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The map label for this gene is fadK [H]

Identifier: 119870526

GI number: 119870526

Start: 4729770

End: 4731272

Strand: Direct

Name: fadK [H]

Synonym: Mkms_4497

Alternate gene names: 119870526

Gene position: 4729770-4731272 (Clockwise)

Preceding gene: 119870525

Following gene: 119870527

Centisome position: 82.44

GC content: 67.93

Gene sequence:

>1503_bases
GTGGGTACCACGCTGGCCGATGCCCTGCGCGATGCCGCGCGGGACACACCGGACCGCGTGCTGGTGCGCGACGGCGACGA
TGAACTCACCTGTGGCGCACTGCAAGAACGCGCGATGGCATTGGCGTCGGCACTGATGCGGCGGATGCCGGTCGGCAGCG
TGGTGTCATTCATGCTGCCCAACTGGCACGAGGCCGCCGTCGTCTATCTCGGCGCCACGCTGGCGGGTATGGTCGTGAAC
CCGATCCTGCCATCGCTGCGCGACCACGAACTCACGTTCATCCTCGCCGACGCCGACAGTCGGGCGATCTTCATCCCCCA
CACGTTCAACGGCCACGACTACGCCGCCATGCTCAAACGCGTCGCCGCAGGGCTGCCCTCACCCCCAGAGGTCGTCGTGG
TGCGGGCGGAACCGGCCGGACACAGTTCCTTCGCGGATTTGCTCGCCGAAGCCGGCGGCGGTGACCTACCCGAGCTGAGC
TCCGATTCCACCCGCATGATCATGTACACCTCGGGCACCACCGGGCGGCCCAAGGGCGTTCTGCACAGCCATGATTCGCT
GCACGCACTGCTCGCGCAGCTGGGCCGGTACTGGAACATCGACCCCGGCGACACGTTCCTGGTGCCCTCACCGATCGCCC
ACATCGGTGGGTCGATCTACGCCTTCGAGGCACCGCTGCTGCTCGGCTCCAGGGCGGTACTCATGGAACGCTGGGATCCC
GACGGCGCCGTGGCGTTGATGCTCGAGCACGGATGCACGCACATGGCCGGGGCCACACCGTTTCTCGACGGTCTGCTCGC
CGCGGCGCAGCGGGCGGACACCCGACTACCGGACCTCGAGGTGTTCATCTGTGGCGGCGCCTCGGTCCCGCCGTCGCTGA
TCCGCAGGGCGGCGGCCTATTTCGAGCGTGCGCTGGTGACCCGCGTGTACGGTTCGACAGAAGTCCCGGTGACCACGGTC
GGATCCCTCGACGATGTGGACCACGCCGCCGAGACCGACGGCCGCCCCGGCATCGCCGATGTCACGTTGGTGGACGGGGA
GATCCGCGCCCGCGGGCCGCAGATGTTCACCGGTTACCTCCACCCGCAGGACAACACCGGAGCCTTCGACACCGCTGGAT
ACTTCCGCACCGGTGACCTCGGCCGCTGGACCGACGATGGCTACCTGATGGTCACCGGCCGCGCCAAGGACATCATCATC
CGCAACGGCGAGAACATCTCGCCCAAGGAGGTGGAGGACCTTCTGGTCACCCACCCGCAGATCGCCGAGATCGCGATCGT
CGGCATCCCCGATGCGCGCACCGGCGAACGCGCCTGCGCCGCCGTGGTGGCGGCCGGCGATCCGGGACCCGACGTCGCGG
CGTTGCGTGAGTTCCTGCTGGGCCGCGGGGTGGCCAAGTTCAAGGTGCCCGAACAGGTGGTGCTGGTGGAGGCGTTGCCG
AAGAACGATGCGGGCAAGGTGCTCAAGCACAAGATCAGGGCATCGCTGACGGAAGGTGGATGA

Upstream 100 bases:

>100_bases
ACACCACCTCCGACGGTGAGGTCGCACAGGTCACGGTGAACACCCATCCGTCGGCGCGCTACCGGCACTCGATGACGATG
CGCCGGGTCAGGGACTGACG

Downstream 100 bases:

>100_bases
CGTGCAGGTAGCGATCGTCACGGGTGCCAGCAGCGGCATCGGGTTCGGTTGTGCCACAACGCTGGCCGAGCAGGGTATGG
CGGTGCTGGGCACCGGACGA

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MGTTLADALRDAARDTPDRVLVRDGDDELTCGALQERAMALASALMRRMPVGSVVSFMLPNWHEAAVVYLGATLAGMVVN
PILPSLRDHELTFILADADSRAIFIPHTFNGHDYAAMLKRVAAGLPSPPEVVVVRAEPAGHSSFADLLAEAGGGDLPELS
SDSTRMIMYTSGTTGRPKGVLHSHDSLHALLAQLGRYWNIDPGDTFLVPSPIAHIGGSIYAFEAPLLLGSRAVLMERWDP
DGAVALMLEHGCTHMAGATPFLDGLLAAAQRADTRLPDLEVFICGGASVPPSLIRRAAAYFERALVTRVYGSTEVPVTTV
GSLDDVDHAAETDGRPGIADVTLVDGEIRARGPQMFTGYLHPQDNTGAFDTAGYFRTGDLGRWTDDGYLMVTGRAKDIII
RNGENISPKEVEDLLVTHPQIAEIAIVGIPDARTGERACAAVVAAGDPGPDVAALREFLLGRGVAKFKVPEQVVLVEALP
KNDAGKVLKHKIRASLTEGG

Sequences:

>Translated_500_residues
MGTTLADALRDAARDTPDRVLVRDGDDELTCGALQERAMALASALMRRMPVGSVVSFMLPNWHEAAVVYLGATLAGMVVN
PILPSLRDHELTFILADADSRAIFIPHTFNGHDYAAMLKRVAAGLPSPPEVVVVRAEPAGHSSFADLLAEAGGGDLPELS
SDSTRMIMYTSGTTGRPKGVLHSHDSLHALLAQLGRYWNIDPGDTFLVPSPIAHIGGSIYAFEAPLLLGSRAVLMERWDP
DGAVALMLEHGCTHMAGATPFLDGLLAAAQRADTRLPDLEVFICGGASVPPSLIRRAAAYFERALVTRVYGSTEVPVTTV
GSLDDVDHAAETDGRPGIADVTLVDGEIRARGPQMFTGYLHPQDNTGAFDTAGYFRTGDLGRWTDDGYLMVTGRAKDIII
RNGENISPKEVEDLLVTHPQIAEIAIVGIPDARTGERACAAVVAAGDPGPDVAALREFLLGRGVAKFKVPEQVVLVEALP
KNDAGKVLKHKIRASLTEGG
>Mature_499_residues
GTTLADALRDAARDTPDRVLVRDGDDELTCGALQERAMALASALMRRMPVGSVVSFMLPNWHEAAVVYLGATLAGMVVNP
ILPSLRDHELTFILADADSRAIFIPHTFNGHDYAAMLKRVAAGLPSPPEVVVVRAEPAGHSSFADLLAEAGGGDLPELSS
DSTRMIMYTSGTTGRPKGVLHSHDSLHALLAQLGRYWNIDPGDTFLVPSPIAHIGGSIYAFEAPLLLGSRAVLMERWDPD
GAVALMLEHGCTHMAGATPFLDGLLAAAQRADTRLPDLEVFICGGASVPPSLIRRAAAYFERALVTRVYGSTEVPVTTVG
SLDDVDHAAETDGRPGIADVTLVDGEIRARGPQMFTGYLHPQDNTGAFDTAGYFRTGDLGRWTDDGYLMVTGRAKDIIIR
NGENISPKEVEDLLVTHPQIAEIAIVGIPDARTGERACAAVVAAGDPGPDVAALREFLLGRGVAKFKVPEQVVLVEALPK
NDAGKVLKHKIRASLTEGG

Specific function: Catalyzes the esterification, concomitant with transport, of exogenous fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids. It has a low activity on medium or long chain fatty acids and is ma

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Membrane; Peripheral membrane protein. Note=Partially membrane-associated [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=548, Percent_Identity=27.007299270073, Blast_Score=172, Evalue=8e-43,
Organism=Homo sapiens, GI187761345, Length=539, Percent_Identity=26.3450834879406, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI187761343, Length=539, Percent_Identity=26.3450834879406, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI157311624, Length=469, Percent_Identity=26.865671641791, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI157311622, Length=469, Percent_Identity=26.865671641791, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI58082049, Length=469, Percent_Identity=26.865671641791, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI42544132, Length=384, Percent_Identity=26.0416666666667, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI38505220, Length=469, Percent_Identity=25.7995735607676, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI115511026, Length=483, Percent_Identity=24.4306418219462, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI122937307, Length=468, Percent_Identity=24.7863247863248, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI28416953, Length=128, Percent_Identity=35.9375, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI145693145, Length=518, Percent_Identity=34.1698841698842, Blast_Score=279, Evalue=3e-76,
Organism=Escherichia coli, GI1788107, Length=536, Percent_Identity=27.0522388059701, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1786810, Length=512, Percent_Identity=27.9296875, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI221142682, Length=466, Percent_Identity=26.1802575107296, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1790505, Length=556, Percent_Identity=23.3812949640288, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1789201, Length=483, Percent_Identity=25.2587991718427, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788595, Length=329, Percent_Identity=25.8358662613982, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786529, Length=398, Percent_Identity=28.391959798995, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1786801, Length=456, Percent_Identity=24.1228070175439, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17558820, Length=498, Percent_Identity=29.718875502008, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI32563687, Length=492, Percent_Identity=27.4390243902439, Blast_Score=163, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI71994690, Length=548, Percent_Identity=26.6423357664234, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI71994703, Length=548, Percent_Identity=26.6423357664234, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI71994694, Length=548, Percent_Identity=26.6423357664234, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17538037, Length=498, Percent_Identity=25.5020080321285, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17557194, Length=501, Percent_Identity=26.1477045908184, Blast_Score=142, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17560308, Length=548, Percent_Identity=25.1824817518248, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17559526, Length=535, Percent_Identity=25.607476635514, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17531443, Length=469, Percent_Identity=22.6012793176972, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71985884, Length=361, Percent_Identity=28.808864265928, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17560140, Length=515, Percent_Identity=23.8834951456311, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71996755, Length=462, Percent_Identity=23.5930735930736, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25141359, Length=359, Percent_Identity=22.2841225626741, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319699, Length=483, Percent_Identity=25.0517598343685, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6319264, Length=561, Percent_Identity=24.4206773618538, Blast_Score=100, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6323182, Length=550, Percent_Identity=24, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24581924, Length=513, Percent_Identity=28.0701754385965, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI18859661, Length=505, Percent_Identity=25.9405940594059, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24656500, Length=506, Percent_Identity=28.4584980237154, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI19922652, Length=494, Percent_Identity=25.5060728744939, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24653035, Length=449, Percent_Identity=25.1670378619154, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24648257, Length=390, Percent_Identity=24.6153846153846, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21355181, Length=353, Percent_Identity=26.0623229461756, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI281365686, Length=476, Percent_Identity=24.5798319327731, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI20130357, Length=353, Percent_Identity=25.4957507082153, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI21358303, Length=357, Percent_Identity=23.8095238095238, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI24648260, Length=476, Percent_Identity=24.7899159663866, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI161076582, Length=457, Percent_Identity=23.8512035010941, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24667959, Length=370, Percent_Identity=25.1351351351351, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI62472339, Length=370, Percent_Identity=25.1351351351351, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24667955, Length=364, Percent_Identity=25.5494505494505, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI21356441, Length=393, Percent_Identity=21.882951653944, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21356947, Length=389, Percent_Identity=24.6786632390745, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24648255, Length=351, Percent_Identity=22.5071225071225, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648253, Length=347, Percent_Identity=21.9020172910663, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: NA

Molecular weight: Translated: 53138; Mature: 53007

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTTLADALRDAARDTPDRVLVRDGDDELTCGALQERAMALASALMRRMPVGSVVSFMLP
CCCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC
NWHEAAVVYLGATLAGMVVNPILPSLRDHELTFILADADSRAIFIPHTFNGHDYAAMLKR
CCCHHEEEEHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEECCCCCCHHHHHHHH
VAAGLPSPPEVVVVRAEPAGHSSFADLLAEAGGGDLPELSSDSTRMIMYTSGTTGRPKGV
HHCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCC
LHSHDSLHALLAQLGRYWNIDPGDTFLVPSPIAHIGGSIYAFEAPLLLGSRAVLMERWDP
EECCHHHHHHHHHHCCCCCCCCCCEEECCCCHHHHCCCEEEEECCHHHCCCEEEEEEECC
DGAVALMLEHGCTHMAGATPFLDGLLAAAQRADTRLPDLEVFICGGASVPPSLIRRAAAY
CCEEEEEEECCCCHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHH
FERALVTRVYGSTEVPVTTVGSLDDVDHAAETDGRPGIADVTLVDGEIRARGPQMFTGYL
HHHHHHHHHCCCCCCCEEECCCCCCCHHHHCCCCCCCCEEEEEECCCCCCCCCCEEEEEE
HPQDNTGAFDTAGYFRTGDLGRWTDDGYLMVTGRAKDIIIRNGENISPKEVEDLLVTHPQ
CCCCCCCCCCCCCCEECCCCCCCCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHCCCC
IAEIAIVGIPDARTGERACAAVVAAGDPGPDVAALREFLLGRGVAKFKVPEQVVLVEALP
EEEEEEEECCCCCCCCHHEEEEEECCCCCCCHHHHHHHHHHCCCEEECCCCEEEEEEECC
KNDAGKVLKHKIRASLTEGG
CCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GTTLADALRDAARDTPDRVLVRDGDDELTCGALQERAMALASALMRRMPVGSVVSFMLP
CCHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCC
NWHEAAVVYLGATLAGMVVNPILPSLRDHELTFILADADSRAIFIPHTFNGHDYAAMLKR
CCCHHEEEEHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCEEEEECCCCCCHHHHHHHH
VAAGLPSPPEVVVVRAEPAGHSSFADLLAEAGGGDLPELSSDSTRMIMYTSGTTGRPKGV
HHCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCC
LHSHDSLHALLAQLGRYWNIDPGDTFLVPSPIAHIGGSIYAFEAPLLLGSRAVLMERWDP
EECCHHHHHHHHHHCCCCCCCCCCEEECCCCHHHHCCCEEEEECCHHHCCCEEEEEEECC
DGAVALMLEHGCTHMAGATPFLDGLLAAAQRADTRLPDLEVFICGGASVPPSLIRRAAAY
CCEEEEEEECCCCHHCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHH
FERALVTRVYGSTEVPVTTVGSLDDVDHAAETDGRPGIADVTLVDGEIRARGPQMFTGYL
HHHHHHHHHCCCCCCCEEECCCCCCCHHHHCCCCCCCCEEEEEECCCCCCCCCCEEEEEE
HPQDNTGAFDTAGYFRTGDLGRWTDDGYLMVTGRAKDIIIRNGENISPKEVEDLLVTHPQ
CCCCCCCCCCCCCCEECCCCCCCCCCCEEEEECCCCEEEEECCCCCCHHHHHHHHHCCCC
IAEIAIVGIPDARTGERACAAVVAAGDPGPDVAALREFLLGRGVAKFKVPEQVVLVEALP
EEEEEEEECCCCCCCCHHEEEEEECCCCCCCHHHHHHHHHHCCCEEECCCCEEEEEEECC
KNDAGKVLKHKIRASLTEGG
CCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 7984428; 12535077 [H]