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The map label for this gene is capD [H]

Identifier: 119899870

GI number: 119899870

Start: 3930274

End: 3932139

Strand: Reverse

Name: capD [H]

Synonym: azo3581

Alternate gene names: 119899870

Gene position: 3932139-3930274 (Counterclockwise)

Preceding gene: 119899871

Following gene: 119899869

Centisome position: 89.86

GC content: 67.1

Gene sequence:

>1866_bases
ATGCTGCTGCGTTTCAACTTCGAATGGCCTTCCGAGTACACCGGCAAGGCGCTTGCGTCTGTCGTGTTCCTGCTGTGCGT
CCAGGGCGCTGCATGTCGCTGGGCCGGTCTTTACCGCGGCATGTGGGTCTTCGCCAGCCTGCCCGACCTCAAACGCGTAT
TGAAGGCGATTGCCGTCTCCACGGTGGCCGTCACCGCACTCGTGGCACTCGACAGGGGGGCGCCGAACACGCCGCGCTCG
ATGCTGCTGCTCTACCCGATGCTCTTGCTGTTGGTGATGGGCGGGGGCCGGGCGGCGTGGCGGATGTGGAAGGAACACCG
GCTGTACGGCGGCCTCATCGGTGCGGGCAAGCCGGTCGTGGTGGTTGGCGCGGGAACCGGCGGGGCAATGCTGGTGCGCG
AGCTGCAGCGTAGCCCGGACTGGCGGGTGGTCGCGCTGGTGGATGATGATGCGAGCAAGTGGGGGCGCGAACTGTACGGC
TATCCGGTCGTTGGCGGCGTGGCCGATCTGCCGCAGGTGCTGGCGGACTACCGGGCCCAGCATGTGATCCTGGCGATGCC
GTCCGCGGCCAACAAGGCCATCCAGCGTGCTGCCGATACTGCGGTGCGGGCCGGGGCCCATGTCTTCAAGGTGCCGGGGC
TCGACGACCTGATGGGCGGCAAGGTGGCCGTCAGCGCGATGCGCCGGGTGGAAATCGAGGATCTGCTGGGGCGCGAGCCG
GTGCAGATCGACGACGCGAACGTGCAGCGCATGATCGCCGGCCGTACCGTGCTGATTACCGGGGCGGGGGGCTCCATCGG
CAGCGAACTGTGCCGCCAGGTGGCCCGTTTCCGCCCCAACCGGCTGGTGCTGTTCGATGTCAGCGAGTTCGCGCTGTACT
CCATCGAACAGTGGTTCTCGGTTTACCAGCCCGGGGTCAGCATCGTGCCGCTGGTGGGCGACGTGCGCGACGCGGCGCGG
CTGGACGAGGTATTCGCTTGTTACCGGCCGCAGCTGGTCTTTCATGCGGCGGCCTACAAGCATGTGCCGCTGATGGAAAT
CGACAACGCCTGGCAGGCGGTGCGCAACAACGTGCTGGGAACCCTGCTGGTGGCGGAGCACGCCTGCCGCTACGGGGCCG
AACGCTTCGTGCTGATCTCCACCGACAAGGCGGTCAATCCCACCAATGTGATGGGCGCCAGCAAGCGGCTTGCCGAAATG
GTGTGCCAGGCCCTGCAGGCCGAAAACGCCGCCAGCGGTGCTGCAACCCGGCTGGGGATGGTGCGCTTCGGCAACGTGCT
GGGCAGCACCGGCAGCGTGATTCCGAAGTTTGCGGAGCAGATCGCGCGCGGGGGGCCGGTAACCGTCACCCACCCCGAGA
TCAACCGCTACTTCATGTCGATTCCCGAGGCCGCCGAACTGGTGTTGCAGGCGGCGGCGATGGGGCAGGGCGGGGAGGTT
TTCGTGCTCGACATGGGCGAGCCGGTCAAGATCGTCGATCTCGCGCGCAACATGATCCGGCTGTCGGGTTACGACGAGGA
TGAGATCCGCATCGAGTTCACCGGGTTGCGGCCCGGCGAAAAACTCTACGAGGAATTGCTCGCGGACTCGGAGAGTACCT
GTCAGACGCCTCACCCCAAGCTGCGTATCGCGCGCTCGCCTGCGGTGGCGCCCGGCACGATCGCGGGTCTGCACGACTGG
TTGTCGCAGCCCGGCCCTGTCGCCGACGACGAGGTGAGGACGGCGCTGCACCACTGGGTGCCGGAATATGTGCCGGCACG
CTTGGCTGGAGAAGCCTCTGCCGCGCTGCAACTCGGTAGCCGCGGCGCACTGCTGGACGGCACGCTTTCACCGGCCATCG
CCGCGCGCGGCTCCATCCTGCATTAG

Upstream 100 bases:

>100_bases
AGAGCTTTCCCCCGCGCAAATAATGATTACGACCCCATCGCGCTCCATCCTCGTGCTCCTCTTCGATCTCGCCGCCGTCG
TGGTTGCGTGGGGCGTGGGC

Downstream 100 bases:

>100_bases
GCGGGCAGGGGCGCCGCCGGACGCCCAAGCCGCGGGAGAAACGCCCGGCTTTCTGACCGGCAGCGTGACGGCGTCGGTGT
CGCGTCGCAGCATATTGTTT

Product: putative capsular polysaccharide biosynthesis protein

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 621; Mature: 621

Protein sequence:

>621_residues
MLLRFNFEWPSEYTGKALASVVFLLCVQGAACRWAGLYRGMWVFASLPDLKRVLKAIAVSTVAVTALVALDRGAPNTPRS
MLLLYPMLLLLVMGGGRAAWRMWKEHRLYGGLIGAGKPVVVVGAGTGGAMLVRELQRSPDWRVVALVDDDASKWGRELYG
YPVVGGVADLPQVLADYRAQHVILAMPSAANKAIQRAADTAVRAGAHVFKVPGLDDLMGGKVAVSAMRRVEIEDLLGREP
VQIDDANVQRMIAGRTVLITGAGGSIGSELCRQVARFRPNRLVLFDVSEFALYSIEQWFSVYQPGVSIVPLVGDVRDAAR
LDEVFACYRPQLVFHAAAYKHVPLMEIDNAWQAVRNNVLGTLLVAEHACRYGAERFVLISTDKAVNPTNVMGASKRLAEM
VCQALQAENAASGAATRLGMVRFGNVLGSTGSVIPKFAEQIARGGPVTVTHPEINRYFMSIPEAAELVLQAAAMGQGGEV
FVLDMGEPVKIVDLARNMIRLSGYDEDEIRIEFTGLRPGEKLYEELLADSESTCQTPHPKLRIARSPAVAPGTIAGLHDW
LSQPGPVADDEVRTALHHWVPEYVPARLAGEASAALQLGSRGALLDGTLSPAIAARGSILH

Sequences:

>Translated_621_residues
MLLRFNFEWPSEYTGKALASVVFLLCVQGAACRWAGLYRGMWVFASLPDLKRVLKAIAVSTVAVTALVALDRGAPNTPRS
MLLLYPMLLLLVMGGGRAAWRMWKEHRLYGGLIGAGKPVVVVGAGTGGAMLVRELQRSPDWRVVALVDDDASKWGRELYG
YPVVGGVADLPQVLADYRAQHVILAMPSAANKAIQRAADTAVRAGAHVFKVPGLDDLMGGKVAVSAMRRVEIEDLLGREP
VQIDDANVQRMIAGRTVLITGAGGSIGSELCRQVARFRPNRLVLFDVSEFALYSIEQWFSVYQPGVSIVPLVGDVRDAAR
LDEVFACYRPQLVFHAAAYKHVPLMEIDNAWQAVRNNVLGTLLVAEHACRYGAERFVLISTDKAVNPTNVMGASKRLAEM
VCQALQAENAASGAATRLGMVRFGNVLGSTGSVIPKFAEQIARGGPVTVTHPEINRYFMSIPEAAELVLQAAAMGQGGEV
FVLDMGEPVKIVDLARNMIRLSGYDEDEIRIEFTGLRPGEKLYEELLADSESTCQTPHPKLRIARSPAVAPGTIAGLHDW
LSQPGPVADDEVRTALHHWVPEYVPARLAGEASAALQLGSRGALLDGTLSPAIAARGSILH
>Mature_621_residues
MLLRFNFEWPSEYTGKALASVVFLLCVQGAACRWAGLYRGMWVFASLPDLKRVLKAIAVSTVAVTALVALDRGAPNTPRS
MLLLYPMLLLLVMGGGRAAWRMWKEHRLYGGLIGAGKPVVVVGAGTGGAMLVRELQRSPDWRVVALVDDDASKWGRELYG
YPVVGGVADLPQVLADYRAQHVILAMPSAANKAIQRAADTAVRAGAHVFKVPGLDDLMGGKVAVSAMRRVEIEDLLGREP
VQIDDANVQRMIAGRTVLITGAGGSIGSELCRQVARFRPNRLVLFDVSEFALYSIEQWFSVYQPGVSIVPLVGDVRDAAR
LDEVFACYRPQLVFHAAAYKHVPLMEIDNAWQAVRNNVLGTLLVAEHACRYGAERFVLISTDKAVNPTNVMGASKRLAEM
VCQALQAENAASGAATRLGMVRFGNVLGSTGSVIPKFAEQIARGGPVTVTHPEINRYFMSIPEAAELVLQAAAMGQGGEV
FVLDMGEPVKIVDLARNMIRLSGYDEDEIRIEFTGLRPGEKLYEELLADSESTCQTPHPKLRIARSPAVAPGTIAGLHDW
LSQPGPVADDEVRTALHHWVPEYVPARLAGEASAALQLGSRGALLDGTLSPAIAARGSILH

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 67022; Mature: 67022

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLRFNFEWPSEYTGKALASVVFLLCVQGAACRWAGLYRGMWVFASLPDLKRVLKAIAVS
CEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
TVAVTALVALDRGAPNTPRSMLLLYPMLLLLVMGGGRAAWRMWKEHRLYGGLIGAGKPVV
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEECCCCCEE
VVGAGTGGAMLVRELQRSPDWRVVALVDDDASKWGRELYGYPVVGGVADLPQVLADYRAQ
EEECCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCC
HVILAMPSAANKAIQRAADTAVRAGAHVFKVPGLDDLMGGKVAVSAMRRVEIEDLLGREP
EEEEECCCHHHHHHHHHHHHHHHCCCEEEECCCCHHHCCCHHHHHHHHHHHHHHHHCCCC
VQIDDANVQRMIAGRTVLITGAGGSIGSELCRQVARFRPNRLVLFDVSEFALYSIEQWFS
CEECCCCHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHH
VYQPGVSIVPLVGDVRDAARLDEVFACYRPQLVFHAAAYKHVPLMEIDNAWQAVRNNVLG
HHCCCCEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHH
TLLVAEHACRYGAERFVLISTDKAVNPTNVMGASKRLAEMVCQALQAENAASGAATRLGM
HHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VRFGNVLGSTGSVIPKFAEQIARGGPVTVTHPEINRYFMSIPEAAELVLQAAAMGQGGEV
HHHHHHHCCCCCHHHHHHHHHHCCCCEEEECCHHHHHHHCCCHHHHHHHHHHHCCCCCCE
FVLDMGEPVKIVDLARNMIRLSGYDEDEIRIEFTGLRPGEKLYEELLADSESTCQTPHPK
EEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCC
LRIARSPAVAPGTIAGLHDWLSQPGPVADDEVRTALHHWVPEYVPARLAGEASAALQLGS
EEEECCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCHHHHHHHHCCCHHHHHHCCC
RGALLDGTLSPAIAARGSILH
CCCEECCCCCHHHHCCCCCCC
>Mature Secondary Structure
MLLRFNFEWPSEYTGKALASVVFLLCVQGAACRWAGLYRGMWVFASLPDLKRVLKAIAVS
CEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
TVAVTALVALDRGAPNTPRSMLLLYPMLLLLVMGGGRAAWRMWKEHRLYGGLIGAGKPVV
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCEECCCCCEE
VVGAGTGGAMLVRELQRSPDWRVVALVDDDASKWGRELYGYPVVGGVADLPQVLADYRAQ
EEECCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHCCC
HVILAMPSAANKAIQRAADTAVRAGAHVFKVPGLDDLMGGKVAVSAMRRVEIEDLLGREP
EEEEECCCHHHHHHHHHHHHHHHCCCEEEECCCCHHHCCCHHHHHHHHHHHHHHHHCCCC
VQIDDANVQRMIAGRTVLITGAGGSIGSELCRQVARFRPNRLVLFDVSEFALYSIEQWFS
CEECCCCHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHH
VYQPGVSIVPLVGDVRDAARLDEVFACYRPQLVFHAAAYKHVPLMEIDNAWQAVRNNVLG
HHCCCCEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEHHHHHHHHHHHHHH
TLLVAEHACRYGAERFVLISTDKAVNPTNVMGASKRLAEMVCQALQAENAASGAATRLGM
HHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VRFGNVLGSTGSVIPKFAEQIARGGPVTVTHPEINRYFMSIPEAAELVLQAAAMGQGGEV
HHHHHHHCCCCCHHHHHHHHHHCCCCEEEECCHHHHHHHCCCHHHHHHHHHHHCCCCCCE
FVLDMGEPVKIVDLARNMIRLSGYDEDEIRIEFTGLRPGEKLYEELLADSESTCQTPHPK
EEEECCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCC
LRIARSPAVAPGTIAGLHDWLSQPGPVADDEVRTALHHWVPEYVPARLAGEASAALQLGS
EEEECCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHCHHHHHHHHCCCHHHHHHCCC
RGALLDGTLSPAIAARGSILH
CCCEECCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]