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The map label for this gene is caiD [H]

Identifier: 119870797

GI number: 119870797

Start: 5000859

End: 5001617

Strand: Direct

Name: caiD [H]

Synonym: Mkms_4769

Alternate gene names: 119870797

Gene position: 5000859-5001617 (Clockwise)

Preceding gene: 119870794

Following gene: 119870798

Centisome position: 87.17

GC content: 66.53

Gene sequence:

>759_bases
ATGCCGATCACCACAAAGACCGTGGAACCGGGCATCGTCTCGGTCACGGTCGACTACCCGCCCGTCAACGCGATCCCGTC
GCGGGGCTGGTTCGAACTCGGCGACACGATCACCGCGGCGGGCCGGGACCGCAGCACCCACGTGGTGATCCTGCGCGCCG
AGGGCCGCGGCTTCAACGCCGGTGTCGACATCAAGGAGATGCAGAACACCGAGGGCTTCACGGCGCTCATCGACGCCAAT
CGCGGTTGTTTCCACGCCTTCCGCGCGGTGTACGAATGCGAGGTTCCCGTCATCGCCGCGGTCAACGGCTTCTGCGTCGG
TGGCGGCATCGGTCTGGTCGGCAACGCCGACGTGATCGTCGCCTCCGACGATGCGAAGTTCGGCCTGCCCGAGGTCGAGC
GTGGCGCACTGGGTGCGGCCACCCACCTTTCGCGCCTGGTGCCGCAACACATGATGCGCCGCATGTTCTTCACCGCGGCG
ACCGTGAGCGCCGAGACCCTGCACCATTTCGGTTCGGTACACGAGGTGGTGCCGCGCGAGGACCTGGACGAGTCAGCGCT
GCGGGTGGCCCGCGACATCGCGAGCAAGGACACCCGCGTGATCCGCGCCGCCAAGGAAGCCCTCAACTTCATCGATGTCC
AGCCGGTCAACGCGAGATACCGCATGGAGCAGGGCTTCACATTCGAGCTGAACCTGGCAGGAGTGTCCGACGAGCACCGC
GACGCCTTCGCCGGGACATCGAAGGGCGAGACGAAATGA

Upstream 100 bases:

>100_bases
CCACTCGGCCATCCAGTCCCAGTTTGATTCCAGCGGCGTCGCTCACTGTGCTAGCGTACCAAGCAAGTGCTTGCTTAGGT
AGAGACCCCCAGGAGTTTGC

Downstream 100 bases:

>100_bases
CCGATAAGACAACGAGTCTCGACGAGGCCGTCGCGTCCGTCGAGAGCGGGATGACCATCGGCATCGGCGGTTGGGGCTCA
CGCCGCAAGCCGATGGCGCT

Product: enoyl-CoA hydratase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: Crotonobetainyl-CoA hydratase [H]

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MPITTKTVEPGIVSVTVDYPPVNAIPSRGWFELGDTITAAGRDRSTHVVILRAEGRGFNAGVDIKEMQNTEGFTALIDAN
RGCFHAFRAVYECEVPVIAAVNGFCVGGGIGLVGNADVIVASDDAKFGLPEVERGALGAATHLSRLVPQHMMRRMFFTAA
TVSAETLHHFGSVHEVVPREDLDESALRVARDIASKDTRVIRAAKEALNFIDVQPVNARYRMEQGFTFELNLAGVSDEHR
DAFAGTSKGETK

Sequences:

>Translated_252_residues
MPITTKTVEPGIVSVTVDYPPVNAIPSRGWFELGDTITAAGRDRSTHVVILRAEGRGFNAGVDIKEMQNTEGFTALIDAN
RGCFHAFRAVYECEVPVIAAVNGFCVGGGIGLVGNADVIVASDDAKFGLPEVERGALGAATHLSRLVPQHMMRRMFFTAA
TVSAETLHHFGSVHEVVPREDLDESALRVARDIASKDTRVIRAAKEALNFIDVQPVNARYRMEQGFTFELNLAGVSDEHR
DAFAGTSKGETK
>Mature_251_residues
PITTKTVEPGIVSVTVDYPPVNAIPSRGWFELGDTITAAGRDRSTHVVILRAEGRGFNAGVDIKEMQNTEGFTALIDANR
GCFHAFRAVYECEVPVIAAVNGFCVGGGIGLVGNADVIVASDDAKFGLPEVERGALGAATHLSRLVPQHMMRRMFFTAAT
VSAETLHHFGSVHEVVPREDLDESALRVARDIASKDTRVIRAAKEALNFIDVQPVNARYRMEQGFTFELNLAGVSDEHRD
AFAGTSKGETK

Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=186, Percent_Identity=31.7204301075269, Blast_Score=91, Evalue=7e-19,
Organism=Homo sapiens, GI31542718, Length=160, Percent_Identity=34.375, Blast_Score=91, Evalue=8e-19,
Organism=Homo sapiens, GI70995211, Length=216, Percent_Identity=27.7777777777778, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI221142681, Length=214, Percent_Identity=32.2429906542056, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1787659, Length=233, Percent_Identity=30.0429184549356, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788597, Length=209, Percent_Identity=28.7081339712919, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788682, Length=149, Percent_Identity=31.5436241610738, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1787660, Length=249, Percent_Identity=25.3012048192771, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17554946, Length=225, Percent_Identity=30.2222222222222, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI25145438, Length=214, Percent_Identity=29.4392523364486, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17534483, Length=246, Percent_Identity=29.2682926829268, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17536985, Length=230, Percent_Identity=26.5217391304348, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17540714, Length=186, Percent_Identity=30.6451612903226, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17540306, Length=185, Percent_Identity=28.1081081081081, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI20129971, Length=222, Percent_Identity=29.7297297297297, Blast_Score=98, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24653477, Length=222, Percent_Identity=29.7297297297297, Blast_Score=98, Evalue=4e-21,
Organism=Drosophila melanogaster, GI19920382, Length=219, Percent_Identity=30.1369863013699, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24653139, Length=210, Percent_Identity=28.5714285714286, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19922422, Length=199, Percent_Identity=24.6231155778894, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022852
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 27227; Mature: 27096

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPITTKTVEPGIVSVTVDYPPVNAIPSRGWFELGDTITAAGRDRSTHVVILRAEGRGFNA
CCCCCCCCCCCEEEEEECCCCCCCCCCCCCEECCCEEEECCCCCCEEEEEEEECCCCCCC
GVDIKEMQNTEGFTALIDANRGCFHAFRAVYECEVPVIAAVNGFCVGGGIGLVGNADVIV
CCCHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCEEEECCEEEECCCEEEE
ASDDAKFGLPEVERGALGAATHLSRLVPQHMMRRMFFTAATVSAETLHHFGSVHEVVPRE
ECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCC
DLDESALRVARDIASKDTRVIRAAKEALNFIDVQPVNARYRMEQGFTFELNLAGVSDEHR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEECCCCEEEEEECCCCCCHH
DAFAGTSKGETK
HHHCCCCCCCCC
>Mature Secondary Structure 
PITTKTVEPGIVSVTVDYPPVNAIPSRGWFELGDTITAAGRDRSTHVVILRAEGRGFNA
CCCCCCCCCCEEEEEECCCCCCCCCCCCCEECCCEEEECCCCCCEEEEEEEECCCCCCC
GVDIKEMQNTEGFTALIDANRGCFHAFRAVYECEVPVIAAVNGFCVGGGIGLVGNADVIV
CCCHHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCEEEEECCEEEECCEEEECCCEEEE
ASDDAKFGLPEVERGALGAATHLSRLVPQHMMRRMFFTAATVSAETLHHFGSVHEVVPRE
ECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCC
DLDESALRVARDIASKDTRVIRAAKEALNFIDVQPVNARYRMEQGFTFELNLAGVSDEHR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEEECCCCEEEEEECCCCCCHH
DAFAGTSKGETK
HHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA