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The map label for this gene is pcpB [H]

Identifier: 119868928

GI number: 119868928

Start: 3034617

End: 3036230

Strand: Direct

Name: pcpB [H]

Synonym: Mkms_2896

Alternate gene names: 119868928

Gene position: 3034617-3036230 (Clockwise)

Preceding gene: 119868915

Following gene: 119868929

Centisome position: 52.89

GC content: 69.83

Gene sequence:

>1614_bases
GTGCCCGACACCCTGAACACTGAGGTCCTCATCGCCGGTGCAGGCCCGGTCGGGCTCACCGCGGCGATCGAGCTGACCAG
ACGCGGCGTCGACTGCTTCGTCGTCGACCCCCTGCCGGACCCACCGCAGTACGCCAAAGCCGTTGGCGTACAACCTCGCA
CGCTCGAGGTGTTCGAGAACATGGGTGTGCTGCGGGCGGTCCTCGACGCCGGTATCGAGATGAACGGGCAGTGTGTCTAC
GTCAACGGCGAACCGGTGACGCGACTGGACCTGTCCCTGCCTGCGAACGTCCCGTTCCGGTTCCTCTGCATCCCCCAGTA
CGAGACCGAGCGGATCCTGCGCGAGGAGTTCGCGGCACGTGGCGGGTCGATCCAGCGCGGGTGGGGCCTGACCGGATTCG
CCCAGGACGACGACGGGGTGACCGCGACGGTCTCCACCGACAGCGAGGACCGCACCGTGCGCGCCCGGTACCTCGTCGGC
GCCGACGGTGCGCACAGCGTCGTCCGCAAATCGCTCGGCCTGACCTTCGACGGCGCGGCGTTCGAGGAGCAGTACATGCT
CGGCGACGTCGAGGTGGACTGGACCCAGCCGCGCGGGTACGCCATCCGCTCCATGCACCAGACCGACGGCGTCACCGACG
ATCTGCTGGTGTGCATCCCGCTGCCCGGACGCAACCGGTACCGGATGTCGATGCTGGTACCCGACCACCTGTCGACCCGG
TCGCATGGCGAGGTGGAGCACGGCTTCGAAGCCGGCCGGGCCCCCGAACTACACCACATCCAGGCCGTCCTCGACCGGCT
GTCACCGCAACCCACCACGGCCCGCAACCTGCGGTGGTCGTCGGTCTTCCGGATCAGTCACCGCATCGTCGACTCGTACG
GGTCGGGCCGGGTGTTCGTCGCCGGCGACGCCGCCCACATCCATCCACCGACCGGCGCGCAGGGGATGAACACCGGTATC
CAGGACGCCCACAACCTGGCGTGGAAGCTGGCGCTCGCCGTGTCGGGCAACGCCGCGCCCACCCTGCTCGACACCTACGA
CATCGAACGACGTCCGGTCGGCGAGGAGGTCGTGGGGCGTACGGTGCGCAGCGCCCGACAGGGCATCGGCGCGGACTCCG
ACGACCTCGACCACGTCATGCTCCGGGAGGCTCAGCTGCTCATCGACTACGCCGACAGTCCGATCGTCGCCTCGGCGGGT
CCGGTGTCCGAGCCGCTGCCGGGTGGCCGCGCGCCCGACGCGACCGGACTCAGCCGGGCCGCCGTGTCCGGACCGCTGCG
CCTCTACTCGTTGCTGGGCCGCAACCACACCGCACTGTTCTACGCCGACGGCGACGCCACGGTGACCGACGTCGCCCGGT
TCGAAGCGGCCGCCGAGGCCGCCGCCACCGTCGCGCGCGGCGCTCTCGACGTGTACCTGGTGGCCGCGCCCACCGCCGAG
GTGGACAGCACGGTGCTGCCGTTGCTCCGGGACAACGACGGCGATTTCGCCCGCAGCTACTCCACGGCGGGTACGTCTGT
CTTCGTCATCCGTCCCGACGGGTATCTGGGCTTCGCCGCTCACCGGCTCGACACCGAGGTGTTCGAAGGCTTCCTGCACA
ACACGTTTGGCTGA

Upstream 100 bases:

>100_bases
ACGTCCGAATGGAAGCGCAGGGTCATCGGTTCAGCATGGCGGGCGGGCCGAAGATCATGAAACCCCGCCGCACGGGCTCC
TGCAGGACATACTCATCGCT

Downstream 100 bases:

>100_bases
CGGCCGCCGATGTTCAGACCGTCGATCGACTGGGGTTCGGAACTTGCGAACTCGACTGTGTGGGTGCTCGGATGCTTCCT
GATCACCGCACCGTGCCTTC

Product: pentachlorophenol monooxygenase

Products: NA

Alternate protein names: Pentachlorophenol hydroxylase [H]

Number of amino acids: Translated: 537; Mature: 536

Protein sequence:

>537_residues
MPDTLNTEVLIAGAGPVGLTAAIELTRRGVDCFVVDPLPDPPQYAKAVGVQPRTLEVFENMGVLRAVLDAGIEMNGQCVY
VNGEPVTRLDLSLPANVPFRFLCIPQYETERILREEFAARGGSIQRGWGLTGFAQDDDGVTATVSTDSEDRTVRARYLVG
ADGAHSVVRKSLGLTFDGAAFEEQYMLGDVEVDWTQPRGYAIRSMHQTDGVTDDLLVCIPLPGRNRYRMSMLVPDHLSTR
SHGEVEHGFEAGRAPELHHIQAVLDRLSPQPTTARNLRWSSVFRISHRIVDSYGSGRVFVAGDAAHIHPPTGAQGMNTGI
QDAHNLAWKLALAVSGNAAPTLLDTYDIERRPVGEEVVGRTVRSARQGIGADSDDLDHVMLREAQLLIDYADSPIVASAG
PVSEPLPGGRAPDATGLSRAAVSGPLRLYSLLGRNHTALFYADGDATVTDVARFEAAAEAAATVARGALDVYLVAAPTAE
VDSTVLPLLRDNDGDFARSYSTAGTSVFVIRPDGYLGFAAHRLDTEVFEGFLHNTFG

Sequences:

>Translated_537_residues
MPDTLNTEVLIAGAGPVGLTAAIELTRRGVDCFVVDPLPDPPQYAKAVGVQPRTLEVFENMGVLRAVLDAGIEMNGQCVY
VNGEPVTRLDLSLPANVPFRFLCIPQYETERILREEFAARGGSIQRGWGLTGFAQDDDGVTATVSTDSEDRTVRARYLVG
ADGAHSVVRKSLGLTFDGAAFEEQYMLGDVEVDWTQPRGYAIRSMHQTDGVTDDLLVCIPLPGRNRYRMSMLVPDHLSTR
SHGEVEHGFEAGRAPELHHIQAVLDRLSPQPTTARNLRWSSVFRISHRIVDSYGSGRVFVAGDAAHIHPPTGAQGMNTGI
QDAHNLAWKLALAVSGNAAPTLLDTYDIERRPVGEEVVGRTVRSARQGIGADSDDLDHVMLREAQLLIDYADSPIVASAG
PVSEPLPGGRAPDATGLSRAAVSGPLRLYSLLGRNHTALFYADGDATVTDVARFEAAAEAAATVARGALDVYLVAAPTAE
VDSTVLPLLRDNDGDFARSYSTAGTSVFVIRPDGYLGFAAHRLDTEVFEGFLHNTFG
>Mature_536_residues
PDTLNTEVLIAGAGPVGLTAAIELTRRGVDCFVVDPLPDPPQYAKAVGVQPRTLEVFENMGVLRAVLDAGIEMNGQCVYV
NGEPVTRLDLSLPANVPFRFLCIPQYETERILREEFAARGGSIQRGWGLTGFAQDDDGVTATVSTDSEDRTVRARYLVGA
DGAHSVVRKSLGLTFDGAAFEEQYMLGDVEVDWTQPRGYAIRSMHQTDGVTDDLLVCIPLPGRNRYRMSMLVPDHLSTRS
HGEVEHGFEAGRAPELHHIQAVLDRLSPQPTTARNLRWSSVFRISHRIVDSYGSGRVFVAGDAAHIHPPTGAQGMNTGIQ
DAHNLAWKLALAVSGNAAPTLLDTYDIERRPVGEEVVGRTVRSARQGIGADSDDLDHVMLREAQLLIDYADSPIVASAGP
VSEPLPGGRAPDATGLSRAAVSGPLRLYSLLGRNHTALFYADGDATVTDVARFEAAAEAAATVARGALDVYLVAAPTAEV
DSTVLPLLRDNDGDFARSYSTAGTSVFVIRPDGYLGFAAHRLDTEVFEGFLHNTFG

Specific function: Dechlorination of pentachlorophenol to tetrachlorohydroquinone. Removes also hydrogen and nitro, amino, and cyano groups from benzene ring at the para position in relation to the hydroxyl of phenol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=369, Percent_Identity=30.0813008130081, Blast_Score=134, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: =1.14.13.50 [H]

Molecular weight: Translated: 57768; Mature: 57637

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDTLNTEVLIAGAGPVGLTAAIELTRRGVDCFVVDPLPDPPQYAKAVGVQPRTLEVFEN
CCCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHCCCCCHHHHHHC
MGVLRAVLDAGIEMNGQCVYVNGEPVTRLDLSLPANVPFRFLCIPQYETERILREEFAAR
CHHHHHHHHCCCCCCCEEEEECCCCEEEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHC
GGSIQRGWGLTGFAQDDDGVTATVSTDSEDRTVRARYLVGADGAHSVVRKSLGLTFDGAA
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEEECCCCHHHHHHHHHCCEECCCC
FEEQYMLGDVEVDWTQPRGYAIRSMHQTDGVTDDLLVCIPLPGRNRYRMSMLVPDHLSTR
CCCCEEEECEEEECCCCCCCEEHEEHHCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCC
SHGEVEHGFEAGRAPELHHIQAVLDRLSPQPTTARNLRWSSVFRISHRIVDSYGSGRVFV
CCCCCHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHHHHHCCCCCEEEE
AGDAAHIHPPTGAQGMNTGIQDAHNLAWKLALAVSGNAAPTLLDTYDIERRPVGEEVVGR
ECCCCEECCCCCCCCCCCCHHHHHCEEEEEEEEECCCCCCCEEECCCCCCCCCCHHHHHH
TVRSARQGIGADSDDLDHVMLREAQLLIDYADSPIVASAGPVSEPLPGGRAPDATGLSRA
HHHHHHHCCCCCCCCHHHHHHHHHEEEEEECCCCEEECCCCCCCCCCCCCCCCCCCCHHH
AVSGPLRLYSLLGRNHTALFYADGDATVTDVARFEAAAEAAATVARGALDVYLVAAPTAE
HHCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCC
VDSTVLPLLRDNDGDFARSYSTAGTSVFVIRPDGYLGFAAHRLDTEVFEGFLHNTFG
CCCCEECEEECCCCCHHHHHCCCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PDTLNTEVLIAGAGPVGLTAAIELTRRGVDCFVVDPLPDPPQYAKAVGVQPRTLEVFEN
CCCCCCEEEEEECCCCCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHCCCCCHHHHHHC
MGVLRAVLDAGIEMNGQCVYVNGEPVTRLDLSLPANVPFRFLCIPQYETERILREEFAAR
CHHHHHHHHCCCCCCCEEEEECCCCEEEEEEECCCCCCEEEEEECCHHHHHHHHHHHHHC
GGSIQRGWGLTGFAQDDDGVTATVSTDSEDRTVRARYLVGADGAHSVVRKSLGLTFDGAA
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEEECCCCHHHHHHHHHCCEECCCC
FEEQYMLGDVEVDWTQPRGYAIRSMHQTDGVTDDLLVCIPLPGRNRYRMSMLVPDHLSTR
CCCCEEEECEEEECCCCCCCEEHEEHHCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCC
SHGEVEHGFEAGRAPELHHIQAVLDRLSPQPTTARNLRWSSVFRISHRIVDSYGSGRVFV
CCCCCHHCCCCCCCCCHHHHHHHHHHCCCCCCCHHCCCHHHHHHHHHHHHHCCCCCEEEE
AGDAAHIHPPTGAQGMNTGIQDAHNLAWKLALAVSGNAAPTLLDTYDIERRPVGEEVVGR
ECCCCEECCCCCCCCCCCCHHHHHCEEEEEEEEECCCCCCCEEECCCCCCCCCCHHHHHH
TVRSARQGIGADSDDLDHVMLREAQLLIDYADSPIVASAGPVSEPLPGGRAPDATGLSRA
HHHHHHHCCCCCCCCHHHHHHHHHEEEEEECCCCEEECCCCCCCCCCCCCCCCCCCCHHH
AVSGPLRLYSLLGRNHTALFYADGDATVTDVARFEAAAEAAATVARGALDVYLVAAPTAE
HHCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCC
VDSTVLPLLRDNDGDFARSYSTAGTSVFVIRPDGYLGFAAHRLDTEVFEGFLHNTFG
CCCCEECEEECCCCCHHHHHCCCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7678243; 12169590 [H]