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The map label for this gene is folA [H]

Identifier: 119899487

GI number: 119899487

Start: 3519505

End: 3519999

Strand: Direct

Name: folA [H]

Synonym: azo3198

Alternate gene names: 119899487

Gene position: 3519505-3519999 (Clockwise)

Preceding gene: 119899486

Following gene: 119899491

Centisome position: 80.43

GC content: 65.25

Gene sequence:

>495_bases
ATGACGACTCCACGCTCCCTCACCTTCGTCGTCGCGATCGCCTCCAACGGCGTGATCGGCCGAAACAACGCGCTGCCCTG
GCGGCTGAAGGCCGACCTGGCGCACTTCAAGGCTGTCACCCTGGGTCACCCGATCGTGATGGGTCGCAAGACCTGGGAAT
CGCTCGGCCGCCCATTGCCGGGTCGGCGCAACATGGTGGTCACGCGCAACGCGGATTACCGGGCGATCGGCGCGGAGGTC
TTTGCGACGCCGGAGGCCGCGGTTAATGCGGTGGCGGAGGGCCCCCTGTTCGTGATCGGAGGCGCCCAGTTGTACAAGGA
ACTGCTGCCGATGGCTGACCGGCTGCTGATCACCGAGGTCCTCGCCGACGTAGAAGGCGATGCCTACTTTCCGCCCTTCA
ACCTCGACACATTCCGCGAATGCAGCCGCGAACACCACCCCGCGGATGCCGACAACCAATTCGCGTTCGACATCGTCGAC
TACCGCCGCCGCTAA

Upstream 100 bases:

>100_bases
TCCTGAACCCCGAGGTGCGCGACCTGTTCGCATTCCGCTTCGAAGACTTCACGCTGGAGGGCTACACCGCCCATCCGCAC
ATCAAGGCGCCTGTCGCAGT

Downstream 100 bases:

>100_bases
ACGACTACGGCCCCTAGGGGGCCGTAGTGTCTGCACCGATACCGATCTAGTCTGCCGCTACGCAATCCACGTAGTAACGG
CCGTCGTCTCCCTTCACCAG

Product: FolA protein

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 164; Mature: 163

Protein sequence:

>164_residues
MTTPRSLTFVVAIASNGVIGRNNALPWRLKADLAHFKAVTLGHPIVMGRKTWESLGRPLPGRRNMVVTRNADYRAIGAEV
FATPEAAVNAVAEGPLFVIGGAQLYKELLPMADRLLITEVLADVEGDAYFPPFNLDTFRECSREHHPADADNQFAFDIVD
YRRR

Sequences:

>Translated_164_residues
MTTPRSLTFVVAIASNGVIGRNNALPWRLKADLAHFKAVTLGHPIVMGRKTWESLGRPLPGRRNMVVTRNADYRAIGAEV
FATPEAAVNAVAEGPLFVIGGAQLYKELLPMADRLLITEVLADVEGDAYFPPFNLDTFRECSREHHPADADNQFAFDIVD
YRRR
>Mature_163_residues
TTPRSLTFVVAIASNGVIGRNNALPWRLKADLAHFKAVTLGHPIVMGRKTWESLGRPLPGRRNMVVTRNADYRAIGAEVF
ATPEAAVNAVAEGPLFVIGGAQLYKELLPMADRLLITEVLADVEGDAYFPPFNLDTFRECSREHHPADADNQFAFDIVDY
RRR

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=155, Percent_Identity=29.6774193548387, Blast_Score=77, Evalue=9e-15,
Organism=Escherichia coli, GI1786233, Length=160, Percent_Identity=41.875, Blast_Score=130, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17505899, Length=183, Percent_Identity=28.4153005464481, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324810, Length=123, Percent_Identity=30.0813008130081, Blast_Score=64, Evalue=9e-12,
Organism=Drosophila melanogaster, GI116008026, Length=152, Percent_Identity=30.9210526315789, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24647458, Length=152, Percent_Identity=30.9210526315789, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18206; Mature: 18075

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPRSLTFVVAIASNGVIGRNNALPWRLKADLAHFKAVTLGHPIVMGRKTWESLGRPLP
CCCCCCEEEEEEEECCCEEECCCCCCEEEHHHHHHHHHHHCCCCEEECCHHHHHHCCCCC
GRRNMVVTRNADYRAIGAEVFATPEAAVNAVAEGPLFVIGGAQLYKELLPMADRLLITEV
CCCCEEEEECCCCEEECCEEEECCHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHH
LADVEGDAYFPPFNLDTFRECSREHHPADADNQFAFDIVDYRRR
HHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEHHHHCCC
>Mature Secondary Structure 
TTPRSLTFVVAIASNGVIGRNNALPWRLKADLAHFKAVTLGHPIVMGRKTWESLGRPLP
CCCCCEEEEEEEECCCEEECCCCCCEEEHHHHHHHHHHHCCCCEEECCHHHHHHCCCCC
GRRNMVVTRNADYRAIGAEVFATPEAAVNAVAEGPLFVIGGAQLYKELLPMADRLLITEV
CCCCEEEEECCCCEEECCEEEECCHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHH
LADVEGDAYFPPFNLDTFRECSREHHPADADNQFAFDIVDYRRR
HHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]