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The map label for this gene is cobS [H]

Identifier: 119899850

GI number: 119899850

Start: 3907990

End: 3908760

Strand: Direct

Name: cobS [H]

Synonym: azo3561

Alternate gene names: 119899850

Gene position: 3907990-3908760 (Clockwise)

Preceding gene: 119899849

Following gene: 119899851

Centisome position: 89.3

GC content: 73.54

Gene sequence:

>771_bases
ATGCGGTCCCAGCTCGAACTCTTCTTCACCGCACTCGGTTACTTCAGCCGTCTGCCGGTGCCGGCCTGGGTGGCGTGGTC
GCCGCAGCGCGCCGCCGCGGCCGCGCGCTATTTCCCGCTGGTGGGCTGGATCGTCGGCGCCGCCGCGGCCGCAGCCTACG
CCCTGGCCGCGCTGGTACTGCCGCCCGCGCCCGCGGTGGTGATCGCGCTCGCAGCCGCGGTGCTGCTGACCGGCGCGCTG
CACGAGGACGGCTGGGCCGACGCCTGCGACGGTCTGGGCGGCGGCTGGGCGCGCGAACAGGTGCTGGCGATCATGAAGGA
CTCGCGCATCGGCAGCTACGGTGCGCTCGGCCTGATGCTGATGCTGCTCGCCAAAGCCGCCACGCTGGTCGAACTCGCCG
CCGCGGGCAGCGCCGCCTTCGCCAGCGCGCTGCTCGCCGCGCACGCGCTGTCGCGCCTTGCCGCCACCGCGCTGATGCAG
CGCCTGCCCTATGCGCGCAGCGACGACAGCAGCAAGAGCCGGCCGCTCGCGCAGCCGCTCGACCACGGCGGCGTCGCAAT
CGCCAGCGCGTGCGGCGTGTTGCCGCTGTTGCTGGTGCCCGGCGCGGCCGCCCTCGCGGCGCTGGCGACGACCGCGCTGC
TGACCTTTGCCGCGGCGCGCTACTTCCTCCGCCGTCTCGGCGGCTACACCGGCGATCTGCTCGGCGCCACCCAGCAGCTC
ACCGAACTCGGTTGCTACCTCGCCTTTCTCGCGACATGGAACTTCACCTGA

Upstream 100 bases:

>100_bases
CGCTGGCGTGGCCGCTGGTGCAGGCCGCGGTGAACTTCCTCGCCGAGATGGCGAGCTTCGAATCGGCCGGCGTCAGCGAC
AAGACCTGACCGCGGCGCCG

Downstream 100 bases:

>100_bases
TCCGCCACCCGCGCCCCGACGTGCCGGCCGGCGTGTGCTACGGCCAGCGCGACGTGGGCCTCGCCGAACCTGCCGCGACC
GTGGCCGCGCGCCTGCGGCC

Product: cobalamin synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MRSQLELFFTALGYFSRLPVPAWVAWSPQRAAAAARYFPLVGWIVGAAAAAAYALAALVLPPAPAVVIALAAAVLLTGAL
HEDGWADACDGLGGGWAREQVLAIMKDSRIGSYGALGLMLMLLAKAATLVELAAAGSAAFASALLAAHALSRLAATALMQ
RLPYARSDDSSKSRPLAQPLDHGGVAIASACGVLPLLLVPGAAALAALATTALLTFAAARYFLRRLGGYTGDLLGATQQL
TELGCYLAFLATWNFT

Sequences:

>Translated_256_residues
MRSQLELFFTALGYFSRLPVPAWVAWSPQRAAAAARYFPLVGWIVGAAAAAAYALAALVLPPAPAVVIALAAAVLLTGAL
HEDGWADACDGLGGGWAREQVLAIMKDSRIGSYGALGLMLMLLAKAATLVELAAAGSAAFASALLAAHALSRLAATALMQ
RLPYARSDDSSKSRPLAQPLDHGGVAIASACGVLPLLLVPGAAALAALATTALLTFAAARYFLRRLGGYTGDLLGATQQL
TELGCYLAFLATWNFT
>Mature_256_residues
MRSQLELFFTALGYFSRLPVPAWVAWSPQRAAAAARYFPLVGWIVGAAAAAAYALAALVLPPAPAVVIALAAAVLLTGAL
HEDGWADACDGLGGGWAREQVLAIMKDSRIGSYGALGLMLMLLAKAATLVELAAAGSAAFASALLAAHALSRLAATALMQ
RLPYARSDDSSKSRPLAQPLDHGGVAIASACGVLPLLLVPGAAALAALATTALLTFAAARYFLRRLGGYTGDLLGATQQL
TELGCYLAFLATWNFT

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=255, Percent_Identity=35.2941176470588, Blast_Score=119, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 26325; Mature: 26325

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSQLELFFTALGYFSRLPVPAWVAWSPQRAAAAARYFPLVGWIVGAAAAAAYALAALVL
CCCHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PPAPAVVIALAAAVLLTGALHEDGWADACDGLGGGWAREQVLAIMKDSRIGSYGALGLML
CCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
MLLAKAATLVELAAAGSAAFASALLAAHALSRLAATALMQRLPYARSDDSSKSRPLAQPL
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
DHGGVAIASACGVLPLLLVPGAAALAALATTALLTFAAARYFLRRLGGYTGDLLGATQQL
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
TELGCYLAFLATWNFT
HHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRSQLELFFTALGYFSRLPVPAWVAWSPQRAAAAARYFPLVGWIVGAAAAAAYALAALVL
CCCHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PPAPAVVIALAAAVLLTGALHEDGWADACDGLGGGWAREQVLAIMKDSRIGSYGALGLML
CCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
MLLAKAATLVELAAAGSAAFASALLAAHALSRLAATALMQRLPYARSDDSSKSRPLAQPL
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
DHGGVAIASACGVLPLLLVPGAAALAALATTALLTFAAARYFLRRLGGYTGDLLGATQQL
CCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
TELGCYLAFLATWNFT
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA