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The map label for this gene is nemA [C]

Identifier: 119871409

GI number: 119871409

Start: 5619372

End: 5620463

Strand: Direct

Name: nemA [C]

Synonym: Mkms_5385

Alternate gene names: 119871409

Gene position: 5619372-5620463 (Clockwise)

Preceding gene: 119871406

Following gene: 119871410

Centisome position: 97.95

GC content: 71.06

Gene sequence:

>1092_bases
GTGGCTATCGACGACCTTTTTCAACCGCTGACCGTCCGCTCGTTGACCGTGCCCAACCGGTTCGCGATGGCGCCGATGAC
CCGGCAGGCGTCGCCGGACGGTGTCCCGGGTTCCGACGTGGCGGAGTACTACCGTCGCCGCGCCGCCGGCGGGGTGGGGC
TGATCATCACCGAAGGGGTCCGGCTGCCCGACCCGGCTGCGGGTTACCCCTCGTCCATTCCGACGATCGCCGGCGACGAT
GTCCTCGACGGCTGGTCGCGTGTCGTCCGCGCCGTGCACGCCGCGGGCGGAACGATCGCCGCGCAGCTGTGGCACCAGGG
CGCCGAGCGCAGTGACGCCGACGGTGTCGCCCCGGTCAGCCCGTCGGGCGTCGACGGGCTCGGCAGGCCGAAGGGCCGGG
CGCTCGCGACCCACGAACTCGACGACATCGCCGCGCTGTTCGCGCAGAGTGCCCGCACCGCACGCGAGGTCGGCTTCGAC
GCCGTCGAACTCCACGGCGCCCACGGCTATCTTCTCGACGAATTCTTCTGGGAGCGTACGAATTCCCGCGCCGACGGATA
TGGCGGCGACCTCGCCGCGCGCACCCGGTTCCCCGCCGAGGTGGTGGCCGCGGTGCGCGCCGCCGTCGGACCGGACTATC
CGATCATCTTCCGCTTCTCGCAGTGGAAGGGCCTGGACTACACCGCGTCCATCGCCGACGACCCCACGCAGCTGCAGGAA
CTGCTCGCTCCCCTGATCGAGGCGGGGGTCGACGTCCTCCACCCCTCCACGCGCAGGCATTACGTGCCCGCCTTCCCCGA
TCACGGCGAGCTCAGTCTCGCGGGTTGGACCAAGAAGGTGACCGGGCTACCGGTGATCGCCGTGGGCTCCGTCGGGCTCG
AGACCCAGTTCCGCAGCGAGAAGCAGGGGCAGGTGATCCAGCCCGCGCCCGTCGAGCGTCTGGTCGAGCAGTTCGAGGCC
GGCGAATTCGACATCGTGGCGATCGGACGCGCGCTGCTGGCCGACCCGGCGTGGGTGAACCGGCTGCGTGACGGTGAACT
CGGCGGATTCGCCGGCTACGATCCCGCGGCCGCGCTCGCCACGCTCGCCTGA

Upstream 100 bases:

>100_bases
CGAGGGTGAGGTCGACAAACCCTCCGAGGGGTGACGCTCGCGCGGCGCATGTGACCCACGACGCGGAATCACCGCCACCG
TCCCCGCGTTGCACGCCTTT

Downstream 100 bases:

>100_bases
TCGGCCCGGGCCACCGCGACTTCGGGCTACGGTGACCGTATGTCGCCCGCCTACCGCGTCGACGACCTCCCGGTCACCGA
CACCGACGTCCTGATCGTCG

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MAIDDLFQPLTVRSLTVPNRFAMAPMTRQASPDGVPGSDVAEYYRRRAAGGVGLIITEGVRLPDPAAGYPSSIPTIAGDD
VLDGWSRVVRAVHAAGGTIAAQLWHQGAERSDADGVAPVSPSGVDGLGRPKGRALATHELDDIAALFAQSARTAREVGFD
AVELHGAHGYLLDEFFWERTNSRADGYGGDLAARTRFPAEVVAAVRAAVGPDYPIIFRFSQWKGLDYTASIADDPTQLQE
LLAPLIEAGVDVLHPSTRRHYVPAFPDHGELSLAGWTKKVTGLPVIAVGSVGLETQFRSEKQGQVIQPAPVERLVEQFEA
GEFDIVAIGRALLADPAWVNRLRDGELGGFAGYDPAAALATLA

Sequences:

>Translated_363_residues
MAIDDLFQPLTVRSLTVPNRFAMAPMTRQASPDGVPGSDVAEYYRRRAAGGVGLIITEGVRLPDPAAGYPSSIPTIAGDD
VLDGWSRVVRAVHAAGGTIAAQLWHQGAERSDADGVAPVSPSGVDGLGRPKGRALATHELDDIAALFAQSARTAREVGFD
AVELHGAHGYLLDEFFWERTNSRADGYGGDLAARTRFPAEVVAAVRAAVGPDYPIIFRFSQWKGLDYTASIADDPTQLQE
LLAPLIEAGVDVLHPSTRRHYVPAFPDHGELSLAGWTKKVTGLPVIAVGSVGLETQFRSEKQGQVIQPAPVERLVEQFEA
GEFDIVAIGRALLADPAWVNRLRDGELGGFAGYDPAAALATLA
>Mature_362_residues
AIDDLFQPLTVRSLTVPNRFAMAPMTRQASPDGVPGSDVAEYYRRRAAGGVGLIITEGVRLPDPAAGYPSSIPTIAGDDV
LDGWSRVVRAVHAAGGTIAAQLWHQGAERSDADGVAPVSPSGVDGLGRPKGRALATHELDDIAALFAQSARTAREVGFDA
VELHGAHGYLLDEFFWERTNSRADGYGGDLAARTRFPAEVVAAVRAAVGPDYPIIFRFSQWKGLDYTASIADDPTQLQEL
LAPLIEAGVDVLHPSTRRHYVPAFPDHGELSLAGWTKKVTGLPVIAVGSVGLETQFRSEKQGQVIQPAPVERLVEQFEAG
EFDIVAIGRALLADPAWVNRLRDGELGGFAGYDPAAALATLA

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=374, Percent_Identity=33.4224598930481, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1789463, Length=373, Percent_Identity=29.4906166219839, Blast_Score=117, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17564188, Length=217, Percent_Identity=32.258064516129, Blast_Score=85, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17540738, Length=214, Percent_Identity=29.9065420560748, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17559804, Length=217, Percent_Identity=31.3364055299539, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17565138, Length=215, Percent_Identity=29.7674418604651, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17559802, Length=213, Percent_Identity=31.4553990610329, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI72001454, Length=219, Percent_Identity=30.1369863013699, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6321973, Length=225, Percent_Identity=32, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6325086, Length=225, Percent_Identity=31.5555555555556, Blast_Score=94, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 38607; Mature: 38476

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIDDLFQPLTVRSLTVPNRFAMAPMTRQASPDGVPGSDVAEYYRRRAAGGVGLIITEGV
CCHHHHHCCHHHEEECCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCC
RLPDPAAGYPSSIPTIAGDDVLDGWSRVVRAVHAAGGTIAAQLWHQGAERSDADGVAPVS
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCC
PSGVDGLGRPKGRALATHELDDIAALFAQSARTAREVGFDAVELHGAHGYLLDEFFWERT
CCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHC
NSRADGYGGDLAARTRFPAEVVAAVRAAVGPDYPIIFRFSQWKGLDYTASIADDPTQLQE
CCCCCCCCCCCHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCEEECCCCCHHHHHH
LLAPLIEAGVDVLHPSTRRHYVPAFPDHGELSLAGWTKKVTGLPVIAVGSVGLETQFRSE
HHHHHHHCCHHHCCCCCCCCCCCCCCCCCCEEECCCCHHHCCCCEEEECCCCCCHHHCCC
KQGQVIQPAPVERLVEQFEAGEFDIVAIGRALLADPAWVNRLRDGELGGFAGYDPAAALA
CCCCCCCCCHHHHHHHHHCCCCEEEEEHHHHHHCCHHHHHHCCCCCCCCCCCCCHHHHHH
TLA
HCC
>Mature Secondary Structure 
AIDDLFQPLTVRSLTVPNRFAMAPMTRQASPDGVPGSDVAEYYRRRAAGGVGLIITEGV
CHHHHHCCHHHEEECCCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCC
RLPDPAAGYPSSIPTIAGDDVLDGWSRVVRAVHAAGGTIAAQLWHQGAERSDADGVAPVS
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCC
PSGVDGLGRPKGRALATHELDDIAALFAQSARTAREVGFDAVELHGAHGYLLDEFFWERT
CCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHC
NSRADGYGGDLAARTRFPAEVVAAVRAAVGPDYPIIFRFSQWKGLDYTASIADDPTQLQE
CCCCCCCCCCCHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCEEECCCCCHHHHHH
LLAPLIEAGVDVLHPSTRRHYVPAFPDHGELSLAGWTKKVTGLPVIAVGSVGLETQFRSE
HHHHHHHCCHHHCCCCCCCCCCCCCCCCCCEEECCCCHHHCCCCEEEECCCCCCHHHCCC
KQGQVIQPAPVERLVEQFEAGEFDIVAIGRALLADPAWVNRLRDGELGGFAGYDPAAALA
CCCCCCCCCHHHHHHHHHCCCCEEEEEHHHHHHCCHHHHHHCCCCCCCCCCCCCHHHHHH
TLA
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]