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The map label for this gene is mbtM [H]

Identifier: 119867809

GI number: 119867809

Start: 1879347

End: 1880921

Strand: Reverse

Name: mbtM [H]

Synonym: Mkms_1770

Alternate gene names: 119867809

Gene position: 1880921-1879347 (Counterclockwise)

Preceding gene: 119867810

Following gene: 119867808

Centisome position: 32.78

GC content: 70.16

Gene sequence:

>1575_bases
GTGAACCCGTTGGCGTCGGCGCTGGCCGATGCGATGACGTCCAGCGGCCGCGACCTGGTCGTCCTCGACCACGGGGGCTG
GCGCCGTCATCCCTGGGCGGAAGTGCACCAGCGCGCCGAGAACATCGCCGCCCGCATCACCGAGGACGCCGCCACCGTCG
TCGTCCTCGTCGGCGAACCCACCGTCGAGTTCATCGCGGCGATCCCCGGCACGCTGTATGCCGGTGCCGCGCTGTCGATC
CTGCCCGGGCCCGTCCGCGGCGCCGACCTCGGACAGTGGGCGCGCAACACGATGCAGCGGTGCGCGTCGATCGGGGTTCG
CCTGGTCTTCAGCCACGGCGAGCATCTCGACGCGCTGCGCGCCACCCCGGCCTCGATCCCCACGTGTGACGTGACGGAGA
CGGGGCACGCTCGGCGGGACTCGTCGTTCACACCGCCGGATCACGGTGAGTTCGCGGTGCTGCAGGGCACCGCGGGATCG
ACCGGCATCCCGCGCACGGCCCAGTTGTCACCCGAGGCGGTGCTGGCGAATCTGCGCGGCCTTGTCGCGTGCATCGACAT
CACGCCCGCCGACACCGGCTGCTCGTGGCTGCCGCTCTATCACGACATGGGGCTGGCGTTCCTGCTCACCAATGCGCTCG
GCGGGGCCGAACTCTGGCAGGCGCCGACGACCGCGTTCTCGGCGTCACCGTTTAGCTGGCTGCAATGGATGACCGAGAGC
ACGGCCACGCTGACCGCGGCGCCCAATATGGCCTACGGCCTGATCGGCAAGTACGGTAGCCGGCTCACCGACTTCGACCT
CGGCGCTCTGCGGTTCGCACTCAACGGCGGTGAACCCGTCGACTGCCAGGGTTACCGCAGGTTCGCCACCGAGATGGCGC
GTTTCGGCCTCGACCCGTCCGCCCTCGTGCCCGCCTATGGTCTGGCCGAATCCTCCTGTGCGGTGACCGTTCCGATCCTC
GGCTCCGGCCTACAGGTCGACGAGGTCCAGGTGAGTTCGGACGACGGCCGGTTCATCCGCACACATGCCGTCGTCGGTGA
GCCGATCCCCGGCATGCAGGTGCGGATCGCACCCACGGACGGCTCCGGCGAGCAGGTCGACGGCCGGGACTTCGGAGAGA
TCGAGGTCCGCGGGACGTCGCTGATGTCGGGATACCTCGGCGAACCCCCGCTGGAGGCCGGATCATGGTTCCCCACCGGC
GATCTCGGCTATTTCGTCGACAGCGGGCTCGTTGTGTGCGGTCGCGCGAAGGAGCTGATCACCGTCGCCGGGCGCAACAT
CTTCCCGGCCGAGGTGGAACGCGTCGCCGCCCAGGTGCAGGGCGTCCGGGAAGGGGCGGTCGTCGCCGTCGGCACCGACG
AAAGCGCCGTGCGGCCGGGGCTGGTCATCGCCGCCGAGTTCCGCGGGACCGACGAACCGGCCGCCCGCACTGAACTCGTG
CAGCGGGTGGCGTCGGAATGCGGCGTGGTGCCGTCGAACGTGGTGTTCCTCGAACCCGGCGCCCTCCCCCGCACGTCGTC
GGGCAAGCTGCGCCGGCTGGAGGTCAAACGAAGCATGGAGGACGTGAACCGGTGA

Upstream 100 bases:

>100_bases
TCGGCGTCGCGCTGACCGAGGAAGACCTGCTCAGCTGTGACACGGTCGGCGATCTCGAAGCGGCGATCCAGGCGAAGGTG
CCGAGTTCCCCGAGCGGCCA

Downstream 100 bases:

>100_bases
CACTCGCCGCCCACACCACCGAGGACTACGAAGAACTTCTCGGCCGGGTATTCGATGATCAGGTGAAGGCCTGGACGGCC
GAAGCCGAAGCGTCCGAACG

Product: long-chain-fatty-acid--[acyl-carrier-protein] ligase

Products: NA

Alternate protein names: Fatty acyl-[acyl-carrier-protein] synthetase; Fatty acyl-ACP synthetase; Mycobactin synthetase protein M [H]

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MNPLASALADAMTSSGRDLVVLDHGGWRRHPWAEVHQRAENIAARITEDAATVVVLVGEPTVEFIAAIPGTLYAGAALSI
LPGPVRGADLGQWARNTMQRCASIGVRLVFSHGEHLDALRATPASIPTCDVTETGHARRDSSFTPPDHGEFAVLQGTAGS
TGIPRTAQLSPEAVLANLRGLVACIDITPADTGCSWLPLYHDMGLAFLLTNALGGAELWQAPTTAFSASPFSWLQWMTES
TATLTAAPNMAYGLIGKYGSRLTDFDLGALRFALNGGEPVDCQGYRRFATEMARFGLDPSALVPAYGLAESSCAVTVPIL
GSGLQVDEVQVSSDDGRFIRTHAVVGEPIPGMQVRIAPTDGSGEQVDGRDFGEIEVRGTSLMSGYLGEPPLEAGSWFPTG
DLGYFVDSGLVVCGRAKELITVAGRNIFPAEVERVAAQVQGVREGAVVAVGTDESAVRPGLVIAAEFRGTDEPAARTELV
QRVASECGVVPSNVVFLEPGALPRTSSGKLRRLEVKRSMEDVNR

Sequences:

>Translated_524_residues
MNPLASALADAMTSSGRDLVVLDHGGWRRHPWAEVHQRAENIAARITEDAATVVVLVGEPTVEFIAAIPGTLYAGAALSI
LPGPVRGADLGQWARNTMQRCASIGVRLVFSHGEHLDALRATPASIPTCDVTETGHARRDSSFTPPDHGEFAVLQGTAGS
TGIPRTAQLSPEAVLANLRGLVACIDITPADTGCSWLPLYHDMGLAFLLTNALGGAELWQAPTTAFSASPFSWLQWMTES
TATLTAAPNMAYGLIGKYGSRLTDFDLGALRFALNGGEPVDCQGYRRFATEMARFGLDPSALVPAYGLAESSCAVTVPIL
GSGLQVDEVQVSSDDGRFIRTHAVVGEPIPGMQVRIAPTDGSGEQVDGRDFGEIEVRGTSLMSGYLGEPPLEAGSWFPTG
DLGYFVDSGLVVCGRAKELITVAGRNIFPAEVERVAAQVQGVREGAVVAVGTDESAVRPGLVIAAEFRGTDEPAARTELV
QRVASECGVVPSNVVFLEPGALPRTSSGKLRRLEVKRSMEDVNR
>Mature_524_residues
MNPLASALADAMTSSGRDLVVLDHGGWRRHPWAEVHQRAENIAARITEDAATVVVLVGEPTVEFIAAIPGTLYAGAALSI
LPGPVRGADLGQWARNTMQRCASIGVRLVFSHGEHLDALRATPASIPTCDVTETGHARRDSSFTPPDHGEFAVLQGTAGS
TGIPRTAQLSPEAVLANLRGLVACIDITPADTGCSWLPLYHDMGLAFLLTNALGGAELWQAPTTAFSASPFSWLQWMTES
TATLTAAPNMAYGLIGKYGSRLTDFDLGALRFALNGGEPVDCQGYRRFATEMARFGLDPSALVPAYGLAESSCAVTVPIL
GSGLQVDEVQVSSDDGRFIRTHAVVGEPIPGMQVRIAPTDGSGEQVDGRDFGEIEVRGTSLMSGYLGEPPLEAGSWFPTG
DLGYFVDSGLVVCGRAKELITVAGRNIFPAEVERVAAQVQGVREGAVVAVGTDESAVRPGLVIAAEFRGTDEPAARTELV
QRVASECGVVPSNVVFLEPGALPRTSSGKLRRLEVKRSMEDVNR

Specific function: Activates lipidic moieties required for mycobactin biosynthesis. Converts medium- to long-chain aliphatic fatty acids into acyl adenylate, which is further transferred on to the phosphopantetheine arm of the carrier protein MbtL [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=385, Percent_Identity=25.7142857142857, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI145693145, Length=387, Percent_Identity=26.6149870801034, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1788107, Length=377, Percent_Identity=25.4641909814324, Blast_Score=82, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17558820, Length=371, Percent_Identity=23.989218328841, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI32563687, Length=386, Percent_Identity=23.8341968911917, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17559526, Length=395, Percent_Identity=25.5696202531646, Blast_Score=86, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17560308, Length=380, Percent_Identity=26.5789473684211, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI71994694, Length=425, Percent_Identity=24.7058823529412, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71994703, Length=425, Percent_Identity=24.7058823529412, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71994690, Length=425, Percent_Identity=24.7058823529412, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17531443, Length=400, Percent_Identity=23, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI18859661, Length=414, Percent_Identity=23.6714975845411, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI21355181, Length=547, Percent_Identity=23.400365630713, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21356947, Length=150, Percent_Identity=32, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI20130357, Length=370, Percent_Identity=25.4054054054054, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24581924, Length=377, Percent_Identity=25.7294429708223, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24653035, Length=178, Percent_Identity=29.2134831460674, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.20 [H]

Molecular weight: Translated: 55345; Mature: 55345

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPLASALADAMTSSGRDLVVLDHGGWRRHPWAEVHQRAENIAARITEDAATVVVLVGEP
CCHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHEECCCEEEEEEECCC
TVEFIAAIPGTLYAGAALSILPGPVRGADLGQWARNTMQRCASIGVRLVFSHGEHLDALR
HHHHHHHCCCHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCHHHHH
ATPASIPTCDVTETGHARRDSSFTPPDHGEFAVLQGTAGSTGIPRTAQLSPEAVLANLRG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHCC
LVACIDITPADTGCSWLPLYHDMGLAFLLTNALGGAELWQAPTTAFSASPFSWLQWMTES
EEEEEEECCCCCCCCEEHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHCC
TATLTAAPNMAYGLIGKYGSRLTDFDLGALRFALNGGEPVDCQGYRRFATEMARFGLDPS
CEEEEECCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCH
ALVPAYGLAESSCAVTVPILGSGLQVDEVQVSSDDGRFIRTHAVVGEPIPGMQVRIAPTD
HHCCCCCCCCCCCEEEEEECCCCCEEEEEEEECCCCCEEEEEEEECCCCCCCEEEEEECC
GSGEQVDGRDFGEIEVRGTSLMSGYLGEPPLEAGSWFPTGDLGYFVDSGLVVCGRAKELI
CCCCCCCCCCCCEEEEECHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCEEEECCCCEEH
TVAGRNIFPAEVERVAAQVQGVREGAVVAVGTDESAVRPGLVIAAEFRGTDEPAARTELV
EECCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHH
QRVASECGVVPSNVVFLEPGALPRTSSGKLRRLEVKRSMEDVNR
HHHHHHCCCCCCCEEEECCCCCCCCCCCCEEEHHHHHHHHHHCC
>Mature Secondary Structure
MNPLASALADAMTSSGRDLVVLDHGGWRRHPWAEVHQRAENIAARITEDAATVVVLVGEP
CCHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHEECCCEEEEEEECCC
TVEFIAAIPGTLYAGAALSILPGPVRGADLGQWARNTMQRCASIGVRLVFSHGEHLDALR
HHHHHHHCCCHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCHHHHH
ATPASIPTCDVTETGHARRDSSFTPPDHGEFAVLQGTAGSTGIPRTAQLSPEAVLANLRG
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHCC
LVACIDITPADTGCSWLPLYHDMGLAFLLTNALGGAELWQAPTTAFSASPFSWLQWMTES
EEEEEEECCCCCCCCEEHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHCC
TATLTAAPNMAYGLIGKYGSRLTDFDLGALRFALNGGEPVDCQGYRRFATEMARFGLDPS
CEEEEECCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCH
ALVPAYGLAESSCAVTVPILGSGLQVDEVQVSSDDGRFIRTHAVVGEPIPGMQVRIAPTD
HHCCCCCCCCCCCEEEEEECCCCCEEEEEEEECCCCCEEEEEEEECCCCCCCEEEEEECC
GSGEQVDGRDFGEIEVRGTSLMSGYLGEPPLEAGSWFPTGDLGYFVDSGLVVCGRAKELI
CCCCCCCCCCCCEEEEECHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCEEEECCCCEEH
TVAGRNIFPAEVERVAAQVQGVREGAVVAVGTDESAVRPGLVIAAEFRGTDEPAARTELV
EECCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHH
QRVASECGVVPSNVVFLEPGALPRTSSGKLRRLEVKRSMEDVNR
HHHHHHCCCCCCCEEEECCCCCCCCCCCCEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA