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The map label for this gene is nylA [H]

Identifier: 119871406

GI number: 119871406

Start: 5615998

End: 5617467

Strand: Direct

Name: nylA [H]

Synonym: Mkms_5382

Alternate gene names: 119871406

Gene position: 5615998-5617467 (Clockwise)

Preceding gene: 119871405

Following gene: 119871409

Centisome position: 97.89

GC content: 70.0

Gene sequence:

>1470_bases
GTGAACTTCGAGGAATACCGAACACACGACGCGGTCGGGCTGGCCAAGTTGGTCGCCGACAAGCAGGTGACCGCGGCCGA
GCTGCTGGACCTGGCGAAGCAGCGGGCGGCCGCGGTGAATCCACGGATCAACGCGATCGTGCGCGACGTCCCCGGCTCGC
CGTCACCCGACTCCGGTGGCCCGTTCGCCGGTGTGCCGTTCCTCATCAAGGACCTCGCGCAGGACTACGCGGGGCTGCCC
ACCTCGGCGGGGTCCCGCGCGCTGATGTCGACACCGGTCGCCGAACACGCGACCGTCGTGCAGCGGTGGCTCGAAGCCGG
TCTGGCGATCTTCGGCAAGACCAACACCCCGGAGTTCGGGGCCAAGGGCATCACCGAGCCCGACGCCTGGGGTCCGGCGC
GCAACCCGTGGGATCTGTCGCGGACGCCGGGCGGATCCTCGGGTGGTTCGGCCGCCGCGGTCGCCGCCGGAATCGTGCCG
TGCGCCGGCGCCAACGACGGTGGCGGCTCGATCCGCATCCCCGCCGCCTGCTGTGGGCTGGTCGGGCTCAAACCGGGTCG
GGGACTGACACCGTCGGGCCCGGCGGTCGGTGAGTCCATGCACGGAGCGGCCGTACAGGGTGTCGTATCCCGGACGGTGC
GGGACACGGCCGCGATGCTCGATGTCCTCAAGGGCGGCGAGGCCTGCGGTCCGTATGTGCCCGCCGTACCGACCGACTCG
TTCATGTCGTGTGTGGGACAGGAGCCGGGAACTCTGCGCATCGGGTTGCGGGTCCCGTCGGCGATCAACCCGACACCGCA
CCCGGAGGCGCGGGCCGCCGTAGAGAGGACCGTCCTGGCGTTGACCGACCTGGGCCACCACGTCGAGGAGTTGCCCGTCG
CACCGTTCGACGACGCCGCACTCGCCCGCGACTTCCTGCTGACATGGTTCGTCTACACCGCGTGGGAACTCGATGACGCC
AAACGTGTTTCGGGCGCCGGCGACGACGCGTTCGAGCGGGACACGCTGATCCTGGCGGCGATCGGCCGGGCGACGAGCAG
TGTGGACTACATCGACGCCGTTCAGCGCCGCCACGAGCACACGCGCCGGCTGACCACCTTCTTCGAGTCCTACGACCTGC
TGTTGACGCCGACGCTGGCGACCCCACCGCCACGGATCGGTGAGTTCGACCTGCCTGCCGCCCTGCAGCGCGCGTCCGAC
GTACTGATCAAGACGCGCACGGCGCGGTTACTGCGGCACACGAACATCGTCGACGACATGGTGGACAAGAACCTGTCCTG
GGTGCCCTACACCCAGCTCGCGAATCTGACTGGCCGACCGGCGATCTCGCTGCCGTTGCACTGGACGGCGGACGGTCTGC
CGCTGGGGGTGCAGTTCGTCGCGCCGCTCGCGGGTGAGGCGCTGCTGATCCGCTTGGCCGCCCAACTCGAGCAGGCGCTG
CCCTGGGCGGATCGCGTCGCACCGGTCTAG

Upstream 100 bases:

>100_bases
ACTGTCCGCGGGCAGCGCTGTCGCGCGAGGACTAGCCTCCGGGGGCGCCGGGACGCGGGAAGCCTCCCCGCGCATCGGCC
GTGGGACCTACGGTCATGGG

Downstream 100 bases:

>100_bases
ATGTCACCCGCGAGGCGCTGACGGAGGGCGGCCGCGCGGACCGTGAGATGGTTCCGCTCGGCGAGGTTCTGCGCCTGATT
CGCGGCCTGCACGTAAAGCT

Product: amidase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MNFEEYRTHDAVGLAKLVADKQVTAAELLDLAKQRAAAVNPRINAIVRDVPGSPSPDSGGPFAGVPFLIKDLAQDYAGLP
TSAGSRALMSTPVAEHATVVQRWLEAGLAIFGKTNTPEFGAKGITEPDAWGPARNPWDLSRTPGGSSGGSAAAVAAGIVP
CAGANDGGGSIRIPAACCGLVGLKPGRGLTPSGPAVGESMHGAAVQGVVSRTVRDTAAMLDVLKGGEACGPYVPAVPTDS
FMSCVGQEPGTLRIGLRVPSAINPTPHPEARAAVERTVLALTDLGHHVEELPVAPFDDAALARDFLLTWFVYTAWELDDA
KRVSGAGDDAFERDTLILAAIGRATSSVDYIDAVQRRHEHTRRLTTFFESYDLLLTPTLATPPPRIGEFDLPAALQRASD
VLIKTRTARLLRHTNIVDDMVDKNLSWVPYTQLANLTGRPAISLPLHWTADGLPLGVQFVAPLAGEALLIRLAAQLEQAL
PWADRVAPV

Sequences:

>Translated_489_residues
MNFEEYRTHDAVGLAKLVADKQVTAAELLDLAKQRAAAVNPRINAIVRDVPGSPSPDSGGPFAGVPFLIKDLAQDYAGLP
TSAGSRALMSTPVAEHATVVQRWLEAGLAIFGKTNTPEFGAKGITEPDAWGPARNPWDLSRTPGGSSGGSAAAVAAGIVP
CAGANDGGGSIRIPAACCGLVGLKPGRGLTPSGPAVGESMHGAAVQGVVSRTVRDTAAMLDVLKGGEACGPYVPAVPTDS
FMSCVGQEPGTLRIGLRVPSAINPTPHPEARAAVERTVLALTDLGHHVEELPVAPFDDAALARDFLLTWFVYTAWELDDA
KRVSGAGDDAFERDTLILAAIGRATSSVDYIDAVQRRHEHTRRLTTFFESYDLLLTPTLATPPPRIGEFDLPAALQRASD
VLIKTRTARLLRHTNIVDDMVDKNLSWVPYTQLANLTGRPAISLPLHWTADGLPLGVQFVAPLAGEALLIRLAAQLEQAL
PWADRVAPV
>Mature_489_residues
MNFEEYRTHDAVGLAKLVADKQVTAAELLDLAKQRAAAVNPRINAIVRDVPGSPSPDSGGPFAGVPFLIKDLAQDYAGLP
TSAGSRALMSTPVAEHATVVQRWLEAGLAIFGKTNTPEFGAKGITEPDAWGPARNPWDLSRTPGGSSGGSAAAVAAGIVP
CAGANDGGGSIRIPAACCGLVGLKPGRGLTPSGPAVGESMHGAAVQGVVSRTVRDTAAMLDVLKGGEACGPYVPAVPTDS
FMSCVGQEPGTLRIGLRVPSAINPTPHPEARAAVERTVLALTDLGHHVEELPVAPFDDAALARDFLLTWFVYTAWELDDA
KRVSGAGDDAFERDTLILAAIGRATSSVDYIDAVQRRHEHTRRLTTFFESYDLLLTPTLATPPPRIGEFDLPAALQRASD
VLIKTRTARLLRHTNIVDDMVDKNLSWVPYTQLANLTGRPAISLPLHWTADGLPLGVQFVAPLAGEALLIRLAAQLEQAL
PWADRVAPV

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=512, Percent_Identity=23.2421875, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI222831590, Length=188, Percent_Identity=32.9787234042553, Blast_Score=76, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17556264, Length=319, Percent_Identity=29.7805642633229, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17556276, Length=310, Percent_Identity=30.3225806451613, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17556278, Length=310, Percent_Identity=30.3225806451613, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17543272, Length=484, Percent_Identity=25.4132231404959, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17537465, Length=238, Percent_Identity=28.1512605042017, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71990152, Length=238, Percent_Identity=29.4117647058824, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17538252, Length=243, Percent_Identity=28.8065843621399, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=428, Percent_Identity=25.7009345794392, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24644968, Length=506, Percent_Identity=24.1106719367589, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI45550774, Length=195, Percent_Identity=33.8461538461538, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648435, Length=195, Percent_Identity=33.8461538461538, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24648437, Length=195, Percent_Identity=33.8461538461538, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21356731, Length=238, Percent_Identity=31.5126050420168, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24648113, Length=496, Percent_Identity=30.4435483870968, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI161078093, Length=518, Percent_Identity=23.9382239382239, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24648441, Length=126, Percent_Identity=38.0952380952381, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648439, Length=126, Percent_Identity=38.0952380952381, Blast_Score=71, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 51521; Mature: 51521

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFEEYRTHDAVGLAKLVADKQVTAAELLDLAKQRAAAVNPRINAIVRDVPGSPSPDSGG
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHEECCCCCCCCCCCC
PFAGVPFLIKDLAQDYAGLPTSAGSRALMSTPVAEHATVVQRWLEAGLAIFGKTNTPEFG
CCCCHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCEEEECCCCCCCCC
AKGITEPDAWGPARNPWDLSRTPGGSSGGSAAAVAAGIVPCAGANDGGGSIRIPAACCGL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCEEECHHHHHHH
VGLKPGRGLTPSGPAVGESMHGAAVQGVVSRTVRDTAAMLDVLKGGEACGPYVPAVPTDS
HCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
FMSCVGQEPGTLRIGLRVPSAINPTPHPEARAAVERTVLALTDLGHHVEELPVAPFDDAA
HHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LARDFLLTWFVYTAWELDDAKRVSGAGDDAFERDTLILAAIGRATSSVDYIDAVQRRHEH
HHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHCCEEEEECCCCCCCHHHHHHHHHHHHH
TRRLTTFFESYDLLLTPTLATPPPRIGEFDLPAALQRASDVLIKTRTARLLRHTNIVDDM
HHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDKNLSWVPYTQLANLTGRPAISLPLHWTADGLPLGVQFVAPLAGEALLIRLAAQLEQAL
HHCCCCCCCHHHHHCCCCCCEEEECEEECCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHC
PWADRVAPV
CCHHHCCCC
>Mature Secondary Structure
MNFEEYRTHDAVGLAKLVADKQVTAAELLDLAKQRAAAVNPRINAIVRDVPGSPSPDSGG
CCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHEECCCCCCCCCCCC
PFAGVPFLIKDLAQDYAGLPTSAGSRALMSTPVAEHATVVQRWLEAGLAIFGKTNTPEFG
CCCCHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCEEEECCCCCCCCC
AKGITEPDAWGPARNPWDLSRTPGGSSGGSAAAVAAGIVPCAGANDGGGSIRIPAACCGL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCEEECHHHHHHH
VGLKPGRGLTPSGPAVGESMHGAAVQGVVSRTVRDTAAMLDVLKGGEACGPYVPAVPTDS
HCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
FMSCVGQEPGTLRIGLRVPSAINPTPHPEARAAVERTVLALTDLGHHVEELPVAPFDDAA
HHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
LARDFLLTWFVYTAWELDDAKRVSGAGDDAFERDTLILAAIGRATSSVDYIDAVQRRHEH
HHHHHHHHHHHHHHHCCCCHHHHCCCCCCCHHHCCEEEEECCCCCCCHHHHHHHHHHHHH
TRRLTTFFESYDLLLTPTLATPPPRIGEFDLPAALQRASDVLIKTRTARLLRHTNIVDDM
HHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDKNLSWVPYTQLANLTGRPAISLPLHWTADGLPLGVQFVAPLAGEALLIRLAAQLEQAL
HHCCCCCCCHHHHHCCCCCCEEEECEEECCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHC
PWADRVAPV
CCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]