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The map label for this gene is rlmL [H]

Identifier: 119899766

GI number: 119899766

Start: 3817884

End: 3819047

Strand: Reverse

Name: rlmL [H]

Synonym: azo3477

Alternate gene names: 119899766

Gene position: 3819047-3817884 (Counterclockwise)

Preceding gene: 119899767

Following gene: 119899765

Centisome position: 87.27

GC content: 69.76

Gene sequence:

>1164_bases
ATGGCCGAAACGTTTTTTTCCCCCTGTCCGCGCGGTCTCGAAGCGTTGCTGGCCGACGAGTTGCGCGCACTCGGCGCGGC
CACGGCCGAGGCCGTGCATGGCGGCGTCAGCTGGTCGGGCGACTGGCAGGCCTGCTACCGCGCCAATCTCGAAAGCCGGC
TGGCGACGCGGGTGCTGTGGCGGGTGGGCAGCGGCCGCTATCGCGCCGAGGTGGATATCTACAAGCTGGCCTACAGCGTC
ACCTGGGCGAAGTGGTTTACGCCCGACGACACCATCCGGGTGTTCGTCACCGCGCAGAAGTCGCCGCTGAAAAGCCTGGA
GTTCATCACGCTGCGGATCAAGGACGCGGTGTGCGACCACTTCCGCACCGTGGCCGGCAAGCGGCCGAGCGTGGATACCG
CCAATCCCGCGGTGCGCATCCATGCCTTCCTGACCGCCGACACCGCCACGCTCTACATCGATACCTCGGGCGAACCGCTC
TACAAGCGCGGTTTCAAGCCGGCCGCGGTGGAGGCGCCGCTGAAGGAGAACCTGGCCGCCGGCATCCTGCTGCTGTCGGG
CTGGCGGCCTGATGAAGCCTTTGCCGATCCGATGTGCGGCAGCGGCACCTTCCTGCTCGAAGCCGCCCAGATGGCGCTGG
ACATCGCGCCCGGGCTGGGCCGGCGCTTCGCCTTCGAGCGCTTCAAGCATCTGGACCGGGCCGCGTGGGCGGCGCTGCGC
AAGGCGGCCGAGAACCGCCGTCAGCCCGCGCGGCCGCTGGCGGTGTACGGCTCCGACATCGTTGCCGACCAGGTGCGCCG
CAGCCGCAGCAATCTGGAGGCGGCGGGCCTTGCCGAGTGCGTGACGCTGGAGCGCGCCGACCTGCTCGAACGCGTCGCCC
CCGCCGCTGCCGGCGTCATGGTCACCAACCCGCCGTACGGGGTGCGGATCGGCGAGGCCGAGGAGCTGGCGGCGCTTTAT
CCGCGGCTGGGCGACGCCCTCAAGCGCAACTGGGCCGGCTGGCGCTGCCACTTCTTCAGCGCCGACGTGGCGCTGCCGAA
GCTGATCGGGCTCAAGGCAAGCCGGCGCACGCCGCTGTTCAATGGCGCGCTGGAATGCCGCCTGTACGAGTACCGAATGG
TGGCCGGCAGCGCGCGACGCGAGAAGGACGGCTCAAGTTCCTGA

Upstream 100 bases:

>100_bases
CGTTTTTCGTCCCGCTCCCGGCGCTGCGCCGCGGTGGCGCCCCGTGTCGCCGTCTTCCGCGAGGAGACCGCGCCCAATCC
GGTTGTCTGCGGAGTCTCCT

Downstream 100 bases:

>100_bases
AAACAGTGCCGAGAATTCCCGCAAAGGGAACAGGGTGAGGAGAACAGCGTGGCGAAGTACAAGGTGACACCGACAGGTGT
CGAACGACTGATGCGGGAGA

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MAETFFSPCPRGLEALLADELRALGAATAEAVHGGVSWSGDWQACYRANLESRLATRVLWRVGSGRYRAEVDIYKLAYSV
TWAKWFTPDDTIRVFVTAQKSPLKSLEFITLRIKDAVCDHFRTVAGKRPSVDTANPAVRIHAFLTADTATLYIDTSGEPL
YKRGFKPAAVEAPLKENLAAGILLLSGWRPDEAFADPMCGSGTFLLEAAQMALDIAPGLGRRFAFERFKHLDRAAWAALR
KAAENRRQPARPLAVYGSDIVADQVRRSRSNLEAAGLAECVTLERADLLERVAPAAAGVMVTNPPYGVRIGEAEELAALY
PRLGDALKRNWAGWRCHFFSADVALPKLIGLKASRRTPLFNGALECRLYEYRMVAGSARREKDGSSS

Sequences:

>Translated_387_residues
MAETFFSPCPRGLEALLADELRALGAATAEAVHGGVSWSGDWQACYRANLESRLATRVLWRVGSGRYRAEVDIYKLAYSV
TWAKWFTPDDTIRVFVTAQKSPLKSLEFITLRIKDAVCDHFRTVAGKRPSVDTANPAVRIHAFLTADTATLYIDTSGEPL
YKRGFKPAAVEAPLKENLAAGILLLSGWRPDEAFADPMCGSGTFLLEAAQMALDIAPGLGRRFAFERFKHLDRAAWAALR
KAAENRRQPARPLAVYGSDIVADQVRRSRSNLEAAGLAECVTLERADLLERVAPAAAGVMVTNPPYGVRIGEAEELAALY
PRLGDALKRNWAGWRCHFFSADVALPKLIGLKASRRTPLFNGALECRLYEYRMVAGSARREKDGSSS
>Mature_386_residues
AETFFSPCPRGLEALLADELRALGAATAEAVHGGVSWSGDWQACYRANLESRLATRVLWRVGSGRYRAEVDIYKLAYSVT
WAKWFTPDDTIRVFVTAQKSPLKSLEFITLRIKDAVCDHFRTVAGKRPSVDTANPAVRIHAFLTADTATLYIDTSGEPLY
KRGFKPAAVEAPLKENLAAGILLLSGWRPDEAFADPMCGSGTFLLEAAQMALDIAPGLGRRFAFERFKHLDRAAWAALRK
AAENRRQPARPLAVYGSDIVADQVRRSRSNLEAAGLAECVTLERADLLERVAPAAAGVMVTNPPYGVRIGEAEELAALYP
RLGDALKRNWAGWRCHFFSADVALPKLIGLKASRRTPLFNGALECRLYEYRMVAGSARREKDGSSS

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=378, Percent_Identity=34.9206349206349, Blast_Score=228, Evalue=5e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 42535; Mature: 42404

Theoretical pI: Translated: 9.32; Mature: 9.32

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE ; PS01261 UPF0020

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETFFSPCPRGLEALLADELRALGAATAEAVHGGVSWSGDWQACYRANLESRLATRVLW
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCHHHHHHHHHHH
RVGSGRYRAEVDIYKLAYSVTWAKWFTPDDTIRVFVTAQKSPLKSLEFITLRIKDAVCDH
HHCCCCEEEEEEEEEHHHHEEEEEECCCCCCEEEEEEECCCCCCCEEEEEEEEHHHHHHH
FRTVAGKRPSVDTANPAVRIHAFLTADTATLYIDTSGEPLYKRGFKPAAVEAPLKENLAA
HHHHCCCCCCCCCCCCEEEEEEEEEECCEEEEEECCCCHHHHCCCCCCCCCCCHHHHHHH
GILLLSGWRPDEAFADPMCGSGTFLLEAAQMALDIAPGLGRRFAFERFKHLDRAAWAALR
CEEEEECCCCCHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
KAAENRRQPARPLAVYGSDIVADQVRRSRSNLEAAGLAECVTLERADLLERVAPAAAGVM
HHHHHCCCCCCCEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEE
VTNPPYGVRIGEAEELAALYPRLGDALKRNWAGWRCHFFSADVALPKLIGLKASRRTPLF
EECCCCCEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHCCCCCCCCCCC
NGALECRLYEYRMVAGSARREKDGSSS
CCCEEEEEEEEEEHHCCCCCCCCCCCC
>Mature Secondary Structure 
AETFFSPCPRGLEALLADELRALGAATAEAVHGGVSWSGDWQACYRANLESRLATRVLW
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCHHHHHHHHHHH
RVGSGRYRAEVDIYKLAYSVTWAKWFTPDDTIRVFVTAQKSPLKSLEFITLRIKDAVCDH
HHCCCCEEEEEEEEEHHHHEEEEEECCCCCCEEEEEEECCCCCCCEEEEEEEEHHHHHHH
FRTVAGKRPSVDTANPAVRIHAFLTADTATLYIDTSGEPLYKRGFKPAAVEAPLKENLAA
HHHHCCCCCCCCCCCCEEEEEEEEEECCEEEEEECCCCHHHHCCCCCCCCCCCHHHHHHH
GILLLSGWRPDEAFADPMCGSGTFLLEAAQMALDIAPGLGRRFAFERFKHLDRAAWAALR
CEEEEECCCCCHHHCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
KAAENRRQPARPLAVYGSDIVADQVRRSRSNLEAAGLAECVTLERADLLERVAPAAAGVM
HHHHHCCCCCCCEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEE
VTNPPYGVRIGEAEELAALYPRLGDALKRNWAGWRCHFFSADVALPKLIGLKASRRTPLF
EECCCCCEEECCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHCCCCCCCCCCC
NGALECRLYEYRMVAGSARREKDGSSS
CCCEEEEEEEEEEHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA