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The map label for this gene is paaF [H]

Identifier: 119870745

GI number: 119870745

Start: 4941072

End: 4941881

Strand: Direct

Name: paaF [H]

Synonym: Mkms_4716

Alternate gene names: 119870745

Gene position: 4941072-4941881 (Clockwise)

Preceding gene: 119870741

Following gene: 119870746

Centisome position: 86.12

GC content: 66.05

Gene sequence:

>810_bases
GTGAGCGACGCGCAAGTCACCGAAAAGCAACCCGACGCACTCGTTGAGCAGCGCGGACACACGCTGGTCGTGACCTTGAA
CCGGCCGGAGTCGCGAAACGCGCTTTCGACCGAGATGCTCTCGATCATGGTCGACGCGTGGGACCGGGTCGACAACGATC
CGGAGATCCGCTCGTGCATCCTCACCGGCGCCGGTGGCTACTTCTGTGCCGGGATGGACCTCAAGGCCGCGACGGCCAAA
CCGCCGGGCGACTCGTTCAAGGACGGCAGCTACGACCCGTCGCGCATCGACGGTCTGCTCAAGGGCCGCCGGCTGACCAA
ACCACTGATCGCCGCCGTCGAGGGTCCGGCGATCGCCGGCGGTACGGAGATCCTGCAGGGCACCGATATCCGGGTGGCCG
GCGAGAGCGCGAAGTTCGGGATCTCCGAGGCCAAGTGGAGCCTGTACCCGATGGGTGGCTCGGCGGTGCGCCTGGTTCGC
CAGATCCCGTACACGATCGCGTGCGACCTGCTGCTGACCGGACGGCACATCACCGCCGCCGAGGCCAAGGAGTACGGGCT
GATCGGTTACGTCGTCCCCGACGGCACGGCGCTGGACAAGGCACTCGAGATCGCCGAGGTGATCAACAACAACGGCCCGC
TGGCCGTGCAGGCGATCCTGCGGTCCATCCGCGAGACGGAAGGCCTGCACGAGAACGAGGCGTTCAAGATCGACACCAAG
ATCGGCATCGAGGTCTTCCTCTCCGAAGACGCCAAGGAGGGCCCGCGGGCGTTCAAGGAGAAGCGGGCGCCGAACTTCAA
GATGAAGTAG

Upstream 100 bases:

>100_bases
GCTCGGCAAGATCGGCGATGTTCAGGGCCACGGACCCAAACTAGAACGTGTTACATTTCCTGACAAGTCTGTGGCATCGA
CGCTGGAAGGCGGACTGTCT

Downstream 100 bases:

>100_bases
GCCCTCGCGCCGAACGTGCGCTGAGTGCGAAAACCTGGGCATTTTCTCGCAGTGAGCGCACGTTCGATGCCGTGTCGGTG
CCCGTCGGCACACTCCCCGC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MSDAQVTEKQPDALVEQRGHTLVVTLNRPESRNALSTEMLSIMVDAWDRVDNDPEIRSCILTGAGGYFCAGMDLKAATAK
PPGDSFKDGSYDPSRIDGLLKGRRLTKPLIAAVEGPAIAGGTEILQGTDIRVAGESAKFGISEAKWSLYPMGGSAVRLVR
QIPYTIACDLLLTGRHITAAEAKEYGLIGYVVPDGTALDKALEIAEVINNNGPLAVQAILRSIRETEGLHENEAFKIDTK
IGIEVFLSEDAKEGPRAFKEKRAPNFKMK

Sequences:

>Translated_269_residues
MSDAQVTEKQPDALVEQRGHTLVVTLNRPESRNALSTEMLSIMVDAWDRVDNDPEIRSCILTGAGGYFCAGMDLKAATAK
PPGDSFKDGSYDPSRIDGLLKGRRLTKPLIAAVEGPAIAGGTEILQGTDIRVAGESAKFGISEAKWSLYPMGGSAVRLVR
QIPYTIACDLLLTGRHITAAEAKEYGLIGYVVPDGTALDKALEIAEVINNNGPLAVQAILRSIRETEGLHENEAFKIDTK
IGIEVFLSEDAKEGPRAFKEKRAPNFKMK
>Mature_268_residues
SDAQVTEKQPDALVEQRGHTLVVTLNRPESRNALSTEMLSIMVDAWDRVDNDPEIRSCILTGAGGYFCAGMDLKAATAKP
PGDSFKDGSYDPSRIDGLLKGRRLTKPLIAAVEGPAIAGGTEILQGTDIRVAGESAKFGISEAKWSLYPMGGSAVRLVRQ
IPYTIACDLLLTGRHITAAEAKEYGLIGYVVPDGTALDKALEIAEVINNNGPLAVQAILRSIRETEGLHENEAFKIDTKI
GIEVFLSEDAKEGPRAFKEKRAPNFKMK

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=257, Percent_Identity=33.0739299610895, Blast_Score=128, Evalue=5e-30,
Organism=Homo sapiens, GI4502327, Length=258, Percent_Identity=32.9457364341085, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI70995211, Length=216, Percent_Identity=33.3333333333333, Blast_Score=99, Evalue=3e-21,
Organism=Homo sapiens, GI31542718, Length=242, Percent_Identity=27.2727272727273, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI45643119, Length=229, Percent_Identity=29.6943231441048, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI260275230, Length=229, Percent_Identity=29.6943231441048, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI260274832, Length=225, Percent_Identity=30.2222222222222, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI20127408, Length=186, Percent_Identity=31.7204301075269, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI4757968, Length=196, Percent_Identity=28.0612244897959, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI49355787, Length=196, Percent_Identity=28.0612244897959, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI116235487, Length=218, Percent_Identity=26.1467889908257, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI68989263, Length=185, Percent_Identity=28.6486486486486, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI37594469, Length=195, Percent_Identity=28.7179487179487, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI37594471, Length=195, Percent_Identity=28.7179487179487, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI221136756, Length=222, Percent_Identity=25.6756756756757, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI221136753, Length=222, Percent_Identity=25.6756756756757, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI221307494, Length=222, Percent_Identity=25.6756756756757, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1787659, Length=254, Percent_Identity=32.6771653543307, Blast_Score=149, Evalue=1e-37,
Organism=Escherichia coli, GI221142681, Length=257, Percent_Identity=36.1867704280156, Blast_Score=141, Evalue=4e-35,
Organism=Escherichia coli, GI1787660, Length=257, Percent_Identity=33.4630350194552, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI87082183, Length=257, Percent_Identity=29.9610894941634, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI1788597, Length=252, Percent_Identity=27.7777777777778, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1788682, Length=192, Percent_Identity=28.125, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17554946, Length=273, Percent_Identity=31.1355311355311, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI25145438, Length=244, Percent_Identity=33.6065573770492, Blast_Score=130, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17560910, Length=248, Percent_Identity=31.4516129032258, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17540714, Length=198, Percent_Identity=33.3333333333333, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17540306, Length=265, Percent_Identity=28.6792452830189, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17534483, Length=203, Percent_Identity=29.5566502463054, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17536985, Length=266, Percent_Identity=25.187969924812, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17535521, Length=258, Percent_Identity=27.906976744186, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17549921, Length=215, Percent_Identity=27.906976744186, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI20129971, Length=244, Percent_Identity=32.7868852459016, Blast_Score=118, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24653477, Length=244, Percent_Identity=32.7868852459016, Blast_Score=118, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24653139, Length=251, Percent_Identity=32.6693227091633, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21357171, Length=265, Percent_Identity=30.188679245283, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24650670, Length=254, Percent_Identity=29.1338582677165, Blast_Score=104, Evalue=5e-23,
Organism=Drosophila melanogaster, GI19920382, Length=211, Percent_Identity=29.8578199052133, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19922422, Length=274, Percent_Identity=28.4671532846715, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24654903, Length=197, Percent_Identity=27.4111675126904, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 29056; Mature: 28924

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDAQVTEKQPDALVEQRGHTLVVTLNRPESRNALSTEMLSIMVDAWDRVDNDPEIRSCI
CCCCCCCCCCCHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHEEE
LTGAGGYFCAGMDLKAATAKPPGDSFKDGSYDPSRIDGLLKGRRLTKPLIAAVEGPAIAG
EECCCCEEEECCCEEECCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCC
GTEILQGTDIRVAGESAKFGISEAKWSLYPMGGSAVRLVRQIPYTIACDLLLTGRHITAA
CCHHCCCCCEEEECCCCCCCCCCCCEEEEECCHHHHHHHHHCCHHEEEEEEEECCEEEHH
EAKEYGLIGYVVPDGTALDKALEIAEVINNNGPLAVQAILRSIRETEGLHENEAFKIDTK
HHHHCCEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECC
IGIEVFLSEDAKEGPRAFKEKRAPNFKMK
CCEEEEECCCCCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SDAQVTEKQPDALVEQRGHTLVVTLNRPESRNALSTEMLSIMVDAWDRVDNDPEIRSCI
CCCCCCCCCCHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHEEE
LTGAGGYFCAGMDLKAATAKPPGDSFKDGSYDPSRIDGLLKGRRLTKPLIAAVEGPAIAG
EECCCCEEEECCCEEECCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCCCCC
GTEILQGTDIRVAGESAKFGISEAKWSLYPMGGSAVRLVRQIPYTIACDLLLTGRHITAA
CCHHCCCCCEEEECCCCCCCCCCCCEEEEECCHHHHHHHHHCCHHEEEEEEEECCEEEHH
EAKEYGLIGYVVPDGTALDKALEIAEVINNNGPLAVQAILRSIRETEGLHENEAFKIDTK
HHHHCCEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCEEEECC
IGIEVFLSEDAKEGPRAFKEKRAPNFKMK
CCEEEEECCCCCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]