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The map label for this gene is lipB

Identifier: 119869409

GI number: 119869409

Start: 3541155

End: 3541871

Strand: Direct

Name: lipB

Synonym: Mkms_3377

Alternate gene names: 119869409

Gene position: 3541155-3541871 (Clockwise)

Preceding gene: 119869408

Following gene: 119869410

Centisome position: 61.72

GC content: 71.83

Gene sequence:

>717_bases
GTGTCCATGGCGATTTCGATCAGGTCGTCGACCCGTCCGGTCGAGGTGCGGCGACTGGGCACCGTCGAGTACCTCGACGC
GTGGGAGCTGCAGCGCGGCCTCGTCGACGCGCGGGTGGCCGGCGGCTCGGATGCGCTTCTGCTGCTTCAGCATCCGTCGG
TCTACACGGCCGGTAAGCGCACCGAACCGCACGAACGCCCGGCCGACGGCACCCCGGTGGTGGACACCGATCGCGGCGGC
AAGATCACCTGGCACGGCCCCGGCCAGCTGGTCGGCTATCCGATCGTCGGGCTGGCCGAACCGCTCGACGTGGTGAACTT
CGTGCGCCGCATCGAGGAGGCGCTGATCGCGGTGTGCACCGGCTTGGGCCTCGACGCCGGGCGGGTCGAGGGCCGGTCGG
GGGTCTGGCTGCCGGGCGACGGCCTCCGGCCGGAACGCAAGATCGGCGCCATCGGCATCCGGGTGTCCCGCGGCACCACG
CTGCACGGGTTCGCCCTCAACTGCGACTGCGACCTGTCGGCGTTCTCGGCCATCGTGCCGTGCGGGATCGCCGACGCCGG
CGTGACGTCGCTGACCGCGGAACTGGGCCGCCGCGTCACCGTCGACGAGGTCACCGACGCCGTCGCCGCGCGGGTGTGTG
ACGCGCTCGACGGGAGGCTGGCCGTGTCCGGCGTATCCGTTGACACCTACGCCTCCGGCGTAGCATCGACACAGTGA

Upstream 100 bases:

>100_bases
CCGCCGCGCTGGAGCGGGCCGGGTTCACGTTCCACCACAACACCGTCGGCGAAGCGCTCGCCTACGCCACCGGCCCGCGC
GACGCCTGACGAGTAGCGTC

Downstream 100 bases:

>100_bases
CCGTCACCCCTTCGGGATCCAATGGCGCCGGCTCCGCGGCTCCGGAAGGCCGCAAGCTGCTGCGTCTCGAGGTCCGTAAC
GCCCAGACGCCGATCGAGCG

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MSMAISIRSSTRPVEVRRLGTVEYLDAWELQRGLVDARVAGGSDALLLLQHPSVYTAGKRTEPHERPADGTPVVDTDRGG
KITWHGPGQLVGYPIVGLAEPLDVVNFVRRIEEALIAVCTGLGLDAGRVEGRSGVWLPGDGLRPERKIGAIGIRVSRGTT
LHGFALNCDCDLSAFSAIVPCGIADAGVTSLTAELGRRVTVDEVTDAVAARVCDALDGRLAVSGVSVDTYASGVASTQ

Sequences:

>Translated_238_residues
MSMAISIRSSTRPVEVRRLGTVEYLDAWELQRGLVDARVAGGSDALLLLQHPSVYTAGKRTEPHERPADGTPVVDTDRGG
KITWHGPGQLVGYPIVGLAEPLDVVNFVRRIEEALIAVCTGLGLDAGRVEGRSGVWLPGDGLRPERKIGAIGIRVSRGTT
LHGFALNCDCDLSAFSAIVPCGIADAGVTSLTAELGRRVTVDEVTDAVAARVCDALDGRLAVSGVSVDTYASGVASTQ
>Mature_237_residues
SMAISIRSSTRPVEVRRLGTVEYLDAWELQRGLVDARVAGGSDALLLLQHPSVYTAGKRTEPHERPADGTPVVDTDRGGK
ITWHGPGQLVGYPIVGLAEPLDVVNFVRRIEEALIAVCTGLGLDAGRVEGRSGVWLPGDGLRPERKIGAIGIRVSRGTTL
HGFALNCDCDLSAFSAIVPCGIADAGVTSLTAELGRRVTVDEVTDAVAARVCDALDGRLAVSGVSVDTYASGVASTQ

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=209, Percent_Identity=41.1483253588517, Blast_Score=103, Evalue=2e-22,
Organism=Escherichia coli, GI87081767, Length=204, Percent_Identity=31.3725490196078, Blast_Score=99, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323268, Length=193, Percent_Identity=27.979274611399, Blast_Score=73, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21355483, Length=222, Percent_Identity=37.3873873873874, Blast_Score=123, Evalue=9e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_MYCSJ (A3Q1S7)

Other databases:

- EMBL:   CP000580
- RefSeq:   YP_001071595.1
- ProteinModelPortal:   A3Q1S7
- SMR:   A3Q1S7
- STRING:   A3Q1S7
- EnsemblBacteria:   EBMYCT00000050581
- GeneID:   4879038
- GenomeReviews:   CP000580_GR
- KEGG:   mjl:Mjls_3326
- eggNOG:   COG0321
- GeneTree:   EBGT00050000016282
- HOGENOM:   HBG716160
- OMA:   HPPVFTL
- PhylomeDB:   A3Q1S7
- ProtClustDB:   PRK14345
- BioCyc:   MSP164757:MJLS_3326-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 24956; Mature: 24824

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 181-181

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMAISIRSSTRPVEVRRLGTVEYLDAWELQRGLVDARVAGGSDALLLLQHPSVYTAGKR
CCEEEEECCCCCCEEEEECCCHHHHHHHHHHHCCCEEEECCCCCEEEEEECCCEEECCCC
TEPHERPADGTPVVDTDRGGKITWHGPGQLVGYPIVGLAEPLDVVNFVRRIEEALIAVCT
CCCCCCCCCCCCEEECCCCCEEEECCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
GLGLDAGRVEGRSGVWLPGDGLRPERKIGAIGIRVSRGTTLHGFALNCDCDLSAFSAIVP
CCCCCCCCCCCCCCEEECCCCCCCCCCEEEEEEEEECCCEEEEEEEECCCCHHHHHHHHC
CGIADAGVTSLTAELGRRVTVDEVTDAVAARVCDALDGRLAVSGVSVDTYASGVASTQ
CCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCCC
>Mature Secondary Structure 
SMAISIRSSTRPVEVRRLGTVEYLDAWELQRGLVDARVAGGSDALLLLQHPSVYTAGKR
CEEEEECCCCCCEEEEECCCHHHHHHHHHHHCCCEEEECCCCCEEEEEECCCEEECCCC
TEPHERPADGTPVVDTDRGGKITWHGPGQLVGYPIVGLAEPLDVVNFVRRIEEALIAVCT
CCCCCCCCCCCCEEECCCCCEEEECCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
GLGLDAGRVEGRSGVWLPGDGLRPERKIGAIGIRVSRGTTLHGFALNCDCDLSAFSAIVP
CCCCCCCCCCCCCCEEECCCCCCCCCCEEEEEEEEECCCEEEEEEEECCCCHHHHHHHHC
CGIADAGVTSLTAELGRRVTVDEVTDAVAARVCDALDGRLAVSGVSVDTYASGVASTQ
CCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA