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The map label for this gene is ogt [H]

Identifier: 119869975

GI number: 119869975

Start: 4154013

End: 4154513

Strand: Direct

Name: ogt [H]

Synonym: Mkms_3945

Alternate gene names: 119869975

Gene position: 4154013-4154513 (Clockwise)

Preceding gene: 119869974

Following gene: 119869977

Centisome position: 72.4

GC content: 63.47

Gene sequence:

>501_bases
GTGACGATTCGATTCCGTACCGTCGACAGCCCGGTCGGTCTGTTGACCCTCGCCGGCCGCGACGACCGGCTCAGGCATCT
GCGCATGGTGGATCAGACCTACGAACCGAGCCGCCAGGGTTGGGAGCCCGATCCGACCGCATTCGGTGACGTCGTGGCGC
AACTCGGCGCCTACTTCGCCGGCGAACTCACGACGTTCGAACTCGAGCTCGACCTGGCCGGAACGGCGTTTCAGCGTCGC
GTCTGGGAAGCGCTACTGACCATTCCGTACGGGGAGACGCGGTCGTACGGCGACATCGCACGACAGATCGGTGCACCGGG
CGCATTTCGGGCAGTCGGACTGGCGAATGGCCACAATCCGATCGGCATCATCGTGCCCTGCCATCGGGTGATCGGCGCGA
ATGGCAGCCTCACCGGATATGGCGGCGGAATCGAACGGAAGCGGATGCTCCTCGGAATGGAAAGGCAATTCCATTCCGCG
GTTCCGACACTATTCGATTGA

Upstream 100 bases:

>100_bases
ACGGCGCCCGGTGGCGTCCGTGGCGGGCCTACGCGACGCAGCACCTGTGGACGGCGCTCGATCATCCGGTCAACGACTGG
CCCCCGAAGGAGATCCGACA

Downstream 100 bases:

>100_bases
TTCCGACTGGGCCGTTGAATAAAAAGGCCGTCAAACAAAAAAGACCTCGGCCCTCCGATTGTGGTCGGAGGGCCGAGGTG
TAAAGGGTGTTCGGCGGTGT

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 166; Mature: 165

Protein sequence:

>166_residues
MTIRFRTVDSPVGLLTLAGRDDRLRHLRMVDQTYEPSRQGWEPDPTAFGDVVAQLGAYFAGELTTFELELDLAGTAFQRR
VWEALLTIPYGETRSYGDIARQIGAPGAFRAVGLANGHNPIGIIVPCHRVIGANGSLTGYGGGIERKRMLLGMERQFHSA
VPTLFD

Sequences:

>Translated_166_residues
MTIRFRTVDSPVGLLTLAGRDDRLRHLRMVDQTYEPSRQGWEPDPTAFGDVVAQLGAYFAGELTTFELELDLAGTAFQRR
VWEALLTIPYGETRSYGDIARQIGAPGAFRAVGLANGHNPIGIIVPCHRVIGANGSLTGYGGGIERKRMLLGMERQFHSA
VPTLFD
>Mature_165_residues
TIRFRTVDSPVGLLTLAGRDDRLRHLRMVDQTYEPSRQGWEPDPTAFGDVVAQLGAYFAGELTTFELELDLAGTAFQRRV
WEALLTIPYGETRSYGDIARQIGAPGAFRAVGLANGHNPIGIIVPCHRVIGANGSLTGYGGGIERKRMLLGMERQFHSAV
PTLFD

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=166, Percent_Identity=31.3253012048193, Blast_Score=78, Evalue=3e-15,
Organism=Escherichia coli, GI1787596, Length=132, Percent_Identity=49.2424242424242, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI1788542, Length=112, Percent_Identity=46.4285714285714, Blast_Score=100, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI115533068, Length=80, Percent_Identity=57.5, Blast_Score=94, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI115533070, Length=80, Percent_Identity=57.5, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6320001, Length=85, Percent_Identity=48.2352941176471, Blast_Score=95, Evalue=6e-21,
Organism=Drosophila melanogaster, GI17137554, Length=126, Percent_Identity=32.5396825396825, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18217; Mature: 18086

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIRFRTVDSPVGLLTLAGRDDRLRHLRMVDQTYEPSRQGWEPDPTAFGDVVAQLGAYFA
CEEEEEECCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHC
GELTTFELELDLAGTAFQRRVWEALLTIPYGETRSYGDIARQIGAPGAFRAVGLANGHNP
CCEEEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCC
IGIIVPCHRVIGANGSLTGYGGGIERKRMLLGMERQFHSAVPTLFD
EEEEEECHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TIRFRTVDSPVGLLTLAGRDDRLRHLRMVDQTYEPSRQGWEPDPTAFGDVVAQLGAYFA
EEEEEECCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHC
GELTTFELELDLAGTAFQRRVWEALLTIPYGETRSYGDIARQIGAPGAFRAVGLANGHNP
CCEEEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCC
IGIIVPCHRVIGANGSLTGYGGGIERKRMLLGMERQFHSAVPTLFD
EEEEEECHHHHCCCCCEECCCCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA