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The map label for this gene is dapF

Identifier: 119943833

GI number: 119943833

Start: 58562

End: 59389

Strand: Reverse

Name: dapF

Synonym: Ping_0040

Alternate gene names: 119943833

Gene position: 59389-58562 (Counterclockwise)

Preceding gene: 119943834

Following gene: 119943832

Centisome position: 1.3

GC content: 41.43

Gene sequence:

>828_bases
ATGAATATTCAATTTTCAAAAATGCACGGCTTGGGTAATGACTTTATGGTCATTGATAACGTCACGCAAAATGTATTCCT
TTCTAAAGATACGATCAGCCGTTTGGCGGATCGTAATTTTGGTATTGGTTTTGACCAATTATTAATGATTGAGCCCCCCT
ATGATCCTGAGCTTGATTTTCATTATCGTATTTTTAACGCAGATGGCAGTGAAGTAGAGCAGTGCGGTAATGGTGCGCGT
TGTTTTGCCCGTTTTGTTAAAATGAAAGGTTTGACACAAAAGCGGATTATTAACGTCAGTACCGCCAAGGGCAAAATTCA
GCTCAAAATTGAGCATGACGGTAATATTACTGTGGATATGGGTAAACCTATTCTAGAGCCTGCAAAAGTACCTTTCGAAG
CTCAGAAAGAGGAAAAAACTTATATTCTCCGTGTAGAAGACCATACTGTTCTATGCGGAGTAGTATCAATAGGTAATCCA
CATTGTGTGGTGTTGGTTGACGATATTGATAACGCTCCGGTTGAAACCTTAGGTCCGTTATTAGAAAATCATGAACGTTT
TCCCAACAAGGTCAATGTGGGTTTCTTAGAAATAAAAAGTCGCGATCATGCCCGCTTAAGAGTTTGGGAGCGAGGGGTAG
GTGAAACATTAGCCTGTGGCACTGGAGCTTGTGCGGCGGCCGTCGTGGGTCAATTACAGGATAAATTAAATCCCCACTGT
ACTATTGAATTACCTGGCGGTGAACTGCGCTTATTTTGGCAACCCGGACAAAGTGTAAAAATGACCGGCACAGCTGATTT
TGTCTTTGATGGACAGATTCAAATTTAG

Upstream 100 bases:

>100_bases
GAAGAGCTTAAAGATTTATGGCGCGGAGAGCACATTTTACCAATATAATAGATTTAACAAAGTGAAACCTGCTTTGGCTT
AATTTCAACAACGAGAAATA

Downstream 100 bases:

>100_bases
TCGCCACTCGATATGGCAAAGCATTTCCAGATCTAGAAAGCACATATGGAGATGCCTTGGTTATTCTTAAAAGTGTGATT
TTTCCCCTGCTAGTGATGAA

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MNIQFSKMHGLGNDFMVIDNVTQNVFLSKDTISRLADRNFGIGFDQLLMIEPPYDPELDFHYRIFNADGSEVEQCGNGAR
CFARFVKMKGLTQKRIINVSTAKGKIQLKIEHDGNITVDMGKPILEPAKVPFEAQKEEKTYILRVEDHTVLCGVVSIGNP
HCVVLVDDIDNAPVETLGPLLENHERFPNKVNVGFLEIKSRDHARLRVWERGVGETLACGTGACAAAVVGQLQDKLNPHC
TIELPGGELRLFWQPGQSVKMTGTADFVFDGQIQI

Sequences:

>Translated_275_residues
MNIQFSKMHGLGNDFMVIDNVTQNVFLSKDTISRLADRNFGIGFDQLLMIEPPYDPELDFHYRIFNADGSEVEQCGNGAR
CFARFVKMKGLTQKRIINVSTAKGKIQLKIEHDGNITVDMGKPILEPAKVPFEAQKEEKTYILRVEDHTVLCGVVSIGNP
HCVVLVDDIDNAPVETLGPLLENHERFPNKVNVGFLEIKSRDHARLRVWERGVGETLACGTGACAAAVVGQLQDKLNPHC
TIELPGGELRLFWQPGQSVKMTGTADFVFDGQIQI
>Mature_275_residues
MNIQFSKMHGLGNDFMVIDNVTQNVFLSKDTISRLADRNFGIGFDQLLMIEPPYDPELDFHYRIFNADGSEVEQCGNGAR
CFARFVKMKGLTQKRIINVSTAKGKIQLKIEHDGNITVDMGKPILEPAKVPFEAQKEEKTYILRVEDHTVLCGVVSIGNP
HCVVLVDDIDNAPVETLGPLLENHERFPNKVNVGFLEIKSRDHARLRVWERGVGETLACGTGACAAAVVGQLQDKLNPHC
TIELPGGELRLFWQPGQSVKMTGTADFVFDGQIQI

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=274, Percent_Identity=64.2335766423358, Blast_Score=379, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_PSYIN (A1SQZ9)

Other databases:

- EMBL:   CP000510
- RefSeq:   YP_941513.1
- ProteinModelPortal:   A1SQZ9
- SMR:   A1SQZ9
- STRING:   A1SQZ9
- GeneID:   4623532
- GenomeReviews:   CP000510_GR
- KEGG:   pin:Ping_0040
- eggNOG:   COG0253
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- PhylomeDB:   A1SQZ9
- BioCyc:   PING357804:PING_0040-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 30578; Mature: 30578

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 75-75 ACT_SITE 219-219

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIQFSKMHGLGNDFMVIDNVTQNVFLSKDTISRLADRNFGIGFDQLLMIEPPYDPELDF
CCCEEHHHCCCCCCEEEEECCCCCEEECHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCE
HYRIFNADGSEVEQCGNGARCFARFVKMKGLTQKRIINVSTAKGKIQLKIEHDGNITVDM
EEEEECCCCHHHHHHCCHHHHHHHHHHHCCCCHHHEEEEEECCCEEEEEEECCCCEEEEC
GKPILEPAKVPFEAQKEEKTYILRVEDHTVLCGVVSIGNPHCVVLVDDIDNAPVETLGPL
CCCCCCCCCCCCCCCCCCCEEEEEEECCEEEEEEEECCCCEEEEEEECCCCCCHHHHHHH
LENHERFPNKVNVGFLEIKSRDHARLRVWERGVGETLACGTGACAAAVVGQLQDKLNPHC
HHHHHCCCCEECEEEEEEECCCCCEEEEECCCCCCEEECCCCHHHHHHHHHHHHHCCCCE
TIELPGGELRLFWQPGQSVKMTGTADFVFDGQIQI
EEEECCCCEEEEECCCCEEEEEECEEEEECCEEEC
>Mature Secondary Structure
MNIQFSKMHGLGNDFMVIDNVTQNVFLSKDTISRLADRNFGIGFDQLLMIEPPYDPELDF
CCCEEHHHCCCCCCEEEEECCCCCEEECHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCE
HYRIFNADGSEVEQCGNGARCFARFVKMKGLTQKRIINVSTAKGKIQLKIEHDGNITVDM
EEEEECCCCHHHHHHCCHHHHHHHHHHHCCCCHHHEEEEEECCCEEEEEEECCCCEEEEC
GKPILEPAKVPFEAQKEEKTYILRVEDHTVLCGVVSIGNPHCVVLVDDIDNAPVETLGPL
CCCCCCCCCCCCCCCCCCCEEEEEEECCEEEEEEEECCCCEEEEEEECCCCCCHHHHHHH
LENHERFPNKVNVGFLEIKSRDHARLRVWERGVGETLACGTGACAAAVVGQLQDKLNPHC
HHHHHCCCCEECEEEEEEECCCCCEEEEECCCCCCEEECCCCHHHHHHHHHHHHHCCCCE
TIELPGGELRLFWQPGQSVKMTGTADFVFDGQIQI
EEEECCCCEEEEECCCCEEEEEECEEEEECCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA