 [H]

Identifier: 119943871

GI number: 119943871

Start: 104839

End: 105342

Strand: Direct

Name: def2 [H]

Synonym: Ping_0078

Alternate gene names: 119943871

Gene position: 104839-105342 (Clockwise)

Preceding gene: 119943862

Following gene: 119943872

Centisome position: 2.3

GC content: 40.28

Gene sequence:

>504_bases
ATGGCTTTACTAGAAGTATTACGCTTTCCAGATAAACGTCTAAGAACGATTGCGAAACCTGTAGAACAGATCACGGTCGA
ACTTAAAAAAACAGCTGAAAACATGATTGAAACCATGTATGAGGAAGAGGGGGTTGGTCTTGCTGCCACGCAAGTAAATT
TCCACCGACGTCTTGTCGTGATTGATGTTTCTGAGCAGCGCAATCAAGCAATGGTGATCATTAACCCCGTTATTGTCGAA
CATTCAGGAGAAGAGATATCGGAAGAGGGTTGCCTTTCCGTTCCTGAAACTAATGCAGAGGTTAAACGTGCCGAGTTTGT
TACCTTAGAATACTTAGATATTGAAGGGAAGCAACAAGTATTAAAGGCTGATGGTTTGCTGGCCGTTTGTCTTCAGCATG
AAATCGATCACCTGAATGGAAAATTATTCATTGACTATCTGTCACCCTTAAAACAAAAACGGATTAAAACAAAACTGGAA
AAATTACAACGACATAACGTATAG

Upstream 100 bases:

>100_bases
ATAACAATCCTTGTCTGATGACTAATAAGACTTTTTTTTATATTCGGAAATGACTAGAATTAGAACTCTTTAATACTAAC
CAATAGGATTAGATAATATC

Downstream 100 bases:

>100_bases
GGTTGGCGATACTTTTTTCGGCTGATAATCAGAGTCGAATAGAGTGCAATATAACAACCAAAAAATATTTATCATTTTTA
CTTTAAGGTTTCTCTTGGAA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MALLEVLRFPDKRLRTIAKPVEQITVELKKTAENMIETMYEEEGVGLAATQVNFHRRLVVIDVSEQRNQAMVIINPVIVE
HSGEEISEEGCLSVPETNAEVKRAEFVTLEYLDIEGKQQVLKADGLLAVCLQHEIDHLNGKLFIDYLSPLKQKRIKTKLE
KLQRHNV

Sequences:

>Translated_167_residues
MALLEVLRFPDKRLRTIAKPVEQITVELKKTAENMIETMYEEEGVGLAATQVNFHRRLVVIDVSEQRNQAMVIINPVIVE
HSGEEISEEGCLSVPETNAEVKRAEFVTLEYLDIEGKQQVLKADGLLAVCLQHEIDHLNGKLFIDYLSPLKQKRIKTKLE
KLQRHNV
>Mature_166_residues
ALLEVLRFPDKRLRTIAKPVEQITVELKKTAENMIETMYEEEGVGLAATQVNFHRRLVVIDVSEQRNQAMVIINPVIVEH
SGEEISEEGCLSVPETNAEVKRAEFVTLEYLDIEGKQQVLKADGLLAVCLQHEIDHLNGKLFIDYLSPLKQKRIKTKLEK
LQRHNV

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=155, Percent_Identity=33.5483870967742, Blast_Score=74, Evalue=5e-14,
Organism=Escherichia coli, GI1789682, Length=164, Percent_Identity=59.7560975609756, Blast_Score=208, Evalue=1e-55,
Organism=Drosophila melanogaster, GI24645726, Length=140, Percent_Identity=32.8571428571429, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19085; Mature: 18954

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLEVLRFPDKRLRTIAKPVEQITVELKKTAENMIETMYEEEGVGLAATQVNFHRRLVV
CHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEE
IDVSEQRNQAMVIINPVIVEHSGEEISEEGCLSVPETNAEVKRAEFVTLEYLDIEGKQQV
EEECCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHEEEEEEEECCCHHHH
LKADGLLAVCLQHEIDHLNGKLFIDYLSPLKQKRIKTKLEKLQRHNV
HHHCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ALLEVLRFPDKRLRTIAKPVEQITVELKKTAENMIETMYEEEGVGLAATQVNFHRRLVV
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEE
IDVSEQRNQAMVIINPVIVEHSGEEISEEGCLSVPETNAEVKRAEFVTLEYLDIEGKQQV
EEECCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHEEEEEEEECCCHHHH
LKADGLLAVCLQHEIDHLNGKLFIDYLSPLKQKRIKTKLEKLQRHNV
HHHCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA