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The map label for this gene is amiA2 [H]

Identifier: 119869546

GI number: 119869546

Start: 3689994

End: 3691508

Strand: Direct

Name: amiA2 [H]

Synonym: Mkms_3514

Alternate gene names: 119869546

Gene position: 3689994-3691508 (Clockwise)

Preceding gene: 119869542

Following gene: 119869552

Centisome position: 64.32

GC content: 72.54

Gene sequence:

>1515_bases
ATGGCCGATTCGTCTGCTCCCCGCCCCGCTCGAACCGCCTTCCCGACCCTGACCGACCAGCTCTATCAGCTCGCCAGTGG
CGCGGTGACGGCGGACGATCTGGTGCGCCGGTCCCTGCACGCGATCTCGGCGAGCCAGCCGACTCTCAACGCGTTCCGGG
TGGTGCTGGCCGAACAGGCCCTCGCCGAGGCCGCCGAGGCCGACCGCCGGCGCGCCGCCGGGGATCAGCGTCCGCTGCTC
GGCATCCCGATCGCGGTGAAAGACGACGTCGACATCGCCGGGGTGCCGACGCGCTTCGGGACCGCAGGCACGGTGCGGCC
CGCCGACGCCGACGCCGAGGTGGTCCGGCGCCTGCGCGCCGCCGGTGCGGTGATCGTCGGCAAGACCAACACCTGCGAAC
TCGGCCAGTGGCCGTTCACCAGCGGACCGGCCTTCGGCCACACCCGCAACCCGTGGTCGCGCGAGCACACCCCCGGCGGA
TCGTCGGGCGGCAGCGCCGCGGCGGTCGCGGCCGGCCTGGTCACCGCGGCGATCGGCTCCGACGGCGCAGGCAGTGTGCG
CATCCCGGCGGCCTGGTGCCATCTCGTCGGCATCAAGCCGCAGCGCGGCCGCATCTCGACCTCGCCGCTGGCCGAGGCGT
TCAACGGCATCACGGTCAACGGGGTGCTCGCCCGCACCGTCACCGATGCGGCGCTGGTGCTCGATGCGGCGTCGGGCAAC
GCGGCAGGCGACCGGCACCGGCCCGCACCGGTCCGGGTGTCGGAGTACGTCGCCCGCGCGCCGGGACCGCTGCGGATCGG
GTTGTCGACGAAGTTCCCGTTCACCGGCTTCCACGCCACCCTGCACCCCGACATCCGCGCGGCGCTGCACACCGTCTCGG
GCCATCTGGAGGAACTGGGCCACACCGTGCGGCCCGCGGACCCCGACTACACGGTCGGCATGTCGTGGAACTTCCTGTCC
CGCTCCACCTCTGGGCTGTTGGACTGGGCCGAGCGGCTCGGCTACGGCGTCGACTTCGACAAGCGCACGAAGGCGAATCT
GCGGATGGGCCGGCTGCTGTCCGAACACGTCCTGCGCAAGGCGCGCGCCAAGGAGGCCGCCGCGCAGCGCCGAATCGGCT
GGGTGTTCAACATCTGCGACGTGGTGCTGGCCCCGACCACCGCGCAGCCACCGCCCCGCGTGCACGACTTCGACGAGCTC
GGCGGACTGGCCACCGACCGCACGATGATCAAGGCGTGTCCGGTCACCTGGCCGTGGAACCTGCTCGGCTGGCCGTCGAT
CAACGTCCCGGCCGGGTTCACCGCCGACGGACTGCCGATCGGCGTGCAGCTCATGGGTCCCGCCGAGAGCGAACCGCTGC
TCATCTCGCTGGCCGCCGAACTCGAGGCGGTCAGCGGGTGGGTCGCCCGTCAGCCCAAGGTGTGGTGGGATACGCCGCAG
ATGTCGCAGGCCGCGAAGGCCCCGACGGACGACGGACCGTCCGGCGACCCGCTGGAACTGCGCGAGACGGCTTAA

Upstream 100 bases:

>100_bases
GGCGCAGCACGACCGCGCGGTCCCGGTACAGCCGCATCAGACCAGTGTTCCATCACGGTCCGACAATGCTCGGTTGGCAG
CGCCGATATGGTTGGAACCG

Downstream 100 bases:

>100_bases
AAGCCCAGTCGGCCAAGCTGTTTGGGGTCGCGCTGCCAGTTCTTGGCGATCTTCACGCGCAGGTCGAGGTAGACCTTCGT
GCCGAGCAGCTTCTCGATCT

Product: amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MADSSAPRPARTAFPTLTDQLYQLASGAVTADDLVRRSLHAISASQPTLNAFRVVLAEQALAEAAEADRRRAAGDQRPLL
GIPIAVKDDVDIAGVPTRFGTAGTVRPADADAEVVRRLRAAGAVIVGKTNTCELGQWPFTSGPAFGHTRNPWSREHTPGG
SSGGSAAAVAAGLVTAAIGSDGAGSVRIPAAWCHLVGIKPQRGRISTSPLAEAFNGITVNGVLARTVTDAALVLDAASGN
AAGDRHRPAPVRVSEYVARAPGPLRIGLSTKFPFTGFHATLHPDIRAALHTVSGHLEELGHTVRPADPDYTVGMSWNFLS
RSTSGLLDWAERLGYGVDFDKRTKANLRMGRLLSEHVLRKARAKEAAAQRRIGWVFNICDVVLAPTTAQPPPRVHDFDEL
GGLATDRTMIKACPVTWPWNLLGWPSINVPAGFTADGLPIGVQLMGPAESEPLLISLAAELEAVSGWVARQPKVWWDTPQ
MSQAAKAPTDDGPSGDPLELRETA

Sequences:

>Translated_504_residues
MADSSAPRPARTAFPTLTDQLYQLASGAVTADDLVRRSLHAISASQPTLNAFRVVLAEQALAEAAEADRRRAAGDQRPLL
GIPIAVKDDVDIAGVPTRFGTAGTVRPADADAEVVRRLRAAGAVIVGKTNTCELGQWPFTSGPAFGHTRNPWSREHTPGG
SSGGSAAAVAAGLVTAAIGSDGAGSVRIPAAWCHLVGIKPQRGRISTSPLAEAFNGITVNGVLARTVTDAALVLDAASGN
AAGDRHRPAPVRVSEYVARAPGPLRIGLSTKFPFTGFHATLHPDIRAALHTVSGHLEELGHTVRPADPDYTVGMSWNFLS
RSTSGLLDWAERLGYGVDFDKRTKANLRMGRLLSEHVLRKARAKEAAAQRRIGWVFNICDVVLAPTTAQPPPRVHDFDEL
GGLATDRTMIKACPVTWPWNLLGWPSINVPAGFTADGLPIGVQLMGPAESEPLLISLAAELEAVSGWVARQPKVWWDTPQ
MSQAAKAPTDDGPSGDPLELRETA
>Mature_503_residues
ADSSAPRPARTAFPTLTDQLYQLASGAVTADDLVRRSLHAISASQPTLNAFRVVLAEQALAEAAEADRRRAAGDQRPLLG
IPIAVKDDVDIAGVPTRFGTAGTVRPADADAEVVRRLRAAGAVIVGKTNTCELGQWPFTSGPAFGHTRNPWSREHTPGGS
SGGSAAAVAAGLVTAAIGSDGAGSVRIPAAWCHLVGIKPQRGRISTSPLAEAFNGITVNGVLARTVTDAALVLDAASGNA
AGDRHRPAPVRVSEYVARAPGPLRIGLSTKFPFTGFHATLHPDIRAALHTVSGHLEELGHTVRPADPDYTVGMSWNFLSR
STSGLLDWAERLGYGVDFDKRTKANLRMGRLLSEHVLRKARAKEAAAQRRIGWVFNICDVVLAPTTAQPPPRVHDFDELG
GLATDRTMIKACPVTWPWNLLGWPSINVPAGFTADGLPIGVQLMGPAESEPLLISLAAELEAVSGWVARQPKVWWDTPQM
SQAAKAPTDDGPSGDPLELRETA

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=500, Percent_Identity=27.6, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI195972892, Length=254, Percent_Identity=31.496062992126, Blast_Score=108, Evalue=2e-23,
Organism=Homo sapiens, GI166795287, Length=242, Percent_Identity=31.8181818181818, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17556264, Length=366, Percent_Identity=28.6885245901639, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17537465, Length=515, Percent_Identity=24.0776699029126, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17538252, Length=530, Percent_Identity=26.2264150943396, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71990152, Length=251, Percent_Identity=30.6772908366534, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17543272, Length=423, Percent_Identity=27.6595744680851, Blast_Score=95, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17556276, Length=262, Percent_Identity=30.9160305343511, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=262, Percent_Identity=30.9160305343511, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17538254, Length=296, Percent_Identity=29.7297297297297, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI32565337, Length=249, Percent_Identity=29.718875502008, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319685, Length=197, Percent_Identity=36.5482233502538, Blast_Score=103, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6320448, Length=213, Percent_Identity=30.5164319248826, Blast_Score=74, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6323950, Length=457, Percent_Identity=23.4135667396061, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24648113, Length=502, Percent_Identity=30.6772908366534, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24644968, Length=513, Percent_Identity=26.1208576998051, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI161078093, Length=516, Percent_Identity=26.7441860465116, Blast_Score=122, Evalue=8e-28,
Organism=Drosophila melanogaster, GI21356731, Length=478, Percent_Identity=25.1046025104602, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24652985, Length=502, Percent_Identity=25.0996015936255, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19922090, Length=502, Percent_Identity=25.0996015936255, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24652981, Length=502, Percent_Identity=25.0996015936255, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24652983, Length=502, Percent_Identity=25.0996015936255, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI45550774, Length=197, Percent_Identity=33.502538071066, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648435, Length=197, Percent_Identity=33.502538071066, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648437, Length=197, Percent_Identity=33.502538071066, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24648441, Length=140, Percent_Identity=37.8571428571429, Blast_Score=86, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24648439, Length=140, Percent_Identity=37.8571428571429, Blast_Score=86, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 53286; Mature: 53155

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADSSAPRPARTAFPTLTDQLYQLASGAVTADDLVRRSLHAISASQPTLNAFRVVLAEQA
CCCCCCCCCCHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LAEAAEADRRRAAGDQRPLLGIPIAVKDDVDIAGVPTRFGTAGTVRPADADAEVVRRLRA
HHHHHHHHHHHHCCCCCCEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
AGAVIVGKTNTCELGQWPFTSGPAFGHTRNPWSREHTPGGSSGGSAAAVAAGLVTAAIGS
CCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
DGAGSVRIPAAWCHLVGIKPQRGRISTSPLAEAFNGITVNGVLARTVTDAALVLDAASGN
CCCCCEECCHHHEEEECCCCCCCCCCCCHHHHHHCCEEECCCHHHHHHCEEEEEECCCCC
AAGDRHRPAPVRVSEYVARAPGPLRIGLSTKFPFTGFHATLHPDIRAALHTVSGHLEELG
CCCCCCCCCCCCHHHHHHCCCCCEEEECCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCC
HTVRPADPDYTVGMSWNFLSRSTSGLLDWAERLGYGVDFDKRTKANLRMGRLLSEHVLRK
CCCCCCCCCEEEEEEEHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ARAKEAAAQRRIGWVFNICDVVLAPTTAQPPPRVHDFDELGGLATDRTMIKACPVTWPWN
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCHHHHCCCCCCCCC
LLGWPSINVPAGFTADGLPIGVQLMGPAESEPLLISLAAELEAVSGWVARQPKVWWDTPQ
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEHHHHHHHHHHHHHCCCCCEECCCC
MSQAAKAPTDDGPSGDPLELRETA
HHHHHCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ADSSAPRPARTAFPTLTDQLYQLASGAVTADDLVRRSLHAISASQPTLNAFRVVLAEQA
CCCCCCCCCHHHCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
LAEAAEADRRRAAGDQRPLLGIPIAVKDDVDIAGVPTRFGTAGTVRPADADAEVVRRLRA
HHHHHHHHHHHHCCCCCCEEECCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
AGAVIVGKTNTCELGQWPFTSGPAFGHTRNPWSREHTPGGSSGGSAAAVAAGLVTAAIGS
CCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
DGAGSVRIPAAWCHLVGIKPQRGRISTSPLAEAFNGITVNGVLARTVTDAALVLDAASGN
CCCCCEECCHHHEEEECCCCCCCCCCCCHHHHHHCCEEECCCHHHHHHCEEEEEECCCCC
AAGDRHRPAPVRVSEYVARAPGPLRIGLSTKFPFTGFHATLHPDIRAALHTVSGHLEELG
CCCCCCCCCCCCHHHHHHCCCCCEEEECCCCCCCCCCEEEECHHHHHHHHHHHHHHHHCC
HTVRPADPDYTVGMSWNFLSRSTSGLLDWAERLGYGVDFDKRTKANLRMGRLLSEHVLRK
CCCCCCCCCEEEEEEEHHHHCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
ARAKEAAAQRRIGWVFNICDVVLAPTTAQPPPRVHDFDELGGLATDRTMIKACPVTWPWN
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCHHHHCCCCCCCCC
LLGWPSINVPAGFTADGLPIGVQLMGPAESEPLLISLAAELEAVSGWVARQPKVWWDTPQ
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEHHHHHHHHHHHHHCCCCCEECCCC
MSQAAKAPTDDGPSGDPLELRETA
HHHHHCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]