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The map label for this gene is prfC [H]

Identifier: 119867564

GI number: 119867564

Start: 1629579

End: 1631216

Strand: Direct

Name: prfC [H]

Synonym: Mkms_1516

Alternate gene names: 119867564

Gene position: 1629579-1631216 (Clockwise)

Preceding gene: 119867562

Following gene: 119867569

Centisome position: 28.4

GC content: 67.03

Gene sequence:

>1638_bases
ATGACCGATACCGCCTCAGGTGTGTCGACGGCCACCGAGTCGCGGCCAGACCGAGTTTCCGCCGAAGCGCAACGTCGCAG
GACGTTCGCTGTGATCAGCCACCCCGACGCCGGCAAGTCCACCTTGACCGAGGCGCTGGTGCTGCACGCCAAGGCGATCA
CCGAGGCCGGCGCCATCCACGGTAAGGCGGGCCGCCGCGCCACGGTGTCTGACTGGATGGAGATGGAGAAGGCCCGCGGC
ATCTCCATCACCTCGACGGCGCTGCAGTTCCCTTACCGCACCCAGGCGGGCGACACCTGCATCATCAACCTGCTCGACAC
CCCGGGGCACGCCGACTTCTCCGAAGACACCTACCGGGTGCTGACCGCCGTCGATTGCGCCGTGATGCTCATCGACGCCG
CGAAAGGTCTCGAACCGCAGACGCTGAAGCTCTTCCAGGTCTGCCGCCACCGCGGGATCCCCATCATCACGGTGGTCAAC
AAGTGGGACCGGCCGGGCCGTCACGCCCTGGAATTGATGGATGAGATCCACGACCGCATTCAACTGCAGACGACGCCGCT
GACCTGGCCGGTCGGCATCGCCGGCGACTTCAAAGGAGTCCTCGACCGGCGCAGCGGCACCTTCATCCGGTTCACCCGCA
CCGCGGGCGGCGCCACCGCCGCACCGGAGGAGCACATCGATGCGTCCCAGGCCCACGAGGCCGCGGGTACCGACTGGGAC
ACCGCGGTGGAAGAGTCCGAACTGCTGAGTGCCGACGGCGCCGACTTCGACCGCGACTCGTTCCTCAGGTGCACCTCGAC
ACCGGTCCTGTTCACGTCGGCGGTGCTCAACTTCGGTGTCAACCAATTGCTCGACGTGTTGGCCCAACTCGCACCGCCGC
CACGCGGTCAGCTCGACATCGACGGCAGTCGACGCGAAACCACCGCGCCGTTCAGTGCGTTCGTGTTCAAGGTCCAGGCG
GGCATGGACTCCGCTCACCGGGACCGCATCGCCTACGCCCGGGTCGTTTCAGGCACCTTCGAACGCGGAGACGTCCTCAC
CCACGCCACCACCGGCAAGCCGTTCGTCACCAAGTACGCGCAATCGGTCTTCGGCCAGCAGCGGTCCACCCTGGACACGG
CCTGGCCGGGGGATGTCATCGGACTCGCCAACGCCGCCGCGCTGCGACCGGGTGACACCCTGTACCGGGATGTCCCGGTG
CAGTACCCGCCGATTCCGAGCTTCTCGCCCGAGCATTTCGCCGTCGCGCGGGGCACCGACCCCAGCAAGCACAAGCAGTT
CCGTAAAGGGATCGAGCAGCTGGACCAGGAGGGCGTCATCCAGGTGCTGCGTTCGGACCGGCGAGGTGATCAGGCGCCGG
TGTTCGCCGCGGTGGGTCCGATGCAGTTCGAGGTCGCCACTCATCGGATGGCCGGCGAGTTCAACGCGCCGATCGCGCTC
GAGCCGCTTCCGTACACGGTGGCCCGCGTCGCCGACCCCGATGACGTCGAGATCCTGCAGAAGCAGGCCTCGGTCGAGGT
CTTCACCCGCACCGACGGAGTCGTCATCGCGGTGTTCTCGAACAAATGGCGCCTCGAGAACGTCGAGCGGGAACTTCCCG
ACGTACGGCTGCGGTCACTGGTGGCAGCAGCAGACTAG

Upstream 100 bases:

>100_bases
TTCGACTGCTGGTGGGCAGCGCGCCGCGGTGACGGCGAATCCGGCCGACGACGGTACCGATTATCGTTTCGACTCCGTGG
CCAATAGAATTGGCGGCGCA

Downstream 100 bases:

>100_bases
GTCTAACGCATACCGACCGCCGCGTCATGGACGTGTGAACAGGGGCCGAGGCTGGCGTTGGCATCGGTGACCAGGGCCTG
GCGACGTGATTCCTCCACCG

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MTDTASGVSTATESRPDRVSAEAQRRRTFAVISHPDAGKSTLTEALVLHAKAITEAGAIHGKAGRRATVSDWMEMEKARG
ISITSTALQFPYRTQAGDTCIINLLDTPGHADFSEDTYRVLTAVDCAVMLIDAAKGLEPQTLKLFQVCRHRGIPIITVVN
KWDRPGRHALELMDEIHDRIQLQTTPLTWPVGIAGDFKGVLDRRSGTFIRFTRTAGGATAAPEEHIDASQAHEAAGTDWD
TAVEESELLSADGADFDRDSFLRCTSTPVLFTSAVLNFGVNQLLDVLAQLAPPPRGQLDIDGSRRETTAPFSAFVFKVQA
GMDSAHRDRIAYARVVSGTFERGDVLTHATTGKPFVTKYAQSVFGQQRSTLDTAWPGDVIGLANAAALRPGDTLYRDVPV
QYPPIPSFSPEHFAVARGTDPSKHKQFRKGIEQLDQEGVIQVLRSDRRGDQAPVFAAVGPMQFEVATHRMAGEFNAPIAL
EPLPYTVARVADPDDVEILQKQASVEVFTRTDGVVIAVFSNKWRLENVERELPDVRLRSLVAAAD

Sequences:

>Translated_545_residues
MTDTASGVSTATESRPDRVSAEAQRRRTFAVISHPDAGKSTLTEALVLHAKAITEAGAIHGKAGRRATVSDWMEMEKARG
ISITSTALQFPYRTQAGDTCIINLLDTPGHADFSEDTYRVLTAVDCAVMLIDAAKGLEPQTLKLFQVCRHRGIPIITVVN
KWDRPGRHALELMDEIHDRIQLQTTPLTWPVGIAGDFKGVLDRRSGTFIRFTRTAGGATAAPEEHIDASQAHEAAGTDWD
TAVEESELLSADGADFDRDSFLRCTSTPVLFTSAVLNFGVNQLLDVLAQLAPPPRGQLDIDGSRRETTAPFSAFVFKVQA
GMDSAHRDRIAYARVVSGTFERGDVLTHATTGKPFVTKYAQSVFGQQRSTLDTAWPGDVIGLANAAALRPGDTLYRDVPV
QYPPIPSFSPEHFAVARGTDPSKHKQFRKGIEQLDQEGVIQVLRSDRRGDQAPVFAAVGPMQFEVATHRMAGEFNAPIAL
EPLPYTVARVADPDDVEILQKQASVEVFTRTDGVVIAVFSNKWRLENVERELPDVRLRSLVAAAD
>Mature_544_residues
TDTASGVSTATESRPDRVSAEAQRRRTFAVISHPDAGKSTLTEALVLHAKAITEAGAIHGKAGRRATVSDWMEMEKARGI
SITSTALQFPYRTQAGDTCIINLLDTPGHADFSEDTYRVLTAVDCAVMLIDAAKGLEPQTLKLFQVCRHRGIPIITVVNK
WDRPGRHALELMDEIHDRIQLQTTPLTWPVGIAGDFKGVLDRRSGTFIRFTRTAGGATAAPEEHIDASQAHEAAGTDWDT
AVEESELLSADGADFDRDSFLRCTSTPVLFTSAVLNFGVNQLLDVLAQLAPPPRGQLDIDGSRRETTAPFSAFVFKVQAG
MDSAHRDRIAYARVVSGTFERGDVLTHATTGKPFVTKYAQSVFGQQRSTLDTAWPGDVIGLANAAALRPGDTLYRDVPVQ
YPPIPSFSPEHFAVARGTDPSKHKQFRKGIEQLDQEGVIQVLRSDRRGDQAPVFAAVGPMQFEVATHRMAGEFNAPIALE
PLPYTVARVADPDDVEILQKQASVEVFTRTDGVVIAVFSNKWRLENVERELPDVRLRSLVAAAD

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=488, Percent_Identity=24.5901639344262, Blast_Score=141, Evalue=1e-33,
Organism=Homo sapiens, GI25306283, Length=347, Percent_Identity=27.9538904899135, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI19923640, Length=347, Percent_Identity=27.9538904899135, Blast_Score=102, Evalue=8e-22,
Organism=Homo sapiens, GI25306287, Length=298, Percent_Identity=29.1946308724832, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI94966754, Length=268, Percent_Identity=25.3731343283582, Blast_Score=84, Evalue=5e-16,
Organism=Homo sapiens, GI157426893, Length=137, Percent_Identity=32.1167883211679, Blast_Score=83, Evalue=8e-16,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=34.2281879194631, Blast_Score=74, Evalue=4e-13,
Organism=Homo sapiens, GI310132016, Length=114, Percent_Identity=34.2105263157895, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI310110807, Length=114, Percent_Identity=34.2105263157895, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI310123363, Length=114, Percent_Identity=34.2105263157895, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1790835, Length=522, Percent_Identity=39.0804597701149, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1789738, Length=490, Percent_Identity=27.9591836734694, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1788922, Length=159, Percent_Identity=33.3333333333333, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI48994988, Length=183, Percent_Identity=33.3333333333333, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=489, Percent_Identity=23.5173824130879, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17556745, Length=181, Percent_Identity=32.0441988950276, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17557151, Length=155, Percent_Identity=31.6129032258064, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71988819, Length=142, Percent_Identity=31.6901408450704, Blast_Score=72, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI71988811, Length=142, Percent_Identity=31.6901408450704, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17506493, Length=174, Percent_Identity=29.3103448275862, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=487, Percent_Identity=25.2566735112936, Blast_Score=143, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6322359, Length=562, Percent_Identity=24.1992882562278, Blast_Score=121, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6323320, Length=157, Percent_Identity=30.5732484076433, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324707, Length=150, Percent_Identity=36, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320593, Length=150, Percent_Identity=36, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24582462, Length=516, Percent_Identity=25.968992248062, Blast_Score=149, Evalue=7e-36,
Organism=Drosophila melanogaster, GI221458488, Length=389, Percent_Identity=27.5064267352185, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI78706572, Length=141, Percent_Identity=35.4609929078014, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=32.6797385620915, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=32.6797385620915, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=32.6797385620915, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU [H]

EC number: NA

Molecular weight: Translated: 59464; Mature: 59332

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTASGVSTATESRPDRVSAEAQRRRTFAVISHPDAGKSTLTEALVLHAKAITEAGAIH
CCCCCCCCCHHHCCCCCCCCHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCC
GKAGRRATVSDWMEMEKARGISITSTALQFPYRTQAGDTCIINLLDTPGHADFSEDTYRV
CCCCCCCHHHHHHHHHHHCCCEEEHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHH
LTAVDCAVMLIDAAKGLEPQTLKLFQVCRHRGIPIITVVNKWDRPGRHALELMDEIHDRI
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHE
QLQTTPLTWPVGIAGDFKGVLDRRSGTFIRFTRTAGGATAAPEEHIDASQAHEAAGTDWD
EEEECCCEECCCCCCCHHHHHHCCCCCEEEEEECCCCCCCCCHHHCCHHHHHHHCCCCHH
TAVEESELLSADGADFDRDSFLRCTSTPVLFTSAVLNFGVNQLLDVLAQLAPPPRGQLDI
HHHHHHHHHCCCCCCCCCCCCEEECCCCHHHHHHHHHHCHHHHHHHHHHHCCCCCCCEEC
DGSRRETTAPFSAFVFKVQAGMDSAHRDRIAYARVVSGTFERGDVLTHATTGKPFVTKYA
CCCCCCCCCCHHHEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHH
QSVFGQQRSTLDTAWPGDVIGLANAAALRPGDTLYRDVPVQYPPIPSFSPEHFAVARGTD
HHHHCCHHHCCCCCCCCCHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCEEEECCCC
PSKHKQFRKGIEQLDQEGVIQVLRSDRRGDQAPVFAAVGPMQFEVATHRMAGEFNAPIAL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCEEEEHHHHHHCCCCCCCEEE
EPLPYTVARVADPDDVEILQKQASVEVFTRTDGVVIAVFSNKWRLENVERELPDVRLRSL
CCCCCHHHCCCCCCHHHHHHHHCCEEEEEECCCEEEEEECCCCCHHHHHHHCCHHHHHHH
VAAAD
HHHCC
>Mature Secondary Structure 
TDTASGVSTATESRPDRVSAEAQRRRTFAVISHPDAGKSTLTEALVLHAKAITEAGAIH
CCCCCCCCHHHCCCCCCCCHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCC
GKAGRRATVSDWMEMEKARGISITSTALQFPYRTQAGDTCIINLLDTPGHADFSEDTYRV
CCCCCCCHHHHHHHHHHHCCCEEEHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHH
LTAVDCAVMLIDAAKGLEPQTLKLFQVCRHRGIPIITVVNKWDRPGRHALELMDEIHDRI
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHE
QLQTTPLTWPVGIAGDFKGVLDRRSGTFIRFTRTAGGATAAPEEHIDASQAHEAAGTDWD
EEEECCCEECCCCCCCHHHHHHCCCCCEEEEEECCCCCCCCCHHHCCHHHHHHHCCCCHH
TAVEESELLSADGADFDRDSFLRCTSTPVLFTSAVLNFGVNQLLDVLAQLAPPPRGQLDI
HHHHHHHHHCCCCCCCCCCCCEEECCCCHHHHHHHHHHCHHHHHHHHHHHCCCCCCCEEC
DGSRRETTAPFSAFVFKVQAGMDSAHRDRIAYARVVSGTFERGDVLTHATTGKPFVTKYA
CCCCCCCCCCHHHEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHH
QSVFGQQRSTLDTAWPGDVIGLANAAALRPGDTLYRDVPVQYPPIPSFSPEHFAVARGTD
HHHHCCHHHCCCCCCCCCHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCEEEECCCC
PSKHKQFRKGIEQLDQEGVIQVLRSDRRGDQAPVFAAVGPMQFEVATHRMAGEFNAPIAL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCEEEEHHHHHHCCCCCCCEEE
EPLPYTVARVADPDDVEILQKQASVEVFTRTDGVVIAVFSNKWRLENVERELPDVRLRSL
CCCCCHHHCCCCCCHHHHHHHHCCEEEEEECCCEEEEEECCCCCHHHHHHHCCHHHHHHH
VAAAD
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA