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The map label for this gene is cobS [H]

Identifier: 119869400

GI number: 119869400

Start: 3531161

End: 3531907

Strand: Direct

Name: cobS [H]

Synonym: Mkms_3368

Alternate gene names: 119869400

Gene position: 3531161-3531907 (Clockwise)

Preceding gene: 119869399

Following gene: 119869401

Centisome position: 61.55

GC content: 74.56

Gene sequence:

>747_bases
GTGATCGGTTCGCTGGCAGGGGCGTTCGCGTTCGGGACCGTGCTGCCGGTACCTGCGGGCAGCACCGCGACGTTGGGCCG
TGGGGTGATGACCGCGCTGCCCGGAGTCGGGATCGTGCTCGGCGCGGTCGCGGCGGCGGTGCTGTGGGCGGGGTCGTGGG
CGTTCGGACCGCACAGCGCGCTGGCGGGTCTGCTGGCGGTGGCCGTGCTGCTGTTGGCGACCCGCGGGATGCACATCGAC
GGGCTCTCCGACACGGTCGACGGGCTGGGGTGTTACGGCGCGCCCGAACGCGCCCTGCGCGTGATGCGCGACGGGTCGGC
CGGTGCGTTCGGCGCGGCGTCGATCGTGGTCGCGGTGGGCGCGCAGACGTTGGCGTTCTCGATGCTCCCCGGCGGCTGGT
CGGGCGCGGTGGGTGTGGTCGTGGCGGTGACGGCGGGCCGGGTCGCCGCACTGGTGGCGTGCCGGCGCGGCATACCCGCC
GCCGAGGGCAGCGCGCTCGGCGGTCGGGTGGCAGGCACCCAACCCGCGGCGGTGGTGCTGGTGTGGCTGGCAGCCGTTGC
GGCGCTGGCGATTCCGGCCACCGAACGGATCTGGCAGGGTCCGCTGACTGTCGTGGTCGCGGTGGGTATGACCGCGCTGC
TGGTGCGGCACTGCGTGCGCCGGTTCGGCGGGATCACCGGTGACGTGCTGGGCGCCGCGATCGAGGTGACGACGACGGTG
GTCGCGGTGGGGCTGGTGATCAGGTAG

Upstream 100 bases:

>100_bases
TCGGTGAGGGCACCGGCGCCGCGGTCGCCCTGCCCGCGGTGCGCGCCGCGGTGGCCGCACTGGCCGGTATGGCGACCTTC
GACGAGGCCGGCGTCTCGTC

Downstream 100 bases:

>100_bases
CGGCGCTGATCCGCAGTGCGCCCGTTCCCGGCACGGACCCGCCACTCAGGCGGTAGCGTCGGTGACGGTCGGCTGGGCTG
TCCACGGAAGGCAATCATGA

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MIGSLAGAFAFGTVLPVPAGSTATLGRGVMTALPGVGIVLGAVAAAVLWAGSWAFGPHSALAGLLAVAVLLLATRGMHID
GLSDTVDGLGCYGAPERALRVMRDGSAGAFGAASIVVAVGAQTLAFSMLPGGWSGAVGVVVAVTAGRVAALVACRRGIPA
AEGSALGGRVAGTQPAAVVLVWLAAVAALAIPATERIWQGPLTVVVAVGMTALLVRHCVRRFGGITGDVLGAAIEVTTTV
VAVGLVIR

Sequences:

>Translated_248_residues
MIGSLAGAFAFGTVLPVPAGSTATLGRGVMTALPGVGIVLGAVAAAVLWAGSWAFGPHSALAGLLAVAVLLLATRGMHID
GLSDTVDGLGCYGAPERALRVMRDGSAGAFGAASIVVAVGAQTLAFSMLPGGWSGAVGVVVAVTAGRVAALVACRRGIPA
AEGSALGGRVAGTQPAAVVLVWLAAVAALAIPATERIWQGPLTVVVAVGMTALLVRHCVRRFGGITGDVLGAAIEVTTTV
VAVGLVIR
>Mature_248_residues
MIGSLAGAFAFGTVLPVPAGSTATLGRGVMTALPGVGIVLGAVAAAVLWAGSWAFGPHSALAGLLAVAVLLLATRGMHID
GLSDTVDGLGCYGAPERALRVMRDGSAGAFGAASIVVAVGAQTLAFSMLPGGWSGAVGVVVAVTAGRVAALVACRRGIPA
AEGSALGGRVAGTQPAAVVLVWLAAVAALAIPATERIWQGPLTVVVAVGMTALLVRHCVRRFGGITGDVLGAAIEVTTTV
VAVGLVIR

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=247, Percent_Identity=34.0080971659919, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 24231; Mature: 24231

Theoretical pI: Translated: 10.71; Mature: 10.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGSLAGAFAFGTVLPVPAGSTATLGRGVMTALPGVGIVLGAVAAAVLWAGSWAFGPHSA
CCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHH
LAGLLAVAVLLLATRGMHIDGLSDTVDGLGCYGAPERALRVMRDGSAGAFGAASIVVAVG
HHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
AQTLAFSMLPGGWSGAVGVVVAVTAGRVAALVACRRGIPAAEGSALGGRVAGTQPAAVVL
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHH
VWLAAVAALAIPATERIWQGPLTVVVAVGMTALLVRHCVRRFGGITGDVLGAAIEVTTTV
HHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VAVGLVIR
HHHHHHCC
>Mature Secondary Structure
MIGSLAGAFAFGTVLPVPAGSTATLGRGVMTALPGVGIVLGAVAAAVLWAGSWAFGPHSA
CCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHH
LAGLLAVAVLLLATRGMHIDGLSDTVDGLGCYGAPERALRVMRDGSAGAFGAASIVVAVG
HHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
AQTLAFSMLPGGWSGAVGVVVAVTAGRVAALVACRRGIPAAEGSALGGRVAGTQPAAVVL
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHH
VWLAAVAALAIPATERIWQGPLTVVVAVGMTALLVRHCVRRFGGITGDVLGAAIEVTTTV
HHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
VAVGLVIR
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA