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The map label for this gene is yjbJ [H]

Identifier: 119899478

GI number: 119899478

Start: 3500858

End: 3501787

Strand: Reverse

Name: yjbJ [H]

Synonym: azo3188

Alternate gene names: 119899478

Gene position: 3501787-3500858 (Counterclockwise)

Preceding gene: 119899479

Following gene: 119899477

Centisome position: 80.02

GC content: 67.2

Gene sequence:

>930_bases
ATGAAACCGCGCCCGCCTCTGCCGCTTGCCCGCCGGCATGCGCTGGCGGGCCTGATCGTCGCGTGTACAGCCGCCGCACA
GCCGGCGGCTGGCGCGGAAACCGGCCCCGCAGCCTCCGCCCGCCAAGGGCGCTACCTCGTCGAACAGGCACTGGCCTACG
AGCACGGCGAAGGGGTGAGGCGCGACCCGGAGCACGCGGCGGTGTTGTATTGCGAAGCCGCCCGCCTCGGCGACGCAGAA
GCGATGTACTCGCTGGGCTGGATGTACGCCAATGGGCGCGGCTTGGCGCGAAACGACGGCTACGCAGGGACGCTGTTCGC
GATGGCCGCGTTTCTCGGCAACGAGCACGCGGGCAAGTTGATGCGTTACACCGGCTACAGCGGGGCGGTACCCGATTGTC
TCCATCCGCCGCGTGACAGCATTCTGGATGACTGGCGTGCCGACGAGGCCATCGCCGCGATGTCGCCAGAACGGCAAAGG
GTGGCACGCTTGCTGGTTGAGCATGCGCCACGGTTCGGCATCCGACCGCGCTTCGCGCTCGCGATCGCGCTGGCCGAGTC
CAATCTCAACCCCAATGCGGTGTCACCGAAGAACGCGATGGGCGTGATGCAACTCATTCCCGAAACGGCGGAACGCTTCC
AGGTGCGCAAGCCCTTCGACGCCGAAGAAAACATCAAAGGCGGCCTGGCCTATCTCCGCTGGCTGCTGGCTTACTTTCGT
GGCGACATCGCGCTCGCTGCCGCCGCCTATAACGCCGGCGAGGGCAATGTCGACAAGTACCGCGGCGTTCCACCGTTCCG
CGAGACCCAGCGCTATGTGGAGCGCATTCTCGCGTTTGTCCGCACGCGCCAGCACCCGTTTGATACCCGCGTTGTCGACG
CCAGCCCGATGATCCAGGCGGTGCCGCCGCTGCCGGGGCGCGGCTCATGA

Upstream 100 bases:

>100_bases
AAGCCGGGAGGCGTGTTTGTCGACGTGAAGTCAGCCTACGACCCTGCCGAGCTCACAGCCGCAGGCGCGCGGCTGTGGCG
TCTGTAAGACGGCCGCGCTG

Downstream 100 bases:

>100_bases
TGCCGCTGCACGTCGGAACTGTCAGGCGCTTTCTCACCTCGGCGCTGGCGGTCCTGCTCTGTGCACTCACTGTCGCACCG
ACCGCACATGCCGATCTGGC

Product: soluble lytic murein transglycosylase

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKPRPPLPLARRHALAGLIVACTAAAQPAAGAETGPAASARQGRYLVEQALAYEHGEGVRRDPEHAAVLYCEAARLGDAE
AMYSLGWMYANGRGLARNDGYAGTLFAMAAFLGNEHAGKLMRYTGYSGAVPDCLHPPRDSILDDWRADEAIAAMSPERQR
VARLLVEHAPRFGIRPRFALAIALAESNLNPNAVSPKNAMGVMQLIPETAERFQVRKPFDAEENIKGGLAYLRWLLAYFR
GDIALAAAAYNAGEGNVDKYRGVPPFRETQRYVERILAFVRTRQHPFDTRVVDASPMIQAVPPLPGRGS

Sequences:

>Translated_309_residues
MKPRPPLPLARRHALAGLIVACTAAAQPAAGAETGPAASARQGRYLVEQALAYEHGEGVRRDPEHAAVLYCEAARLGDAE
AMYSLGWMYANGRGLARNDGYAGTLFAMAAFLGNEHAGKLMRYTGYSGAVPDCLHPPRDSILDDWRADEAIAAMSPERQR
VARLLVEHAPRFGIRPRFALAIALAESNLNPNAVSPKNAMGVMQLIPETAERFQVRKPFDAEENIKGGLAYLRWLLAYFR
GDIALAAAAYNAGEGNVDKYRGVPPFRETQRYVERILAFVRTRQHPFDTRVVDASPMIQAVPPLPGRGS
>Mature_309_residues
MKPRPPLPLARRHALAGLIVACTAAAQPAAGAETGPAASARQGRYLVEQALAYEHGEGVRRDPEHAAVLYCEAARLGDAE
AMYSLGWMYANGRGLARNDGYAGTLFAMAAFLGNEHAGKLMRYTGYSGAVPDCLHPPRDSILDDWRADEAIAAMSPERQR
VARLLVEHAPRFGIRPRFALAIALAESNLNPNAVSPKNAMGVMQLIPETAERFQVRKPFDAEENIKGGLAYLRWLLAYFR
GDIALAAAAYNAGEGNVDKYRGVPPFRETQRYVERILAFVRTRQHPFDTRVVDASPMIQAVPPLPGRGS

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=125, Percent_Identity=34.4, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 33705; Mature: 33705

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPRPPLPLARRHALAGLIVACTAAAQPAAGAETGPAASARQGRYLVEQALAYEHGEGVR
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHCCCCCC
RDPEHAAVLYCEAARLGDAEAMYSLGWMYANGRGLARNDGYAGTLFAMAAFLGNEHAGKL
CCCCCEEEEEEEHHHCCCHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHHCCHHHCCC
MRYTGYSGAVPDCLHPPRDSILDDWRADEAIAAMSPERQRVARLLVEHAPRFGIRPRFAL
EEECCCCCCCCCCCCCCHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHE
AIALAESNLNPNAVSPKNAMGVMQLIPETAERFQVRKPFDAEENIKGGLAYLRWLLAYFR
EEEEHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
GDIALAAAAYNAGEGNVDKYRGVPPFRETQRYVERILAFVRTRQHPFDTRVVDASPMIQA
CCHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHC
VPPLPGRGS
CCCCCCCCC
>Mature Secondary Structure
MKPRPPLPLARRHALAGLIVACTAAAQPAAGAETGPAASARQGRYLVEQALAYEHGEGVR
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHCCCCCC
RDPEHAAVLYCEAARLGDAEAMYSLGWMYANGRGLARNDGYAGTLFAMAAFLGNEHAGKL
CCCCCEEEEEEEHHHCCCHHHHHHHHEEEECCCCCCCCCCCHHHHHHHHHHHCCHHHCCC
MRYTGYSGAVPDCLHPPRDSILDDWRADEAIAAMSPERQRVARLLVEHAPRFGIRPRFAL
EEECCCCCCCCCCCCCCHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHE
AIALAESNLNPNAVSPKNAMGVMQLIPETAERFQVRKPFDAEENIKGGLAYLRWLLAYFR
EEEEHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
GDIALAAAAYNAGEGNVDKYRGVPPFRETQRYVERILAFVRTRQHPFDTRVVDASPMIQA
CCHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHC
VPPLPGRGS
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]