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The map label for this gene is pcpB [H]

Identifier: 119871410

GI number: 119871410

Start: 5620503

End: 5622050

Strand: Direct

Name: pcpB [H]

Synonym: Mkms_5386

Alternate gene names: 119871410

Gene position: 5620503-5622050 (Clockwise)

Preceding gene: 119871409

Following gene: 119871413

Centisome position: 97.97

GC content: 70.28

Gene sequence:

>1548_bases
ATGTCGCCCGCCTACCGCGTCGACGACCTCCCGGTCACCGACACCGACGTCCTGATCGTCGGTGCCGGACCGACCGGTCT
GATGGCCGCACTGGCGCTCCACCGCCGCGGCGTACCCGCAGTGCTGGTCGATCGCAAGGCCGGTCCGACCCGGGAATCCC
GGGCGCTGGCGGTGCAGGCCCGCACGATGGAGGTCTACGACCAGCTCGGGCTGGCCGACACGGTGCTGGCGAATTCCAAC
TCCGCGTTGCGGATTCAGGTCGGCGGATCGTCCCGCGGGATCGGTCCGAACCTGGCGAAGCTGCAGGACGGTCACACCCG
CTTTCCTGGCGTCCATATCTTCGAGCAGAGCCGCAACGAGGACATGCTCGCCGGCGCGCTCACCGCGGGCGGCGGCGATA
TCCGCTGGCGGCACCGGCTGGTCGATCTGCAGGACAGGACCGCGGCACCCGACGGGCGGGTGGAGGCACTGCTCGAAGGA
CCGGACGGCACCCTCCTGCGGGTGCGGGCCCGGTGGTGCATCGGTGCCGACGGTGCCGCCTCGGCGGTCCGGCGCGCATT
GAACCTGCCCTTCGAGGGCCGCACCGACGAGGCGACGTTCTGGGTCGCCGACGTCCGTGCCGTCCGCGGTGTACCCGACG
ACGCCGTCAACATCCGACCGGGTAAGCGGACGTTCGCGGCGGTGTTCCCGCTGGGGCCCGGTGGCCACGTCAGGCTGCTG
GGTCTGGCCGCCAAGGACACCATCACGCAGGACGAGGCGCTCGCCACCGTGGCTGCCGAGTTCGGGCTGACACACGGTCC
GGTGGAATGGTTTTCGACCTATCGCGTGCATCACCGCGTGTCGGCGAGGTTTCGCGTGGGGTCGATCTTCCTGGCCGGGG
ACGCCGCACATGTGCACTCCCCCGTCGGCGGTCAGGGTATGAACACCGGCCTGCAGGATGCGCACAACCTGGCGATGCTG
CTGGCCGATGTGGCGCAGAACCGCGTGGACGGACGGGCACTCGACCGCTACGAACGGGAACGGCGCCCGGTCGCGCTGAC
CCTGGTCAACGTCACCGATCGTGCCTTCACCGTCATCGGCCGCCGCGGATCGTCGGCCGGGCTGGTCCGGGCGACCATCG
GCGCCTTGGCATTCGGGGTCTTCCCTCTGGTCACCCGGACATCACTGGGCACCCGCCTCGGCGGCTACATCGGTCAATAC
CGCATCCGCTACCGCTACACAGCGAATCAGACGGAGCGGGAACGTGATCCGGCGGTGGGGCTGCGGCTACCTCCGGTCGC
GGACAATCAGCGCGCCCTCGACACGATGACATGGCAGTTGCACGCCTACGGTGCCGGCACGATCGTCCGCCCCGACGTAC
CGGAGTGGATCGACGGCCCGCGGGAGTTCGGTGCCGATCCGCGCGGCCGGTTGCGGTCCGACCGCCTCTACCTCGTCCGG
CCCGATCAGTTCGTGGCCGCGTCGATCCCGCTGCGGGACAACGTCGTCGACGAGGCGCACCTGCGCGCGGCGCTGGCGAC
CCACCAGATTCTGCACGCCGGACCCTAG

Upstream 100 bases:

>100_bases
CGGTGAACTCGGCGGATTCGCCGGCTACGATCCCGCGGCCGCGCTCGCCACGCTCGCCTGATCGGCCCGGGCCACCGCGA
CTTCGGGCTACGGTGACCGT

Downstream 100 bases:

>100_bases
GCGATCTCGTCGTCTATCACGCCGATGCCGGCTCGGAGGATGCCGAGAAACTGGCGCTTCTGGGGTCAGCGACCGGCGCG
GGTCACCACAGCACCGCGAT

Product: FAD-binding monooxygenase

Products: NA

Alternate protein names: Pentachlorophenol hydroxylase [H]

Number of amino acids: Translated: 515; Mature: 514

Protein sequence:

>515_residues
MSPAYRVDDLPVTDTDVLIVGAGPTGLMAALALHRRGVPAVLVDRKAGPTRESRALAVQARTMEVYDQLGLADTVLANSN
SALRIQVGGSSRGIGPNLAKLQDGHTRFPGVHIFEQSRNEDMLAGALTAGGGDIRWRHRLVDLQDRTAAPDGRVEALLEG
PDGTLLRVRARWCIGADGAASAVRRALNLPFEGRTDEATFWVADVRAVRGVPDDAVNIRPGKRTFAAVFPLGPGGHVRLL
GLAAKDTITQDEALATVAAEFGLTHGPVEWFSTYRVHHRVSARFRVGSIFLAGDAAHVHSPVGGQGMNTGLQDAHNLAML
LADVAQNRVDGRALDRYERERRPVALTLVNVTDRAFTVIGRRGSSAGLVRATIGALAFGVFPLVTRTSLGTRLGGYIGQY
RIRYRYTANQTERERDPAVGLRLPPVADNQRALDTMTWQLHAYGAGTIVRPDVPEWIDGPREFGADPRGRLRSDRLYLVR
PDQFVAASIPLRDNVVDEAHLRAALATHQILHAGP

Sequences:

>Translated_515_residues
MSPAYRVDDLPVTDTDVLIVGAGPTGLMAALALHRRGVPAVLVDRKAGPTRESRALAVQARTMEVYDQLGLADTVLANSN
SALRIQVGGSSRGIGPNLAKLQDGHTRFPGVHIFEQSRNEDMLAGALTAGGGDIRWRHRLVDLQDRTAAPDGRVEALLEG
PDGTLLRVRARWCIGADGAASAVRRALNLPFEGRTDEATFWVADVRAVRGVPDDAVNIRPGKRTFAAVFPLGPGGHVRLL
GLAAKDTITQDEALATVAAEFGLTHGPVEWFSTYRVHHRVSARFRVGSIFLAGDAAHVHSPVGGQGMNTGLQDAHNLAML
LADVAQNRVDGRALDRYERERRPVALTLVNVTDRAFTVIGRRGSSAGLVRATIGALAFGVFPLVTRTSLGTRLGGYIGQY
RIRYRYTANQTERERDPAVGLRLPPVADNQRALDTMTWQLHAYGAGTIVRPDVPEWIDGPREFGADPRGRLRSDRLYLVR
PDQFVAASIPLRDNVVDEAHLRAALATHQILHAGP
>Mature_514_residues
SPAYRVDDLPVTDTDVLIVGAGPTGLMAALALHRRGVPAVLVDRKAGPTRESRALAVQARTMEVYDQLGLADTVLANSNS
ALRIQVGGSSRGIGPNLAKLQDGHTRFPGVHIFEQSRNEDMLAGALTAGGGDIRWRHRLVDLQDRTAAPDGRVEALLEGP
DGTLLRVRARWCIGADGAASAVRRALNLPFEGRTDEATFWVADVRAVRGVPDDAVNIRPGKRTFAAVFPLGPGGHVRLLG
LAAKDTITQDEALATVAAEFGLTHGPVEWFSTYRVHHRVSARFRVGSIFLAGDAAHVHSPVGGQGMNTGLQDAHNLAMLL
ADVAQNRVDGRALDRYERERRPVALTLVNVTDRAFTVIGRRGSSAGLVRATIGALAFGVFPLVTRTSLGTRLGGYIGQYR
IRYRYTANQTERERDPAVGLRLPPVADNQRALDTMTWQLHAYGAGTIVRPDVPEWIDGPREFGADPRGRLRSDRLYLVRP
DQFVAASIPLRDNVVDEAHLRAALATHQILHAGP

Specific function: Dechlorination of pentachlorophenol to tetrachlorohydroquinone. Removes also hydrogen and nitro, amino, and cyano groups from benzene ring at the para position in relation to the hydroxyl of phenol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=516, Percent_Identity=27.1317829457364, Blast_Score=109, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: =1.14.13.50 [H]

Molecular weight: Translated: 55699; Mature: 55568

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPAYRVDDLPVTDTDVLIVGAGPTGLMAALALHRRGVPAVLVDRKAGPTRESRALAVQA
CCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEH
RTMEVYDQLGLADTVLANSNSALRIQVGGSSRGIGPNLAKLQDGHTRFPGVHIFEQSRNE
HHHHHHHHCCCHHEEEECCCCEEEEEECCCCCCCCCCHHHHCCCCCCCCCEEEEECCCCC
DMLAGALTAGGGDIRWRHRLVDLQDRTAAPDGRVEALLEGPDGTLLRVRARWCIGADGAA
CEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCEEEEEECCCCCEEEEEEEEEECCCHHH
SAVRRALNLPFEGRTDEATFWVADVRAVRGVPDDAVNIRPGKRTFAAVFPLGPGGHVRLL
HHHHHHHCCCCCCCCCCCEEEEEEHHHHCCCCCCCEECCCCCEEEEEEEECCCCCCEEEE
GLAAKDTITQDEALATVAAEFGLTHGPVEWFSTYRVHHRVSARFRVGSIFLAGDAAHVHS
EEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHEEEEEEEEEEEEEEECCHHHHCC
PVGGQGMNTGLQDAHNLAMLLADVAQNRVDGRALDRYERERRPVALTLVNVTDRAFTVIG
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEEECCCEEEEEE
RRGSSAGLVRATIGALAFGVFPLVTRTSLGTRLGGYIGQYRIRYRYTANQTERERDPAVG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCCCCCCC
LRLPPVADNQRALDTMTWQLHAYGAGTIVRPDVPEWIDGPREFGADPRGRLRSDRLYLVR
EECCCCCCCCHHHEEEEEEEEEECCCEEECCCCHHHCCCHHHHCCCCCCCCCCCCEEEEC
PDQFVAASIPLRDNVVDEAHLRAALATHQILHAGP
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SPAYRVDDLPVTDTDVLIVGAGPTGLMAALALHRRGVPAVLVDRKAGPTRESRALAVQA
CCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEEH
RTMEVYDQLGLADTVLANSNSALRIQVGGSSRGIGPNLAKLQDGHTRFPGVHIFEQSRNE
HHHHHHHHCCCHHEEEECCCCEEEEEECCCCCCCCCCHHHHCCCCCCCCCEEEEECCCCC
DMLAGALTAGGGDIRWRHRLVDLQDRTAAPDGRVEALLEGPDGTLLRVRARWCIGADGAA
CEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCEEEEEECCCCCEEEEEEEEEECCCHHH
SAVRRALNLPFEGRTDEATFWVADVRAVRGVPDDAVNIRPGKRTFAAVFPLGPGGHVRLL
HHHHHHHCCCCCCCCCCCEEEEEEHHHHCCCCCCCEECCCCCEEEEEEEECCCCCCEEEE
GLAAKDTITQDEALATVAAEFGLTHGPVEWFSTYRVHHRVSARFRVGSIFLAGDAAHVHS
EEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHEEEEEEEEEEEEEEECCHHHHCC
PVGGQGMNTGLQDAHNLAMLLADVAQNRVDGRALDRYERERRPVALTLVNVTDRAFTVIG
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEEECCCEEEEEE
RRGSSAGLVRATIGALAFGVFPLVTRTSLGTRLGGYIGQYRIRYRYTANQTERERDPAVG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCCCCCCC
LRLPPVADNQRALDTMTWQLHAYGAGTIVRPDVPEWIDGPREFGADPRGRLRSDRLYLVR
EECCCCCCCCHHHEEEEEEEEEECCCEEECCCCHHHCCCHHHHCCCCCCCCCCCCEEEEC
PDQFVAASIPLRDNVVDEAHLRAALATHQILHAGP
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7678243; 12169590 [H]