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The map label for this gene is thyA

Identifier: 119899486

GI number: 119899486

Start: 3518714

End: 3519508

Strand: Direct

Name: thyA

Synonym: azo3197

Alternate gene names: 119899486

Gene position: 3518714-3519508 (Clockwise)

Preceding gene: 119899484

Following gene: 119899487

Centisome position: 80.41

GC content: 62.77

Gene sequence:

>795_bases
ATGCAGCAATACCTCGACCTCATGCGGCATGTCCTTGAGCACGGGGACCGCAAGACGGACCGGACAGGCACGGGAACGGT
GTCGGTGTTCGGCTGGCAGATGCGGTTCCGCCTTGCCGAAGGCTTTCCGCTACTCACCACCAAGAAACTCCACACCCGCT
CGATCATCCACGAATTGCTGTGGTTCATTCGGGGCGATACGAACATCCGGTACCTGAAGGACCATGGCGTTTCGATCTGG
GACGAATGGGCCGACGAGAACGGGGATCTCGGACCCGTTTACGGAAAGCAGTGGCGTCGCTGGGAGACCGCCGACGGACG
GAGCGTGGACCAGCTCGCGCAGCTGATCGACGGCATCAAGCGCAACCCCGACTCGCGCCGGCACATCGTGTCGGCATGGA
ACCCGGGCGAAGTCGGCAGCATGGCCCTGCCGCCGTGCCACGCGCTGTTCCAGTTCTACGTCGCCGGCGGCCGGCTGTCC
TGCCAGCTTTATCAGCGCAGTGCCGACATCTTCCTCGGCGTGCCCTTCAATATTGCGTCCTACGCCCTGCTCACAATGAT
GGTGGCGCAGGTGTGCGACCTTGAGCCTGGCGACTTCGTGTGGACGGGCGGAGATTGCCATCTCTATCTCAACCATTTGC
AGCAAGCCGAGGAGCAGCTCCAGCGCACGCCCTACCCCTTGCCGCGGATGATCCTGAACCCCGAGGTGCGCGACCTGTTC
GCATTCCGCTTCGAAGACTTCACGCTGGAGGGCTACACCGCCCATCCGCACATCAAGGCGCCTGTCGCAGTATGA

Upstream 100 bases:

>100_bases
TTTAAGTCATAAAGCACCGCCCGGGTACGGCGGGCCGCGGCAGCGACCTCGCGATTTCCACGCCTTCCGCAGTTAGAATC
AAGGACTTTTGGAGCGCCTC

Downstream 100 bases:

>100_bases
CGACTCCACGCTCCCTCACCTTCGTCGTCGCGATCGCCTCCAACGGCGTGATCGGCCGAAACAACGCGCTGCCCTGGCGG
CTGAAGGCCGACCTGGCGCA

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MQQYLDLMRHVLEHGDRKTDRTGTGTVSVFGWQMRFRLAEGFPLLTTKKLHTRSIIHELLWFIRGDTNIRYLKDHGVSIW
DEWADENGDLGPVYGKQWRRWETADGRSVDQLAQLIDGIKRNPDSRRHIVSAWNPGEVGSMALPPCHALFQFYVAGGRLS
CQLYQRSADIFLGVPFNIASYALLTMMVAQVCDLEPGDFVWTGGDCHLYLNHLQQAEEQLQRTPYPLPRMILNPEVRDLF
AFRFEDFTLEGYTAHPHIKAPVAV

Sequences:

>Translated_264_residues
MQQYLDLMRHVLEHGDRKTDRTGTGTVSVFGWQMRFRLAEGFPLLTTKKLHTRSIIHELLWFIRGDTNIRYLKDHGVSIW
DEWADENGDLGPVYGKQWRRWETADGRSVDQLAQLIDGIKRNPDSRRHIVSAWNPGEVGSMALPPCHALFQFYVAGGRLS
CQLYQRSADIFLGVPFNIASYALLTMMVAQVCDLEPGDFVWTGGDCHLYLNHLQQAEEQLQRTPYPLPRMILNPEVRDLF
AFRFEDFTLEGYTAHPHIKAPVAV
>Mature_264_residues
MQQYLDLMRHVLEHGDRKTDRTGTGTVSVFGWQMRFRLAEGFPLLTTKKLHTRSIIHELLWFIRGDTNIRYLKDHGVSIW
DEWADENGDLGPVYGKQWRRWETADGRSVDQLAQLIDGIKRNPDSRRHIVSAWNPGEVGSMALPPCHALFQFYVAGGRLS
CQLYQRSADIFLGVPFNIASYALLTMMVAQVCDLEPGDFVWTGGDCHLYLNHLQQAEEQLQRTPYPLPRMILNPEVRDLF
AFRFEDFTLEGYTAHPHIKAPVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily

Homologues:

Organism=Homo sapiens, GI4507751, Length=282, Percent_Identity=52.4822695035461, Blast_Score=291, Evalue=6e-79,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=67.0454545454545, Blast_Score=386, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI71993377, Length=283, Percent_Identity=46.9964664310954, Blast_Score=262, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6324648, Length=292, Percent_Identity=40.0684931506849, Blast_Score=206, Evalue=2e-54,
Organism=Drosophila melanogaster, GI17137556, Length=283, Percent_Identity=53.356890459364, Blast_Score=297, Evalue=5e-81,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): TYSY_AZOSB (A1KAF7)

Other databases:

- EMBL:   AM406670
- RefSeq:   YP_934699.1
- ProteinModelPortal:   A1KAF7
- SMR:   A1KAF7
- STRING:   A1KAF7
- GeneID:   4607846
- GenomeReviews:   AM406670_GR
- KEGG:   azo:azo3197
- eggNOG:   COG0207
- HOGENOM:   HBG588098
- OMA:   RMALAPC
- PhylomeDB:   A1KAF7
- ProtClustDB:   PRK01827
- BioCyc:   ASP62928:AZO3197-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00008
- InterPro:   IPR000398
- InterPro:   IPR020940
- Gene3D:   G3DSA:3.30.572.10
- PANTHER:   PTHR11549:SF2
- PRINTS:   PR00108
- TIGRFAMs:   TIGR03284

Pfam domain/function: PF00303 Thymidylat_synt; SSF55831 Thymidylat_synth_C

EC number: =2.1.1.45

Molecular weight: Translated: 30371; Mature: 30371

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: ACT_SITE 146-146

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQYLDLMRHVLEHGDRKTDRTGTGTVSVFGWQMRFRLAEGFPLLTTKKLHTRSIIHELL
CHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHEEEEEHHCCCCCEECHHHHHHHHHHHHH
WFIRGDTNIRYLKDHGVSIWDEWADENGDLGPVYGKQWRRWETADGRSVDQLAQLIDGIK
HHHCCCCCEEEEECCCCCHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHH
RNPDSRRHIVSAWNPGEVGSMALPPCHALFQFYVAGGRLSCQLYQRSADIFLGVPFNIAS
CCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCEEEECCHHHHH
YALLTMMVAQVCDLEPGDFVWTGGDCHLYLNHLQQAEEQLQRTPYPLPRMILNPEVRDLF
HHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCHHHHH
AFRFEDFTLEGYTAHPHIKAPVAV
HHHHCCEEEECEECCCCCCCCCCC
>Mature Secondary Structure
MQQYLDLMRHVLEHGDRKTDRTGTGTVSVFGWQMRFRLAEGFPLLTTKKLHTRSIIHELL
CHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHEEEEEHHCCCCCEECHHHHHHHHHHHHH
WFIRGDTNIRYLKDHGVSIWDEWADENGDLGPVYGKQWRRWETADGRSVDQLAQLIDGIK
HHHCCCCCEEEEECCCCCHHHHHCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHH
RNPDSRRHIVSAWNPGEVGSMALPPCHALFQFYVAGGRLSCQLYQRSADIFLGVPFNIAS
CCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCEEEECCHHHHH
YALLTMMVAQVCDLEPGDFVWTGGDCHLYLNHLQQAEEQLQRTPYPLPRMILNPEVRDLF
HHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCHHHHH
AFRFEDFTLEGYTAHPHIKAPVAV
HHHHCCEEEECEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA