Click here to switch to the map view.

The map label for this gene is sucB [H]

Identifier: 119869407

GI number: 119869407

Start: 3538311

End: 3540200

Strand: Direct

Name: sucB [H]

Synonym: Mkms_3375

Alternate gene names: 119869407

Gene position: 3538311-3540200 (Clockwise)

Preceding gene: 119869404

Following gene: 119869408

Centisome position: 61.67

GC content: 67.2

Gene sequence:

>1890_bases
ATGGGTGTGACATTCGACCCGATACGACTGAGCCGTTCGAGGGAGTCACAACACATGGCCGTCTCTGTTCAGATGCCCGC
ACTCGGTGAGAGCGTCACCGAAGGCACCGTCACCCGGTGGCTCAAGCAAGAGGGTGACACGGTCGAACAGGACGAACCCC
TACTCGAAGTGTCGACCGACAAGGTCGACACCGAGATCCCGTCCCCGGCCTCCGGCGTGCTGCAGAAGATCGTGGCGCAG
GAAGACGACACCGTCGAGGTCGGCGGGGAACTCGCCGTGATCGGCGAGGGCGGCGAGGATTCGGGCGACTCCTCCGACGA
CTCGTCTTCCGACGAGGACGAGGACGAGGAGCCTGCGGAGGAAGCGGAGTCGGAGACCACCTCCGAGGAGTCCGACAGCT
CCGAGGAGACCGACAGCTCCGAAGAGACCGAGAGCGAGCCGGAACCCGAACCGGAGCCGAAGAAGGAGTCGAAGCCGAAG
CCGAAGTCCTCGGGTTCGGCGACCCCGGTGACGATGCCCGAGCTGGGTGAGTCCGTCACCGAGGGCACGGTCACCCGGTG
GCTCAAGAAGGTCGGCGACAGCGTCGAGGTCGACGAACCGCTGCTGGAGGTGTCGACCGACAAGGTGGACACCGAGATCC
CCTCGCCGGTCGCGGGCACCCTGCTCGAGATCATCGCCGAGGAGGACGACACGGTCGAGGTCGGCGGGGAACTCGCGAAG
ATCGGCGATGCCGATCAGGCCGAGGCCGAAGAGCCCGAACCGGAGCCGGAACCCGAGCCGGAGCCGGAGCCCGAACCCGA
ACCGGAACCCGAGCCGAAGCAGGAGTCCAAGCCGGAGCCGAAGCCCGAGCCCAAACAGGAGTCCAAGCAGGAAGCCAAGC
CCGAGCCCAAGAAGGAGCCCGAGCCGCAGCAGGATGCCGAGCCGTCCGACGGCAGCGGCCCGTACGTCACGCCGCTGGTG
CGCAAACTGGCCGCCGAGCACGACGTGGACCTGGCCGCCGTCAAGGGCACCGGAGTCGGCGGACGCATCCGCAAGCAGGA
CGTCCTGGCGGCGGCCGAGGCCGACAAGAAGGCCAAGTCCGGCCCCGAGGACGCCGCCGAGGCACCCGGGAAGGCGCCGG
CCGCGGCGACGGCATCCGCGAACGCCTCGGACGCACCGCTGGCTCACCTGCGCGGCACCAAGCAGAAGGCCAACCGGATC
CGTCAGCTGACCGCGAAGAAGACGCGCGAATCCCTGCAGGCGACAGCGCAGTTGACGCAGACCCACGAGGTCGACATGAC
CAAGATCGTGGCGTTGCGGGCCAAGGCGAAAAACGACTTCGCCGAGCGCGAGGGCGTCAACCTGACCTATCTGCCATTCA
TCGCCAGGGCGGTCATCGACGCGCTCAAACAGCATCCGAACGTCAACGCCAGCTACAACGAGGACACCAAGGAGATCACC
TACTACGACGCCGAGCACCTCGGCTTCGCGGTGGACACCGATCAGGGTCTGCTCTCGCCGGTTATCAAGAACGCCGGTGA
CCTCTCGCTGGCCGGGCTGGCCCGCGCGATCGCCGACATCGCCGCGCGTGCCCGCTCCGGTGACCTCAAACCGGACGAAC
TGTCCGGCGGCACCTTCACGATCACCAACATCGGCAGCCAGGGCGCCCTGTTCGACACGCCGATCCTGGTGCCGCCGCAG
GCGGCGATGCTGGGCACCGGTGCGATCGTCAAACGGCCGCGGGTGATCGTCGACGAGTTCGGCAACGAGTCGATCGGCGT
GCGGTCGATCTGCTACCTGCCGCTGACCTACGACCACCGACTCATCGACGGTGCCGACGCCGGTCGCTTCCTGACCACCA
TCAAGCGCCGACTCGAAGAGGGTTCGTTCGAGGCAGACCTGGGTCTGTAA

Upstream 100 bases:

>100_bases
AGTCCCACCGTCTCCCTCGGCTCTCGTCAAACCCCTTTCGATAGTGCCAGAGCAATCACTCGCCGAGTCCACGACGAAGA
CGGCGGGGGAACGTGGAAGG

Downstream 100 bases:

>100_bases
CCCGGTGTCCACCGGAGCCGGGGGGCCGGCGACATCAGGCGGAGCTGTGATCGCGATAGCGGGGTCCTCGGGCCTCATCG
GTTCGGCGTTGGTGTCTGCG

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 629; Mature: 628

Protein sequence:

>629_residues
MGVTFDPIRLSRSRESQHMAVSVQMPALGESVTEGTVTRWLKQEGDTVEQDEPLLEVSTDKVDTEIPSPASGVLQKIVAQ
EDDTVEVGGELAVIGEGGEDSGDSSDDSSSDEDEDEEPAEEAESETTSEESDSSEETDSSEETESEPEPEPEPKKESKPK
PKSSGSATPVTMPELGESVTEGTVTRWLKKVGDSVEVDEPLLEVSTDKVDTEIPSPVAGTLLEIIAEEDDTVEVGGELAK
IGDADQAEAEEPEPEPEPEPEPEPEPEPEPEPKQESKPEPKPEPKQESKQEAKPEPKKEPEPQQDAEPSDGSGPYVTPLV
RKLAAEHDVDLAAVKGTGVGGRIRKQDVLAAAEADKKAKSGPEDAAEAPGKAPAAATASANASDAPLAHLRGTKQKANRI
RQLTAKKTRESLQATAQLTQTHEVDMTKIVALRAKAKNDFAEREGVNLTYLPFIARAVIDALKQHPNVNASYNEDTKEIT
YYDAEHLGFAVDTDQGLLSPVIKNAGDLSLAGLARAIADIAARARSGDLKPDELSGGTFTITNIGSQGALFDTPILVPPQ
AAMLGTGAIVKRPRVIVDEFGNESIGVRSICYLPLTYDHRLIDGADAGRFLTTIKRRLEEGSFEADLGL

Sequences:

>Translated_629_residues
MGVTFDPIRLSRSRESQHMAVSVQMPALGESVTEGTVTRWLKQEGDTVEQDEPLLEVSTDKVDTEIPSPASGVLQKIVAQ
EDDTVEVGGELAVIGEGGEDSGDSSDDSSSDEDEDEEPAEEAESETTSEESDSSEETDSSEETESEPEPEPEPKKESKPK
PKSSGSATPVTMPELGESVTEGTVTRWLKKVGDSVEVDEPLLEVSTDKVDTEIPSPVAGTLLEIIAEEDDTVEVGGELAK
IGDADQAEAEEPEPEPEPEPEPEPEPEPEPEPKQESKPEPKPEPKQESKQEAKPEPKKEPEPQQDAEPSDGSGPYVTPLV
RKLAAEHDVDLAAVKGTGVGGRIRKQDVLAAAEADKKAKSGPEDAAEAPGKAPAAATASANASDAPLAHLRGTKQKANRI
RQLTAKKTRESLQATAQLTQTHEVDMTKIVALRAKAKNDFAEREGVNLTYLPFIARAVIDALKQHPNVNASYNEDTKEIT
YYDAEHLGFAVDTDQGLLSPVIKNAGDLSLAGLARAIADIAARARSGDLKPDELSGGTFTITNIGSQGALFDTPILVPPQ
AAMLGTGAIVKRPRVIVDEFGNESIGVRSICYLPLTYDHRLIDGADAGRFLTTIKRRLEEGSFEADLGL
>Mature_628_residues
GVTFDPIRLSRSRESQHMAVSVQMPALGESVTEGTVTRWLKQEGDTVEQDEPLLEVSTDKVDTEIPSPASGVLQKIVAQE
DDTVEVGGELAVIGEGGEDSGDSSDDSSSDEDEDEEPAEEAESETTSEESDSSEETDSSEETESEPEPEPEPKKESKPKP
KSSGSATPVTMPELGESVTEGTVTRWLKKVGDSVEVDEPLLEVSTDKVDTEIPSPVAGTLLEIIAEEDDTVEVGGELAKI
GDADQAEAEEPEPEPEPEPEPEPEPEPEPEPKQESKPEPKPEPKQESKQEAKPEPKKEPEPQQDAEPSDGSGPYVTPLVR
KLAAEHDVDLAAVKGTGVGGRIRKQDVLAAAEADKKAKSGPEDAAEAPGKAPAAATASANASDAPLAHLRGTKQKANRIR
QLTAKKTRESLQATAQLTQTHEVDMTKIVALRAKAKNDFAEREGVNLTYLPFIARAVIDALKQHPNVNASYNEDTKEITY
YDAEHLGFAVDTDQGLLSPVIKNAGDLSLAGLARAIADIAARARSGDLKPDELSGGTFTITNIGSQGALFDTPILVPPQA
AMLGTGAIVKRPRVIVDEFGNESIGVRSICYLPLTYDHRLIDGADAGRFLTTIKRRLEEGSFEADLGL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=227, Percent_Identity=39.2070484581498, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI110671329, Length=477, Percent_Identity=28.3018867924528, Blast_Score=163, Evalue=5e-40,
Organism=Homo sapiens, GI31711992, Length=600, Percent_Identity=26.3333333333333, Blast_Score=160, Evalue=4e-39,
Organism=Homo sapiens, GI203098816, Length=477, Percent_Identity=26.6247379454927, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI203098753, Length=477, Percent_Identity=26.6247379454927, Blast_Score=136, Evalue=6e-32,
Organism=Homo sapiens, GI260898739, Length=164, Percent_Identity=33.5365853658537, Blast_Score=94, Evalue=5e-19,
Organism=Escherichia coli, GI1786946, Length=455, Percent_Identity=34.0659340659341, Blast_Score=227, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI25146366, Length=229, Percent_Identity=37.9912663755458, Blast_Score=155, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17537937, Length=455, Percent_Identity=28.1318681318681, Blast_Score=145, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI17560088, Length=466, Percent_Identity=30.2575107296137, Blast_Score=142, Evalue=5e-34,
Organism=Caenorhabditis elegans, GI17538894, Length=310, Percent_Identity=27.741935483871, Blast_Score=86, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6320352, Length=454, Percent_Identity=31.7180616740088, Blast_Score=203, Evalue=6e-53,
Organism=Saccharomyces cerevisiae, GI6324258, Length=469, Percent_Identity=27.2921108742004, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI18859875, Length=456, Percent_Identity=29.1666666666667, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24645909, Length=227, Percent_Identity=36.1233480176211, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24582497, Length=311, Percent_Identity=27.0096463022508, Blast_Score=101, Evalue=2e-21,
Organism=Drosophila melanogaster, GI20129315, Length=453, Percent_Identity=25.3863134657837, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR014276
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 67188; Mature: 67057

Theoretical pI: Translated: 4.10; Mature: 4.10

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVTFDPIRLSRSRESQHMAVSVQMPALGESVTEGTVTRWLKQEGDTVEQDEPLLEVSTD
CCCCCCHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEECCC
KVDTEIPSPASGVLQKIVAQEDDTVEVGGELAVIGEGGEDSGDSSDDSSSDEDEDEEPAE
CCCCCCCCCHHHHHHHHHHCCCCCEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHH
EAESETTSEESDSSEETDSSEETESEPEPEPEPKKESKPKPKSSGSATPVTMPELGESVT
HHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
EGTVTRWLKKVGDSVEVDEPLLEVSTDKVDTEIPSPVAGTLLEIIAEEDDTVEVGGELAK
HHHHHHHHHHHCCCCCCCCHHHHHCCCHHCCCCCCHHHHHHHHHHHCCCCCCHHCCHHHH
IGDADQAEAEEPEPEPEPEPEPEPEPEPEPEPKQESKPEPKPEPKQESKQEAKPEPKKEP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCC
EPQQDAEPSDGSGPYVTPLVRKLAAEHDVDLAAVKGTGVGGRIRKQDVLAAAEADKKAKS
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCC
GPEDAAEAPGKAPAAATASANASDAPLAHLRGTKQKANRIRQLTAKKTRESLQATAQLTQ
CCCHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
THEVDMTKIVALRAKAKNDFAEREGVNLTYLPFIARAVIDALKQHPNVNASYNEDTKEIT
HHHCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
YYDAEHLGFAVDTDQGLLSPVIKNAGDLSLAGLARAIADIAARARSGDLKPDELSGGTFT
EECHHHCCEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
ITNIGSQGALFDTPILVPPQAAMLGTGAIVKRPRVIVDEFGNESIGVRSICYLPLTYDHR
EEECCCCCCEECCCCCCCCCHHHHCCCHHHHCCCHHHHHCCCCCCCCEEEEEEEECCCCE
LIDGADAGRFLTTIKRRLEEGSFEADLGL
EECCCCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
GVTFDPIRLSRSRESQHMAVSVQMPALGESVTEGTVTRWLKQEGDTVEQDEPLLEVSTD
CCCCCHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEECCC
KVDTEIPSPASGVLQKIVAQEDDTVEVGGELAVIGEGGEDSGDSSDDSSSDEDEDEEPAE
CCCCCCCCCHHHHHHHHHHCCCCCEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCHHH
EAESETTSEESDSSEETDSSEETESEPEPEPEPKKESKPKPKSSGSATPVTMPELGESVT
HHHHHCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
EGTVTRWLKKVGDSVEVDEPLLEVSTDKVDTEIPSPVAGTLLEIIAEEDDTVEVGGELAK
HHHHHHHHHHHCCCCCCCCHHHHHCCCHHCCCCCCHHHHHHHHHHHCCCCCCHHCCHHHH
IGDADQAEAEEPEPEPEPEPEPEPEPEPEPEPKQESKPEPKPEPKQESKQEAKPEPKKEP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCC
EPQQDAEPSDGSGPYVTPLVRKLAAEHDVDLAAVKGTGVGGRIRKQDVLAAAEADKKAKS
CCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCC
GPEDAAEAPGKAPAAATASANASDAPLAHLRGTKQKANRIRQLTAKKTRESLQATAQLTQ
CCCHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
THEVDMTKIVALRAKAKNDFAEREGVNLTYLPFIARAVIDALKQHPNVNASYNEDTKEIT
HHHCCHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
YYDAEHLGFAVDTDQGLLSPVIKNAGDLSLAGLARAIADIAARARSGDLKPDELSGGTFT
EECHHHCCEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
ITNIGSQGALFDTPILVPPQAAMLGTGAIVKRPRVIVDEFGNESIGVRSICYLPLTYDHR
EEECCCCCCEECCCCCCCCCHHHHCCCHHHHCCCHHHHHCCCCCCCCEEEEEEEECCCCE
LIDGADAGRFLTTIKRRLEEGSFEADLGL
EECCCCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]