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The map label for this gene is pilB2 [H]

Identifier: 119899523

GI number: 119899523

Start: 3564593

End: 3566311

Strand: Direct

Name: pilB2 [H]

Synonym: azo3234

Alternate gene names: 119899523

Gene position: 3564593-3566311 (Clockwise)

Preceding gene: 119899522

Following gene: 119899524

Centisome position: 81.46

GC content: 62.77

Gene sequence:

>1719_bases
ATGGCAGCAAACCCCCAGGCCGCGCTGAGCGGGCTTGCCCGCGCACTAATCCAGCACGGTCGGCTTTCCGAAGCCGACGT
TCTCTCCTGTTCACCCAAAAGTACAGGGTCGAGCAACAGCTTCCTGCTTGAGATCGTGCAACGCGGCCTGATGACTGCCC
GCGACATCGCACGCTTTGCCGCCGAAACCTTCGGGTATCCGCTGTTCGACATCGCCGCCATTGACCGCGGTGCGATTCCC
CGCGACGCGGTCGATCGCAAGCTGATGGGCAAGCACCAAGTGGTGGCGCTGGGCAAGCGTCAGAACCGCCTCACCCTCGC
GGTCGCCGATCCGTCGAACATGCGCGTGCTCGACGAAATCCGGTTCCAGACCGGCATGCAGGTCGACCTCATCGTCGTCG
AGGTCGACAAGCTCTGCAAACTCGCCGACTCCCTCGCTGAATCCACCGAGCAGACGCTGAAGGATCTGACCGGCGGCGAA
GAGTTCGACATGGAGATGCTGCAGCAGGACGGCGGCACGGAGCAGTCCGCCGAGGACAGCACGGCGGAGGTCGACGACGC
ACCGATCGTCAAGTTCATCCAGAAGGTTCTTGTTGACGCGATCAATGAAGGCGCGTCCGACATCCATTTCGAGCCCTACG
AAAAGTACTACCGGATCCGCGTCCGTACTGACGGGATCTTGCGCGAGATTGCCCAGCCTCCCCTGGTGCTGAAGGAAAAG
ATCGCAGCGCGGATCAAGGTCATCTCGCGCCTCGACATCTCCGAGAAGCGGATTCCGCAGGACGGCCGCATGAAGCTCGT
CCTGTCAAAGACCAAGGCCATCGACTTCCGCGTATCCACGCTGCCGACGCTGCATGGCGAGAAGATCGTCATGCGTATTC
TCGACCCCAGTTCGGCGATGCTCGGCGTGGATGCGCTGGGCTACGAGCCCGAGCAGAAGAAGGCGCTGCTGGATGCGGTG
GAGCGTCCTTACGGCATGATCCTGGTCACGGGCCCCACGGGCTCGGGCAAGACGGTGTCGCTGTACACCTGCCTCAACCT
GCTCAACAAGGCCGGCGTCAATATTTCCACCGCGGAGGACCCGGCCGAAATCAATCTGCCCGGCATCAACCAGGTCAACG
TCAACGAGAAGGCGGGGCTCACCTTCGCCGCCTCGCTGCGCGCCTTCCTTCGCCAGGATCCGGACGTGATCATGGTCGGC
GAAATCCGCGACCTGGAAACCGCTGAAATCTCCGTCAAGGCAGCACAAACCGGCCACCTCGTGCTCTCCACGCTGCACAC
CAACGACGCCCCCACCACGCTCGAACGCCTGCGCAACATGGGGGTCGCGCCGTTCAACATCGCATCCTCGGTCATCATGA
TCACCGCGCAACGGCTCGCGCGCCGCCTGTGCACCTGCAAGAAGCCCGTCGATATCCCGATCGAAGCCTTGCTTCAGGCC
GGCTTCAAGGAACAGGATCTGGACGGCAGTTGGCAACCCTACGGCCCGGTCGGATGCGACCGCTGCAAGGGAAGCGGCTA
CAAGGGCCGGGTGGGCATCTACCAGGTCATGCCGGTGTCCGAGGAGATCGCCCACATCATCATGACCAACGGCAACTCGA
TGGACATCGCCGAGCAGGCGCGCCGCGAGGGCGTGAAGGATCTCCGCCAGTCCGGGCTTCTCAAGGTCAAGCAGGGCGTG
ACGTCACTGGAAGAAGTGCTCGCCTCCACCAACGAATAG

Upstream 100 bases:

>100_bases
TGGTGATATCCCGTCCGCTTGCTTCTCACGCTGCCCGCGCTCCCTTTACAAGGCCGGGAGCCAAGTGCAAGATCGCATGT
CAGTCAGTTAAGAGTGGCAC

Downstream 100 bases:

>100_bases
GCAAATCGGAAAACAACCCATGGCGACAGCGACGCGCGCCCCGCAGCGCACCACGACGGGCCCCAGGGAGGACCTCTACA
GCTGGGAAGGCAAGGACAAG

Product: pilus biogenesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 572; Mature: 571

Protein sequence:

>572_residues
MAANPQAALSGLARALIQHGRLSEADVLSCSPKSTGSSNSFLLEIVQRGLMTARDIARFAAETFGYPLFDIAAIDRGAIP
RDAVDRKLMGKHQVVALGKRQNRLTLAVADPSNMRVLDEIRFQTGMQVDLIVVEVDKLCKLADSLAESTEQTLKDLTGGE
EFDMEMLQQDGGTEQSAEDSTAEVDDAPIVKFIQKVLVDAINEGASDIHFEPYEKYYRIRVRTDGILREIAQPPLVLKEK
IAARIKVISRLDISEKRIPQDGRMKLVLSKTKAIDFRVSTLPTLHGEKIVMRILDPSSAMLGVDALGYEPEQKKALLDAV
ERPYGMILVTGPTGSGKTVSLYTCLNLLNKAGVNISTAEDPAEINLPGINQVNVNEKAGLTFAASLRAFLRQDPDVIMVG
EIRDLETAEISVKAAQTGHLVLSTLHTNDAPTTLERLRNMGVAPFNIASSVIMITAQRLARRLCTCKKPVDIPIEALLQA
GFKEQDLDGSWQPYGPVGCDRCKGSGYKGRVGIYQVMPVSEEIAHIIMTNGNSMDIAEQARREGVKDLRQSGLLKVKQGV
TSLEEVLASTNE

Sequences:

>Translated_572_residues
MAANPQAALSGLARALIQHGRLSEADVLSCSPKSTGSSNSFLLEIVQRGLMTARDIARFAAETFGYPLFDIAAIDRGAIP
RDAVDRKLMGKHQVVALGKRQNRLTLAVADPSNMRVLDEIRFQTGMQVDLIVVEVDKLCKLADSLAESTEQTLKDLTGGE
EFDMEMLQQDGGTEQSAEDSTAEVDDAPIVKFIQKVLVDAINEGASDIHFEPYEKYYRIRVRTDGILREIAQPPLVLKEK
IAARIKVISRLDISEKRIPQDGRMKLVLSKTKAIDFRVSTLPTLHGEKIVMRILDPSSAMLGVDALGYEPEQKKALLDAV
ERPYGMILVTGPTGSGKTVSLYTCLNLLNKAGVNISTAEDPAEINLPGINQVNVNEKAGLTFAASLRAFLRQDPDVIMVG
EIRDLETAEISVKAAQTGHLVLSTLHTNDAPTTLERLRNMGVAPFNIASSVIMITAQRLARRLCTCKKPVDIPIEALLQA
GFKEQDLDGSWQPYGPVGCDRCKGSGYKGRVGIYQVMPVSEEIAHIIMTNGNSMDIAEQARREGVKDLRQSGLLKVKQGV
TSLEEVLASTNE
>Mature_571_residues
AANPQAALSGLARALIQHGRLSEADVLSCSPKSTGSSNSFLLEIVQRGLMTARDIARFAAETFGYPLFDIAAIDRGAIPR
DAVDRKLMGKHQVVALGKRQNRLTLAVADPSNMRVLDEIRFQTGMQVDLIVVEVDKLCKLADSLAESTEQTLKDLTGGEE
FDMEMLQQDGGTEQSAEDSTAEVDDAPIVKFIQKVLVDAINEGASDIHFEPYEKYYRIRVRTDGILREIAQPPLVLKEKI
AARIKVISRLDISEKRIPQDGRMKLVLSKTKAIDFRVSTLPTLHGEKIVMRILDPSSAMLGVDALGYEPEQKKALLDAVE
RPYGMILVTGPTGSGKTVSLYTCLNLLNKAGVNISTAEDPAEINLPGINQVNVNEKAGLTFAASLRAFLRQDPDVIMVGE
IRDLETAEISVKAAQTGHLVLSTLHTNDAPTTLERLRNMGVAPFNIASSVIMITAQRLARRLCTCKKPVDIPIEALLQAG
FKEQDLDGSWQPYGPVGCDRCKGSGYKGRVGIYQVMPVSEEIAHIIMTNGNSMDIAEQARREGVKDLRQSGLLKVKQGVT
SLEEVLASTNE

Specific function: Involved in the translocation of the type IV pilin (pilA) [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=394, Percent_Identity=49.238578680203, Blast_Score=345, Evalue=5e-96,
Organism=Escherichia coli, GI1786296, Length=486, Percent_Identity=37.8600823045267, Blast_Score=325, Evalue=7e-90,
Organism=Escherichia coli, GI87082188, Length=250, Percent_Identity=32, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 62432; Mature: 62300

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAANPQAALSGLARALIQHGRLSEADVLSCSPKSTGSSNSFLLEIVQRGLMTARDIARFA
CCCCHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AETFGYPLFDIAAIDRGAIPRDAVDRKLMGKHQVVALGKRQNRLTLAVADPSNMRVLDEI
HHHHCCCHHHHHHCCCCCCCHHHHHHHHCCCCCEEEECCCCCEEEEEEECCCCCHHHHHH
RFQTGMQVDLIVVEVDKLCKLADSLAESTEQTLKDLTGGEEFDMEMLQQDGGTEQSAEDS
HHHCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCC
TAEVDDAPIVKFIQKVLVDAINEGASDIHFEPYEKYYRIRVRTDGILREIAQPPLVLKEK
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCHHHHHHCCCCHHHHHH
IAARIKVISRLDISEKRIPQDGRMKLVLSKTKAIDFRVSTLPTLHGEKIVMRILDPSSAM
HHHHHHHHHHCCCHHHHCCCCCCEEEEEEECCEEEEEECCCCCCCCCEEEEEEECCCCCE
LGVDALGYEPEQKKALLDAVERPYGMILVTGPTGSGKTVSLYTCLNLLNKAGVNISTAED
ECCHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCEEHHHHHHHHHHHCCCEEECCCC
PAEINLPGINQVNVNEKAGLTFAASLRAFLRQDPDVIMVGEIRDLETAEISVKAAQTGHL
CCEECCCCCCEECCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECCCCCE
VLSTLHTNDAPTTLERLRNMGVAPFNIASSVIMITAQRLARRLCTCKKPVDIPIEALLQA
EEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHC
GFKEQDLDGSWQPYGPVGCDRCKGSGYKGRVGIYQVMPVSEEIAHIIMTNGNSMDIAEQA
CCCCCCCCCCCCCCCCCCCHHCCCCCCCCCEEEEEECCCHHHHEEEEEECCCCCCHHHHH
RREGVKDLRQSGLLKVKQGVTSLEEVLASTNE
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
AANPQAALSGLARALIQHGRLSEADVLSCSPKSTGSSNSFLLEIVQRGLMTARDIARFA
CCCHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AETFGYPLFDIAAIDRGAIPRDAVDRKLMGKHQVVALGKRQNRLTLAVADPSNMRVLDEI
HHHHCCCHHHHHHCCCCCCCHHHHHHHHCCCCCEEEECCCCCEEEEEEECCCCCHHHHHH
RFQTGMQVDLIVVEVDKLCKLADSLAESTEQTLKDLTGGEEFDMEMLQQDGGTEQSAEDS
HHHCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCC
TAEVDDAPIVKFIQKVLVDAINEGASDIHFEPYEKYYRIRVRTDGILREIAQPPLVLKEK
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCHHHHHHCCCCHHHHHH
IAARIKVISRLDISEKRIPQDGRMKLVLSKTKAIDFRVSTLPTLHGEKIVMRILDPSSAM
HHHHHHHHHHCCCHHHHCCCCCCEEEEEEECCEEEEEECCCCCCCCCEEEEEEECCCCCE
LGVDALGYEPEQKKALLDAVERPYGMILVTGPTGSGKTVSLYTCLNLLNKAGVNISTAED
ECCHHCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCEEHHHHHHHHHHHCCCEEECCCC
PAEINLPGINQVNVNEKAGLTFAASLRAFLRQDPDVIMVGEIRDLETAEISVKAAQTGHL
CCEECCCCCCEECCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEECCCCCE
VLSTLHTNDAPTTLERLRNMGVAPFNIASSVIMITAQRLARRLCTCKKPVDIPIEALLQA
EEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHC
GFKEQDLDGSWQPYGPVGCDRCKGSGYKGRVGIYQVMPVSEEIAHIIMTNGNSMDIAEQA
CCCCCCCCCCCCCCCCCCCHHCCCCCCCCCEEEEEECCCHHHHEEEEEECCCCCCHHHHH
RREGVKDLRQSGLLKVKQGVTSLEEVLASTNE
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1971619; 10984043 [H]