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The map label for this gene is rpoH [H]

Identifier: 119899581

GI number: 119899581

Start: 3631171

End: 3632025

Strand: Direct

Name: rpoH [H]

Synonym: azo3292

Alternate gene names: 119899581

Gene position: 3631171-3632025 (Clockwise)

Preceding gene: 119899578

Following gene: 119899587

Centisome position: 82.98

GC content: 65.26

Gene sequence:

>855_bases
ATGTCGCAAGCCTTGTCATTCCCTGTTCCGTCTGTTGTCGGCAGCATCGACCAATACATCCAGGCGGTGAATCGCGTGCC
CCTGCTGACGGAGCAGCAGGAGGTGGAACTCGCCACCCGCCTGCGTGACGACGGCGACGTCGATGCCGCACGCCAGTTGG
TGATGTCGCACCTGAGAGTGGTCGTGGCGATCGCGCGCGGCTATCTCGGTTACGGGCTGCCGCACGCCGACTTGATCCAG
GAAGGCAACATCGGCCTGATGAAGGCGGTGAAGCGCTTCGACCCGACCCGTGGGGTGCGGCTGGTGTCGTTCGCCATCCA
CTGGATCAAGGCCGAGATCCACGAGTACATCCTGAAAAACTGGCGGCTGGTGAAAATCGCCACCACCAAGGCGCAGCGCA
AGTTGTTCTTCAACCTGCGCAGCCTCAAGCAGGACACCGGCACGCTCAACCGCGACGAGGTGGAGGCGGTAGCCACCCAG
CTCGGCGTAAAGCCGGAAGAAGTGGTGGAGATGGAAACGCGGCTGAGCGGCCGCGACATTCCGATCGACGGCGGCAACGA
CGACGAGGACGAACGCTTCGCGCCGATCGCCTACCTGCCCGCCCCGGCCGCGGAACCTTCCGAGGTGCTGGAGCGCGCGG
AGCGGGCCCATCTGCAGGACACCGGGCTGAAGCAGGCGCTCGGCGCGCTCGACGAGCGCAGCCGCACCATCGTGGAACGC
CGCTGGCTTACCGACGGCGACAGCGCCACGCTGCACGAACTGGCGGCGGAGTACGGGGTGTCGGCAGAACGTATCCGCCA
GATCGAGGCGAAGGCAATGCAGAAAATGCGCGGTCTGCTGGCGCCGGCAATGTAA

Upstream 100 bases:

>100_bases
CTGGATCGAGCGAGCCTTGGGGCAGCGTAGGAACTCGCGTCAGCCCTTAGCACTCTCAAACACCGAGTGCTAAAATTGGC
GTCGAGGAGGACTTCAGCCC

Downstream 100 bases:

>100_bases
GCAGCGCAGCCGTATGCAAGAACCCGCGGCGGCCTTTTGGTCGCCGCGTTGCGTTTACCGTCGCGCCGTCGGCTCCGCGA
GCAACTGCTTGAGGTCGGCA

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MSQALSFPVPSVVGSIDQYIQAVNRVPLLTEQQEVELATRLRDDGDVDAARQLVMSHLRVVVAIARGYLGYGLPHADLIQ
EGNIGLMKAVKRFDPTRGVRLVSFAIHWIKAEIHEYILKNWRLVKIATTKAQRKLFFNLRSLKQDTGTLNRDEVEAVATQ
LGVKPEEVVEMETRLSGRDIPIDGGNDDEDERFAPIAYLPAPAAEPSEVLERAERAHLQDTGLKQALGALDERSRTIVER
RWLTDGDSATLHELAAEYGVSAERIRQIEAKAMQKMRGLLAPAM

Sequences:

>Translated_284_residues
MSQALSFPVPSVVGSIDQYIQAVNRVPLLTEQQEVELATRLRDDGDVDAARQLVMSHLRVVVAIARGYLGYGLPHADLIQ
EGNIGLMKAVKRFDPTRGVRLVSFAIHWIKAEIHEYILKNWRLVKIATTKAQRKLFFNLRSLKQDTGTLNRDEVEAVATQ
LGVKPEEVVEMETRLSGRDIPIDGGNDDEDERFAPIAYLPAPAAEPSEVLERAERAHLQDTGLKQALGALDERSRTIVER
RWLTDGDSATLHELAAEYGVSAERIRQIEAKAMQKMRGLLAPAM
>Mature_283_residues
SQALSFPVPSVVGSIDQYIQAVNRVPLLTEQQEVELATRLRDDGDVDAARQLVMSHLRVVVAIARGYLGYGLPHADLIQE
GNIGLMKAVKRFDPTRGVRLVSFAIHWIKAEIHEYILKNWRLVKIATTKAQRKLFFNLRSLKQDTGTLNRDEVEAVATQL
GVKPEEVVEMETRLSGRDIPIDGGNDDEDERFAPIAYLPAPAAEPSEVLERAERAHLQDTGLKQALGALDERSRTIVERR
WLTDGDSATLHELAAEYGVSAERIRQIEAKAMQKMRGLLAPAM

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=268, Percent_Identity=54.1044776119403, Blast_Score=290, Evalue=1e-79,
Organism=Escherichia coli, GI1789098, Length=278, Percent_Identity=33.8129496402878, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789448, Length=238, Percent_Identity=31.0924369747899, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 31697; Mature: 31566

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQALSFPVPSVVGSIDQYIQAVNRVPLLTEQQEVELATRLRDDGDVDAARQLVMSHLRV
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VVAIARGYLGYGLPHADLIQEGNIGLMKAVKRFDPTRGVRLVSFAIHWIKAEIHEYILKN
HHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHC
WRLVKIATTKAQRKLFFNLRSLKQDTGTLNRDEVEAVATQLGVKPEEVVEMETRLSGRDI
CEEEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCC
PIDGGNDDEDERFAPIAYLPAPAAEPSEVLERAERAHLQDTGLKQALGALDERSRTIVER
CCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
RWLTDGDSATLHELAAEYGVSAERIRQIEAKAMQKMRGLLAPAM
HHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SQALSFPVPSVVGSIDQYIQAVNRVPLLTEQQEVELATRLRDDGDVDAARQLVMSHLRV
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
VVAIARGYLGYGLPHADLIQEGNIGLMKAVKRFDPTRGVRLVSFAIHWIKAEIHEYILKN
HHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHC
WRLVKIATTKAQRKLFFNLRSLKQDTGTLNRDEVEAVATQLGVKPEEVVEMETRLSGRDI
CEEEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCC
PIDGGNDDEDERFAPIAYLPAPAAEPSEVLERAERAHLQDTGLKQALGALDERSRTIVER
CCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
RWLTDGDSATLHELAAEYGVSAERIRQIEAKAMQKMRGLLAPAM
HHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]