BacMap

Definition	Mycobacterium sp. KMS chromosome, complete genome.
Accession	NC_008705
Length	5,737,227

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The map label for this gene is braF [H]

Identifier: 119868371

GI number: 119868371

Start: 2447358

End: 2448185

Strand: Reverse

Name: braF [H]

Synonym: Mkms_2335

Alternate gene names: 119868371

Gene position: 2448185-2447358 (Counterclockwise)

Preceding gene: 119868372

Following gene: 119868370

Centisome position: 42.67

GC content: 67.03

Gene sequence:

>828_bases
ATGACGACCACCACCGACCGCGAACCGGTCGCCGGCGGCAACGCCGGCATGGGGGCGGAGTACCTCGAAGTCCGCGGGCT
GACCGTCGATTTCGACGGGTTCAAGGCGGTCAGCGATGTGAACCTGACCCTCTTCCAGGGTGATCTGCGCTTCCTCATCG
GGCCGAACGGGGCCGGTAAGACCACCGTCATCGACGCGATCACCGGCCTGGTCAACGCCTCCGGATCGGTCAACAAGTCC
GGCGTCGAACTGCTCGGCAAGAAGGTGCACCAGATCGCCAAACTCGGCGTCGGACGGACCTTCCAGACCGCGAGCGTCTT
CGAACAGCTCACCGTGTTGCAGAACCTCGACATCGCCGCCGGAGCGCACCGGTCGGCCTGGACGCTGCTGCGCCGCAGGC
ACGGCGTGTCACCGGCCATCGAGGAGGCCCTGGAGACCGTCGGCCTGACCGAGATGGCCGACAAGCCCGCAGGCGTGCTG
GCACACGGTCAGAAGCAGTGGCTCGAGATCGGCATGCTGCTGGTCCAGAACGCCGACGTGCTGCTGCTCGACGAGCCGGT
GGCGGGAATGAGCACCGAGGAACGGGAAGAGACCGGAAACCTGTTGCGCCGCATCGGTGGTCAGCGCACCGTCGTCGTCG
TCGAACACGACATGGATTTCATGCGTGCCTTCGCGACGTCGGTGACGGTGCTGGCCCGCGGCCAGGTGATCGCCGAAGGG
TCGGTCGCCGAGGTGCAGGCGAACCCGAAGGTGCAGGAGGTGTACCTGGGTACCGCCGCCGCGGGTGCCGACGGGATCGC
CGACGACCTCGTCGTGGAAGGTGACTGA

Upstream 100 bases:

>100_bases
TGGCCGGGCTCGGTGTGCTGCTCAAACGACGGCGTAGGAAGAAGTCGGAACCCGTACCCGCACAACCCGATCCGCAACCC
GAGAAGGTGGGGGCGCCCAC

Downstream 100 bases:

>100_bases
CATGCTGCAACTCGTCGACGTCCGCACCGGCTACGGGCGGTCACAGGTCATCCACGGCGCGAGCGTCGAGGTGCCCGCCG
ACGGCGTGGCCGCGGTGATG

Product: ABC transporter-like protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MTTTTDREPVAGGNAGMGAEYLEVRGLTVDFDGFKAVSDVNLTLFQGDLRFLIGPNGAGKTTVIDAITGLVNASGSVNKS
GVELLGKKVHQIAKLGVGRTFQTASVFEQLTVLQNLDIAAGAHRSAWTLLRRRHGVSPAIEEALETVGLTEMADKPAGVL
AHGQKQWLEIGMLLVQNADVLLLDEPVAGMSTEEREETGNLLRRIGGQRTVVVVEHDMDFMRAFATSVTVLARGQVIAEG
SVAEVQANPKVQEVYLGTAAAGADGIADDLVVEGD

Sequences:

>Translated_275_residues
MTTTTDREPVAGGNAGMGAEYLEVRGLTVDFDGFKAVSDVNLTLFQGDLRFLIGPNGAGKTTVIDAITGLVNASGSVNKS
GVELLGKKVHQIAKLGVGRTFQTASVFEQLTVLQNLDIAAGAHRSAWTLLRRRHGVSPAIEEALETVGLTEMADKPAGVL
AHGQKQWLEIGMLLVQNADVLLLDEPVAGMSTEEREETGNLLRRIGGQRTVVVVEHDMDFMRAFATSVTVLARGQVIAEG
SVAEVQANPKVQEVYLGTAAAGADGIADDLVVEGD
>Mature_274_residues
TTTTDREPVAGGNAGMGAEYLEVRGLTVDFDGFKAVSDVNLTLFQGDLRFLIGPNGAGKTTVIDAITGLVNASGSVNKSG
VELLGKKVHQIAKLGVGRTFQTASVFEQLTVLQNLDIAAGAHRSAWTLLRRRHGVSPAIEEALETVGLTEMADKPAGVLA
HGQKQWLEIGMLLVQNADVLLLDEPVAGMSTEEREETGNLLRRIGGQRTVVVVEHDMDFMRAFATSVTVLARGQVIAEGS
VAEVQANPKVQEVYLGTAAAGADGIADDLVVEGD

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4674

COG function: function code R; Uncharacterized ABC-type transport system, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI116734710, Length=207, Percent_Identity=32.3671497584541, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI27881501, Length=219, Percent_Identity=27.8538812785388, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI30795238, Length=219, Percent_Identity=27.8538812785388, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI31657092, Length=212, Percent_Identity=29.2452830188679, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI45446740, Length=253, Percent_Identity=29.2490118577075, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI47078218, Length=253, Percent_Identity=29.2490118577075, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI27262624, Length=243, Percent_Identity=26.3374485596708, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI27262626, Length=243, Percent_Identity=26.3374485596708, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI105990541, Length=213, Percent_Identity=25.8215962441315, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI150417984, Length=236, Percent_Identity=30.0847457627119, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI27436953, Length=246, Percent_Identity=26.4227642276423, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI153792144, Length=234, Percent_Identity=25.2136752136752, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI27477115, Length=230, Percent_Identity=26.9565217391304, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI21536376, Length=215, Percent_Identity=26.9767441860465, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1789864, Length=257, Percent_Identity=36.1867704280156, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI87081791, Length=233, Percent_Identity=29.6137339055794, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1790544, Length=247, Percent_Identity=29.5546558704453, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790525, Length=246, Percent_Identity=28.0487804878049, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI48995001, Length=239, Percent_Identity=28.8702928870293, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1788210, Length=222, Percent_Identity=27.4774774774775, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1789672, Length=226, Percent_Identity=29.646017699115, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1789593, Length=238, Percent_Identity=27.7310924369748, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1790467, Length=222, Percent_Identity=29.2792792792793, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87081782, Length=249, Percent_Identity=30.1204819277108, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788761, Length=241, Percent_Identity=28.6307053941909, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787370, Length=235, Percent_Identity=26.3829787234043, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1786803, Length=232, Percent_Identity=25.8620689655172, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788897, Length=247, Percent_Identity=29.9595141700405, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI87081709, Length=257, Percent_Identity=25.2918287937743, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1787029, Length=240, Percent_Identity=25.8333333333333, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790190, Length=239, Percent_Identity=28.0334728033473, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1788450, Length=225, Percent_Identity=28.4444444444444, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1786872, Length=224, Percent_Identity=29.0178571428571, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1788225, Length=242, Percent_Identity=27.6859504132231, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1786398, Length=224, Percent_Identity=29.4642857142857, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1790739, Length=230, Percent_Identity=27.8260869565217, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1787089, Length=226, Percent_Identity=27.4336283185841, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1789991, Length=223, Percent_Identity=25.1121076233184, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788472, Length=236, Percent_Identity=25, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786563, Length=216, Percent_Identity=28.7037037037037, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787712, Length=234, Percent_Identity=26.0683760683761, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI48994943, Length=230, Percent_Identity=27.8260869565217, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786345, Length=240, Percent_Identity=25, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789032, Length=219, Percent_Identity=25.5707762557078, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788506, Length=221, Percent_Identity=26.2443438914027, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1786253, Length=220, Percent_Identity=28.1818181818182, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787792, Length=238, Percent_Identity=25.2100840336134, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786319, Length=242, Percent_Identity=26.0330578512397, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87082268, Length=234, Percent_Identity=26.9230769230769, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI48994883, Length=244, Percent_Identity=25, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87082267, Length=238, Percent_Identity=23.9495798319328, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1787105, Length=183, Percent_Identity=26.2295081967213, Blast_Score=61, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17510237, Length=228, Percent_Identity=27.6315789473684, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17565586, Length=210, Percent_Identity=30.4761904761905, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI71983523, Length=249, Percent_Identity=30.5220883534137, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71983516, Length=249, Percent_Identity=30.5220883534137, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71983510, Length=249, Percent_Identity=30.5220883534137, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI115533608, Length=202, Percent_Identity=29.7029702970297, Blast_Score=87, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24666092, Length=224, Percent_Identity=25.4464285714286, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI28573571, Length=244, Percent_Identity=26.6393442622951, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28574150, Length=215, Percent_Identity=26.9767441860465, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI116007328, Length=215, Percent_Identity=26.9767441860465, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI221512771, Length=225, Percent_Identity=26.2222222222222, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI161076682, Length=220, Percent_Identity=30.9090909090909, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI161076684, Length=220, Percent_Identity=30.9090909090909, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24581617, Length=220, Percent_Identity=30.9090909090909, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24581615, Length=220, Percent_Identity=30.9090909090909, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24643674, Length=223, Percent_Identity=24.2152466367713, Blast_Score=74, Evalue=9e-14,
Organism=Drosophila melanogaster, GI116007184, Length=218, Percent_Identity=26.605504587156, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI221500365, Length=218, Percent_Identity=26.605504587156, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28573573, Length=216, Percent_Identity=26.8518518518519, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI85724864, Length=204, Percent_Identity=28.4313725490196, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24580555, Length=204, Percent_Identity=28.4313725490196, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI85816269, Length=204, Percent_Identity=28.4313725490196, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24580553, Length=204, Percent_Identity=28.4313725490196, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24581191, Length=203, Percent_Identity=30.0492610837438, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24581189, Length=203, Percent_Identity=30.0492610837438, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24581193, Length=203, Percent_Identity=30.0492610837438, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24581195, Length=203, Percent_Identity=30.0492610837438, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24648314, Length=231, Percent_Identity=21.6450216450216, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 29018; Mature: 28887

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTTDREPVAGGNAGMGAEYLEVRGLTVDFDGFKAVSDVNLTLFQGDLRFLIGPNGAGK
CCCCCCCCCCCCCCCCCCCCEEEEECCEEECCCCEEECCCEEEEEECCEEEEECCCCCCC
TTVIDAITGLVNASGSVNKSGVELLGKKVHQIAKLGVGRTFQTASVFEQLTVLQNLDIAA
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHC
GAHRSAWTLLRRRHGVSPAIEEALETVGLTEMADKPAGVLAHGQKQWLEIGMLLVQNADV
CCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHCCCCCCHHHCCHHHHHHHHHHHCCCCCE
LLLDEPVAGMSTEEREETGNLLRRIGGQRTVVVVEHDMDFMRAFATSVTVLARGQVIAEG
EEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHEECCEEEECC
SVAEVQANPKVQEVYLGTAAAGADGIADDLVVEGD
CEEEEECCCCEEEEEEEHHHCCCCCCCCCEEEECC
>Mature Secondary Structure 
TTTTDREPVAGGNAGMGAEYLEVRGLTVDFDGFKAVSDVNLTLFQGDLRFLIGPNGAGK
CCCCCCCCCCCCCCCCCCCEEEEECCEEECCCCEEECCCEEEEEECCEEEEECCCCCCC
TTVIDAITGLVNASGSVNKSGVELLGKKVHQIAKLGVGRTFQTASVFEQLTVLQNLDIAA
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHC
GAHRSAWTLLRRRHGVSPAIEEALETVGLTEMADKPAGVLAHGQKQWLEIGMLLVQNADV
CCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHCCCCCCHHHCCHHHHHHHHHHHCCCCCE
LLLDEPVAGMSTEEREETGNLLRRIGGQRTVVVVEHDMDFMRAFATSVTVLARGQVIAEG
EEECCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHEECCEEEECC
SVAEVQANPKVQEVYLGTAAAGADGIADDLVVEGD
CEEEEECCCCEEEEEEEHHHCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]