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The map label for this gene is capL [H]

Identifier: 119899479

GI number: 119899479

Start: 3501801

End: 3503081

Strand: Reverse

Name: capL [H]

Synonym: azo3189

Alternate gene names: 119899479

Gene position: 3503081-3501801 (Counterclockwise)

Preceding gene: 119899480

Following gene: 119899478

Centisome position: 80.05

GC content: 62.06

Gene sequence:

>1281_bases
ATGACAACCATTGCTGTCATCGGCCTGGGCTATGTAGGGCTGCCGCTTGCGGTAGCGTTCGGCAAACAGTTCCGGACGAT
CGGGTTCGACCTCTCCGCCGAGAAGGTCGCCGCGTACAAGGATTTCATCGATCCCACCGGTGAAGTGTCCAGTGAAGAAC
TCAAGGCCGCGGAGCATCTCAGCTGCCATACCGACCCGGCTGTCCTGCGCGAGGCGGATTTCGTCATCGTCGCGGTGCCC
ACTCCGGTCGACGAGGCCCACAACCCCGATTTCACACCGCTGGTGAAGTCTTCCGAGACGGTCGGCCGCAACCTCAAGCC
GGGTGCGATCGTCGTCTACGAATCCACGGTTTACCCCGGGGCGACCGAGGAGGTCTGCATTCCCATCCTCGAGCGGGAAT
CCGGCCTCAAGTGGAAGGAAGGTTTCTTCGTGGGCTACTCGCCCGAACGGATCAACCCGGGCGACAAGGAACGCACCGTC
ACCAAGATCGTGAAGGTCGTATCGGGCGATACGCCCGAGACCCTGGCGACGGTGGAACGGGTGTATGGCGCCGTAATCAC
CGCAGGGGTGTATCCGGCAAGCTCGATCAAGGTGGCCGAAGCCGCTAAGGTTATCGAGAACACCCAGCGCGACCTCAACA
TCGCGCTGATGAATGAACTGGCGGTCATCTTCCACAAGATCGGCATCGACACCCTTGAAGTGCTGAAGGCAGCTGGCACG
AAGTGGAACTTCCTGCCCTTCCGCCCCGGTCTGGTCGGCGGTCATTGCATCGGCGTCGATCCTTACTATCTGACCTACAA
GGCCGACATGCTCGGCTATCACCCGCAGGTCATCCTCGCGGGGCGTCGGATCAATGACGGGATGGGCAAGTATGTGGCCG
AGCAGACGGTGAAGCAGATGATCGCCGCGGGCTCCAGCATCAAGGGGGCGAAGGTGGTCGTTCTCGGCCTGACCTTCAAG
GAAAACTGCCCTGACCTGCGCAACAGCAAGGTCATCGATGTGATCCGCGAATTGCAGAGCTATGGCTGCGACGTGATCGT
CCACGACCCGGTCGCCGCTGCCGCCGAGGCGGTGCACGAATACGGCGTCGACCTCGTTGCATGGGACGCGCTGCCGACTG
CGGAAGCCGTTGTGGCGGCTGTCTCGCATCGCGAGTACCTGGACATGCCGCTTGCGCAGATGCTGGGCAAGCTGAAGCCG
GGAGGCGTGTTTGTCGACGTGAAGTCAGCCTACGACCCTGCCGAGCTCACAGCCGCAGGCGCGCGGCTGTGGCGTCTGTA
A

Upstream 100 bases:

>100_bases
GCCGAAGTTGCAGCAGGGGACGCCGCTGTACGGTGAGGCGACCACGTTGATGAAGAGTCTCTAACTACACCGGATTTTGT
TTCGATCAAGGAAGCGTCTC

Downstream 100 bases:

>100_bases
GACGGCCGCGCTGATGAAACCGCGCCCGCCTCTGCCGCTTGCCCGCCGGCATGCGCTGGCGGGCCTGATCGTCGCGTGTA
CAGCCGCCGCACAGCCGGCG

Product: NDPsugar dehydrogenase

Products: UDP-N-acetyl-D-mannosaminuronic-acid; NADH; Proton [C]

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTTIAVIGLGYVGLPLAVAFGKQFRTIGFDLSAEKVAAYKDFIDPTGEVSSEELKAAEHLSCHTDPAVLREADFVIVAVP
TPVDEAHNPDFTPLVKSSETVGRNLKPGAIVVYESTVYPGATEEVCIPILERESGLKWKEGFFVGYSPERINPGDKERTV
TKIVKVVSGDTPETLATVERVYGAVITAGVYPASSIKVAEAAKVIENTQRDLNIALMNELAVIFHKIGIDTLEVLKAAGT
KWNFLPFRPGLVGGHCIGVDPYYLTYKADMLGYHPQVILAGRRINDGMGKYVAEQTVKQMIAAGSSIKGAKVVVLGLTFK
ENCPDLRNSKVIDVIRELQSYGCDVIVHDPVAAAAEAVHEYGVDLVAWDALPTAEAVVAAVSHREYLDMPLAQMLGKLKP
GGVFVDVKSAYDPAELTAAGARLWRL

Sequences:

>Translated_426_residues
MTTIAVIGLGYVGLPLAVAFGKQFRTIGFDLSAEKVAAYKDFIDPTGEVSSEELKAAEHLSCHTDPAVLREADFVIVAVP
TPVDEAHNPDFTPLVKSSETVGRNLKPGAIVVYESTVYPGATEEVCIPILERESGLKWKEGFFVGYSPERINPGDKERTV
TKIVKVVSGDTPETLATVERVYGAVITAGVYPASSIKVAEAAKVIENTQRDLNIALMNELAVIFHKIGIDTLEVLKAAGT
KWNFLPFRPGLVGGHCIGVDPYYLTYKADMLGYHPQVILAGRRINDGMGKYVAEQTVKQMIAAGSSIKGAKVVVLGLTFK
ENCPDLRNSKVIDVIRELQSYGCDVIVHDPVAAAAEAVHEYGVDLVAWDALPTAEAVVAAVSHREYLDMPLAQMLGKLKP
GGVFVDVKSAYDPAELTAAGARLWRL
>Mature_425_residues
TTIAVIGLGYVGLPLAVAFGKQFRTIGFDLSAEKVAAYKDFIDPTGEVSSEELKAAEHLSCHTDPAVLREADFVIVAVPT
PVDEAHNPDFTPLVKSSETVGRNLKPGAIVVYESTVYPGATEEVCIPILERESGLKWKEGFFVGYSPERINPGDKERTVT
KIVKVVSGDTPETLATVERVYGAVITAGVYPASSIKVAEAAKVIENTQRDLNIALMNELAVIFHKIGIDTLEVLKAAGTK
WNFLPFRPGLVGGHCIGVDPYYLTYKADMLGYHPQVILAGRRINDGMGKYVAEQTVKQMIAAGSSIKGAKVVVLGLTFKE
NCPDLRNSKVIDVIRELQSYGCDVIVHDPVAAAAEAVHEYGVDLVAWDALPTAEAVVAAVSHREYLDMPLAQMLGKLKPG
GVFVDVKSAYDPAELTAAGARLWRL

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG0677

COG function: function code M; UDP-N-acetyl-D-mannosaminuronate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-glucose/GDP-mannose dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI48994968, Length=415, Percent_Identity=31.566265060241, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI1788340, Length=366, Percent_Identity=23.7704918032787, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17560350, Length=462, Percent_Identity=23.1601731601732, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR017476
- InterPro:   IPR014027
- InterPro:   IPR014026
- InterPro:   IPR014028
- InterPro:   IPR001732 [H]

Pfam domain/function: PF00984 UDPG_MGDP_dh; PF03720 UDPG_MGDP_dh_C; PF03721 UDPG_MGDP_dh_N [H]

EC number: 1.1.1.- [C]

Molecular weight: Translated: 45924; Mature: 45793

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIAVIGLGYVGLPLAVAFGKQFRTIGFDLSAEKVAAYKDFIDPTGEVSSEELKAAEHL
CCEEEEEECCHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHC
SCHTDPAVLREADFVIVAVPTPVDEAHNPDFTPLVKSSETVGRNLKPGAIVVYESTVYPG
CCCCCHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHCCCCCCCEEEEEECCCCCC
ATEEVCIPILERESGLKWKEGFFVGYSPERINPGDKERTVTKIVKVVSGDTPETLATVER
CCHHHHHEEEECCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
VYGAVITAGVYPASSIKVAEAAKVIENTQRDLNIALMNELAVIFHKIGIDTLEVLKAAGT
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHCCC
KWNFLPFRPGLVGGHCIGVDPYYLTYKADMLGYHPQVILAGRRINDGMGKYVAEQTVKQM
CCCEECCCCCCCCCEEECCCCEEEEEEHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
IAAGSSIKGAKVVVLGLTFKENCPDLRNSKVIDVIRELQSYGCDVIVHDPVAAAAEAVHE
HHCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHH
YGVDLVAWDALPTAEAVVAAVSHREYLDMPLAQMLGKLKPGGVFVDVKSAYDPAELTAAG
CCCCEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCEEEEECCCCCCHHHHHHH
ARLWRL
HHHHCC
>Mature Secondary Structure 
TTIAVIGLGYVGLPLAVAFGKQFRTIGFDLSAEKVAAYKDFIDPTGEVSSEELKAAEHL
CEEEEEECCHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHC
SCHTDPAVLREADFVIVAVPTPVDEAHNPDFTPLVKSSETVGRNLKPGAIVVYESTVYPG
CCCCCHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHCCCCCCCEEEEEECCCCCC
ATEEVCIPILERESGLKWKEGFFVGYSPERINPGDKERTVTKIVKVVSGDTPETLATVER
CCHHHHHEEEECCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHH
VYGAVITAGVYPASSIKVAEAAKVIENTQRDLNIALMNELAVIFHKIGIDTLEVLKAAGT
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHCCC
KWNFLPFRPGLVGGHCIGVDPYYLTYKADMLGYHPQVILAGRRINDGMGKYVAEQTVKQM
CCCEECCCCCCCCCEEECCCCEEEEEEHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
IAAGSSIKGAKVVVLGLTFKENCPDLRNSKVIDVIRELQSYGCDVIVHDPVAAAAEAVHE
HHCCCCCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHH
YGVDLVAWDALPTAEAVVAAVSHREYLDMPLAQMLGKLKPGGVFVDVKSAYDPAELTAAG
CCCCEEEECCCCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCEEEEECCCCCCHHHHHHH
ARLWRL
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-acetyl-D-mannosamine; NAD [C]

Specific reaction: UDP-N-acetyl-D-mannosamine + NAD = UDP-N-acetyl-D-mannosaminuronic-acid + NADH + Proton [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]