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The map label for this gene is lipA

Identifier: 119869410

GI number: 119869410

Start: 3541868

End: 3542845

Strand: Direct

Name: lipA

Synonym: Mkms_3378

Alternate gene names: 119869410

Gene position: 3541868-3542845 (Clockwise)

Preceding gene: 119869409

Following gene: 119869411

Centisome position: 61.73

GC content: 68.1

Gene sequence:

>978_bases
GTGACCGTCACCCCTTCGGGATCCAATGGCGCCGGCTCCGCGGCTCCGGAAGGCCGCAAGCTGCTGCGTCTCGAGGTCCG
TAACGCCCAGACGCCGATCGAGCGCAAGCCGCCGTGGATCAAGACCCGCGCCAGGATGGGCCCGGAGTACAAAGAGCTCA
AATCGCTGGTCAAGCGCGAGGGCCTGCACACCGTGTGCGAAGAGGCCGGCTGCCCCAACATCTTCGAATGCTGGGAGGAC
CGCGAGGCCACCTTCCTGATCGGCGGCGAGCAGTGCACCCGGCGCTGCGACTTCTGCCAGATCGACACCGGCAAGCCGTC
AGAACTCGACCGCGACGAACCTCGCCGGGTCGCCGAGAGCGTTCAGGCCATGGGTCTGCGGTACTCGACGGTGACGGGTG
TGGCCCGCGACGATCTGCCCGACGGCGGCGCCTGGCTCTACGCCGAGACGGTGCGCGAGATCAAGCGACTCAACCCCAAC
ACCGGCGTCGAGTTGCTGATCCCCGATTTCAACGGAGACCCGGCGCTGCTGGCGCAGGTCTTCGAGTCGCGCCCAGAAGT
GTTGGCGCACAACGTCGAAACGGTGCCGCGCATCTTCAAGCGGATCCGGCCGGCCTTCCGCTACGAACGCAGCCTCGCGG
TGTTGACCGCGGCCCGCGACGACAACCTGGTCACCAAGAGCAACCTGATCCTCGGCATGGGTGAGACGCCCGACGAGGTG
CGCACCGCGCTGGTCGACCTGCACGAGGCGGGCTGCGACATCATCACGATCACGCAGTATCTGCGGCCCTCGCCGCGCCA
CCATCCGGTGGAACGCTGGGTGAAACCGGAGGAGTTCGTCGAGTTCGCGCAGTTCGCCGAAGGGCTCGGATTCGCCGGGG
TGCTGTCCGGTCCGCTGGTGCGGTCGTCGTACCGGGCGGGGCGGCTGTACGCGCAGGCCGCGCGGCTGAAACCGGCCGCG
ACGCCACCCGTATCCTGA

Upstream 100 bases:

>100_bases
CCGACGCCGTCGCCGCGCGGGTGTGTGACGCGCTCGACGGGAGGCTGGCCGTGTCCGGCGTATCCGTTGACACCTACGCC
TCCGGCGTAGCATCGACACA

Downstream 100 bases:

>100_bases
GGGTATGGCCAAGACCCGCAACACCGCCGCCACCAAGGCCGCCAAGGCCGAGGCGAAGGCCGCCCGCAAGGCCGCGTCCA
AGCAGCGGCGCAGTCAGCTC

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MTVTPSGSNGAGSAAPEGRKLLRLEVRNAQTPIERKPPWIKTRARMGPEYKELKSLVKREGLHTVCEEAGCPNIFECWED
REATFLIGGEQCTRRCDFCQIDTGKPSELDRDEPRRVAESVQAMGLRYSTVTGVARDDLPDGGAWLYAETVREIKRLNPN
TGVELLIPDFNGDPALLAQVFESRPEVLAHNVETVPRIFKRIRPAFRYERSLAVLTAARDDNLVTKSNLILGMGETPDEV
RTALVDLHEAGCDIITITQYLRPSPRHHPVERWVKPEEFVEFAQFAEGLGFAGVLSGPLVRSSYRAGRLYAQAARLKPAA
TPPVS

Sequences:

>Translated_325_residues
MTVTPSGSNGAGSAAPEGRKLLRLEVRNAQTPIERKPPWIKTRARMGPEYKELKSLVKREGLHTVCEEAGCPNIFECWED
REATFLIGGEQCTRRCDFCQIDTGKPSELDRDEPRRVAESVQAMGLRYSTVTGVARDDLPDGGAWLYAETVREIKRLNPN
TGVELLIPDFNGDPALLAQVFESRPEVLAHNVETVPRIFKRIRPAFRYERSLAVLTAARDDNLVTKSNLILGMGETPDEV
RTALVDLHEAGCDIITITQYLRPSPRHHPVERWVKPEEFVEFAQFAEGLGFAGVLSGPLVRSSYRAGRLYAQAARLKPAA
TPPVS
>Mature_324_residues
TVTPSGSNGAGSAAPEGRKLLRLEVRNAQTPIERKPPWIKTRARMGPEYKELKSLVKREGLHTVCEEAGCPNIFECWEDR
EATFLIGGEQCTRRCDFCQIDTGKPSELDRDEPRRVAESVQAMGLRYSTVTGVARDDLPDGGAWLYAETVREIKRLNPNT
GVELLIPDFNGDPALLAQVFESRPEVLAHNVETVPRIFKRIRPAFRYERSLAVLTAARDDNLVTKSNLILGMGETPDEVR
TALVDLHEAGCDIITITQYLRPSPRHHPVERWVKPEEFVEFAQFAEGLGFAGVLSGPLVRSSYRAGRLYAQAARLKPAAT
PPVS

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=287, Percent_Identity=42.8571428571429, Blast_Score=234, Evalue=9e-62,
Organism=Homo sapiens, GI37577164, Length=246, Percent_Identity=42.6829268292683, Blast_Score=196, Evalue=3e-50,
Organism=Escherichia coli, GI1786846, Length=308, Percent_Identity=39.2857142857143, Blast_Score=229, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI32564533, Length=290, Percent_Identity=40.3448275862069, Blast_Score=216, Evalue=1e-56,
Organism=Saccharomyces cerevisiae, GI6324770, Length=285, Percent_Identity=43.859649122807, Blast_Score=225, Evalue=6e-60,
Organism=Drosophila melanogaster, GI221513272, Length=285, Percent_Identity=44.9122807017544, Blast_Score=240, Evalue=7e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_MYCSJ (A3Q1S8)

Other databases:

- EMBL:   CP000580
- RefSeq:   YP_001071596.1
- ProteinModelPortal:   A3Q1S8
- STRING:   A3Q1S8
- EnsemblBacteria:   EBMYCT00000047869
- GeneID:   4879039
- GenomeReviews:   CP000580_GR
- KEGG:   mjl:Mjls_3327
- eggNOG:   COG0320
- GeneTree:   EBGT00050000017436
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- ProtClustDB:   PRK05481
- BioCyc:   MSP164757:MJLS_3327-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 36148; Mature: 36016

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTPSGSNGAGSAAPEGRKLLRLEVRNAQTPIERKPPWIKTRARMGPEYKELKSLVKRE
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHC
GLHTVCEEAGCPNIFECWEDREATFLIGGEQCTRRCDFCQIDTGKPSELDRDEPRRVAES
CHHHHHHHCCCCCHHHHHCCCCCEEEECCHHHHCCCCEEECCCCCCCCCCCCHHHHHHHH
VQAMGLRYSTVTGVARDDLPDGGAWLYAETVREIKRLNPNTGVELLIPDFNGDPALLAQV
HHHHCCCHHHHHCCHHCCCCCCCEEHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHH
FESRPEVLAHNVETVPRIFKRIRPAFRYERSLAVLTAARDDNLVTKSNLILGMGETPDEV
HHCCHHHHHHCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEECCEEEECCCCHHHH
RTALVDLHEAGCDIITITQYLRPSPRHHPVERWVKPEEFVEFAQFAEGLGFAGVLSGPLV
HHHHHHHHHCCCCEEEHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCHHHHCCHHH
RSSYRAGRLYAQAARLKPAATPPVS
HHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TVTPSGSNGAGSAAPEGRKLLRLEVRNAQTPIERKPPWIKTRARMGPEYKELKSLVKRE
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHHHC
GLHTVCEEAGCPNIFECWEDREATFLIGGEQCTRRCDFCQIDTGKPSELDRDEPRRVAES
CHHHHHHHCCCCCHHHHHCCCCCEEEECCHHHHCCCCEEECCCCCCCCCCCCHHHHHHHH
VQAMGLRYSTVTGVARDDLPDGGAWLYAETVREIKRLNPNTGVELLIPDFNGDPALLAQV
HHHHCCCHHHHHCCHHCCCCCCCEEHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHH
FESRPEVLAHNVETVPRIFKRIRPAFRYERSLAVLTAARDDNLVTKSNLILGMGETPDEV
HHCCHHHHHHCHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEEECCEEEECCCCHHHH
RTALVDLHEAGCDIITITQYLRPSPRHHPVERWVKPEEFVEFAQFAEGLGFAGVLSGPLV
HHHHHHHHHCCCCEEEHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHCCCCHHHHCCHHH
RSSYRAGRLYAQAARLKPAATPPVS
HHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA