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The map label for this gene is dus [H]

Identifier: 119870628

GI number: 119870628

Start: 4828244

End: 4829356

Strand: Direct

Name: dus [H]

Synonym: Mkms_4599

Alternate gene names: 119870628

Gene position: 4828244-4829356 (Clockwise)

Preceding gene: 119870627

Following gene: 119870629

Centisome position: 84.16

GC content: 70.71

Gene sequence:

>1113_bases
TTGCCGAGCCCCGTGGTGCTGGCCCCGATGGCCGGGGTGACCAACGTCGCCTTCCGCACCCTGTGCCGTGAGCTCGAGGT
GGCCAGGGCCGGCACCGTCAGCGGCCTGTACGTGTGCGAGATGGTGACCGCCCGGGCGCTGGTCGAACGCCACCCGGTCA
CCATGCACATGACCGCGTTCGCATCCGACGAGTCACCCCGATCGCTGCAGCTCTACACCGTCGACCCGGCCACCACCTAT
GCGGCGGCGAAGATGATCGCCGACGAGAACATGGCCGACCACATCGACATGAACTTCGGCTGCCCGGTGCCCAAGGTGAC
CAAGCGCGGCGGCGGCGCCGCGTTGCCCTACAAGCGGAAACTGTTCGGGCAGATTGTGCGCGCCGCAGTCACCGCCACAG
AGGGCACGGACATCCCCGTCACGGTGAAGTTCCGGATCGGTATCGACGACGCCCACCACACCCATTTGGACGCCGGCCGC
ATCGCCGCCGAGGAGGGTGCGGCCGCGGTCGCCCTGCACGCCCGCACGGCGGCGCAGCGCTACTCCGGCACCGCCGACTG
GGACCAGATCGCCGCGCTCAAGGCGCACGTGACGGCCGTCCCGGTCCTGGGCAACGGCGACATCTTCGACGCCGACGACG
CGCTGGCGATGATGGCGGCCACCGGGTGCGACGGTGTCGTGATCGGGCGCGGCTGCCTCGGACGGCCGTGGCTGTTCGCC
GAACTGTCGGCCAAGTTCACCGGCCGCCCCCTGCCGGTACCGCCCACACTCGGCGAGGTGGCCGACATCATCCGCAGGCA
CGGCGAACTGCTCACCGACCACTTCGGCGAGGACAAGGCCATGCGCGACATCCGCAAACACATCGCGTGGTACCTGCACG
GATTCCCCGCCGGATCGGATCTGCGCCGGGCGCTCGCGCTGGTCAAGACGCGCGCAGAACTCTCCGAACTGCTCGCCCGG
CTCGACCCCGACGTGCCGTTCCCACCGGCGGCGACCGGCCCGCGCGGACGGCAGGGGTCCGCCGCTTCGGTGTCCCTGCC
GGAGGGCTGGCTGACCGATCCGGACGACTGCACGGTGCCGGCGGGTGCAGACGTCATGCACTCCGGAGGCTGA

Upstream 100 bases:

>100_bases
TGGCCGAGAAGAAGGCCGGAAAGGTCCTCGCGTCATCAGCGTCGTAGCACCTCCCGCCGATACGGCCCGGTCTGCAGCCC
TGCGGATCGGGCCGCTGGCA

Downstream 100 bases:

>100_bases
CCGGTCGTCATACCGAGACGTTCTCGAGACGTTCTCCGGCTGGATGCCTCACAGGATCGCTCAGTACGATGTGGGTTCAA
TTGCAGCCCGGCTCCGGTGG

Product: nifR3 family TIM-barrel protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MPSPVVLAPMAGVTNVAFRTLCRELEVARAGTVSGLYVCEMVTARALVERHPVTMHMTAFASDESPRSLQLYTVDPATTY
AAAKMIADENMADHIDMNFGCPVPKVTKRGGGAALPYKRKLFGQIVRAAVTATEGTDIPVTVKFRIGIDDAHHTHLDAGR
IAAEEGAAAVALHARTAAQRYSGTADWDQIAALKAHVTAVPVLGNGDIFDADDALAMMAATGCDGVVIGRGCLGRPWLFA
ELSAKFTGRPLPVPPTLGEVADIIRRHGELLTDHFGEDKAMRDIRKHIAWYLHGFPAGSDLRRALALVKTRAELSELLAR
LDPDVPFPPAATGPRGRQGSAASVSLPEGWLTDPDDCTVPAGADVMHSGG

Sequences:

>Translated_370_residues
MPSPVVLAPMAGVTNVAFRTLCRELEVARAGTVSGLYVCEMVTARALVERHPVTMHMTAFASDESPRSLQLYTVDPATTY
AAAKMIADENMADHIDMNFGCPVPKVTKRGGGAALPYKRKLFGQIVRAAVTATEGTDIPVTVKFRIGIDDAHHTHLDAGR
IAAEEGAAAVALHARTAAQRYSGTADWDQIAALKAHVTAVPVLGNGDIFDADDALAMMAATGCDGVVIGRGCLGRPWLFA
ELSAKFTGRPLPVPPTLGEVADIIRRHGELLTDHFGEDKAMRDIRKHIAWYLHGFPAGSDLRRALALVKTRAELSELLAR
LDPDVPFPPAATGPRGRQGSAASVSLPEGWLTDPDDCTVPAGADVMHSGG
>Mature_369_residues
PSPVVLAPMAGVTNVAFRTLCRELEVARAGTVSGLYVCEMVTARALVERHPVTMHMTAFASDESPRSLQLYTVDPATTYA
AAKMIADENMADHIDMNFGCPVPKVTKRGGGAALPYKRKLFGQIVRAAVTATEGTDIPVTVKFRIGIDDAHHTHLDAGRI
AAEEGAAAVALHARTAAQRYSGTADWDQIAALKAHVTAVPVLGNGDIFDADDALAMMAATGCDGVVIGRGCLGRPWLFAE
LSAKFTGRPLPVPPTLGEVADIIRRHGELLTDHFGEDKAMRDIRKHIAWYLHGFPAGSDLRRALALVKTRAELSELLARL
DPDVPFPPAATGPRGRQGSAASVSLPEGWLTDPDDCTVPAGADVMHSGG

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=306, Percent_Identity=30.3921568627451, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI239788462, Length=296, Percent_Identity=30.7432432432432, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI31742496, Length=237, Percent_Identity=29.957805907173, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI40807366, Length=361, Percent_Identity=27.1468144044321, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1789660, Length=315, Percent_Identity=34.6031746031746, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1788462, Length=280, Percent_Identity=28.5714285714286, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI145693211, Length=311, Percent_Identity=26.3665594855305, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17543114, Length=286, Percent_Identity=28.6713286713287, Blast_Score=110, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI25144369, Length=231, Percent_Identity=30.3030303030303, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17507177, Length=243, Percent_Identity=28.8065843621399, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323433, Length=225, Percent_Identity=25.3333333333333, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323437, Length=240, Percent_Identity=29.1666666666667, Blast_Score=90, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6323560, Length=315, Percent_Identity=25.7142857142857, Blast_Score=86, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24585320, Length=276, Percent_Identity=30.4347826086957, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24580595, Length=241, Percent_Identity=29.045643153527, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19920448, Length=241, Percent_Identity=29.045643153527, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19921524, Length=236, Percent_Identity=27.9661016949153, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 39267; Mature: 39136

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSPVVLAPMAGVTNVAFRTLCRELEVARAGTVSGLYVCEMVTARALVERHPVTMHMTAF
CCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEE
ASDESPRSLQLYTVDPATTYAAAKMIADENMADHIDMNFGCPVPKVTKRGGGAALPYKRK
ECCCCCCEEEEEEECCHHHHHHHHHHHCCCCHHHCCCCCCCCCCHHHCCCCCCCCCHHHH
LFGQIVRAAVTATEGTDIPVTVKFRIGIDDAHHTHLDAGRIAAEEGAAAVALHARTAAQR
HHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCEECCCCCEEEEEHHHHHHHH
YSGTADWDQIAALKAHVTAVPVLGNGDIFDADDALAMMAATGCDGVVIGRGCLGRPWLFA
CCCCCCHHHHHHHHHHEEEEEEECCCCEECCCHHHHHHHHCCCCCEEECCCCCCCCEEEE
ELSAKFTGRPLPVPPTLGEVADIIRRHGELLTDHFGEDKAMRDIRKHIAWYLHGFPAGSD
EHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHH
LRRALALVKTRAELSELLARLDPDVPFPPAATGPRGRQGSAASVSLPEGWLTDPDDCTVP
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCC
AGADVMHSGG
CCCCCCCCCC
>Mature Secondary Structure 
PSPVVLAPMAGVTNVAFRTLCRELEVARAGTVSGLYVCEMVTARALVERHPVTMHMTAF
CCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEE
ASDESPRSLQLYTVDPATTYAAAKMIADENMADHIDMNFGCPVPKVTKRGGGAALPYKRK
ECCCCCCEEEEEEECCHHHHHHHHHHHCCCCHHHCCCCCCCCCCHHHCCCCCCCCCHHHH
LFGQIVRAAVTATEGTDIPVTVKFRIGIDDAHHTHLDAGRIAAEEGAAAVALHARTAAQR
HHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCCCEECCCCCEEEEEHHHHHHHH
YSGTADWDQIAALKAHVTAVPVLGNGDIFDADDALAMMAATGCDGVVIGRGCLGRPWLFA
CCCCCCHHHHHHHHHHEEEEEEECCCCEECCCHHHHHHHHCCCCCEEECCCCCCCCEEEE
ELSAKFTGRPLPVPPTLGEVADIIRRHGELLTDHFGEDKAMRDIRKHIAWYLHGFPAGSD
EHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHH
LRRALALVKTRAELSELLARLDPDVPFPPAATGPRGRQGSAASVSLPEGWLTDPDDCTVP
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCC
AGADVMHSGG
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]