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The map label for this gene is mbtM [H]

Identifier: 119868425

GI number: 119868425

Start: 2506618

End: 2508225

Strand: Reverse

Name: mbtM [H]

Synonym: Mkms_2389

Alternate gene names: 119868425

Gene position: 2508225-2506618 (Counterclockwise)

Preceding gene: 119868426

Following gene: 119868400

Centisome position: 43.72

GC content: 70.4

Gene sequence:

>1608_bases
GTGACGCTGAGGACCAACCCCCTGGCATCGGCGCTGTCCGATTCGATGGCCGCCGCCGACACCGATCTGGTGCTGCTCGA
CCGTGAGAGCGGGGCGTGGCGGCGCCACCCGTGGTCAGAGGTGCTCGCCCGCTCGATGAACGTCGCCGAACGGATCGGCG
ACGCCGACGCGTCCGCGGTGGGTGTGATCGGGGAACCGAACGTCGAGTTCGTCGCCGCGATCGTCGGGTCCTTCCTGGCC
GGGCGAGGCGTGTCCGTGCTACCCGGACCGGTCCGCGGCGCCGCCGCCGACCAGTGGGCGCACAACACCCTGGCCCGTTT
CGCCGGTGCGGGAGTCAGCCACGTCCTGACACACGGCAGCCACCTGGGACACCTGCGCGTCGCCGACGGTTCCGCGGCGG
TCCATGACGTGACGACATGGGCCGGACCGAACCGGTCGGCGCCTCTCGACGCGTCACTGCCCACCGGTGACGTCGCGATC
CTGCAGGGCACCGCCGGTTCGACCGGAACGCCTCGCACGGTGCGACTGTCACCCGATGCCGTACTCGCGAACCTCACCGG
CCTCAGCGCCCGCATCGGTCTCGAGCGGGGCCACGACGTCGGGTGTTCGTGGCTGCCGCTCTACCACGACATGGGGTTGA
GCTTCCTGCTGACCGGGGCGCTCGCCGGAGTCGAGACATGGCAGGCCCCCACATCGGCATTCCAGGCGTCACCCTTCCGG
TGGTTGAACTGGCTCAGCGACAGCCGCGCGACCATCACCGCGGCGCCGAACATGGCGTTCGGGATGATCGGCAAGTACGC
CCGCCGTGTCACCGACGTCGATCTCTCGAACGTCCGGTTCGCGCTCAACGGCGGCGAGCCGGTCGACTGCGAACTCACCG
CGCTGTTCGCCACCGCGATGAGCCGATTCGGATTCTCCGCGGGCGCCCTGGCACCGTCGTACGGACTGGCGGAGTCGACC
TGTGCGGTCACGGTTCCGGAGCCCGGGGACGGTTTGCGCACCGACGAGGTGGCGGTCGGCACGGACGACGGGGTGTCGCT
GCGCCGACACGCGGTCCTGGGTCATGCCATTCCCGGTATGCAGGTGCGCATCTCACCGCGCGAGGGCGACGCCGACGTGT
CCGGGCGCGAGGTCGGCGAGGTCGAGATCCGCGGCTCCTCGATGATGTCGGGTTACGTCGGCGGCGCATCGCTGGATGCG
CAGACCTGGTTCGCCACCGGCGATCTCGGTTATCTCGTCGACGGTGCGCTGGTGGTGTGCGGCCGCGCCAAGGAACTGAT
CACGGTGGCCGGGCGCAACATCTTCCCCGCCGAGATCGAACGGGTGGCCGCCCAGGTGCCGGGGGTACGGGAGGGAGCCG
TCGTCGCGGTGGGCACCGGGGAAGGGTCGATCCGGCCGGGCCTGGTCATCGCGGCGGAGTTCCGTGGTCGCGACGAGGCC
GGTGCGCGAAGCGAATTGGTGCAACAGGTGGCGTCGCAATGCGGTGTGGTGCCCTCGGACGTGGTCTTCGTCAGACCGGG
TTCGCTGCCACGCACGTCGTCGGGCAAGCTGCGCCGCCTCGCGGTCAAGCACAGTCTGGAAGCCGGCACGGAACCGCAGC
CCCGCTGA

Upstream 100 bases:

>100_bases
GACACCCGTCGGCCGGGCTCTGACGTTCTCAGACCGCTTCGAGCGCGGATATGCGGGACTGAGCATAGCCTCACCTATCT
TCGGGTTGTACTGTGCGTGC

Downstream 100 bases:

>100_bases
CGCGTCACTCGCGCGGCAACAGCGGCCCCAGCGGTCCGAGGTCGATGTTGAGGTCCTCGGGTTCGAGACCGAAATGCTCC
CGCAACTCCTCCATCTTCTC

Product: long-chain-fatty-acid--[acyl-carrier-protein] ligase

Products: NA

Alternate protein names: Fatty acyl-[acyl-carrier-protein] synthetase; Fatty acyl-ACP synthetase; Mycobactin synthetase protein M [H]

Number of amino acids: Translated: 535; Mature: 534

Protein sequence:

>535_residues
MTLRTNPLASALSDSMAAADTDLVLLDRESGAWRRHPWSEVLARSMNVAERIGDADASAVGVIGEPNVEFVAAIVGSFLA
GRGVSVLPGPVRGAAADQWAHNTLARFAGAGVSHVLTHGSHLGHLRVADGSAAVHDVTTWAGPNRSAPLDASLPTGDVAI
LQGTAGSTGTPRTVRLSPDAVLANLTGLSARIGLERGHDVGCSWLPLYHDMGLSFLLTGALAGVETWQAPTSAFQASPFR
WLNWLSDSRATITAAPNMAFGMIGKYARRVTDVDLSNVRFALNGGEPVDCELTALFATAMSRFGFSAGALAPSYGLAEST
CAVTVPEPGDGLRTDEVAVGTDDGVSLRRHAVLGHAIPGMQVRISPREGDADVSGREVGEVEIRGSSMMSGYVGGASLDA
QTWFATGDLGYLVDGALVVCGRAKELITVAGRNIFPAEIERVAAQVPGVREGAVVAVGTGEGSIRPGLVIAAEFRGRDEA
GARSELVQQVASQCGVVPSDVVFVRPGSLPRTSSGKLRRLAVKHSLEAGTEPQPR

Sequences:

>Translated_535_residues
MTLRTNPLASALSDSMAAADTDLVLLDRESGAWRRHPWSEVLARSMNVAERIGDADASAVGVIGEPNVEFVAAIVGSFLA
GRGVSVLPGPVRGAAADQWAHNTLARFAGAGVSHVLTHGSHLGHLRVADGSAAVHDVTTWAGPNRSAPLDASLPTGDVAI
LQGTAGSTGTPRTVRLSPDAVLANLTGLSARIGLERGHDVGCSWLPLYHDMGLSFLLTGALAGVETWQAPTSAFQASPFR
WLNWLSDSRATITAAPNMAFGMIGKYARRVTDVDLSNVRFALNGGEPVDCELTALFATAMSRFGFSAGALAPSYGLAEST
CAVTVPEPGDGLRTDEVAVGTDDGVSLRRHAVLGHAIPGMQVRISPREGDADVSGREVGEVEIRGSSMMSGYVGGASLDA
QTWFATGDLGYLVDGALVVCGRAKELITVAGRNIFPAEIERVAAQVPGVREGAVVAVGTGEGSIRPGLVIAAEFRGRDEA
GARSELVQQVASQCGVVPSDVVFVRPGSLPRTSSGKLRRLAVKHSLEAGTEPQPR
>Mature_534_residues
TLRTNPLASALSDSMAAADTDLVLLDRESGAWRRHPWSEVLARSMNVAERIGDADASAVGVIGEPNVEFVAAIVGSFLAG
RGVSVLPGPVRGAAADQWAHNTLARFAGAGVSHVLTHGSHLGHLRVADGSAAVHDVTTWAGPNRSAPLDASLPTGDVAIL
QGTAGSTGTPRTVRLSPDAVLANLTGLSARIGLERGHDVGCSWLPLYHDMGLSFLLTGALAGVETWQAPTSAFQASPFRW
LNWLSDSRATITAAPNMAFGMIGKYARRVTDVDLSNVRFALNGGEPVDCELTALFATAMSRFGFSAGALAPSYGLAESTC
AVTVPEPGDGLRTDEVAVGTDDGVSLRRHAVLGHAIPGMQVRISPREGDADVSGREVGEVEIRGSSMMSGYVGGASLDAQ
TWFATGDLGYLVDGALVVCGRAKELITVAGRNIFPAEIERVAAQVPGVREGAVVAVGTGEGSIRPGLVIAAEFRGRDEAG
ARSELVQQVASQCGVVPSDVVFVRPGSLPRTSSGKLRRLAVKHSLEAGTEPQPR

Specific function: Activates lipidic moieties required for mycobactin biosynthesis. Converts medium- to long-chain aliphatic fatty acids into acyl adenylate, which is further transferred on to the phosphopantetheine arm of the carrier protein MbtL [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=400, Percent_Identity=24.75, Blast_Score=83, Evalue=6e-16,
Organism=Escherichia coli, GI145693145, Length=380, Percent_Identity=25, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17558820, Length=397, Percent_Identity=24.1813602015113, Blast_Score=95, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17560308, Length=468, Percent_Identity=24.7863247863248, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17559526, Length=395, Percent_Identity=25.0632911392405, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71994694, Length=378, Percent_Identity=23.5449735449735, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI71994703, Length=378, Percent_Identity=23.5449735449735, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71994690, Length=378, Percent_Identity=23.5449735449735, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17531443, Length=391, Percent_Identity=24.5524296675192, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI32563687, Length=403, Percent_Identity=22.8287841191067, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17538037, Length=229, Percent_Identity=26.2008733624454, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24581924, Length=406, Percent_Identity=24.8768472906404, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI18859661, Length=392, Percent_Identity=24.234693877551, Blast_Score=83, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21355181, Length=523, Percent_Identity=22.7533460803059, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24653035, Length=215, Percent_Identity=27.4418604651163, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.20 [H]

Molecular weight: Translated: 55542; Mature: 55411

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLRTNPLASALSDSMAAADTDLVLLDRESGAWRRHPWSEVLARSMNVAERIGDADASAV
CCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEE
GVIGEPNVEFVAAIVGSFLAGRGVSVLPGPVRGAAADQWAHNTLARFAGAGVSHVLTHGS
EEECCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCC
HLGHLRVADGSAAVHDVTTWAGPNRSAPLDASLPTGDVAILQGTAGSTGTPRTVRLSPDA
CCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEECCHH
VLANLTGLSARIGLERGHDVGCSWLPLYHDMGLSFLLTGALAGVETWQAPTSAFQASPFR
HHHHCCCCEEEECCCCCCCCCCEEEHHHHHCCHHHHHHHHHHCCHHCCCCHHHHCCCCHH
WLNWLSDSRATITAAPNMAFGMIGKYARRVTDVDLSNVRFALNGGEPVDCELTALFATAM
HHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHH
SRFGFSAGALAPSYGLAESTCAVTVPEPGDGLRTDEVAVGTDDGVSLRRHAVLGHAIPGM
HHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEECCCCCCHHHHHHHHHCCCCCC
QVRISPREGDADVSGREVGEVEIRGSSMMSGYVGGASLDAQTWFATGDLGYLVDGALVVC
EEEECCCCCCCCCCCCCCCEEEECCCCEECCCCCCCCCCCCEEEEECCCHHHHCCEEEEE
GRAKELITVAGRNIFPAEIERVAAQVPGVREGAVVAVGTGEGSIRPGLVIAAEFRGRDEA
CCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCC
GARSELVQQVASQCGVVPSDVVFVRPGSLPRTSSGKLRRLAVKHSLEAGTEPQPR
CHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TLRTNPLASALSDSMAAADTDLVLLDRESGAWRRHPWSEVLARSMNVAERIGDADASAV
CCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEE
GVIGEPNVEFVAAIVGSFLAGRGVSVLPGPVRGAAADQWAHNTLARFAGAGVSHVLTHGS
EEECCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCC
HLGHLRVADGSAAVHDVTTWAGPNRSAPLDASLPTGDVAILQGTAGSTGTPRTVRLSPDA
CCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCEEEEECCHH
VLANLTGLSARIGLERGHDVGCSWLPLYHDMGLSFLLTGALAGVETWQAPTSAFQASPFR
HHHHCCCCEEEECCCCCCCCCCEEEHHHHHCCHHHHHHHHHHCCHHCCCCHHHHCCCCHH
WLNWLSDSRATITAAPNMAFGMIGKYARRVTDVDLSNVRFALNGGEPVDCELTALFATAM
HHHHHCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHH
SRFGFSAGALAPSYGLAESTCAVTVPEPGDGLRTDEVAVGTDDGVSLRRHAVLGHAIPGM
HHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCEEECCCCCCHHHHHHHHHCCCCCC
QVRISPREGDADVSGREVGEVEIRGSSMMSGYVGGASLDAQTWFATGDLGYLVDGALVVC
EEEECCCCCCCCCCCCCCCEEEECCCCEECCCCCCCCCCCCEEEEECCCHHHHCCEEEEE
GRAKELITVAGRNIFPAEIERVAAQVPGVREGAVVAVGTGEGSIRPGLVIAAEFRGRDEA
CCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCCC
GARSELVQQVASQCGVVPSDVVFVRPGSLPRTSSGKLRRLAVKHSLEAGTEPQPR
CHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA