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The map label for this gene is glnT [H]

Identifier: 119871457

GI number: 119871457

Start: 5667962

End: 5669317

Strand: Direct

Name: glnT [H]

Synonym: Mkms_5433

Alternate gene names: 119871457

Gene position: 5667962-5669317 (Clockwise)

Preceding gene: 119871456

Following gene: 119871458

Centisome position: 98.79

GC content: 64.75

Gene sequence:

>1356_bases
ATGGCGCGCGATGCAGCTGAGGTCGAGGTCATGGCCAAGCAGCTCAAGGAAGACGGGGTTCGGTTCAGCATCGCTGCCTA
CACCGATCTGCACGGCAACATCAAAGGAAAGATGGTGCCGATCGATCACTTCGTTCAGATGGCCGGAGGCTCGGAACTGT
TCACCGGGGCGGCGTTGGACGGCCTGCCGCAGGACGTCAGCGACGAGGAGCTCTCCGCGCATCCCGACCTGGACCGGGGG
TTCGTCCTGCCCTGGCGTAAGGACATCGCCCTGTTCCTCTCCGATCTCTACGTCGGAGGCGAGCCGTTCGAGGCATCGAC
GCGAAACATCCTGGGCCGGCAGATGAAAGCGGCCGAGGAGTTGGGATTTGTGTTCAACCTGGGCGTCGAGACCGAGTTCT
TCGTCCTCGAGGACGGGCCCGTAGGCCCGAGCGCGCTGAGCGAGCGCGACACGCTGGAGAAGCCCTGCTACGCGGTTCCC
ATGCTGCTGGACAACTTCGGTTGGCTGCAGGAGCTCGTCGACTACATGAACGAACTCGGTTGGGATGTCTACTCATTCGA
TCACGAGGACGCCCAGGGCCAGTTCGAGATCGACTTCGCCTACGCCGACGGACTGTCGATGGCAGACCGCGTCTCGTTCT
TCCGCTTGATGGCCAACGAGGTCGCGCGCAGACACGGGGCGTACGTCTCCTTCATGCCGAAACCGTTCGCCGACCGCACA
GGCTCCGGTGCGCACTTCAACATGTCACTGGCGGGGCTCGACGACGGGCACAACGTCTTCGAGTCCGCCGACGACCCACG
GGCAGCCGGGCTGAGCGACATCGGCTACCACTTCGCGGCCGGCATCGTCCGGCACGCCGCGGCGATCTGTGCGGTCATCG
CGCCGACGGTCAACAGCTACAAACGGCTGGTGCGCCAGGGCAGCATGTCGGGTTCGACATGGGCACCGATCTTCGCCAGC
TGCGGCAACAACAACCGCACGAACATGCTGCGCATCCCGCTCGGCGGCGGCCGCGTGGAGTGTCGCGCCGTCGATTCGGC
GGCCAACCCCCATCTTGCGGCGGCACTGCTGCTGGCCGCCGGCCTGGAAGGGGTGCGCGAGCAGCTCGAACTGGAGCACT
TCCACACCGACAACCTGTACGAGGTCGACGCCGGCGAATACGAGAAGCGTGGGCTCTCGCTGCTGCCACGCACTCTCGAG
GAGGCGGTGGACGCGTTCGAGGCCGACCCGTTCACCAAGCAGGTGTGCGGGGAGAAGTTGTGGCAGTCGTTCATCGACTA
CAAGCGCAGCGAGTGGACGTCCTATGCCACCGCCGTGACCGATTGGGAGCGCGACCGCTACCTCAAGTTCTTCTGA

Upstream 100 bases:

>100_bases
GACGCTCCGAAATCTCGTTTTAACGTACGCGCCACAGATGAGAAATGACCCGTTCCTATGGTTTCGATAACTGATCGAAA
CTAGGAATGAGGGGTCGACG

Downstream 100 bases:

>100_bases
GCTGCGCAACCAGACGGACGCCAGATCGATGACCATGGATGCCGCTCGCGGCGAACGGAAAGAGCTTCGCGTACCGACGT
TTTCGCTGGAGTCGGGTGCA

Product: L-glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase; Glutamine synthetase III; GSIII [H]

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MARDAAEVEVMAKQLKEDGVRFSIAAYTDLHGNIKGKMVPIDHFVQMAGGSELFTGAALDGLPQDVSDEELSAHPDLDRG
FVLPWRKDIALFLSDLYVGGEPFEASTRNILGRQMKAAEELGFVFNLGVETEFFVLEDGPVGPSALSERDTLEKPCYAVP
MLLDNFGWLQELVDYMNELGWDVYSFDHEDAQGQFEIDFAYADGLSMADRVSFFRLMANEVARRHGAYVSFMPKPFADRT
GSGAHFNMSLAGLDDGHNVFESADDPRAAGLSDIGYHFAAGIVRHAAAICAVIAPTVNSYKRLVRQGSMSGSTWAPIFAS
CGNNNRTNMLRIPLGGGRVECRAVDSAANPHLAAALLLAAGLEGVREQLELEHFHTDNLYEVDAGEYEKRGLSLLPRTLE
EAVDAFEADPFTKQVCGEKLWQSFIDYKRSEWTSYATAVTDWERDRYLKFF

Sequences:

>Translated_451_residues
MARDAAEVEVMAKQLKEDGVRFSIAAYTDLHGNIKGKMVPIDHFVQMAGGSELFTGAALDGLPQDVSDEELSAHPDLDRG
FVLPWRKDIALFLSDLYVGGEPFEASTRNILGRQMKAAEELGFVFNLGVETEFFVLEDGPVGPSALSERDTLEKPCYAVP
MLLDNFGWLQELVDYMNELGWDVYSFDHEDAQGQFEIDFAYADGLSMADRVSFFRLMANEVARRHGAYVSFMPKPFADRT
GSGAHFNMSLAGLDDGHNVFESADDPRAAGLSDIGYHFAAGIVRHAAAICAVIAPTVNSYKRLVRQGSMSGSTWAPIFAS
CGNNNRTNMLRIPLGGGRVECRAVDSAANPHLAAALLLAAGLEGVREQLELEHFHTDNLYEVDAGEYEKRGLSLLPRTLE
EAVDAFEADPFTKQVCGEKLWQSFIDYKRSEWTSYATAVTDWERDRYLKFF
>Mature_450_residues
ARDAAEVEVMAKQLKEDGVRFSIAAYTDLHGNIKGKMVPIDHFVQMAGGSELFTGAALDGLPQDVSDEELSAHPDLDRGF
VLPWRKDIALFLSDLYVGGEPFEASTRNILGRQMKAAEELGFVFNLGVETEFFVLEDGPVGPSALSERDTLEKPCYAVPM
LLDNFGWLQELVDYMNELGWDVYSFDHEDAQGQFEIDFAYADGLSMADRVSFFRLMANEVARRHGAYVSFMPKPFADRTG
SGAHFNMSLAGLDDGHNVFESADDPRAAGLSDIGYHFAAGIVRHAAAICAVIAPTVNSYKRLVRQGSMSGSTWAPIFASC
GNNNRTNMLRIPLGGGRVECRAVDSAANPHLAAALLLAAGLEGVREQLELEHFHTDNLYEVDAGEYEKRGLSLLPRTLEE
AVDAFEADPFTKQVCGEKLWQSFIDYKRSEWTSYATAVTDWERDRYLKFF

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family. Type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=475, Percent_Identity=25.6842105263158, Blast_Score=128, Evalue=1e-29,
Organism=Escherichia coli, GI1790301, Length=436, Percent_Identity=27.2935779816514, Blast_Score=131, Evalue=9e-32,
Organism=Escherichia coli, GI87081870, Length=453, Percent_Identity=27.1523178807947, Blast_Score=130, Evalue=2e-31,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR017536 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 49934; Mature: 49803

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARDAAEVEVMAKQLKEDGVRFSIAAYTDLHGNIKGKMVPIDHFVQMAGGSELFTGAALD
CCCCHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEECHHHHHHHCCCCCEECCHHHC
GLPQDVSDEELSAHPDLDRGFVLPWRKDIALFLSDLYVGGEPFEASTRNILGRQMKAAEE
CCCCCCCCCHHCCCCCCCCCEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
LGFVFNLGVETEFFVLEDGPVGPSALSERDTLEKPCYAVPMLLDNFGWLQELVDYMNELG
CCEEEEECCCEEEEEEECCCCCHHHHCCCHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHC
WDVYSFDHEDAQGQFEIDFAYADGLSMADRVSFFRLMANEVARRHGAYVSFMPKPFADRT
CEEEECCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
GSGAHFNMSLAGLDDGHNVFESADDPRAAGLSDIGYHFAAGIVRHAAAICAVIAPTVNSY
CCCCEEEEEEECCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRLVRQGSMSGSTWAPIFASCGNNNRTNMLRIPLGGGRVECRAVDSAANPHLAAALLLAA
HHHHHHCCCCCCCCCHHHHCCCCCCCCCEEEEECCCCEEEEEEECCCCCHHHHHHHHHHH
GLEGVREQLELEHFHTDNLYEVDAGEYEKRGLSLLPRTLEEAVDAFEADPFTKQVCGEKL
HHHHHHHHHHHHHCCCCCEEECCCCCHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
WQSFIDYKRSEWTSYATAVTDWERDRYLKFF
HHHHHHHHHHHHHHHHHHHHCCCCCCCEECC
>Mature Secondary Structure 
ARDAAEVEVMAKQLKEDGVRFSIAAYTDLHGNIKGKMVPIDHFVQMAGGSELFTGAALD
CCCHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCEEECHHHHHHHCCCCCEECCHHHC
GLPQDVSDEELSAHPDLDRGFVLPWRKDIALFLSDLYVGGEPFEASTRNILGRQMKAAEE
CCCCCCCCCHHCCCCCCCCCEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
LGFVFNLGVETEFFVLEDGPVGPSALSERDTLEKPCYAVPMLLDNFGWLQELVDYMNELG
CCEEEEECCCEEEEEEECCCCCHHHHCCCHHHCCCHHHHHHHHHCHHHHHHHHHHHHHHC
WDVYSFDHEDAQGQFEIDFAYADGLSMADRVSFFRLMANEVARRHGAYVSFMPKPFADRT
CEEEECCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCC
GSGAHFNMSLAGLDDGHNVFESADDPRAAGLSDIGYHFAAGIVRHAAAICAVIAPTVNSY
CCCCEEEEEEECCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KRLVRQGSMSGSTWAPIFASCGNNNRTNMLRIPLGGGRVECRAVDSAANPHLAAALLLAA
HHHHHHCCCCCCCCCHHHHCCCCCCCCCEEEEECCCCEEEEEEECCCCCHHHHHHHHHHH
GLEGVREQLELEHFHTDNLYEVDAGEYEKRGLSLLPRTLEEAVDAFEADPFTKQVCGEKL
HHHHHHHHHHHHHCCCCCEEECCCCCHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
WQSFIDYKRSEWTSYATAVTDWERDRYLKFF
HHHHHHHHHHHHHHHHHHHHCCCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1356885 [H]