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The map label for this gene is thiE

Identifier: 119899739

GI number: 119899739

Start: 3784828

End: 3785448

Strand: Reverse

Name: thiE

Synonym: azo3450

Alternate gene names: 119899739

Gene position: 3785448-3784828 (Counterclockwise)

Preceding gene: 119899740

Following gene: 119899738

Centisome position: 86.5

GC content: 70.05

Gene sequence:

>621_bases
ATGGGTGAGCGCATCCGCGGGCTTTATCTGGTGACGCCGGACGCCGCCGACAGCGCGACGCTGCTGGCGAGTGTGGAACG
CGTGCTGCCGGCCAGGCCGGCCTTGCTGCAGTACCGCAACAAGCTTGCCGACCCGCTCCGGCAACTCGACGAGGCGCGCT
CGTTGCGGCAGCTGTGCAGCAGCGCCGGCGTACCGCTGATCATCAACGATGACGTCGCGCTCGCCCGCGCGGTGGCCGCC
GACGGGGTGCATCTGGGCCGGGATGACGGCAGTCCCGCAGCCGCCAGGGCGGTCCTCGGCGCCGACGCGTTGATTGGCGT
CTCCTGCTACGACGAATGGGTGCGGGCGGAGGATGCGGCGGCAGCTGGCGCCGACTATGTGGCTTTTGGCGCAATGTTCC
CGTCGTCCACGAAACCCGGCGCGGTACGCGCGCCGCTGACGCTGTTGACCCGCGCCCGCGCGGCGCTCGACTGTCCGGTG
GCAGCCATTGGCGGCATCACCCTGGACAATGCACCGGCCTTGATCGAGGCCGGTGCCGATCTGCTTGCCGTCATATCGGA
TGTATTCGCCGCCGCCGACCCGCTGCAGCGCGCGCTCGCCTATTCGGCCCTGTTCGCCTGA

Upstream 100 bases:

>100_bases
CAGGATTTCGCCCAGCAGGCGCTCAAGCATGCCTACCGCGCGGGGATGGGGCTGGCGCTGCCCGACCGCTTCTTCTGGGC
GCGCGGAAAGGGCGTGGGCG

Downstream 100 bases:

>100_bases
AACTGCCCGCGGTGGACCGGCGCCGGTGGGCGCGCGACAATCCCGCCCTTTCCATAGCAGTCGCGAGTCGCAGCCATGAC
CAGTCGCAATGAAGCCCTTT

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MGERIRGLYLVTPDAADSATLLASVERVLPARPALLQYRNKLADPLRQLDEARSLRQLCSSAGVPLIINDDVALARAVAA
DGVHLGRDDGSPAAARAVLGADALIGVSCYDEWVRAEDAAAAGADYVAFGAMFPSSTKPGAVRAPLTLLTRARAALDCPV
AAIGGITLDNAPALIEAGADLLAVISDVFAAADPLQRALAYSALFA

Sequences:

>Translated_206_residues
MGERIRGLYLVTPDAADSATLLASVERVLPARPALLQYRNKLADPLRQLDEARSLRQLCSSAGVPLIINDDVALARAVAA
DGVHLGRDDGSPAAARAVLGADALIGVSCYDEWVRAEDAAAAGADYVAFGAMFPSSTKPGAVRAPLTLLTRARAALDCPV
AAIGGITLDNAPALIEAGADLLAVISDVFAAADPLQRALAYSALFA
>Mature_205_residues
GERIRGLYLVTPDAADSATLLASVERVLPARPALLQYRNKLADPLRQLDEARSLRQLCSSAGVPLIINDDVALARAVAAD
GVHLGRDDGSPAAARAVLGADALIGVSCYDEWVRAEDAAAAGADYVAFGAMFPSSTKPGAVRAPLTLLTRARAALDCPVA
AIGGITLDNAPALIEAGADLLAVISDVFAAADPLQRALAYSALFA

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=130, Percent_Identity=39.2307692307692, Blast_Score=71, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6325042, Length=208, Percent_Identity=31.7307692307692, Blast_Score=86, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_AZOSB (A1KB60)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_934952.1
- ProteinModelPortal:   A1KB60
- SMR:   A1KB60
- STRING:   A1KB60
- GeneID:   4608987
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo3450
- eggNOG:   COG0352
- HOGENOM:   HBG754477
- OMA:   CERYKTR
- ProtClustDB:   PRK00043
- BioCyc:   ASP62928:AZO3450-MONOMER
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 21204; Mature: 21073

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGERIRGLYLVTPDAADSATLLASVERVLPARPALLQYRNKLADPLRQLDEARSLRQLCS
CCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAGVPLIINDDVALARAVAADGVHLGRDDGSPAAARAVLGADALIGVSCYDEWVRAEDAA
HCCCCEEECCCHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAGADYVAFGAMFPSSTKPGAVRAPLTLLTRARAALDCPVAAIGGITLDNAPALIEAGAD
HCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCEEECCCCHHHHCCHH
LLAVISDVFAAADPLQRALAYSALFA
HHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GERIRGLYLVTPDAADSATLLASVERVLPARPALLQYRNKLADPLRQLDEARSLRQLCS
CCCCCEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAGVPLIINDDVALARAVAADGVHLGRDDGSPAAARAVLGADALIGVSCYDEWVRAEDAA
HCCCCEEECCCHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAGADYVAFGAMFPSSTKPGAVRAPLTLLTRARAALDCPVAAIGGITLDNAPALIEAGAD
HCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCEEECCCCHHHHCCHH
LLAVISDVFAAADPLQRALAYSALFA
HHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA