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The map label for this gene is ackA [H]

Identifier: 119899928

GI number: 119899928

Start: 3993942

End: 3995129

Strand: Direct

Name: ackA [H]

Synonym: azo3639

Alternate gene names: 119899928

Gene position: 3993942-3995129 (Clockwise)

Preceding gene: 119899927

Following gene: 119899940

Centisome position: 91.27

GC content: 69.7

Gene sequence:

>1188_bases
ATGAAGGCCGTCCTCGTCCTCAATGCGGGCTCCAGCAGCCTGAAGTTCGCGCTGTTTCCGATCGAGCCGGCGCTCGCCGA
CCACGCCGCGCTGTCCGGCCAGGTCGAAGGCATCGGCGCGACGCCGGAATTCAGCGCCAAGACCGCCGCCGGCGAACGCT
TCCACGAAACGGTGCCGAGCAACTCGGACAGCCAGCGCGAACAGCACCGCGAAGCGCTCACCTTCCTGCTCGACTGGCTG
CAGGCCCACAACCCCGCGCTGGAGATCGCTGCGGCCGGCCACCGCATCGTGCATGGCGGTGACAAGTACAGCGAACCGGT
GCAACTCTACGGCCACATCCTGCGCCAGTTGCAGAAGCTGGTGCCGCTGGCGCCGCTGCACCAGCCGCACAACCTCAACG
CGGTGCAGGCGATCGCCGCGCTCGCGCCGGACATTCCGCAGGTCGGCTGCTTCGACACCGCCTTCCACACCACCCAGGCG
CCGGTCGCGCAGGCCTTCGCGCTGCCGCGCGCGATCAGCGCCGAAGGCGTCAAGCGCTACGGCTTCCACGGCCTGTCCTA
CGACTACGTCGCCCGCCAGCTGCCGGCGGTGATCGGCGACAAGGCGAGCGGCGCGGTGGTGATCTGCCACCTCGGCAATG
GTTCGTCGATGTGCGCGCTGCGCGACGGCAAGAGCGTCGCCTCGACCATGGGCTTTACCGCGCTCGAAGGCCTGGTGATG
GGCACCCGCACCGGCAGCCTGGACCCCGGCGTGCTGCTCTACCTGATGGACGAGAAGGGCATGGACGCCAAGGCGCTGAC
CCGGCTGCTCTACAAGGAATCCGGCCTGCTCGGCGTCTCCGGCATCAGCCAGGACATGCGCACCCTGCTCGCGTCCGACG
CGCCGGAGGCGGCGGAAGCGGTGGATCTGTACTGCTACCGCATCGCCCGCGAGCTTGGATCGCTGGCGGCGGCGGCCGGC
GGGCTCGATGCGCTGGTGTTTACCGGCGGCATCGGCGAGCACGCCGCGCCGGTGCGCGAAAGGGTGGCACGACTGTCGGC
CTGGCTCGGTATCGACATCGACAGCGACGCCAACGCCCGCGACGCCAGCCGGCTGGACCGCGCCGGCAGCAAGGTGGCGG
TCGCGGTGGTGCCGACCAATGAGGAAGGCATGATCGCGCGCTACACGGTGGACGTGCTCGGCCTGTAA

Upstream 100 bases:

>100_bases
TGCCCATCGTGCTGACCAGCCGCGCCGACACCGCCGAAACCCGCGCCGCTTCGTGCGCGATCGCCCAGCTGATGGCGCAC
AAGAAGCGTGAGGCCAACAA

Downstream 100 bases:

>100_bases
TACCTGCGGCCGGCGCCTCAGCCGACGAGCGCATCCCACAGCAGCTTGAGGCCGGACAGGCCCAGCATTGCCGTGGAGAA
CAGGTAGAAAGGCCGTTCGG

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MKAVLVLNAGSSSLKFALFPIEPALADHAALSGQVEGIGATPEFSAKTAAGERFHETVPSNSDSQREQHREALTFLLDWL
QAHNPALEIAAAGHRIVHGGDKYSEPVQLYGHILRQLQKLVPLAPLHQPHNLNAVQAIAALAPDIPQVGCFDTAFHTTQA
PVAQAFALPRAISAEGVKRYGFHGLSYDYVARQLPAVIGDKASGAVVICHLGNGSSMCALRDGKSVASTMGFTALEGLVM
GTRTGSLDPGVLLYLMDEKGMDAKALTRLLYKESGLLGVSGISQDMRTLLASDAPEAAEAVDLYCYRIARELGSLAAAAG
GLDALVFTGGIGEHAAPVRERVARLSAWLGIDIDSDANARDASRLDRAGSKVAVAVVPTNEEGMIARYTVDVLGL

Sequences:

>Translated_395_residues
MKAVLVLNAGSSSLKFALFPIEPALADHAALSGQVEGIGATPEFSAKTAAGERFHETVPSNSDSQREQHREALTFLLDWL
QAHNPALEIAAAGHRIVHGGDKYSEPVQLYGHILRQLQKLVPLAPLHQPHNLNAVQAIAALAPDIPQVGCFDTAFHTTQA
PVAQAFALPRAISAEGVKRYGFHGLSYDYVARQLPAVIGDKASGAVVICHLGNGSSMCALRDGKSVASTMGFTALEGLVM
GTRTGSLDPGVLLYLMDEKGMDAKALTRLLYKESGLLGVSGISQDMRTLLASDAPEAAEAVDLYCYRIARELGSLAAAAG
GLDALVFTGGIGEHAAPVRERVARLSAWLGIDIDSDANARDASRLDRAGSKVAVAVVPTNEEGMIARYTVDVLGL
>Mature_395_residues
MKAVLVLNAGSSSLKFALFPIEPALADHAALSGQVEGIGATPEFSAKTAAGERFHETVPSNSDSQREQHREALTFLLDWL
QAHNPALEIAAAGHRIVHGGDKYSEPVQLYGHILRQLQKLVPLAPLHQPHNLNAVQAIAALAPDIPQVGCFDTAFHTTQA
PVAQAFALPRAISAEGVKRYGFHGLSYDYVARQLPAVIGDKASGAVVICHLGNGSSMCALRDGKSVASTMGFTALEGLVM
GTRTGSLDPGVLLYLMDEKGMDAKALTRLLYKESGLLGVSGISQDMRTLLASDAPEAAEAVDLYCYRIARELGSLAAAAG
GLDALVFTGGIGEHAAPVRERVARLSAWLGIDIDSDANARDASRLDRAGSKVAVAVVPTNEEGMIARYTVDVLGL

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI1788633, Length=399, Percent_Identity=39.5989974937343, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI145693184, Length=393, Percent_Identity=38.1679389312977, Blast_Score=249, Evalue=2e-67,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR004372 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.1 [H]

Molecular weight: Translated: 41606; Mature: 41606

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVLVLNAGSSSLKFALFPIEPALADHAALSGQVEGIGATPEFSAKTAAGERFHETVPS
CCEEEEEECCCCCEEEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
NSDSQREQHREALTFLLDWLQAHNPALEIAAAGHRIVHGGDKYSEPVQLYGHILRQLQKL
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEECCCCCCCHHHHHHHHHHHHHHH
VPLAPLHQPHNLNAVQAIAALAPDIPQVGCFDTAFHTTQAPVAQAFALPRAISAEGVKRY
CCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHCCHHHCHHHHHHH
GFHGLSYDYVARQLPAVIGDKASGAVVICHLGNGSSMCALRDGKSVASTMGFTALEGLVM
CCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHEEE
GTRTGSLDPGVLLYLMDEKGMDAKALTRLLYKESGLLGVSGISQDMRTLLASDAPEAAEA
ECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEECHHHHHHHHHHHCCCCHHHHH
VDLYCYRIARELGSLAAAAGGLDALVFTGGIGEHAAPVRERVARLSAWLGIDIDSDANAR
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHEEEECCCCCCH
DASRLDRAGSKVAVAVVPTNEEGMIARYTVDVLGL
HHHHHHHCCCEEEEEEEECCCCCEEEEEEEEECCC
>Mature Secondary Structure
MKAVLVLNAGSSSLKFALFPIEPALADHAALSGQVEGIGATPEFSAKTAAGERFHETVPS
CCEEEEEECCCCCEEEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
NSDSQREQHREALTFLLDWLQAHNPALEIAAAGHRIVHGGDKYSEPVQLYGHILRQLQKL
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEECCCCCCCHHHHHHHHHHHHHHH
VPLAPLHQPHNLNAVQAIAALAPDIPQVGCFDTAFHTTQAPVAQAFALPRAISAEGVKRY
CCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHCCCHHHHHHHHHCCHHHCHHHHHHH
GFHGLSYDYVARQLPAVIGDKASGAVVICHLGNGSSMCALRDGKSVASTMGFTALEGLVM
CCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHEEE
GTRTGSLDPGVLLYLMDEKGMDAKALTRLLYKESGLLGVSGISQDMRTLLASDAPEAAEA
ECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEECHHHHHHHHHHHCCCCHHHHH
VDLYCYRIARELGSLAAAAGGLDALVFTGGIGEHAAPVRERVARLSAWLGIDIDSDANAR
HHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHEEEECCCCCCH
DASRLDRAGSKVAVAVVPTNEEGMIARYTVDVLGL
HHHHHHHCCCEEEEEEEECCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA