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The map label for this gene is fadA [H]

Identifier: 119868400

GI number: 119868400

Start: 2482336

End: 2483520

Strand: Reverse

Name: fadA [H]

Synonym: Mkms_2364

Alternate gene names: 119868400

Gene position: 2483520-2482336 (Counterclockwise)

Preceding gene: 119868425

Following gene: 119868397

Centisome position: 43.29

GC content: 68.86

Gene sequence:

>1185_bases
ATGAGTGGATTCACGGGACGCGACGCCGTCATCGTCGGGGCGGTACGGACGCCGATCGGCAAGGGTAAGCCGGGCGGCGG
GCTGCACGAGGTGCTGCCGGCCGACCTGCTCGCGCACAGCCTGCGCGAATTGGTCACCCGCACCGGGGTCGATCCCGCCC
AGGTCGACGACGTCATCGCCGGCGCCGTCACCCAGGTGGGCGATCAGGCTGTGAACATCGCCCGCAACGCGCTTCTCGGA
GCGGGCTTTCCGGAGTCGGTTCCCGGCACCACCGTCGACCGCCAGTGCGGTAGCAGCCAGCAGGCCATCAGCTTCGCCGC
TCAGGGCGTGGCCTCCGGCGCGTACGACATCGTGATCGCCGCGGGTGTGGAGTCGATGAGCCGGGTACCGATGGGGTCGT
CGGTCCTGCCCGGCAGCAACCCCTTCGGTGATGACTTCGCCCGCCGCTACCCCGACGGCCTTGTGCCGCAGGGTATTTCC
GCGGAGTTGATCGCCGCCAAGTGGGGGCTCTCACGCACCGCGCTCGACGAGTTCTCCGCGGGCAGCCACGAGAAGGCCGC
GGCGGCGACCAAGGCGGGTCTGTTCGAGGGCGAACTGGCCCCGATCGCCGGACTCGCCACCGACGAGATCATCCGTCCGG
GCACCACCGTGGAGACGCTGGCCGGACTCAAACCGGCGTTCTACCATCCCACCTACGAGGAGCGTTTCCCGCAGATCGGC
TGGGAGATCACCGCGGGCAACTCGTCGCCACTGTCCGACGGCAGCGCCGCGGTGCTGATCACCACGACCGAGGCGGCCAA
ACGGTTGGGGCTGCGGCCGCTGGCGCGTATCCACACCGCGGTGGCCGTCGGATCCGATCCGCTCTACATGCTGACCGGAG
TCATCCCCGCGACCGAGAAGGTGCTGGCCAAGGCGGGCCTGACGCTTGCCGACATCGACCTGTTCGAGGTCAACGAAGCC
TTCGCACCCGTCGTGCTGGCATGGGCACAAGACACCGGCGCCGATCTCGACAAGACGAACGTCAACGGCGGCGCCATCGC
GATCGGTCATCCGTTGGGGGCCAGCGGCGTACGGATCATGACCACGTTGGTCAACGCGTTGGAGCAGCGCGGCGGCCGCT
ACGGGCTGCAGACCATGTGCGAGGCGGGCGGGATGGCCAACGCAACGATCATCGAGCGCCTCTGA

Upstream 100 bases:

>100_bases
AGCACGGCCACGGAATCATCCCCATCTCTGAGTTGTTTTCACTATACCTAGGTGGTACACACGTCTAGGTAGCCACAACC
ATACTCAGAGTGGAGTCGAC

Downstream 100 bases:

>100_bases
GCGATTTCGGCGTGGCTGGTCGCGCTGAGCGCGACCAGCCACGCCGAAATCACGCCGCACTCAGTCGTAGATGACGGCGA
GTCCCTTCCGGCGGAGGTCC

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: Acetyl-CoA acyltransferase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MSGFTGRDAVIVGAVRTPIGKGKPGGGLHEVLPADLLAHSLRELVTRTGVDPAQVDDVIAGAVTQVGDQAVNIARNALLG
AGFPESVPGTTVDRQCGSSQQAISFAAQGVASGAYDIVIAAGVESMSRVPMGSSVLPGSNPFGDDFARRYPDGLVPQGIS
AELIAAKWGLSRTALDEFSAGSHEKAAAATKAGLFEGELAPIAGLATDEIIRPGTTVETLAGLKPAFYHPTYEERFPQIG
WEITAGNSSPLSDGSAAVLITTTEAAKRLGLRPLARIHTAVAVGSDPLYMLTGVIPATEKVLAKAGLTLADIDLFEVNEA
FAPVVLAWAQDTGADLDKTNVNGGAIAIGHPLGASGVRIMTTLVNALEQRGGRYGLQTMCEAGGMANATIIERL

Sequences:

>Translated_394_residues
MSGFTGRDAVIVGAVRTPIGKGKPGGGLHEVLPADLLAHSLRELVTRTGVDPAQVDDVIAGAVTQVGDQAVNIARNALLG
AGFPESVPGTTVDRQCGSSQQAISFAAQGVASGAYDIVIAAGVESMSRVPMGSSVLPGSNPFGDDFARRYPDGLVPQGIS
AELIAAKWGLSRTALDEFSAGSHEKAAAATKAGLFEGELAPIAGLATDEIIRPGTTVETLAGLKPAFYHPTYEERFPQIG
WEITAGNSSPLSDGSAAVLITTTEAAKRLGLRPLARIHTAVAVGSDPLYMLTGVIPATEKVLAKAGLTLADIDLFEVNEA
FAPVVLAWAQDTGADLDKTNVNGGAIAIGHPLGASGVRIMTTLVNALEQRGGRYGLQTMCEAGGMANATIIERL
>Mature_393_residues
SGFTGRDAVIVGAVRTPIGKGKPGGGLHEVLPADLLAHSLRELVTRTGVDPAQVDDVIAGAVTQVGDQAVNIARNALLGA
GFPESVPGTTVDRQCGSSQQAISFAAQGVASGAYDIVIAAGVESMSRVPMGSSVLPGSNPFGDDFARRYPDGLVPQGISA
ELIAAKWGLSRTALDEFSAGSHEKAAAATKAGLFEGELAPIAGLATDEIIRPGTTVETLAGLKPAFYHPTYEERFPQIGW
EITAGNSSPLSDGSAAVLITTTEAAKRLGLRPLARIHTAVAVGSDPLYMLTGVIPATEKVLAKAGLTLADIDLFEVNEAF
APVVLAWAQDTGADLDKTNVNGGAIAIGHPLGASGVRIMTTLVNALEQRGGRYGLQTMCEAGGMANATIIERL

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=404, Percent_Identity=37.6237623762376, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI4501853, Length=400, Percent_Identity=38.5, Blast_Score=231, Evalue=6e-61,
Organism=Homo sapiens, GI148539872, Length=402, Percent_Identity=38.3084577114428, Blast_Score=230, Evalue=2e-60,
Organism=Homo sapiens, GI4557237, Length=404, Percent_Identity=36.1386138613861, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI4504327, Length=433, Percent_Identity=34.8729792147806, Blast_Score=200, Evalue=2e-51,
Organism=Homo sapiens, GI194353979, Length=387, Percent_Identity=29.7157622739018, Blast_Score=142, Evalue=7e-34,
Organism=Escherichia coli, GI48994986, Length=398, Percent_Identity=43.21608040201, Blast_Score=285, Evalue=4e-78,
Organism=Escherichia coli, GI1787663, Length=412, Percent_Identity=40.0485436893204, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI1788554, Length=408, Percent_Identity=40.1960784313725, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI87082165, Length=406, Percent_Identity=38.423645320197, Blast_Score=226, Evalue=1e-60,
Organism=Escherichia coli, GI1788683, Length=435, Percent_Identity=32.4137931034483, Blast_Score=152, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI133906874, Length=409, Percent_Identity=36.6748166259169, Blast_Score=223, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI25147385, Length=404, Percent_Identity=33.4158415841584, Blast_Score=185, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI17551802, Length=437, Percent_Identity=33.6384439359268, Blast_Score=182, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI17535921, Length=409, Percent_Identity=31.2958435207824, Blast_Score=172, Evalue=4e-43,
Organism=Caenorhabditis elegans, GI17535917, Length=412, Percent_Identity=29.3689320388349, Blast_Score=133, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6322031, Length=399, Percent_Identity=37.0927318295739, Blast_Score=203, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6325229, Length=409, Percent_Identity=31.2958435207824, Blast_Score=172, Evalue=9e-44,
Organism=Drosophila melanogaster, GI24655093, Length=405, Percent_Identity=38.7654320987654, Blast_Score=238, Evalue=5e-63,
Organism=Drosophila melanogaster, GI17137578, Length=436, Percent_Identity=34.1743119266055, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI17648125, Length=403, Percent_Identity=34.9875930521092, Blast_Score=194, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24640423, Length=406, Percent_Identity=33.4975369458128, Blast_Score=189, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012805
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 40443; Mature: 40312

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00737 THIOLASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGFTGRDAVIVGAVRTPIGKGKPGGGLHEVLPADLLAHSLRELVTRTGVDPAQVDDVIA
CCCCCCCCEEEEEEHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
GAVTQVGDQAVNIARNALLGAGFPESVPGTTVDRQCGSSQQAISFAAQGVASGAYDIVIA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCEEEEEE
AGVESMSRVPMGSSVLPGSNPFGDDFARRYPDGLVPQGISAELIAAKWGLSRTALDEFSA
CCHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHCCCCHHHHHHHCC
GSHEKAAAATKAGLFEGELAPIAGLATDEIIRPGTTVETLAGLKPAFYHPTYEERFPQIG
CCCHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCHHHHHHCCCCHHCCCCHHHHCCCCC
WEITAGNSSPLSDGSAAVLITTTEAAKRLGLRPLARIHTAVAVGSDPLYMLTGVIPATEK
CEEECCCCCCCCCCCCEEEEEEHHHHHHCCCCHHHHHHHHHHCCCCCCEEECCCCCHHHH
VLAKAGLTLADIDLFEVNEAFAPVVLAWAQDTGADLDKTNVNGGAIAIGHPLGASGVRIM
HHHHCCCEEEECCHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCHHHHH
TTLVNALEQRGGRYGLQTMCEAGGMANATIIERL
HHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHCC
>Mature Secondary Structure 
SGFTGRDAVIVGAVRTPIGKGKPGGGLHEVLPADLLAHSLRELVTRTGVDPAQVDDVIA
CCCCCCCEEEEEEHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
GAVTQVGDQAVNIARNALLGAGFPESVPGTTVDRQCGSSQQAISFAAQGVASGAYDIVIA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHCCCCEEEEEE
AGVESMSRVPMGSSVLPGSNPFGDDFARRYPDGLVPQGISAELIAAKWGLSRTALDEFSA
CCHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCHHHHHHHCCCCHHHHHHHCC
GSHEKAAAATKAGLFEGELAPIAGLATDEIIRPGTTVETLAGLKPAFYHPTYEERFPQIG
CCCHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCHHHHHHCCCCHHCCCCHHHHCCCCC
WEITAGNSSPLSDGSAAVLITTTEAAKRLGLRPLARIHTAVAVGSDPLYMLTGVIPATEK
CEEECCCCCCCCCCCCEEEEEEHHHHHHCCCCHHHHHHHHHHCCCCCCEEECCCCCHHHH
VLAKAGLTLADIDLFEVNEAFAPVVLAWAQDTGADLDKTNVNGGAIAIGHPLGASGVRIM
HHHHCCCEEEECCHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCHHHHH
TTLVNALEQRGGRYGLQTMCEAGGMANATIIERL
HHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA