Click here to switch to the map view.

The map label for this gene is rpoE [H]

Identifier: 119943858

GI number: 119943858

Start: 88101

End: 88682

Strand: Direct

Name: rpoE [H]

Synonym: Ping_0065

Alternate gene names: 119943858

Gene position: 88101-88682 (Clockwise)

Preceding gene: 119943856

Following gene: 119943859

Centisome position: 1.93

GC content: 39.0

Gene sequence:

>582_bases
ATGAGTGAGCGAGATTTAGATTTACAACTGGTTCGGCGTATTCAGAACGGGGATCAAGTTGCTTTTACATTGTTAGTTAG
AAAATACCAAACTCGAGTTGCAAATATTTTAACGCGTTATGTGCGCAATACTGGCGATATTGCAGACGTAGCACAAGAGG
TATTTATCAAAGTGCACAACTCCCTCCCCTCTTTTCGTGGAGAGAGTGCCTTTTATACCTGGCTATATAGAATTACCGTT
AATACAGCCAAAAATTATTTAACAAGTCAAAGCCGTCGACCGCCAGCATCTGATATCGATGCAATGGAGGCTGATAGTTT
TGATGGTAGTGATGCGTTAAGGGAAGTTGACAGTCCTGAGGGAATATTAAGATCTCAGGAAGTAAAGAAAGTAATCTTAA
CAACTATTAATGAATTACCCTCAGAACTAAAAGCTGCGATTACGTTTAGGGAAATTGAGGGTATGAGTTATGAGCAAATA
GCAATTATCGAAGATTGTCCTATCGGCACAGTTCGATCTCGTATTTTTAGAGCTCGTGAAATGATAGATAAGCATCTGAA
ACCTCTATTAGCAGAAGATTAA

Upstream 100 bases:

>100_bases
TAATTTTTTTCATTTTTTCGGAACTTTATTTTTTATTCAAAGTCATAAGTGTGGGTGCTAATAGGGCTCACCAACATTAT
TAATAGGTAGAGAAGCCCTA

Downstream 100 bases:

>100_bases
TTGGGAAGCAAAATGAAAGATGTGAATGAACAACTGTCAGCGATTGTTGATAATGAATTTATTGATGAGAAGTTAATTGA
TGAACTACTGATTAATAAAG

Product: RNA polymerase sigma factor RpoE

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 193; Mature: 192

Protein sequence:

>193_residues
MSERDLDLQLVRRIQNGDQVAFTLLVRKYQTRVANILTRYVRNTGDIADVAQEVFIKVHNSLPSFRGESAFYTWLYRITV
NTAKNYLTSQSRRPPASDIDAMEADSFDGSDALREVDSPEGILRSQEVKKVILTTINELPSELKAAITFREIEGMSYEQI
AIIEDCPIGTVRSRIFRAREMIDKHLKPLLAED

Sequences:

>Translated_193_residues
MSERDLDLQLVRRIQNGDQVAFTLLVRKYQTRVANILTRYVRNTGDIADVAQEVFIKVHNSLPSFRGESAFYTWLYRITV
NTAKNYLTSQSRRPPASDIDAMEADSFDGSDALREVDSPEGILRSQEVKKVILTTINELPSELKAAITFREIEGMSYEQI
AIIEDCPIGTVRSRIFRAREMIDKHLKPLLAED
>Mature_192_residues
SERDLDLQLVRRIQNGDQVAFTLLVRKYQTRVANILTRYVRNTGDIADVAQEVFIKVHNSLPSFRGESAFYTWLYRITVN
TAKNYLTSQSRRPPASDIDAMEADSFDGSDALREVDSPEGILRSQEVKKVILTTINELPSELKAAITFREIEGMSYEQIA
IIEDCPIGTVRSRIFRAREMIDKHLKPLLAED

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=190, Percent_Identity=57.8947368421053, Blast_Score=241, Evalue=2e-65,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 22038; Mature: 21907

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERDLDLQLVRRIQNGDQVAFTLLVRKYQTRVANILTRYVRNTGDIADVAQEVFIKVHN
CCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
SLPSFRGESAFYTWLYRITVNTAKNYLTSQSRRPPASDIDAMEADSFDGSDALREVDSPE
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCH
GILRSQEVKKVILTTINELPSELKAAITFREIEGMSYEQIAIIEDCPIGTVRSRIFRARE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEECCCCCHHHHHHHHHHHH
MIDKHLKPLLAED
HHHHHHHHHHCCC
>Mature Secondary Structure 
SERDLDLQLVRRIQNGDQVAFTLLVRKYQTRVANILTRYVRNTGDIADVAQEVFIKVHN
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
SLPSFRGESAFYTWLYRITVNTAKNYLTSQSRRPPASDIDAMEADSFDGSDALREVDSPE
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCH
GILRSQEVKKVILTTINELPSELKAAITFREIEGMSYEQIAIIEDCPIGTVRSRIFRARE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEECCCCCHHHHHHHHHHHH
MIDKHLKPLLAED
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]