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The map label for this gene is yhjG [H]

Identifier: 119868739

GI number: 119868739

Start: 2829081

End: 2830502

Strand: Direct

Name: yhjG [H]

Synonym: Mkms_2706

Alternate gene names: 119868739

Gene position: 2829081-2830502 (Clockwise)

Preceding gene: 119868738

Following gene: 119868740

Centisome position: 49.31

GC content: 69.55

Gene sequence:

>1422_bases
ATGTTCGATGTGATCATCGCCGGCGGCGGACCGACCGGCCTGATGCTGGCGGCCGAGTTGCGTTTGCACGACGTGGCAGT
GCTGGTGCTGGAAAGGGACGAGGAGCCGGCGGCGCATGTGCGCTCGCTCGGACTGCACGTGCGCAGCATCGAGGTGCTGG
ATCAGCGCGGGATGCTCGAGCGGTTCCTGGCACACGGTGAGCGACACCCATTGGGAGGGTTGTTCGCCGGCATCCCCAAG
CCCGCACTCGAGTTGGACACCGCGCACGGCTACATCCTCGGGATCCGCCAGCCCGTGACCGACCGGCTGTTGGCCGAGCG
TACCGCCGAACTCGGCGCCGAGATCCGGCGCGGCCGTGAGGTGGTCGGGCTGACCCAGGATGCGCACGCCGTGACCGTCG
AGCTGGCCGACGGCGCGCAGCTGCGGGGGCGCTACCTCGTCGGTTGCGACGGCGGGCGCAGTACGGTGCGGAAACTGCTC
GGGGTGGGCTTCACCGGCGACCCCAGCACCACCGAGACGCTGCTCGGCGAGATGATGATGACCGAGGATCCGGCGACCGT
GGCCGCCGTGATCGACGACGTCCGCACCCGGCACTGGCGCTTCGGTGCCATGCCCCTGGGCGACGGAATGCATCGTGTCA
TGGTCCCGGCCGAACGTGTCGCTGAGGACCGCACGGCCCCAACGACATTGGCGGAGTTCCGCGAACGGATGCGGGCGGTG
GCCGGCACCGACTTCGGCGTGCACTCCCCGCGGTGGCTCTCGCGATTCGGAGATGCCACCCGGCTCGCCGATCGCTACCG
CGTCGGTCGGGTGCTGCTGGCCGGCGACGCCGCGCACGTCCACCCGCCCGCCGGTGGACAGGGACTCAACCTCGGAATCC
AGGACGCCTTCAACCTGGGCTGGAAGCTGGCGGCGCAGATCGACGGCTGGGCACCGGAAGGCCTGTTGGACACCTACGAA
ACCGAACGCCGCCCGGTGGCCGCCGACGTGCTGAACACCAGCCGGGCGCAGATGGAACTGATGTCGCTGGAACCGGGTCC
GCGCGCGGTGCGCCGATTGTTGGCGGAACTCTTCGAATTCGACGAGGTGAACCGGTACCTCACCGAGAAGGTCACCGCGA
TCGGTGTCCGCTACGACGTCGGCGCTGGGCATCACCTGCTCGGCCGGCGGCTGCATGACGTCCCGCTGACGCGGGGCCGA
CTGTACGAGTTGATGCGGGATGGCCGCGGGCTGCTGCTCGACCAGACCGGTGAGCTCTCCGTCGGCGGGTGGGCGGCTCG
CGTCGACCACATCGTCGACGTCAGCGAGGAACTGGAGGTGCCGGGCGTGCTCCTGCGGCCCGACGGACACGTCGCGTGGG
TCGGTTCCGATCAGGATGATCTGGTCGCCCATCTGCCCACATGGTTCGGCGCTCCGGACTGA

Upstream 100 bases:

>100_bases
GGCTTGCGGCAAGACCCCCGTCATGCCCCAGAATCGTCGGGCCTCGGCGAAAGGCCTTGTTTCTCCGGACCTTTCGCCAA
TCCGAGAAATGGGGAGATCG

Downstream 100 bases:

>100_bases
TCCGGTCCGCGTCACTACGATTGCCCTCATGTCCGGAGACCTGTTGATCGGTGACGCGCCCTTCTCCCACGACCCCTGGG
TGGCCAGGCGGGAACGCTAC

Product: FAD-binding monooxygenase

Products: H2O; 3-(2,3-dihydroxyphenyl)propionate; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MFDVIIAGGGPTGLMLAAELRLHDVAVLVLERDEEPAAHVRSLGLHVRSIEVLDQRGMLERFLAHGERHPLGGLFAGIPK
PALELDTAHGYILGIRQPVTDRLLAERTAELGAEIRRGREVVGLTQDAHAVTVELADGAQLRGRYLVGCDGGRSTVRKLL
GVGFTGDPSTTETLLGEMMMTEDPATVAAVIDDVRTRHWRFGAMPLGDGMHRVMVPAERVAEDRTAPTTLAEFRERMRAV
AGTDFGVHSPRWLSRFGDATRLADRYRVGRVLLAGDAAHVHPPAGGQGLNLGIQDAFNLGWKLAAQIDGWAPEGLLDTYE
TERRPVAADVLNTSRAQMELMSLEPGPRAVRRLLAELFEFDEVNRYLTEKVTAIGVRYDVGAGHHLLGRRLHDVPLTRGR
LYELMRDGRGLLLDQTGELSVGGWAARVDHIVDVSEELEVPGVLLRPDGHVAWVGSDQDDLVAHLPTWFGAPD

Sequences:

>Translated_473_residues
MFDVIIAGGGPTGLMLAAELRLHDVAVLVLERDEEPAAHVRSLGLHVRSIEVLDQRGMLERFLAHGERHPLGGLFAGIPK
PALELDTAHGYILGIRQPVTDRLLAERTAELGAEIRRGREVVGLTQDAHAVTVELADGAQLRGRYLVGCDGGRSTVRKLL
GVGFTGDPSTTETLLGEMMMTEDPATVAAVIDDVRTRHWRFGAMPLGDGMHRVMVPAERVAEDRTAPTTLAEFRERMRAV
AGTDFGVHSPRWLSRFGDATRLADRYRVGRVLLAGDAAHVHPPAGGQGLNLGIQDAFNLGWKLAAQIDGWAPEGLLDTYE
TERRPVAADVLNTSRAQMELMSLEPGPRAVRRLLAELFEFDEVNRYLTEKVTAIGVRYDVGAGHHLLGRRLHDVPLTRGR
LYELMRDGRGLLLDQTGELSVGGWAARVDHIVDVSEELEVPGVLLRPDGHVAWVGSDQDDLVAHLPTWFGAPD
>Mature_473_residues
MFDVIIAGGGPTGLMLAAELRLHDVAVLVLERDEEPAAHVRSLGLHVRSIEVLDQRGMLERFLAHGERHPLGGLFAGIPK
PALELDTAHGYILGIRQPVTDRLLAERTAELGAEIRRGREVVGLTQDAHAVTVELADGAQLRGRYLVGCDGGRSTVRKLL
GVGFTGDPSTTETLLGEMMMTEDPATVAAVIDDVRTRHWRFGAMPLGDGMHRVMVPAERVAEDRTAPTTLAEFRERMRAV
AGTDFGVHSPRWLSRFGDATRLADRYRVGRVLLAGDAAHVHPPAGGQGLNLGIQDAFNLGWKLAAQIDGWAPEGLLDTYE
TERRPVAADVLNTSRAQMELMSLEPGPRAVRRLLAELFEFDEVNRYLTEKVTAIGVRYDVGAGHHLLGRRLHDVPLTRGR
LYELMRDGRGLLLDQTGELSVGGWAARVDHIVDVSEELEVPGVLLRPDGHVAWVGSDQDDLVAHLPTWFGAPD

Specific function: 3-hydroxyphenylpropionate degradation. [C]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=348, Percent_Identity=31.6091954022989, Blast_Score=129, Evalue=3e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 51682; Mature: 51682

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDVIIAGGGPTGLMLAAELRLHDVAVLVLERDEEPAAHVRSLGLHVRSIEVLDQRGMLE
CEEEEEECCCCCCEEEEEHEEHEEEEEEEEECCCCHHHHHHHHCCHHHHHHHHHHCCHHH
RFLAHGERHPLGGLFAGIPKPALELDTAHGYILGIRQPVTDRLLAERTAELGAEIRRGRE
HHHHCCCCCCCCCHHHCCCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCE
VVGLTQDAHAVTVELADGAQLRGRYLVGCDGGRSTVRKLLGVGFTGDPSTTETLLGEMMM
EEEECCCCEEEEEEECCCCEECCCEEEECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHH
TEDPATVAAVIDDVRTRHWRFGAMPLGDGMHRVMVPAERVAEDRTAPTTLAEFRERMRAV
CCCCHHHHHHHHHHHHHCEEECCCCCCCCCCEEECCHHHHHCCCCCCHHHHHHHHHHHHH
AGTDFGVHSPRWLSRFGDATRLADRYRVGRVLLAGDAAHVHPPAGGQGLNLGIQDAFNLG
HCCCCCCCCCHHHHHCCCHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCCHHHHHCCC
WKLAAQIDGWAPEGLLDTYETERRPVAADVLNTSRAQMELMSLEPGPRAVRRLLAELFEF
EEEEEEECCCCCCCCHHHHHHCCCCHHHHHHCCHHHHEEEECCCCCHHHHHHHHHHHHHH
DEVNRYLTEKVTAIGVRYDVGAGHHLLGRRLHDVPLTRGRLYELMRDGRGLLLDQTGELS
HHHHHHHHHHHHHEEEEEECCCCHHHHHCHHHCCCCCHHHHHHHHHCCCCEEEECCCCCC
VGGWAARVDHIVDVSEELEVPGVLLRPDGHVAWVGSDQDDLVAHLPTWFGAPD
CCHHHHHHHHHHCCHHHCCCCEEEECCCCCEEEECCCCCHHHHHCCCCCCCCC
>Mature Secondary Structure
MFDVIIAGGGPTGLMLAAELRLHDVAVLVLERDEEPAAHVRSLGLHVRSIEVLDQRGMLE
CEEEEEECCCCCCEEEEEHEEHEEEEEEEEECCCCHHHHHHHHCCHHHHHHHHHHCCHHH
RFLAHGERHPLGGLFAGIPKPALELDTAHGYILGIRQPVTDRLLAERTAELGAEIRRGRE
HHHHCCCCCCCCCHHHCCCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCE
VVGLTQDAHAVTVELADGAQLRGRYLVGCDGGRSTVRKLLGVGFTGDPSTTETLLGEMMM
EEEECCCCEEEEEEECCCCEECCCEEEECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHH
TEDPATVAAVIDDVRTRHWRFGAMPLGDGMHRVMVPAERVAEDRTAPTTLAEFRERMRAV
CCCCHHHHHHHHHHHHHCEEECCCCCCCCCCEEECCHHHHHCCCCCCHHHHHHHHHHHHH
AGTDFGVHSPRWLSRFGDATRLADRYRVGRVLLAGDAAHVHPPAGGQGLNLGIQDAFNLG
HCCCCCCCCCHHHHHCCCHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCCCCHHHHHCCC
WKLAAQIDGWAPEGLLDTYETERRPVAADVLNTSRAQMELMSLEPGPRAVRRLLAELFEF
EEEEEEECCCCCCCCHHHHHHCCCCHHHHHHCCHHHHEEEECCCCCHHHHHHHHHHHHHH
DEVNRYLTEKVTAIGVRYDVGAGHHLLGRRLHDVPLTRGRLYELMRDGRGLLLDQTGELS
HHHHHHHHHHHHHEEEEEECCCCHHHHHCHHHCCCCCHHHHHHHHHCCCCEEEECCCCCC
VGGWAARVDHIVDVSEELEVPGVLLRPDGHVAWVGSDQDDLVAHLPTWFGAPD
CCHHHHHHHHHHCCHHHCCCCEEEECCCCCEEEECCCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-(3-hydroxyphenyl)propionate; NADH; O2 [C]

Specific reaction: 3-(3-hydroxyphenyl)propionate + NADH + O2 = H2O + 3-(2,3-dihydroxyphenyl)propionate + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]