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The map label for this gene is 119868625

Identifier: 119868625

GI number: 119868625

Start: 2723084

End: 2724460

Strand: Direct

Name: 119868625

Synonym: Mkms_2590

Alternate gene names: NA

Gene position: 2723084-2724460 (Clockwise)

Preceding gene: 119868624

Following gene: 119868626

Centisome position: 47.46

GC content: 64.85

Gene sequence:

>1377_bases
ATGAAGCGCCTCAACGGGATGGACGCGCTGCTGCTCTACAGCGAAACGCCCAACGTCCACACCCACACGCTCAAGATCGC
GATCCTCAGTCCGGCGGACTCTGATCCTGACGCCGGTTTCGGGGCGTTCCGCCGAAGCCTCGAACGACGGCTGCCACTCC
TGGAGCCTCTGCGCTACAAACTCGTCGAGATACCGGGGCGGCTGCACCATCCGATGTGGCTGGAGAACTGCAGCGTCGAC
CTCGACTACCATCTGCGGCGCGTGAGGGTGCCCGCCCCCGGCGGCAGGCGCGAGCTCGACCGTGTCATCGGCGAAATAGC
CAGTACGCCATTGGATCGGCGATATCCACTGTGGGAGTTCTACTTCGCCGACGGCCTGGCTGACGGCCGATGGGCGCTGA
TCGGCAAGGTGCACCATGCCCTCGCCGACGGCGTGGCCTCAGCCAACCTGCTGGCCCGGCTGATGGACGGCGCAGGTGAT
GTCGCGTCGAGCACCGCCGAACCGTGTGCGCCACCGTCGCCTCGACAACTTCTGCGCGCCGCCGGCCGCGATCATCTGCG
TCAGCTTGCCGAGATGCCCGAAACGGTTCGCGATGTCGCCGCGGGCTCCTGGCGACTGCACCGGCGGGCACGCCAACGCG
GTGAGCAGCCCGACTTCGCGCAGTTGATGCACGCTCCGGCAACGTTTCTCAACCACGTGGTCTCGCCGGCTCGTGCGTTC
GCCAGCGACACGCTACCGCTGGGACTCGTCAAGATCACGGCCAAACACCTGGGGATCACGATCAACGACCTGGTGCTGGC
GATGGCGACTGGCGCTCTGCGCACATTGTCGCTGCGCTACGACGGGGCCGCCGACCGACCCATCGTCGCGTCGGTTCCGC
TGAGCACCGACCAGTCGAGCGAGCGGATCACGGGCAATGAGATCGGTGGTCTCGCCGTATCTCTCCCGGTGCACATCGAC
GACCCGCTGGAGCGTATCCGACTGGTGTCGAAGGCGACCACGATCGCCAAGGAGAACCAACGGCTGTTCGACCAGGAGTT
GTACGGGCGGCTGATGGGTTACGTACCCCCTGCCGTCGCCACCCGAGTTCTACAGTGGGTTGCCGAGCACGACACCGACC
ACCGTCTCATGAACATTCCGATCTCGAACGTTCCAGGACCGCGCAACTATGGTCACTTCGACGGAGCCCGCGTCGATGAG
ATCTGTTCGGTGGGTCCGTTGGCGCCCGGGTGCGGTATGAACATCACCGTGTGGAGCTATGTCGACCAGCTGAACATCTC
GGTGATCACCGACGACGTGACCGTCGGAGACACGTCAGAGGCCACCGACGCTCTCCTCGAGGCATTCCATGAAATTCACT
GCGCTGCCGGCATCTAG

Upstream 100 bases:

>100_bases
AGTACGAGTACGACCCGACGTTCATCATGCGGGGGCTCTCGGCTCTGCACATCGAGTTCGAACCCCTGCCGGACCCCTGA
GGGTGTACGAGAGGTGAGCG

Downstream 100 bases:

>100_bases
CTGTACCGAGACAGGACGTTGGTAGCGGGCGTGTTGGCTTGATGTGAGGAGAACCTCCGGGTGGGGTGTGGCTTGTCTAG
GTCCATACCGTCCGGAGGTT

Product: diacylglycerol O-acyltransferase

Products: NA

Alternate protein names: Putative triacylglycerol synthase Rv0221/MT0231 [H]

Number of amino acids: Translated: 458; Mature: 458

Protein sequence:

>458_residues
MKRLNGMDALLLYSETPNVHTHTLKIAILSPADSDPDAGFGAFRRSLERRLPLLEPLRYKLVEIPGRLHHPMWLENCSVD
LDYHLRRVRVPAPGGRRELDRVIGEIASTPLDRRYPLWEFYFADGLADGRWALIGKVHHALADGVASANLLARLMDGAGD
VASSTAEPCAPPSPRQLLRAAGRDHLRQLAEMPETVRDVAAGSWRLHRRARQRGEQPDFAQLMHAPATFLNHVVSPARAF
ASDTLPLGLVKITAKHLGITINDLVLAMATGALRTLSLRYDGAADRPIVASVPLSTDQSSERITGNEIGGLAVSLPVHID
DPLERIRLVSKATTIAKENQRLFDQELYGRLMGYVPPAVATRVLQWVAEHDTDHRLMNIPISNVPGPRNYGHFDGARVDE
ICSVGPLAPGCGMNITVWSYVDQLNISVITDDVTVGDTSEATDALLEAFHEIHCAAGI

Sequences:

>Translated_458_residues
MKRLNGMDALLLYSETPNVHTHTLKIAILSPADSDPDAGFGAFRRSLERRLPLLEPLRYKLVEIPGRLHHPMWLENCSVD
LDYHLRRVRVPAPGGRRELDRVIGEIASTPLDRRYPLWEFYFADGLADGRWALIGKVHHALADGVASANLLARLMDGAGD
VASSTAEPCAPPSPRQLLRAAGRDHLRQLAEMPETVRDVAAGSWRLHRRARQRGEQPDFAQLMHAPATFLNHVVSPARAF
ASDTLPLGLVKITAKHLGITINDLVLAMATGALRTLSLRYDGAADRPIVASVPLSTDQSSERITGNEIGGLAVSLPVHID
DPLERIRLVSKATTIAKENQRLFDQELYGRLMGYVPPAVATRVLQWVAEHDTDHRLMNIPISNVPGPRNYGHFDGARVDE
ICSVGPLAPGCGMNITVWSYVDQLNISVITDDVTVGDTSEATDALLEAFHEIHCAAGI
>Mature_458_residues
MKRLNGMDALLLYSETPNVHTHTLKIAILSPADSDPDAGFGAFRRSLERRLPLLEPLRYKLVEIPGRLHHPMWLENCSVD
LDYHLRRVRVPAPGGRRELDRVIGEIASTPLDRRYPLWEFYFADGLADGRWALIGKVHHALADGVASANLLARLMDGAGD
VASSTAEPCAPPSPRQLLRAAGRDHLRQLAEMPETVRDVAAGSWRLHRRARQRGEQPDFAQLMHAPATFLNHVVSPARAF
ASDTLPLGLVKITAKHLGITINDLVLAMATGALRTLSLRYDGAADRPIVASVPLSTDQSSERITGNEIGGLAVSLPVHID
DPLERIRLVSKATTIAKENQRLFDQELYGRLMGYVPPAVATRVLQWVAEHDTDHRLMNIPISNVPGPRNYGHFDGARVDE
ICSVGPLAPGCGMNITVWSYVDQLNISVITDDVTVGDTSEATDALLEAFHEIHCAAGI

Specific function: Upon expression in E.coli functions weakly as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has very weak wax synthase activity, incorporating palmityl alcohol into wax esters in the presence of palmit

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014292
- InterPro:   IPR009721
- InterPro:   IPR004255 [H]

Pfam domain/function: PF06974 DUF1298; PF03007 UPF0089 [H]

EC number: =2.3.1.20 [H]

Molecular weight: Translated: 50231; Mature: 50231

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLNGMDALLLYSETPNVHTHTLKIAILSPADSDPDAGFGAFRRSLERRLPLLEPLRYK
CCCCCCCCEEEEEECCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHH
LVEIPGRLHHPMWLENCSVDLDYHLRRVRVPAPGGRRELDRVIGEIASTPLDRRYPLWEF
HHHCCCCCCCCEEHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCEE
YFADGLADGRWALIGKVHHALADGVASANLLARLMDGAGDVASSTAEPCAPPSPRQLLRA
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCHHHHHHH
AGRDHLRQLAEMPETVRDVAAGSWRLHRRARQRGEQPDFAQLMHAPATFLNHVVSPARAF
HCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ASDTLPLGLVKITAKHLGITINDLVLAMATGALRTLSLRYDGAADRPIVASVPLSTDQSS
HCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEEEECCCCCCCEEEECCCCCCCCC
ERITGNEIGGLAVSLPVHIDDPLERIRLVSKATTIAKENQRLFDQELYGRLMGYVPPAVA
CCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
TRVLQWVAEHDTDHRLMNIPISNVPGPRNYGHFDGARVDEICSVGPLAPGCGMNITVWSY
HHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCEEHHHH
VDQLNISVITDDVTVGDTSEATDALLEAFHEIHCAAGI
HHHCEEEEEECCEECCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKRLNGMDALLLYSETPNVHTHTLKIAILSPADSDPDAGFGAFRRSLERRLPLLEPLRYK
CCCCCCCCEEEEEECCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHH
LVEIPGRLHHPMWLENCSVDLDYHLRRVRVPAPGGRRELDRVIGEIASTPLDRRYPLWEF
HHHCCCCCCCCEEHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCEE
YFADGLADGRWALIGKVHHALADGVASANLLARLMDGAGDVASSTAEPCAPPSPRQLLRA
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCHHHHHHH
AGRDHLRQLAEMPETVRDVAAGSWRLHRRARQRGEQPDFAQLMHAPATFLNHVVSPARAF
HCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ASDTLPLGLVKITAKHLGITINDLVLAMATGALRTLSLRYDGAADRPIVASVPLSTDQSS
HCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHEEEEEECCCCCCCEEEECCCCCCCCC
ERITGNEIGGLAVSLPVHIDDPLERIRLVSKATTIAKENQRLFDQELYGRLMGYVPPAVA
CCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
TRVLQWVAEHDTDHRLMNIPISNVPGPRNYGHFDGARVDEICSVGPLAPGCGMNITVWSY
HHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCEEHHHH
VDQLNISVITDDVTVGDTSEATDALLEAFHEIHCAAGI
HHHCEEEEEECCEECCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]