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The map label for this gene is ycdT [H]

Identifier: 119870558

GI number: 119870558

Start: 4762122

End: 4763207

Strand: Direct

Name: ycdT [H]

Synonym: Mkms_4529

Alternate gene names: 119870558

Gene position: 4762122-4763207 (Clockwise)

Preceding gene: 119870557

Following gene: 119870563

Centisome position: 83.0

GC content: 69.61

Gene sequence:

>1086_bases
ATGGCGGCGGCGTTGTCGCGTGGGTGGTGGTCGCACGCCCACCATTACGAGTGGATGAGCGACTACCTGGTCGCCCGCGG
GATGACGGCGGCGGTGCGGGTGATGATGGCAGGCATCGCGGCTTCGCTGTCGGTCTGCCTGCTCGTCCTGCTGCGATTCG
ACGGGCCGCAGGACACCGTCCCGGTCGCGATGACATGGACCGCGGTCGCCGGGGGGGTCTTCGGGGTGGCGTTGTGGGCG
CGGCGCTGGCCGACCCACACCGAGTCGGTGGCCTTCGCCGTCGTGTCCAACGCCGCGATCGCGCTGTCGTGTCTGGCCTA
TCCCAACCCCACCGGTGCGCTCACCGGGTGCATCTCCTTCGCGACCATCGCCGCCTACATCGCGTTCTTCCACTCCACGG
CGCTGGTGGTCTACAACTTCGCGGTGACGACGGCCGTCGCGGTCATCGCCGCGCTGCGGATCGCGCTGTCCGGGCATCCC
GCGATGGCCGTCGTCGACCTGTTCATCGTGGTGCAGATCAACATCGCGATGCCGGTCGCGATCCACGTCCTGGTCCGGGC
GCTCGGCGTGGATCTGGCGCGCGCCGATCGGGATCCGCTCACCGGGCTCCTCAACCGTCGCGCCTTCTATGCGCACAGCG
CGAAACTGCTCACCGGCGGGCCGTCGCACGGTGGGACCCACCTGGTCATGGTGGTGGTGGACCTCGACGACTTCAAGCGG
ATCAACGACACCTACGGCCATCAGGTCGGCGATGAGGCGCTGATCTCGGTCGGGCAGGCGCTGTCCCGCGCCGTCGTCGG
GTTCCCGGCGGAGATCGGCCGCAGCGGTGGCGAGGAGTTCGTGGTCGCCGCCGTATTGGAGACCGACGATCCCGCTCCGC
TGGCCGAGCAGATCTGTGGGGAGATCGCCGCGCTGCCGGAGCCCGTCACGGCGAGTGTGGGAACGGCGTGCATCGCGCTC
GACGCGTCGCGGGGCAACCACGAGTCGACGCTGGACCGGATGGTCCGGGCCGCCGACGCAGCGATGTACGAGGCGAAACG
GGCCGGTGGGAACAAGTGCCACCACCTCGGCGAGCCGTACTGGTGA

Upstream 100 bases:

>100_bases
GCTCACCGAAGTGTGAACTTCGCGTCCGGTGGCCGTCGGTATCTGGTTAAGTTTGCCAAGGGAAGTACTTTCGATCAGTT
GTGAGCCGGGGGCGGGGAAG

Downstream 100 bases:

>100_bases
GGTCGGGGTTGATTCTCGCGCGAGACTGAACTCACGCAGCCAAAGTACGAGAAGCCCCCTGCGACACTTCAGCCTCGCGA
ACCCTTCGCGCATGCCCAAC

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 361; Mature: 360

Protein sequence:

>361_residues
MAAALSRGWWSHAHHYEWMSDYLVARGMTAAVRVMMAGIAASLSVCLLVLLRFDGPQDTVPVAMTWTAVAGGVFGVALWA
RRWPTHTESVAFAVVSNAAIALSCLAYPNPTGALTGCISFATIAAYIAFFHSTALVVYNFAVTTAVAVIAALRIALSGHP
AMAVVDLFIVVQINIAMPVAIHVLVRALGVDLARADRDPLTGLLNRRAFYAHSAKLLTGGPSHGGTHLVMVVVDLDDFKR
INDTYGHQVGDEALISVGQALSRAVVGFPAEIGRSGGEEFVVAAVLETDDPAPLAEQICGEIAALPEPVTASVGTACIAL
DASRGNHESTLDRMVRAADAAMYEAKRAGGNKCHHLGEPYW

Sequences:

>Translated_361_residues
MAAALSRGWWSHAHHYEWMSDYLVARGMTAAVRVMMAGIAASLSVCLLVLLRFDGPQDTVPVAMTWTAVAGGVFGVALWA
RRWPTHTESVAFAVVSNAAIALSCLAYPNPTGALTGCISFATIAAYIAFFHSTALVVYNFAVTTAVAVIAALRIALSGHP
AMAVVDLFIVVQINIAMPVAIHVLVRALGVDLARADRDPLTGLLNRRAFYAHSAKLLTGGPSHGGTHLVMVVVDLDDFKR
INDTYGHQVGDEALISVGQALSRAVVGFPAEIGRSGGEEFVVAAVLETDDPAPLAEQICGEIAALPEPVTASVGTACIAL
DASRGNHESTLDRMVRAADAAMYEAKRAGGNKCHHLGEPYW
>Mature_360_residues
AAALSRGWWSHAHHYEWMSDYLVARGMTAAVRVMMAGIAASLSVCLLVLLRFDGPQDTVPVAMTWTAVAGGVFGVALWAR
RWPTHTESVAFAVVSNAAIALSCLAYPNPTGALTGCISFATIAAYIAFFHSTALVVYNFAVTTAVAVIAALRIALSGHPA
MAVVDLFIVVQINIAMPVAIHVLVRALGVDLARADRDPLTGLLNRRAFYAHSAKLLTGGPSHGGTHLVMVVVDLDDFKRI
NDTYGHQVGDEALISVGQALSRAVVGFPAEIGRSGGEEFVVAAVLETDDPAPLAEQICGEIAALPEPVTASVGTACIALD
ASRGNHESTLDRMVRAADAAMYEAKRAGGNKCHHLGEPYW

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=195, Percent_Identity=31.7948717948718, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI145693134, Length=178, Percent_Identity=34.2696629213483, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787816, Length=169, Percent_Identity=33.7278106508876, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1786584, Length=164, Percent_Identity=31.7073170731707, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788085, Length=189, Percent_Identity=29.6296296296296, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI87081977, Length=198, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787802, Length=192, Percent_Identity=30.2083333333333, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081881, Length=166, Percent_Identity=34.9397590361446, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788956, Length=167, Percent_Identity=32.3353293413174, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1787541, Length=166, Percent_Identity=34.3373493975904, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87082007, Length=160, Percent_Identity=31.25, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 38096; Mature: 37965

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAALSRGWWSHAHHYEWMSDYLVARGMTAAVRVMMAGIAASLSVCLLVLLRFDGPQDTV
CCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PVAMTWTAVAGGVFGVALWARRWPTHTESVAFAVVSNAAIALSCLAYPNPTGALTGCISF
CEEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHEEEEECCCCCCHHHHHHHHH
ATIAAYIAFFHSTALVVYNFAVTTAVAVIAALRIALSGHPAMAVVDLFIVVQINIAMPVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEHHHHHHH
IHVLVRALGVDLARADRDPLTGLLNRRAFYAHSAKLLTGGPSHGGTHLVMVVVDLDDFKR
HHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEEECHHHHH
INDTYGHQVGDEALISVGQALSRAVVGFPAEIGRSGGEEFVVAAVLETDDPAPLAEQICG
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEEEEECCCCCHHHHHHHH
EIAALPEPVTASVGTACIALDASRGNHESTLDRMVRAADAAMYEAKRAGGNKCHHLGEPY
HHHCCCCCCHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
W
C
>Mature Secondary Structure 
AAALSRGWWSHAHHYEWMSDYLVARGMTAAVRVMMAGIAASLSVCLLVLLRFDGPQDTV
CCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PVAMTWTAVAGGVFGVALWARRWPTHTESVAFAVVSNAAIALSCLAYPNPTGALTGCISF
CEEEEHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHEEEEECCCCCCHHHHHHHHH
ATIAAYIAFFHSTALVVYNFAVTTAVAVIAALRIALSGHPAMAVVDLFIVVQINIAMPVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHEEHHHHHHH
IHVLVRALGVDLARADRDPLTGLLNRRAFYAHSAKLLTGGPSHGGTHLVMVVVDLDDFKR
HHHHHHHHCCHHHHCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEEECHHHHH
INDTYGHQVGDEALISVGQALSRAVVGFPAEIGRSGGEEFVVAAVLETDDPAPLAEQICG
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHCCCCCCCEEEEEEEECCCCCHHHHHHHH
EIAALPEPVTASVGTACIALDASRGNHESTLDRMVRAADAAMYEAKRAGGNKCHHLGEPY
HHHCCCCCCHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
W
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]