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The map label for this gene is nuoM [H]

Identifier: 119867635

GI number: 119867635

Start: 1705013

End: 1706569

Strand: Reverse

Name: nuoM [H]

Synonym: Mkms_1589

Alternate gene names: 119867635

Gene position: 1706569-1705013 (Counterclockwise)

Preceding gene: 119867636

Following gene: 119867634

Centisome position: 29.75

GC content: 66.92

Gene sequence:

>1557_bases
GTGGTGATTCCCTGGCTGAGCGTGCTGTGGGCGGCGCCGATCGTCGGCGCCGGTGTGGTGATGTTGGTGCCCCGTCGCAC
TCTGGCGAAGTGGGTGGCGCTGGCGGTGTCGTTGGTCGCGCTGGCGGTCACCGCGGTCGTCGCGGTCGGCTTCGACCCCG
GCGGCGAGCAGTACCAGTTCGTCGAATCCCGCCAATGGATACCGTCGTTCGGCACGGGCTACATCCTCGGTGTCGACGGT
ATCGCCCTGGCGCTGATCGTGTTGACCGCGGTGCTGGTGCCGTTGCTGATCGTCGCCGGGTGGAACGACGCCGACCGCGC
ACCGCAACGCCGGTCCGTGCACACCTACCTCGCGCTGACACTGGCCGTCGAGGGCATGGTGTTCATGTCGCTGGTCGCCC
TCGACGTGCTGCTGTTCTACGTGTTCTTCGAGGCCATGCTGATCCCGATGTACTTCCTCATCGGTGGCTTCGGCGCCGAC
CGGGCGGCATCGTCCAAGGCGGCGGTAAAGTTCCTGCTGTACAACCTGTTCGGCGGTCTGATCATGTTGGCGGCGGTGAT
CGGTCTGTACGTGACCACGGCGGCCAGCGACGCATTCGAGGGGGGCACCTTCGACTTCCGGGCCATCGTCGCGGCCGTCG
CCGCCGGCGACTTCGCGGTGAACCCCGCGGTCCTCAACTTGATGTTCCTCGGTTTCATGTTCGCGTTCGCGGTGAAGGCG
CCGCTGTGGCCGCTGCACCGCTGGTTGCCCGACGCCGCGGTCGAGGCCACCCCCGCGAGCGCGGTGCTGATGATGGCGGT
GATGGACAAGGTCGGCACGTTCGGCATGCTGCGCTACTGCCTGCAGTTGTTCCCCGACGCCGCAACGCTGTTCCGTCCGC
TGATCATCACGCTCGCGGTGGTCGGCATCGTCTACGGGGCGGTGGTCGCCATCGGCCAGACCGACGTGATGCGGTTGATC
GCCTACACGTCGATCTCGCATTTCGGTTTCATCATCCTCGGCATCTTCGTCATGACGAGTCAGGGGCAGTCCGGCTCGGC
GCTGTACATGGTCAACCACGGCATCTCGACCGCGGCACTGTTCCTGATCGCGGGTTTCCTGGTGTCGCAGCGCGGTTCGC
GCCTCATCGCCTCCTACGGCGGCGTGCAGAAGGTGGCGCCGGTACTGGCCGGCACCTTCCTCGTCGCGGGTCTGGCGACG
TTGTCGCTACCCGGGCTGGCCCCGTTCATCAGCGAATTCCTGGTGCTCATCGGCACCTTCACCCGCTACCCGGTGCTGGC
GGTGGTGGCGGCCACCGCGCTGGTGCTCTCCGCGATCTACGTGCTGTGGATGTACCAGCGGATGATGACGGGTCCGGTGC
GCGACGGCAACGAACGGCTGCGTGACCTGGTGCCGCGGGAGCTCGCGGTCGTGACCCCGCTGATCGCGCTTCTGTTGGTG
TTGGGTGTGTATCCGAAACCCGCACTCGACGTGATCGACCCGGCGGTCGGCCACACGCTGACGACCATCAACCAGCCGGA
CCCGACGCCGAAGGTCGCAGAGGGGGCCGCCCGGTGA

Upstream 100 bases:

>100_bases
ACGGATTGCGCGGTCTGCAAACCGGATTCGCCCGGTCCTACGCGCTGGGCATGCTCGGCGGCGCGGCGCTCGTGGTGGCG
GCGATCCTGGCGGTGAACCT

Downstream 100 bases:

>100_bases
TTCCCACTCCTACCGTCGAATACGGTCTGATCTCGCCCATGCTGATCGTGGTGGGCGCCGCGATCGCCGGTGTGCTCGTC
GAGGCGTTCCTGCCCAGGCG

Product: NADH dehydrogenase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MVIPWLSVLWAAPIVGAGVVMLVPRRTLAKWVALAVSLVALAVTAVVAVGFDPGGEQYQFVESRQWIPSFGTGYILGVDG
IALALIVLTAVLVPLLIVAGWNDADRAPQRRSVHTYLALTLAVEGMVFMSLVALDVLLFYVFFEAMLIPMYFLIGGFGAD
RAASSKAAVKFLLYNLFGGLIMLAAVIGLYVTTAASDAFEGGTFDFRAIVAAVAAGDFAVNPAVLNLMFLGFMFAFAVKA
PLWPLHRWLPDAAVEATPASAVLMMAVMDKVGTFGMLRYCLQLFPDAATLFRPLIITLAVVGIVYGAVVAIGQTDVMRLI
AYTSISHFGFIILGIFVMTSQGQSGSALYMVNHGISTAALFLIAGFLVSQRGSRLIASYGGVQKVAPVLAGTFLVAGLAT
LSLPGLAPFISEFLVLIGTFTRYPVLAVVAATALVLSAIYVLWMYQRMMTGPVRDGNERLRDLVPRELAVVTPLIALLLV
LGVYPKPALDVIDPAVGHTLTTINQPDPTPKVAEGAAR

Sequences:

>Translated_518_residues
MVIPWLSVLWAAPIVGAGVVMLVPRRTLAKWVALAVSLVALAVTAVVAVGFDPGGEQYQFVESRQWIPSFGTGYILGVDG
IALALIVLTAVLVPLLIVAGWNDADRAPQRRSVHTYLALTLAVEGMVFMSLVALDVLLFYVFFEAMLIPMYFLIGGFGAD
RAASSKAAVKFLLYNLFGGLIMLAAVIGLYVTTAASDAFEGGTFDFRAIVAAVAAGDFAVNPAVLNLMFLGFMFAFAVKA
PLWPLHRWLPDAAVEATPASAVLMMAVMDKVGTFGMLRYCLQLFPDAATLFRPLIITLAVVGIVYGAVVAIGQTDVMRLI
AYTSISHFGFIILGIFVMTSQGQSGSALYMVNHGISTAALFLIAGFLVSQRGSRLIASYGGVQKVAPVLAGTFLVAGLAT
LSLPGLAPFISEFLVLIGTFTRYPVLAVVAATALVLSAIYVLWMYQRMMTGPVRDGNERLRDLVPRELAVVTPLIALLLV
LGVYPKPALDVIDPAVGHTLTTINQPDPTPKVAEGAAR
>Mature_518_residues
MVIPWLSVLWAAPIVGAGVVMLVPRRTLAKWVALAVSLVALAVTAVVAVGFDPGGEQYQFVESRQWIPSFGTGYILGVDG
IALALIVLTAVLVPLLIVAGWNDADRAPQRRSVHTYLALTLAVEGMVFMSLVALDVLLFYVFFEAMLIPMYFLIGGFGAD
RAASSKAAVKFLLYNLFGGLIMLAAVIGLYVTTAASDAFEGGTFDFRAIVAAVAAGDFAVNPAVLNLMFLGFMFAFAVKA
PLWPLHRWLPDAAVEATPASAVLMMAVMDKVGTFGMLRYCLQLFPDAATLFRPLIITLAVVGIVYGAVVAIGQTDVMRLI
AYTSISHFGFIILGIFVMTSQGQSGSALYMVNHGISTAALFLIAGFLVSQRGSRLIASYGGVQKVAPVLAGTFLVAGLAT
LSLPGLAPFISEFLVLIGTFTRYPVLAVVAATALVLSAIYVLWMYQRMMTGPVRDGNERLRDLVPRELAVVTPLIALLLV
LGVYPKPALDVIDPAVGHTLTTINQPDPTPKVAEGAAR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be menaquinone. Couples the redox reaction to proton transloca

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=389, Percent_Identity=28.7917737789203, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI251831117, Length=265, Percent_Identity=24.5283018867925, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1788613, Length=512, Percent_Identity=33.984375, Blast_Score=261, Evalue=7e-71,
Organism=Escherichia coli, GI1788829, Length=418, Percent_Identity=28.4688995215311, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1788827, Length=411, Percent_Identity=25.7907542579075, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI1788614, Length=467, Percent_Identity=28.6937901498929, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788831, Length=296, Percent_Identity=24.3243243243243, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI145693160, Length=344, Percent_Identity=26.453488372093, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 55076; Mature: 55076

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS00673 V8_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCCCCCCHHHHCHHHCCEEEECCCCCCCCHHCCCCCC
>Mature Secondary Structure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HCCCCCCCHHHHCHHHCCEEEECCCCCCCCHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]