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The map label for this gene is echA14 [H]

Identifier: 119869728

GI number: 119869728

Start: 3902547

End: 3903299

Strand: Direct

Name: echA14 [H]

Synonym: Mkms_3696

Alternate gene names: 119869728

Gene position: 3902547-3903299 (Clockwise)

Preceding gene: 119869727

Following gene: 119869737

Centisome position: 68.02

GC content: 73.17

Gene sequence:

>753_bases
GTGGCTGATTCCGTACTGATGCAGGTCGACGACCACGTCGCCCTGGTCACCGTCAACGATCCCGACCGTCGCAACGCGGT
GACCTTCGAGATGTCGGCGGCCCTGCGCGCAGCCGTCGACGCGGCGGAGGCCGACCCCGAGGTGCACGCGCTGATCGTCA
CCGGGGCAGGCAAGGCGTTCTGCGCGGGCGCGGATCTGACCGCCCTCGGCGAGGCCACCGAGGACGGTCTGCGCCGCATC
TACGACGGCTTCCTCGCGGTGGCGCACTGCCCGCTGCCGACCATCGCGGCGGTGAACGGGGCGGCCGTCGGCGCCGGCCT
CAACCTGGCGCTCGCGGCGGACGTCCGGATCGCAGGCCCGAATGCCCTCTTCGATCCGAGGTTCCAGAAGCTCGGCATCC
ACCCCGGTGGCGGGGCGACCTGGATGCTGCAGCGGGCGGTGGGTCCGGAGGTGGCGCGCGCGGCGCTGTTGTTCGGGATG
CGCTTCGACGCCGAGGCCGCGGTCCGCCACGGCCTCGCCCTCGAGGTCGCCGACGATCCCGTCGCCACCGCACGTCAGTT
GGCCGCCGGGCCTGCGGCCGCACCGCGCGAGGTGGTGCTGGCGACGAAGGCGTCGATGCGCGCGACCGCCAGCCCCGGCA
CGGTCGACACCGAACAGCACCGCACGGCGGTCGACATCGAACTCGGTCCTCAGGCGGCGTCCATCGAATCGCCGGAGTTC
GCCCGCCGCCTCGCCGCGGCCAAACGCAAGTAG

Upstream 100 bases:

>100_bases
CATGACATTTGGACCACGGTGCCGCAGGTCCACACCTTAGATCGACCGCTGTCCGATGCCCGTCACCGGGGCCGACGACG
GGCCCGAATAGGGTGATCGC

Downstream 100 bases:

>100_bases
GTCACAGGTCGAGCAGCGCGAGCTCCGGCGCTTCGACGAGCTCGCGCAACTCGCCCAGGAAGGCCGCCACGGTGGCCCCG
TCGGCGATGCGATGGTCGAA

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MADSVLMQVDDHVALVTVNDPDRRNAVTFEMSAALRAAVDAAEADPEVHALIVTGAGKAFCAGADLTALGEATEDGLRRI
YDGFLAVAHCPLPTIAAVNGAAVGAGLNLALAADVRIAGPNALFDPRFQKLGIHPGGGATWMLQRAVGPEVARAALLFGM
RFDAEAAVRHGLALEVADDPVATARQLAAGPAAAPREVVLATKASMRATASPGTVDTEQHRTAVDIELGPQAASIESPEF
ARRLAAAKRK

Sequences:

>Translated_250_residues
MADSVLMQVDDHVALVTVNDPDRRNAVTFEMSAALRAAVDAAEADPEVHALIVTGAGKAFCAGADLTALGEATEDGLRRI
YDGFLAVAHCPLPTIAAVNGAAVGAGLNLALAADVRIAGPNALFDPRFQKLGIHPGGGATWMLQRAVGPEVARAALLFGM
RFDAEAAVRHGLALEVADDPVATARQLAAGPAAAPREVVLATKASMRATASPGTVDTEQHRTAVDIELGPQAASIESPEF
ARRLAAAKRK
>Mature_249_residues
ADSVLMQVDDHVALVTVNDPDRRNAVTFEMSAALRAAVDAAEADPEVHALIVTGAGKAFCAGADLTALGEATEDGLRRIY
DGFLAVAHCPLPTIAAVNGAAVGAGLNLALAADVRIAGPNALFDPRFQKLGIHPGGGATWMLQRAVGPEVARAALLFGMR
FDAEAAVRHGLALEVADDPVATARQLAAGPAAAPREVVLATKASMRATASPGTVDTEQHRTAVDIELGPQAASIESPEFA
RRLAAAKRK

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=208, Percent_Identity=34.1346153846154, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI37594469, Length=186, Percent_Identity=30.1075268817204, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI37594471, Length=186, Percent_Identity=30.1075268817204, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI157694516, Length=211, Percent_Identity=28.436018957346, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI213417737, Length=211, Percent_Identity=28.436018957346, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI260274832, Length=188, Percent_Identity=28.7234042553192, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI45643119, Length=188, Percent_Identity=28.7234042553192, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI260275230, Length=188, Percent_Identity=28.7234042553192, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI70995211, Length=184, Percent_Identity=27.7173913043478, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI4502327, Length=179, Percent_Identity=33.5195530726257, Blast_Score=64, Evalue=9e-11,
Organism=Escherichia coli, GI1787660, Length=261, Percent_Identity=35.2490421455939, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787659, Length=206, Percent_Identity=36.4077669902913, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1788597, Length=169, Percent_Identity=31.9526627218935, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI221142681, Length=221, Percent_Identity=32.579185520362, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1788682, Length=196, Percent_Identity=29.5918367346939, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790281, Length=217, Percent_Identity=29.0322580645161, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17536985, Length=187, Percent_Identity=29.9465240641711, Blast_Score=96, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI25145438, Length=168, Percent_Identity=35.7142857142857, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17554946, Length=206, Percent_Identity=33.0097087378641, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17540714, Length=188, Percent_Identity=29.7872340425532, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17549921, Length=178, Percent_Identity=31.4606741573034, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17560910, Length=247, Percent_Identity=26.3157894736842, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=186, Percent_Identity=29.0322580645161, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=224, Percent_Identity=32.5892857142857, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=178, Percent_Identity=28.6516853932584, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI20129971, Length=181, Percent_Identity=34.2541436464088, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24653477, Length=181, Percent_Identity=34.2541436464088, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI19920382, Length=174, Percent_Identity=28.735632183908, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24650670, Length=180, Percent_Identity=30.5555555555556, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24653139, Length=166, Percent_Identity=29.5180722891566, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28571729, Length=153, Percent_Identity=28.1045751633987, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28571730, Length=153, Percent_Identity=28.1045751633987, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 25753; Mature: 25621

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADSVLMQVDDHVALVTVNDPDRRNAVTFEMSAALRAAVDAAEADPEVHALIVTGAGKAF
CCCCEEEEECCCEEEEEECCCCCCCEEEEEHHHHHHHHHHHHCCCCCEEEEEEECCCCEE
CAGADLTALGEATEDGLRRIYDGFLAVAHCPLPTIAAVNGAAVGAGLNLALAADVRIAGP
ECCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCEECCCCEEEEEEEEEEECC
NALFDPRFQKLGIHPGGGATWMLQRAVGPEVARAALLFGMRFDAEAAVRHGLALEVADDP
CCCCCCCHHHCCCCCCCCHHEEEEHHCCHHHHHHHHHHHCCCCHHHHHHCCEEEEECCCC
VATARQLAAGPAAAPREVVLATKASMRATASPGTVDTEQHRTAVDIELGPQAASIESPEF
HHHHHHHHCCCCCCCCEEEEEECCCCEECCCCCCCCCCCCCEEEEEEECCCCCCCCCHHH
ARRLAAAKRK
HHHHHHHCCC
>Mature Secondary Structure 
ADSVLMQVDDHVALVTVNDPDRRNAVTFEMSAALRAAVDAAEADPEVHALIVTGAGKAF
CCCEEEEECCCEEEEEECCCCCCCEEEEEHHHHHHHHHHHHCCCCCEEEEEEECCCCEE
CAGADLTALGEATEDGLRRIYDGFLAVAHCPLPTIAAVNGAAVGAGLNLALAADVRIAGP
ECCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCEECCCCEEEEEEEEEEECC
NALFDPRFQKLGIHPGGGATWMLQRAVGPEVARAALLFGMRFDAEAAVRHGLALEVADDP
CCCCCCCHHHCCCCCCCCHHEEEEHHCCHHHHHHHHHHHCCCCHHHHHHCCEEEEECCCC
VATARQLAAGPAAAPREVVLATKASMRATASPGTVDTEQHRTAVDIELGPQAASIESPEF
HHHHHHHHCCCCCCCCEEEEEECCCCEECCCCCCCCCCCCCEEEEEEECCCCCCCCCHHH
ARRLAAAKRK
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]