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The map label for this gene is gyrA [H]

Identifier: 119871456

GI number: 119871456

Start: 5665612

End: 5667747

Strand: Direct

Name: gyrA [H]

Synonym: Mkms_5432

Alternate gene names: 119871456

Gene position: 5665612-5667747 (Clockwise)

Preceding gene: 119871455

Following gene: 119871457

Centisome position: 98.75

GC content: 69.43

Gene sequence:

>2136_bases
GTGACCGCGATCATCGAGCAGAACCCCGACCTGGTCCTCGACCAGAGCGCCGACGACTACTGGAACCACTACCAGCTGAC
CTTCGCGCTCTACAGCGTCAGCGACCGCGCCATCCCGTCGGCGTTCGACGGGCTCAAGCCGGGTCAGCGCCGTCTGCTCT
ACCAGATGCACGACTCGAAACTGCTGCCCGGCAACAAACCGCAGAAGTCCTCGAAGGTCTGCTCGGCCGTCACCGGCAAC
CTGCACCCGCACGGCGGCGCGTCGATGTACGGCGCGGCGGCGCTGATGGCCGCCGACTTCCAGCGCGTGAAGGTCATCGA
CGGACAGGGCGCCTTCCCGCGCATCCAGGGCGACATCCCGGCCGCCGACCGCTACACCGAGATGCGGCTGTCCGCCCCCG
GCGCCGCGCTCACCGCCGAACTCGACAGCCATGCCGTGCCGATGGTGGCGACCTTCGACGGCGAGTGGACCGAGCCCACG
GTGCTGCCGGCGCAGTGGCCGGTGCTGCTGTGCAACGGTGCGGTGGGGATCGCCGAAGGGTGGGCCACCAAGGTCCCGGC
CCACAATCCGCGCGAGGTCATGGCCGCCTGCCGCGCACTGCTGAAGACCCCGAACATGACCGACGACCGCCTGGTCAAGC
TCATACCCGGCCCGGACTGGGGCTGCGGCGCGACGGTGGTCGGCGAGACCGGCCTGCGTGAGTACATCACCACCGGCCGG
GGTCAGTTCACCGTGCGCGGCACCGTGACCGTCGACGGGAAGAACTGCGTCGTCACCGAACTGCCGCCAGGTGTCGCGAG
CAACACTGTGCAGGAACGGATCCGGGCCCTGGTGGAGTCCGGCGAGATGTCCGGCGTCGCCGACATGTCGGATCTGACCG
ACCGCCGCAACGGGCTGCGGATCGTCGTCACCGCCAAGCGCGGCCACAGCGCCGAGCAGATCCGCGAGCAGCTGCTGGCG
CTGACGCCGCTGGAGTCGACGTTCGCCGCGAGCCTCGTCGCCCTCGACGAGAACCGGGTGCCGCGCTGGTGGTCGGTCCG
TGAGCTGATCGCCGCGTTCCTGTCCCTGCGCGACAAGGTGGTGCTGCACCGCAGCGAGTACCGGTTGGAGAAGGTCACCG
CGCGGCGCCATCTGGTGGCCGGCCTGATGCTGATCCACCTGGACATCGACGCCGCCGTGGCGGTCATCCGTGGCTCCGAC
ACCGTCGACGAGGCCCGCCAGGGCCTGCAGGACCGGTTCGCCATCGACGCCGTCCAGGCCGACTACGTGCTGGCGTTGCA
GCTGCGCCGCCTGACCAAGCTCGACGTCATCGAACTGCAGGCCGAGGCCGAGAGGCTGGACGCCGAATTCGCCGAACTCA
CTGAACTGGTGTCGAATCCGGATGCCCGCCGGGTGGTGATCGACAAGGAGTTGGTCGAGACCGCGAAGCTGTTCAAGGGC
CCCGAGTTCGACCGCCGCACCGTGCTGGACTTCGAGGCCATCCCGATCACCACCGGCGCCGCCGACGACGGACCCCGTGA
ACGTAAGGTCAACGCGTCCTGGCGACTCGACGACCGTGGTGTGTTCTCCGACAGCCACGGCGATTTGCTCACCTCGGGTC
TGGGCTGGGCGGTGTGGACCGACGGACGGGTGAAGTTCACCACCGGAGCCGGCCTGCCCTACAAGATCCGCGACATCCCG
GTGGCCCCGGACATCACCGGGTTGCTGCGCTCGGGCGTGCTGCCCGACGGGGCCCATCTGGCATTGGTGACCCGGCGCGG
CAAGATCCTGCGCATCGACCCGGCTGCGGTGAATCCGCAGGGCGCGGCGGGCAATGGGGTGGCCGGGGTGAAGCTCGCGG
CCGGTGACCCCATGGACACGGTGATCGCCGCGCTCCCACTGACCTGCGGCAACGACGAGGCCATCCTGTCGATATCCGAA
AAGGGTTGGAAGGTCACCGAAGTGCCGGACATCCCGGTCAAGGGCCGCGGCGGCGCCGGGGTCGGGTTCCACCCGTTCGT
CGGCGGGGAGGATGCGCTGCTGTCGGCGTCGGTGTCGCCGACCGGGTTCATGCGCGGCAAGAAGAAGGTGCGGGCCGAGA
ACCGCGCCAAGGCGTCCGTCAAGGGTTCCGGTGACGGGGTGACGCCGGCCGAGTAG

Upstream 100 bases:

>100_bases
ACTCTGTTCGGCGGGCCGCCAGAGGGCAGGCGCACGTGGATGGCCGATGTGGCCTCCCGTGTCGACACCACTGCGCTCGA
CCTGAGCTAGGAGACACACT

Downstream 100 bases:

>100_bases
CCGGCTCGCGGACCGCGGCCGAGACGTCGACGCCCGGCGACGTCGGGCTTCAATGTCGGTTCATGCGGCTGGATTTCAGC
GATACCGCGGACCCTCACGG

Product: DNA gyrase/topoisomerase IV subunit A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 711; Mature: 710

Protein sequence:

>711_residues
MTAIIEQNPDLVLDQSADDYWNHYQLTFALYSVSDRAIPSAFDGLKPGQRRLLYQMHDSKLLPGNKPQKSSKVCSAVTGN
LHPHGGASMYGAAALMAADFQRVKVIDGQGAFPRIQGDIPAADRYTEMRLSAPGAALTAELDSHAVPMVATFDGEWTEPT
VLPAQWPVLLCNGAVGIAEGWATKVPAHNPREVMAACRALLKTPNMTDDRLVKLIPGPDWGCGATVVGETGLREYITTGR
GQFTVRGTVTVDGKNCVVTELPPGVASNTVQERIRALVESGEMSGVADMSDLTDRRNGLRIVVTAKRGHSAEQIREQLLA
LTPLESTFAASLVALDENRVPRWWSVRELIAAFLSLRDKVVLHRSEYRLEKVTARRHLVAGLMLIHLDIDAAVAVIRGSD
TVDEARQGLQDRFAIDAVQADYVLALQLRRLTKLDVIELQAEAERLDAEFAELTELVSNPDARRVVIDKELVETAKLFKG
PEFDRRTVLDFEAIPITTGAADDGPRERKVNASWRLDDRGVFSDSHGDLLTSGLGWAVWTDGRVKFTTGAGLPYKIRDIP
VAPDITGLLRSGVLPDGAHLALVTRRGKILRIDPAAVNPQGAAGNGVAGVKLAAGDPMDTVIAALPLTCGNDEAILSISE
KGWKVTEVPDIPVKGRGGAGVGFHPFVGGEDALLSASVSPTGFMRGKKKVRAENRAKASVKGSGDGVTPAE

Sequences:

>Translated_711_residues
MTAIIEQNPDLVLDQSADDYWNHYQLTFALYSVSDRAIPSAFDGLKPGQRRLLYQMHDSKLLPGNKPQKSSKVCSAVTGN
LHPHGGASMYGAAALMAADFQRVKVIDGQGAFPRIQGDIPAADRYTEMRLSAPGAALTAELDSHAVPMVATFDGEWTEPT
VLPAQWPVLLCNGAVGIAEGWATKVPAHNPREVMAACRALLKTPNMTDDRLVKLIPGPDWGCGATVVGETGLREYITTGR
GQFTVRGTVTVDGKNCVVTELPPGVASNTVQERIRALVESGEMSGVADMSDLTDRRNGLRIVVTAKRGHSAEQIREQLLA
LTPLESTFAASLVALDENRVPRWWSVRELIAAFLSLRDKVVLHRSEYRLEKVTARRHLVAGLMLIHLDIDAAVAVIRGSD
TVDEARQGLQDRFAIDAVQADYVLALQLRRLTKLDVIELQAEAERLDAEFAELTELVSNPDARRVVIDKELVETAKLFKG
PEFDRRTVLDFEAIPITTGAADDGPRERKVNASWRLDDRGVFSDSHGDLLTSGLGWAVWTDGRVKFTTGAGLPYKIRDIP
VAPDITGLLRSGVLPDGAHLALVTRRGKILRIDPAAVNPQGAAGNGVAGVKLAAGDPMDTVIAALPLTCGNDEAILSISE
KGWKVTEVPDIPVKGRGGAGVGFHPFVGGEDALLSASVSPTGFMRGKKKVRAENRAKASVKGSGDGVTPAE
>Mature_710_residues
TAIIEQNPDLVLDQSADDYWNHYQLTFALYSVSDRAIPSAFDGLKPGQRRLLYQMHDSKLLPGNKPQKSSKVCSAVTGNL
HPHGGASMYGAAALMAADFQRVKVIDGQGAFPRIQGDIPAADRYTEMRLSAPGAALTAELDSHAVPMVATFDGEWTEPTV
LPAQWPVLLCNGAVGIAEGWATKVPAHNPREVMAACRALLKTPNMTDDRLVKLIPGPDWGCGATVVGETGLREYITTGRG
QFTVRGTVTVDGKNCVVTELPPGVASNTVQERIRALVESGEMSGVADMSDLTDRRNGLRIVVTAKRGHSAEQIREQLLAL
TPLESTFAASLVALDENRVPRWWSVRELIAAFLSLRDKVVLHRSEYRLEKVTARRHLVAGLMLIHLDIDAAVAVIRGSDT
VDEARQGLQDRFAIDAVQADYVLALQLRRLTKLDVIELQAEAERLDAEFAELTELVSNPDARRVVIDKELVETAKLFKGP
EFDRRTVLDFEAIPITTGAADDGPRERKVNASWRLDDRGVFSDSHGDLLTSGLGWAVWTDGRVKFTTGAGLPYKIRDIPV
APDITGLLRSGVLPDGAHLALVTRRGKILRIDPAAVNPQGAAGNGVAGVKLAAGDPMDTVIAALPLTCGNDEAILSISEK
GWKVTEVPDIPVKGRGGAGVGFHPFVGGEDALLSASVSPTGFMRGKKKVRAENRAKASVKGSGDGVTPAE

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0188

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the topoisomerase gyrA/parC subunit family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=353, Percent_Identity=24.3626062322946, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI19913406, Length=364, Percent_Identity=23.3516483516484, Blast_Score=77, Evalue=5e-14,
Organism=Escherichia coli, GI1789396, Length=477, Percent_Identity=33.7526205450734, Blast_Score=254, Evalue=1e-68,
Organism=Escherichia coli, GI1788562, Length=734, Percent_Identity=28.8828337874659, Blast_Score=239, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI212645845, Length=440, Percent_Identity=22.0454545454545, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17535065, Length=355, Percent_Identity=25.6338028169014, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020899
- InterPro:   IPR005743
- InterPro:   IPR006691
- InterPro:   IPR002205
- InterPro:   IPR013758
- InterPro:   IPR013757
- InterPro:   IPR013760 [H]

Pfam domain/function: PF03989 DNA_gyraseA_C; PF00521 DNA_topoisoIV [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 76479; Mature: 76348

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIIEQNPDLVLDQSADDYWNHYQLTFALYSVSDRAIPSAFDGLKPGQRRLLYQMHDSK
CCEEEECCCCEEEECCCCCCCCEEEEEEEEEECCCCCCCHHHCCCCCHHHHHHHHCCCCC
LLPGNKPQKSSKVCSAVTGNLHPHGGASMYGAAALMAADFQRVKVIDGQGAFPRIQGDIP
CCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCCEECCCCC
AADRYTEMRLSAPGAALTAELDSHAVPMVATFDGEWTEPTVLPAQWPVLLCNGAVGIAEG
CCCCCEEEEECCCCCEEEEECCCCCCCEEEECCCCCCCCEECCCCCCEEEECCCCEECCC
WATKVPAHNPREVMAACRALLKTPNMTDDRLVKLIPGPDWGCGATVVGETGLREYITTGR
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEECCCHHHHHHHCCC
GQFTVRGTVTVDGKNCVVTELPPGVASNTVQERIRALVESGEMSGVADMSDLTDRRNGLR
CEEEEEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCEE
IVVTAKRGHSAEQIREQLLALTPLESTFAASLVALDENRVPRWWSVRELIAAFLSLRDKV
EEEEECCCCCHHHHHHHHHHHCCCHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHE
VLHRSEYRLEKVTARRHLVAGLMLIHLDIDAAVAVIRGSDTVDEARQGLQDRFAIDAVQA
EEEHHHHHHHHHHHHHHHHHEEEEEEEECCCEEEEEECCCCHHHHHHCHHHHHHHHHHCC
DYVLALQLRRLTKLDVIELQAEAERLDAEFAELTELVSNPDARRVVIDKELVETAKLFKG
CHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHCC
PEFDRRTVLDFEAIPITTGAADDGPRERKVNASWRLDDRGVFSDSHGDLLTSGLGWAVWT
CCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHCCCCEEEEE
DGRVKFTTGAGLPYKIRDIPVAPDITGLLRSGVLPDGAHLALVTRRGKILRIDPAAVNPQ
CCEEEEEECCCCCEEEECCCCCCCHHHHHHCCCCCCCCEEEEEECCCCEEEECCCCCCCC
GAAGNGVAGVKLAAGDPMDTVIAALPLTCGNDEAILSISEKGWKVTEVPDIPVKGRGGAG
CCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCEEEEECCCCCEEECCCCCCCCCCCCCC
VGFHPFVGGEDALLSASVSPTGFMRGKKKVRAENRAKASVKGSGDGVTPAE
CCCCCCCCCCCCEEEECCCCCHHHCCHHHHHHCCCCCEEECCCCCCCCCCC
>Mature Secondary Structure 
TAIIEQNPDLVLDQSADDYWNHYQLTFALYSVSDRAIPSAFDGLKPGQRRLLYQMHDSK
CEEEECCCCEEEECCCCCCCCEEEEEEEEEECCCCCCCHHHCCCCCHHHHHHHHCCCCC
LLPGNKPQKSSKVCSAVTGNLHPHGGASMYGAAALMAADFQRVKVIDGQGAFPRIQGDIP
CCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCCCEECCCCC
AADRYTEMRLSAPGAALTAELDSHAVPMVATFDGEWTEPTVLPAQWPVLLCNGAVGIAEG
CCCCCEEEEECCCCCEEEEECCCCCCCEEEECCCCCCCCEECCCCCCEEEECCCCEECCC
WATKVPAHNPREVMAACRALLKTPNMTDDRLVKLIPGPDWGCGATVVGETGLREYITTGR
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCEEECCCHHHHHHHCCC
GQFTVRGTVTVDGKNCVVTELPPGVASNTVQERIRALVESGEMSGVADMSDLTDRRNGLR
CEEEEEEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCEE
IVVTAKRGHSAEQIREQLLALTPLESTFAASLVALDENRVPRWWSVRELIAAFLSLRDKV
EEEEECCCCCHHHHHHHHHHHCCCHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHE
VLHRSEYRLEKVTARRHLVAGLMLIHLDIDAAVAVIRGSDTVDEARQGLQDRFAIDAVQA
EEEHHHHHHHHHHHHHHHHHEEEEEEEECCCEEEEEECCCCHHHHHHCHHHHHHHHHHCC
DYVLALQLRRLTKLDVIELQAEAERLDAEFAELTELVSNPDARRVVIDKELVETAKLFKG
CHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHHHHHHHCC
PEFDRRTVLDFEAIPITTGAADDGPRERKVNASWRLDDRGVFSDSHGDLLTSGLGWAVWT
CCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCCCCCHHHCCCCEEEEE
DGRVKFTTGAGLPYKIRDIPVAPDITGLLRSGVLPDGAHLALVTRRGKILRIDPAAVNPQ
CCEEEEEECCCCCEEEECCCCCCCHHHHHHCCCCCCCCEEEEEECCCCEEEECCCCCCCC
GAAGNGVAGVKLAAGDPMDTVIAALPLTCGNDEAILSISEKGWKVTEVPDIPVKGRGGAG
CCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCEEEEECCCCCEEECCCCCCCCCCCCCC
VGFHPFVGGEDALLSASVSPTGFMRGKKKVRAENRAKASVKGSGDGVTPAE
CCCCCCCCCCCCEEEECCCCCHHHCCHHHHHHCCCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]