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The map label for this gene is nuoI [H]

Identifier: 119867642

GI number: 119867642

Start: 1713309

End: 1713833

Strand: Reverse

Name: nuoI [H]

Synonym: Mkms_1596

Alternate gene names: 119867642

Gene position: 1713833-1713309 (Counterclockwise)

Preceding gene: 119867643

Following gene: 119867641

Centisome position: 29.87

GC content: 65.9

Gene sequence:

>525_bases
ATGCCTAAGCTGTGGGACGCCGTCGCCGGCTTCGCCGTCACGTTCGGCACGCTGTTCAAGAAACCGATCACCGAGGAGTA
TCCGGAGAAACCCGGTCCGGTCGCTCCCCGGTATCACGGCCGCCATCAACTGAACCGGTACCCCGACGGTCTCGAGAAGT
GCATCGGCTGCGAACTGTGCGCCTGGGCCTGTCCGGCCGACGCGATCTACGTCGAGGGCGACGACAACACCGCCGACGAA
CGCTATTCCCCCGGTGAACGTTACGGCCGCGTCTACCAGATCAACTACCTGCGCTGCATCGGATGCGGGCTGTGCATCGA
GGCCTGTCCGACCAGGGCGCTGACGATGACCAACGACTACGAGATGGCCGACGACAACCGGGCGGATCTGATCTGGGGTA
AGGACAAGCTGCTCGCACCGCTGCAGGACGGGATGCTGGCGCCGCCGCACCCGATGGCGCCCGGCGCCACCGACGACGAT
TACTACCTGGGCCGAATCGGGCCGGCCACCGAGGACGTCCGGTGA

Upstream 100 bases:

>100_bases
CGGACCGTGCCCGCGCGCACCGCCGCGGACGTGTTCCCGATTCCGCCGATACCCGGCCGTGCGGGCACCGCGCGCACGCC
CGAAAGCAGGGAGACGACAG

Downstream 100 bases:

>100_bases
GCCCGGACGCCATGGTGCTCGCCGCGGACGGTGCGGCACGCACCTCCACTAGCGAGGCGGTCGTGTTCTGGATTCTCGGC
GGCATCGCGCTGATCGGGGC

Product: NADH dehydrogenase subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I [H]

Number of amino acids: Translated: 174; Mature: 173

Protein sequence:

>174_residues
MPKLWDAVAGFAVTFGTLFKKPITEEYPEKPGPVAPRYHGRHQLNRYPDGLEKCIGCELCAWACPADAIYVEGDDNTADE
RYSPGERYGRVYQINYLRCIGCGLCIEACPTRALTMTNDYEMADDNRADLIWGKDKLLAPLQDGMLAPPHPMAPGATDDD
YYLGRIGPATEDVR

Sequences:

>Translated_174_residues
MPKLWDAVAGFAVTFGTLFKKPITEEYPEKPGPVAPRYHGRHQLNRYPDGLEKCIGCELCAWACPADAIYVEGDDNTADE
RYSPGERYGRVYQINYLRCIGCGLCIEACPTRALTMTNDYEMADDNRADLIWGKDKLLAPLQDGMLAPPHPMAPGATDDD
YYLGRIGPATEDVR
>Mature_173_residues
PKLWDAVAGFAVTFGTLFKKPITEEYPEKPGPVAPRYHGRHQLNRYPDGLEKCIGCELCAWACPADAIYVEGDDNTADER
YSPGERYGRVYQINYLRCIGCGLCIEACPTRALTMTNDYEMADDNRADLIWGKDKLLAPLQDGMLAPPHPMAPGATDDDY
YLGRIGPATEDVR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be menaquinone. Couples the redox reaction to proton transloca

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI4505371, Length=136, Percent_Identity=38.9705882352941, Blast_Score=110, Evalue=7e-25,
Organism=Escherichia coli, GI1788617, Length=142, Percent_Identity=41.5492957746479, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1789075, Length=127, Percent_Identity=32.2834645669291, Blast_Score=63, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17555194, Length=129, Percent_Identity=41.0852713178295, Blast_Score=108, Evalue=1e-24,
Organism=Drosophila melanogaster, GI17864306, Length=133, Percent_Identity=42.8571428571429, Blast_Score=117, Evalue=3e-27,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 19365; Mature: 19234

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
4.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKLWDAVAGFAVTFGTLFKKPITEEYPEKPGPVAPRYHGRHQLNRYPDGLEKCIGCELC
CCHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCHHHCCCCCHHHHHHCCHHH
AWACPADAIYVEGDDNTADERYSPGERYGRVYQINYLRCIGCGLCIEACPTRALTMTNDY
HCCCCCCEEEEECCCCCCCCCCCCHHHCCEEEEECHHHHHHHHHHHHHCCCCEEEECCCC
EMADDNRADLIWGKDKLLAPLQDGMLAPPHPMAPGATDDDYYLGRIGPATEDVR
CCCCCCCCEEEECCCHHHCHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCC
>Mature Secondary Structure 
PKLWDAVAGFAVTFGTLFKKPITEEYPEKPGPVAPRYHGRHQLNRYPDGLEKCIGCELC
CHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCHHHCCCCCHHHHHHCCHHH
AWACPADAIYVEGDDNTADERYSPGERYGRVYQINYLRCIGCGLCIEACPTRALTMTNDY
HCCCCCCEEEEECCCCCCCCCCCCHHHCCEEEEECHHHHHHHHHHHHHCCCCEEEECCCC
EMADDNRADLIWGKDKLLAPLQDGMLAPPHPMAPGATDDDYYLGRIGPATEDVR
CCCCCCCCEEEECCCHHHCHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA