Click here to switch to the map view.

The map label for this gene is ada [H]

Identifier: 119869974

GI number: 119869974

Start: 4152463

End: 4154016

Strand: Direct

Name: ada [H]

Synonym: Mkms_3944

Alternate gene names: 119869974

Gene position: 4152463-4154016 (Clockwise)

Preceding gene: 119869972

Following gene: 119869975

Centisome position: 72.38

GC content: 71.75

Gene sequence:

>1554_bases
ATGGCGCACGACCATGTCGGATTTCCGCCGGTGGTGTCGGTGGCGGCGTGTAAGTCTGACAGCATGTACGACGATTTCGA
CCGCTGCTACCGGGCCGTGCAGTCCAAGGACGCGCGGTTCGACGGTTGGTTCGTCACGGCGGTGCTGACGACGCGGATCT
ACTGCCGCCCAAGCTGTCCCGTCCGGCCGCCGTTCGCCCGCAACGTGCGCTTCTATCCGACCGCCGCGGCCGCTCAGGCG
GCGGGATTCCGCGCCTGTAAGCGGTGCAGGCCCGACGCGTCGCCCGGTTCTCCCGAGTGGAACGTCCGCGGCGATGTGGC
CGCCAGGGCGATGCGCCTGATCGCCGACGGCACGGTCGACCGGGACGGTGTCACGGGTCTGGCCGGCCGGCTCGGCTACA
CCACGCGGCAGTTGCAGCGCATCCTGCAGGCCGAGGTGGGGGCGAATCCGCTGGCGTTGGCCCGTGCGCAGCGGGCACAG
ACCGCACGCGTGCTGATCGAGACCACCGACCTGCCGTTCTCCGATGTGGCGTTCGCCGCGGGGTTCTCGAGCATCCGGCA
GTTCAACGACACGGTGCGCGCCACCTCCGCGTGCACCCCGACCGCGATGCGGGAGCGGGCGCGACGCCGCTTCGGGGCGG
CCACCGCCGGCGCGGGGTCACTGTCACTGCGCCTGCCGGTGCGTAGGCCGTTCGCCTACGAAGGGGTGTTCGGGCACCTG
GCGGCCAGCGCCGTACCGGGTGTCGAGGAGTTCCGCGACGGGGCCTTCCGCCGCACGCTGCGGCTTTCGAGGGGCCACGG
CATCGTCGGCCTCACCCCCCGCGACGGTCACGTCGACTGCGTGCTGCACCTCGAGGACCTTCGGGACCTGTCCAGCGCCA
TCGCGCGGTGCCGGCGCCTGCTGGACCTCGACGCCGACCCGGAGGCCGTCGTCGACGTACTCGGCGCCGACCCGGACCTC
ACCGCGTTGGTGACGAAGGCGCCCGGGCAGCGCATCCCGCGCACTGTCGACGAGGCGGAACTGGCCGTGCGGGTGGTTCT
GGGCCAACAGGTGTCCCTGAAGGCCGCCCGCACGCACGCCGCGCGGCTCGTCACCCACTACGGTCGCCCGATCAGCGATC
CACACGGTGGCCTGACCCACGTGTTTCCCACCGTTGAGGAACTCGCCGACATCGCTGCGCCCCATCTGGCGGTGCCGCGC
AGCCGGCAGGCCACCGTCCGCTCGCTCATCGCGGCGCTGGCGTCGGGCGACGTGCGGCTCGATCCCGGATGTGACTGGAA
CGAGGCACGGGCACAACTCACCGTACTGCCCGGCATCGGCACATGGACTGCGGAGGTGATCGCGATGCGCGGACTCGGCG
ATCCCGACGCCTTCCCCGTCACCGATCTGGGCGTGCTCACCGCCGCTCGCCACCTCGGCCTGGCCGAGGATGCCCGGGCC
CTTGCAGCGCACGGCGCCCGGTGGCGTCCGTGGCGGGCCTACGCGACGCAGCACCTGTGGACGGCGCTCGATCATCCGGT
CAACGACTGGCCCCCGAAGGAGATCCGACAGTGA

Upstream 100 bases:

>100_bases
GCGTCGAGGCAGTCCTCGATCTTCAGCAGACCTTCGTGAACCATCTGCCCGGTACCGCCGCTCATGGTTTCGAGTCTAGG
GATGCGGCGGCACGGGCAGC

Downstream 100 bases:

>100_bases
CGATTCGATTCCGTACCGTCGACAGCCCGGTCGGTCTGTTGACCCTCGCCGGCCGCGACGACCGGCTCAGGCATCTGCGC
ATGGTGGATCAGACCTACGA

Product: DNA-O6-methylguanine--protein-cysteine S-methyltransferase / DNA-3-methyladenine glycosylase II / transcriptional regulator Ada

Products: NA

Alternate protein names: Regulatory protein of adaptative response; Methylated-DNA--protein-cysteine methyltransferase; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 517; Mature: 516

Protein sequence:

>517_residues
MAHDHVGFPPVVSVAACKSDSMYDDFDRCYRAVQSKDARFDGWFVTAVLTTRIYCRPSCPVRPPFARNVRFYPTAAAAQA
AGFRACKRCRPDASPGSPEWNVRGDVAARAMRLIADGTVDRDGVTGLAGRLGYTTRQLQRILQAEVGANPLALARAQRAQ
TARVLIETTDLPFSDVAFAAGFSSIRQFNDTVRATSACTPTAMRERARRRFGAATAGAGSLSLRLPVRRPFAYEGVFGHL
AASAVPGVEEFRDGAFRRTLRLSRGHGIVGLTPRDGHVDCVLHLEDLRDLSSAIARCRRLLDLDADPEAVVDVLGADPDL
TALVTKAPGQRIPRTVDEAELAVRVVLGQQVSLKAARTHAARLVTHYGRPISDPHGGLTHVFPTVEELADIAAPHLAVPR
SRQATVRSLIAALASGDVRLDPGCDWNEARAQLTVLPGIGTWTAEVIAMRGLGDPDAFPVTDLGVLTAARHLGLAEDARA
LAAHGARWRPWRAYATQHLWTALDHPVNDWPPKEIRQ

Sequences:

>Translated_517_residues
MAHDHVGFPPVVSVAACKSDSMYDDFDRCYRAVQSKDARFDGWFVTAVLTTRIYCRPSCPVRPPFARNVRFYPTAAAAQA
AGFRACKRCRPDASPGSPEWNVRGDVAARAMRLIADGTVDRDGVTGLAGRLGYTTRQLQRILQAEVGANPLALARAQRAQ
TARVLIETTDLPFSDVAFAAGFSSIRQFNDTVRATSACTPTAMRERARRRFGAATAGAGSLSLRLPVRRPFAYEGVFGHL
AASAVPGVEEFRDGAFRRTLRLSRGHGIVGLTPRDGHVDCVLHLEDLRDLSSAIARCRRLLDLDADPEAVVDVLGADPDL
TALVTKAPGQRIPRTVDEAELAVRVVLGQQVSLKAARTHAARLVTHYGRPISDPHGGLTHVFPTVEELADIAAPHLAVPR
SRQATVRSLIAALASGDVRLDPGCDWNEARAQLTVLPGIGTWTAEVIAMRGLGDPDAFPVTDLGVLTAARHLGLAEDARA
LAAHGARWRPWRAYATQHLWTALDHPVNDWPPKEIRQ
>Mature_516_residues
AHDHVGFPPVVSVAACKSDSMYDDFDRCYRAVQSKDARFDGWFVTAVLTTRIYCRPSCPVRPPFARNVRFYPTAAAAQAA
GFRACKRCRPDASPGSPEWNVRGDVAARAMRLIADGTVDRDGVTGLAGRLGYTTRQLQRILQAEVGANPLALARAQRAQT
ARVLIETTDLPFSDVAFAAGFSSIRQFNDTVRATSACTPTAMRERARRRFGAATAGAGSLSLRLPVRRPFAYEGVFGHLA
ASAVPGVEEFRDGAFRRTLRLSRGHGIVGLTPRDGHVDCVLHLEDLRDLSSAIARCRRLLDLDADPEAVVDVLGADPDLT
ALVTKAPGQRIPRTVDEAELAVRVVLGQQVSLKAARTHAARLVTHYGRPISDPHGGLTHVFPTVEELADIAAPHLAVPRS
RQATVRSLIAALASGDVRLDPGCDWNEARAQLTVLPGIGTWTAEVIAMRGLGDPDAFPVTDLGVLTAARHLGLAEDARAL
AAHGARWRPWRAYATQHLWTALDHPVNDWPPKEIRQ

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788383, Length=292, Percent_Identity=31.1643835616438, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788542, Length=173, Percent_Identity=33.5260115606936, Blast_Score=83, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR010316
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR023170
- InterPro:   IPR012294 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF06029 AlkA_N; PF00730 HhH-GPD [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 56039; Mature: 55908

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHDHVGFPPVVSVAACKSDSMYDDFDRCYRAVQSKDARFDGWFVTAVLTTRIYCRPSCP
CCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCEEHEEHHHHHHEECCCCC
VRPPFARNVRFYPTAAAAQAAGFRACKRCRPDASPGSPEWNVRGDVAARAMRLIADGTVD
CCCCHHCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
RDGVTGLAGRLGYTTRQLQRILQAEVGANPLALARAQRAQTARVLIETTDLPFSDVAFAA
CCCCHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEEEECCCCHHHHHHHH
GFSSIRQFNDTVRATSACTPTAMRERARRRFGAATAGAGSLSLRLPVRRPFAYEGVFGHL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHH
AASAVPGVEEFRDGAFRRTLRLSRGHGIVGLTPRDGHVDCVLHLEDLRDLSSAIARCRRL
HHHHCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHH
LDLDADPEAVVDVLGADPDLTALVTKAPGQRIPRTVDEAELAVRVVLGQQVSLKAARTHA
HCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
ARLVTHYGRPISDPHGGLTHVFPTVEELADIAAPHLAVPRSRQATVRSLIAALASGDVRL
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEE
DPGCDWNEARAQLTVLPGIGTWTAEVIAMRGLGDPDAFPVTDLGVLTAARHLGLAEDARA
CCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHH
LAAHGARWRPWRAYATQHLWTALDHPVNDWPPKEIRQ
HHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHCC
>Mature Secondary Structure 
AHDHVGFPPVVSVAACKSDSMYDDFDRCYRAVQSKDARFDGWFVTAVLTTRIYCRPSCP
CCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCEEHEEHHHHHHEECCCCC
VRPPFARNVRFYPTAAAAQAAGFRACKRCRPDASPGSPEWNVRGDVAARAMRLIADGTVD
CCCCHHCCEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
RDGVTGLAGRLGYTTRQLQRILQAEVGANPLALARAQRAQTARVLIETTDLPFSDVAFAA
CCCCHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHEEEEEEECCCCHHHHHHHH
GFSSIRQFNDTVRATSACTPTAMRERARRRFGAATAGAGSLSLRLPVRRPFAYEGVFGHL
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHH
AASAVPGVEEFRDGAFRRTLRLSRGHGIVGLTPRDGHVDCVLHLEDLRDLSSAIARCRRL
HHHHCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHH
LDLDADPEAVVDVLGADPDLTALVTKAPGQRIPRTVDEAELAVRVVLGQQVSLKAARTHA
HCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
ARLVTHYGRPISDPHGGLTHVFPTVEELADIAAPHLAVPRSRQATVRSLIAALASGDVRL
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCEEE
DPGCDWNEARAQLTVLPGIGTWTAEVIAMRGLGDPDAFPVTDLGVLTAARHLGLAEDARA
CCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHH
LAAHGARWRPWRAYATQHLWTALDHPVNDWPPKEIRQ
HHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]